AR005035A1 - Procedimiento para preparar proteinas recombinantes en e. coli, mediante fermentacion con gran concentracion de celulas. - Google Patents

Procedimiento para preparar proteinas recombinantes en e. coli, mediante fermentacion con gran concentracion de celulas.

Info

Publication number
AR005035A1
AR005035A1 ARP960105609A ARP960105609A AR005035A1 AR 005035 A1 AR005035 A1 AR 005035A1 AR P960105609 A ARP960105609 A AR P960105609A AR P960105609 A ARP960105609 A AR P960105609A AR 005035 A1 AR005035 A1 AR 005035A1
Authority
AR
Argentina
Prior art keywords
recombinant proteins
coli
cells
fermentation
procedure
Prior art date
Application number
ARP960105609A
Other languages
English (en)
Original Assignee
Merck Patent Ges Mit Beschränkter Haftung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Ges Mit Beschränkter Haftung filed Critical Merck Patent Ges Mit Beschränkter Haftung
Publication of AR005035A1 publication Critical patent/AR005035A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Abstract

El invento se refiere a un procedimiento de fermentacion de fedbatch con sistemas especiales de vector-hospedante para la formacion eficaz de proteinasrecombinantes, en particular de moleculas de anticuerpos recombinantes, preferentementede fragmentos de anticuerpos, tales como minianticuerpos. Enlas condiciones dadas, las celulas de E. coli pueden crecer con velocidad especifica maxima hasta alcanzar concentraciones muy grandes. Una vez iniciadalaf ormacion del productorecomb inante, solamente el producto formado actua limitando el crecimiento; no tiene lugar limitacion alguna del crecimiento porparte del sustrato o de productos metabolicos secundarios. De este modo se pueden lograr grandes rendimientosde protei nas recombinante, en relacion conel volumen y el tiempo.
ARP960105609A 1995-12-11 1996-11-12 Procedimiento para preparar proteinas recombinantes en e. coli, mediante fermentacion con gran concentracion de celulas. AR005035A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP95119478 1995-12-11

Publications (1)

Publication Number Publication Date
AR005035A1 true AR005035A1 (es) 1999-04-07

Family

ID=8219878

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP960105609A AR005035A1 (es) 1995-12-11 1996-11-12 Procedimiento para preparar proteinas recombinantes en e. coli, mediante fermentacion con gran concentracion de celulas.

Country Status (24)

