NO982672D0 - FremgangsmÕte for fremstilling av rekombinante proteiner i E. coli ved fermentering med h°y celletetthet - Google Patents

FremgangsmÕte for fremstilling av rekombinante proteiner i E. coli ved fermentering med h°y celletetthet

Info

Publication number
NO982672D0
NO982672D0 NO982672A NO982672A NO982672D0 NO 982672 D0 NO982672 D0 NO 982672D0 NO 982672 A NO982672 A NO 982672A NO 982672 A NO982672 A NO 982672A NO 982672 D0 NO982672 D0 NO 982672D0
Authority
NO
Norway
Prior art keywords
coli
fermentation
preparation
cell density
recombinant proteins
Prior art date
Application number
NO982672A
Other languages
English (en)
Other versions
NO982672L (no
NO319090B1 (no
Inventor
Wolfgang Strittmatter
Siegfried Matzku
Dieter Riesenberg
Uwe Horn
Uwe Knuepfer
Marian Kujau
Rolf Wenderoth
Andreas Plueckthun
Anke Krebber
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Publication of NO982672D0 publication Critical patent/NO982672D0/no
Publication of NO982672L publication Critical patent/NO982672L/no
Publication of NO319090B1 publication Critical patent/NO319090B1/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
NO19982672A 1995-12-11 1998-06-10 Fremgangsmate for fremstilling av rekombinante proteiner i E. coli ved fermentering med hoy celletetthet, E. coli-ekspresjonsvektor og transformert E. coli-ekspresjonsvert. NO319090B1 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP95119478 1995-12-11
PCT/EP1996/005260 WO1997021829A1 (de) 1995-12-11 1996-11-28 Verfahren zur herstellung von rekombinanten proteinen in e. coli mittels hochzelldichte-fermentation

Publications (3)

Publication Number Publication Date
NO982672D0 true NO982672D0 (no) 1998-06-10
NO982672L NO982672L (no) 1998-06-10
NO319090B1 NO319090B1 (no) 2005-06-20

Family

ID=8219878

Family Applications (1)

Application Number Title Priority Date Filing Date
NO19982672A NO319090B1 (no) 1995-12-11 1998-06-10 Fremgangsmate for fremstilling av rekombinante proteiner i E. coli ved fermentering med hoy celletetthet, E. coli-ekspresjonsvektor og transformert E. coli-ekspresjonsvert.

Country Status (24)

Country Link
US (1) US6410270B1 (no)
EP (1) EP0866876B1 (no)
JP (2) JP4101879B2 (no)
KR (1) KR100444735B1 (no)
CN (1) CN1117160C (no)
AR (1) AR005035A1 (no)
AT (1) ATE213271T1 (no)
AU (1) AU716612B2 (no)
BR (1) BR9611937A (no)
CA (1) CA2240097C (no)
CZ (1) CZ291415B6 (no)
DE (1) DE59608743D1 (no)
DK (1) DK0866876T3 (no)
ES (1) ES2171752T3 (no)
HU (1) HU227945B1 (no)
NO (1) NO319090B1 (no)
PL (1) PL185665B1 (no)
PT (1) PT866876E (no)
RU (1) RU2201455C2 (no)
SI (1) SI0866876T1 (no)
SK (1) SK283169B6 (no)
UA (1) UA61066C2 (no)
WO (1) WO1997021829A1 (no)
ZA (1) ZA9610384B (no)