Country Link
US (1) US6410270B1 (es)
EP (1) EP0866876B1 (es)
JP (2) JP4101879B2 (es)
KR (1) KR100444735B1 (es)
CN (1) CN1117160C (es)
AR (1) AR005035A1 (es)
AT (1) ATE213271T1 (es)
AU (1) AU716612B2 (es)
BR (1) BR9611937A (es)
CA (1) CA2240097C (es)
CZ (1) CZ291415B6 (es)
DE (1) DE59608743D1 (es)
DK (1) DK0866876T3 (es)
ES (1) ES2171752T3 (es)
HU (1) HU227945B1 (es)
NO (1) NO319090B1 (es)
PL (1) PL185665B1 (es)
PT (1) PT866876E (es)
RU (1) RU2201455C2 (es)
SI (1) SI0866876T1 (es)
SK (1) SK283169B6 (es)
UA (1) UA61066C2 (es)
WO (1) WO1997021829A1 (es)
ZA (1) ZA9610384B (es)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9715895D0 (en) 1997-07-29 1997-10-01 Zeneca Ltd Heterocyclic compounds
US7858339B1 (en) 1998-10-28 2010-12-28 Genentech, Inc. Process for bacterial production of polypeptides
JP4878724B2 (ja) 2000-11-03 2012-02-15 ジェネンテック， インコーポレイテッド 組み換えタンパク質を発現する発酵における代謝速度シフト
WO2003004698A2 (en) * 2001-07-06 2003-01-16 Merck Patent Gmbh Method for monitoring and modulating protein folding
EP1706490A4 (en) * 2003-12-12 2008-02-27 Conjugon Inc STRICTLY REGULATED GENE EXPRESSION SYSTEMS
EP1704229B1 (en) * 2003-12-23 2010-05-19 Council Of Scientific And Industrial Research Process for producing streptokinase using genetically modified escherichia coli
ZA200704934B (en) 2004-12-22 2010-09-29 Genentech Inc Methods for producing soluble multi-membrane-spanning proteins
BRPI0715360B8 (pt) * 2006-07-27 2022-01-04 Wyeth Corp método para a produção de uma proteína recombinante; método para a produção de uma proteína 2086 meningocócica recombinante (p2086); e composição
CN103555652A (zh) 2006-09-13 2014-02-05 Abbvie公司 细胞培养改良
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
EP2326720B1 (en) 2008-09-15 2018-03-21 F. Hoffmann-La Roche AG Compositions and methods for regulating cell osmolarity
WO2011013721A1 (ja) * 2009-07-28 2011-02-03 三井化学株式会社 乳酸製造方法
CA2788607C (en) * 2010-02-01 2018-03-20 Digna Biotech,S.L. Process for the production of interferon alpha 5
MX339227B (es) 2010-08-30 2016-05-17 Hoffmann La Roche Alimentacion alcalina.
KR101252150B1 (ko) * 2010-11-18 2013-04-08 한국과학기술원 재조합 ＤＮＡ 분자 클로닝을 위한 알데히드 환원효소 ＹｑｈＤ 유전자를 포함하는 자살 벡터
RU2507736C2 (ru) * 2011-01-11 2014-02-27 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук (ИБГ РАН) Способ создания трансгенных растений с высоким уровнем экспрессии трансгенного белка
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
RU2496877C2 (ru) * 2011-12-15 2013-10-27 Общество с ограниченной ответственностью "Лаборатория медицинской биотехнологии" (ООО "ЛМБТ") ПЛАЗМИДНЫЙ ВЕКТОР pHYP С ПОВЫШЕННОЙ СЕГРЕГАЦИОННОЙ СТАБИЛЬНОСТЬЮ ДЛЯ ЭКСПРЕССИИ РЕКОМБИНАНТНОГО БЕЛКА, БАКТЕРИЯ - ПРОДУЦЕНТ ПРЕДШЕСТВЕННИКА РЕКОМБИНАНТНОГО БЕЛКА И СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОГО БЕЛКА
KR102036671B1 (ko) 2012-03-12 2019-11-26 한미사이언스 주식회사 대장균의 고농도 배양방법
CN102604904B (zh) * 2012-03-14 2013-09-18 苏州汉酶生物技术有限公司 一种葡萄糖脱氢酶的生产方法
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9505833B2 (en) 2012-04-20 2016-11-29 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
AU2013309506A1 (en) 2012-09-02 2015-03-12 Abbvie Inc. Methods to control protein heterogeneity
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
AU2014226126B2 (en) 2013-03-08 2019-02-14 Genzyme Corporation Continuous purification of therapeutic proteins
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
WO2015051293A2 (en) 2013-10-04 2015-04-09 Abbvie, Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
TWI709570B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析法及製法
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
US9714273B2 (en) * 2014-10-08 2017-07-25 Utah State University Expression systems and associated methods
MA45489A (fr) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh Procédés de culture de cellules, kits et appareil associés
WO2018197949A1 (en) 2017-04-27 2018-11-01 Juno Therapeutics Gmbh Oligomeric particle reagents and methods of use thereof
KR20210049155A (ko) 2018-08-31 2021-05-04 젠자임 코포레이션 멸균 크로마토그래피 수지 및 제조 공정에서 이의 용도
CN110684101A (zh) * 2019-11-15 2020-01-14 上海松皓生物科技有限公司 一种重组水蛭素的制备方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0531472B1 (en) * 1991-03-06 2003-08-13 MERCK PATENT GmbH Humanized monoclonal antibodies

Also Published As

Publication number Publication date
DE59608743D1 (de) 2002-03-21
JP2000501936A (ja) 2000-02-22
AU716612B2 (en) 2000-03-02
NO319090B1 (no) 2005-06-20
CA2240097C (en) 2005-07-26
PL327188A1 (en) 1998-11-23
SK74098A3 (en) 1998-11-04
EP0866876A1 (de) 1998-09-30
RU2201455C2 (ru) 2003-03-27
KR100444735B1 (ko) 2004-11-10
HUP0000317A2 (hu) 2000-06-28
DK0866876T3 (da) 2002-05-27
CA2240097A1 (en) 1997-06-19
JP4101879B2 (ja) 2008-06-18
AU1032597A (en) 1997-07-03
PT866876E (pt) 2002-07-31
BR9611937A (pt) 1999-03-02
CN1219203A (zh) 1999-06-09
KR19990072037A (ko) 1999-09-27
ES2171752T3 (es) 2002-09-16
WO1997021829A1 (de) 1997-06-19
SI0866876T1 (en) 2002-08-31
CZ165298A3 (cs) 1998-08-12
ATE213271T1 (de) 2002-02-15
HU227945B1 (en) 2012-06-28
SK283169B6 (sk) 2003-03-04
HUP0000317A3 (en) 2001-09-28
NO982672L (no) 1998-06-10
EP0866876B1 (de) 2002-02-13
NO982672D0 (no) 1998-06-10
US6410270B1 (en) 2002-06-25
JP2007185197A (ja) 2007-07-26
ZA9610384B (en) 1997-06-23
PL185665B1 (pl) 2003-06-30
UA61066C2 (en) 2003-11-17
JP4102422B2 (ja) 2008-06-18
CZ291415B6 (cs) 2003-03-12
CN1117160C (zh) 2003-08-06

Similar Documents

Publication Publication Date Title
AR005035A1 (es) Procedimiento para preparar proteinas recombinantes en e. coli, mediante fermentacion con gran concentracion de celulas.
RU94045287A (ru) Устойчивый, высококонцентрированный иммуноглобулиновый препарат, пригодный для внутривенного введения и способ его получения
CN105431454A (zh) 用于生产单克隆抗体的改进方法
AR248426A1 (es) Produccion de particulas incorporadas a un virus vegetal que contiene un peptido antigenico, con el fin de preparar una vacuna de virus animal.
ES2188190T3 (es) Producto alimentario.
ES2095565T3 (es) Producto estable en almacen y proceso para aislar rna, dna y proteinas.
ATE492642T1 (de) Zirkuläres dna molekül mit einem abhängigen replikationsursprung, seine herstellungsverfahren und seine verwendung in der gentherapie
AR076607A1 (es) Composicion acuosa. metodo para producir una preparacion altamente concentrada de inmunoglobulina de uso subcutaneo
ATE334218T1 (de) Verfahren zur gleichzeitigen herstellung von xylitol und ethanol
DK0506866T3 (da) Fremgangsmåde til krystallisation af enzymer
ES2109349T3 (es) Gel ionotropo deficiente en entidad ionica de gelificacion, procedimiento de preparacion de tal gel y utilizacion de este en particular en un procedimiento de elaboracion de vino efervescente.
SE9402595D0 (sv) Förfarande för kristallisation av iopamidol
CN104593319A (zh) 一种用于细胞培养基的小麦活性肽添加剂
KR870004130A (ko) 재조합균세포의 배양방법
ATE127520T1 (de) Protein mit transaktivierender eigenschaft, dieses protein exprimierende vektoren, zellinien und seine verwendungen.
KR880012757A (ko) 인간 소마토메딘 c의 고역가 생산방법
AR240783A2 (es) Procedimiento de esterilizacion de materiales de embalaje para el envasado aseptico de jugos de fruta y vino
SU881114A1 (ru) Способ предотвращени полусладких виноградных вин от забраживани
GB1473037A (en) Process for the preservation of a foodstuff
AR007624A1 (es) Procedimiento para la fabricacion de un producto biologico, estabilizante de las precipitaciones proteicas de los vinos blancos, el producto asiobtenido, el procedimiento de seleccion de levaduras para su aplicacion en dicho procedimiento y el procedimiento de tratamiento de los vinos blancos con
RU93028311A (ru) Способ получения биологически активных веществ из биомассы культивируемых клеток растений
GB1107693A (en) Flavourings and seasonings
Bakratsas Production of high-value nutritional ingredients by submerged fermentation of Pleurotus ostreatus
SU485142A1 (ru) Способ выращивани микроорганизмов
CN117778192A (zh) 一种激光培养藻类的方法

Legal Events

Date Code Title Description
FB Suspension of granting procedure