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GB9715895D0 (en) 1997-07-29 1997-10-01 Zeneca Ltd Heterocyclic compounds
AU1223600A (en) 1998-10-28 2000-05-15 Genentech Inc. Process
WO2002040697A2 (en) 2000-11-03 2002-05-23 Genentech, Inc. Metabolic rate shifts in fermentations expressing recombinant proteins
DE60230147D1 (de) * 2001-07-06 2009-01-15 Merck Patent Gmbh Verfahren zur überwachung und modulierung von proteinfaltung
CA2549263A1 (en) * 2003-12-12 2005-08-11 Conjugon, Inc. Systems for tightly regulated gene expression
ATE468392T1 (de) * 2003-12-23 2010-06-15 Council Scient Ind Res Verfahren zur herstellung von streptokinase durch kultivierung von genetisch veränderten escherichia coli
EP2290088A1 (en) 2004-12-22 2011-03-02 Genentech, Inc. Methods for producing soluble multi-menbrane-spanning proteins
JP2009544321A (ja) * 2006-07-27 2009-12-17 ワイス 組換えタンパク質を製造するための高細胞密度流加発酵法
CN103397065A (zh) 2006-09-13 2013-11-20 雅培制药有限公司 细胞培养改良
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
KR20110056323A (ko) 2008-09-15 2011-05-26 제넨테크, 인크. 세포 오스몰농도를 조절하기 위한 조성물 및 방법
CN102471787B (zh) * 2009-07-28 2016-01-20 三井化学株式会社 乳酸制造方法
KR101858598B1 (ko) * 2010-02-01 2018-05-16 디그나 비오테크, 에스.엘. 인터페론 알파 5의 제조방법
US8951756B2 (en) * 2010-08-30 2015-02-10 Hoffmann-La Roche Inc. Alkaline feed
KR101252150B1 (ko) * 2010-11-18 2013-04-08 한국과학기술원 재조합 ＤＮＡ 분자 클로닝을 위한 알데히드 환원효소 ＹｑｈＤ 유전자를 포함하는 자살 벡터
RU2507736C2 (ru) * 2011-01-11 2014-02-27 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук (ИБГ РАН) Способ создания трансгенных растений с высоким уровнем экспрессии трансгенного белка
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
RU2496877C2 (ru) * 2011-12-15 2013-10-27 Общество с ограниченной ответственностью "Лаборатория медицинской биотехнологии" (ООО "ЛМБТ") ПЛАЗМИДНЫЙ ВЕКТОР pHYP С ПОВЫШЕННОЙ СЕГРЕГАЦИОННОЙ СТАБИЛЬНОСТЬЮ ДЛЯ ЭКСПРЕССИИ РЕКОМБИНАНТНОГО БЕЛКА, БАКТЕРИЯ - ПРОДУЦЕНТ ПРЕДШЕСТВЕННИКА РЕКОМБИНАНТНОГО БЕЛКА И СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОГО БЕЛКА
EP2817625A2 (en) 2012-02-23 2014-12-31 Stage Cell Therapeutics GmbH Chromatographic isolation of cells and other complex biological materials
PT2825633T (pt) 2012-03-12 2019-06-06 Hanmi Science Co Ltd Método para cultivar células de e. coli para alta densidade
CN102604904B (zh) * 2012-03-14 2013-09-18 苏州汉酶生物技术有限公司 一种葡萄糖脱氢酶的生产方法
US9505833B2 (en) 2012-04-20 2016-11-29 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
WO2014035475A1 (en) 2012-09-02 2014-03-06 Abbvie Inc. Methods to control protein heterogeneity
SG10201709131UA (en) 2013-03-08 2017-12-28 Genzyme Corp Continuous purification of therapeutic proteins
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
TWI671312B (zh) 2014-01-17 2019-09-11 美商健臻公司 無菌層析法及製法
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
US9714273B2 (en) * 2014-10-08 2017-07-25 Utah State University Expression systems and associated methods
MA45489A (fr) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh Procédés de culture de cellules, kits et appareil associés
TW201842335A (zh) 2017-04-27 2018-12-01 德商朱諾醫療公司 寡聚粒子試劑及其使用方法
CN112638488A (zh) 2018-08-31 2021-04-09 建新公司 灭菌的层析树脂及其在制造工艺中的用途
CN110684101A (zh) * 2019-11-15 2020-01-14 上海松皓生物科技有限公司 一种重组水蛭素的制备方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ282603B6 (cs) * 1991-03-06 1997-08-13 Merck Patent Gesellschaft Mit Beschränkter Haftun G Humanizované a chimerické monoklonální protilátky

Also Published As

Publication number Publication date
PL185665B1 (pl) 2003-06-30
WO1997021829A1 (de) 1997-06-19
PT866876E (pt) 2002-07-31
PL327188A1 (en) 1998-11-23
EP0866876A1 (de) 1998-09-30
JP4102422B2 (ja) 2008-06-18
DE59608743D1 (de) 2002-03-21
HUP0000317A3 (en) 2001-09-28
JP2007185197A (ja) 2007-07-26
AR005035A1 (es) 1999-04-07
CZ165298A3 (cs) 1998-08-12
CA2240097A1 (en) 1997-06-19
ATE213271T1 (de) 2002-02-15
JP2000501936A (ja) 2000-02-22
AU716612B2 (en) 2000-03-02
HU227945B1 (en) 2012-06-28
CN1219203A (zh) 1999-06-09
AU1032597A (en) 1997-07-03
SI0866876T1 (en) 2002-08-31
ES2171752T3 (es) 2002-09-16
BR9611937A (pt) 1999-03-02
HUP0000317A2 (hu) 2000-06-28
NO982672L (no) 1998-06-10
SK283169B6 (sk) 2003-03-04
SK74098A3 (en) 1998-11-04
US6410270B1 (en) 2002-06-25
KR100444735B1 (ko) 2004-11-10
JP4101879B2 (ja) 2008-06-18
UA61066C2 (en) 2003-11-17
NO319090B1 (no) 2005-06-20
DK0866876T3 (da) 2002-05-27
CA2240097C (en) 2005-07-26
CN1117160C (zh) 2003-08-06
RU2201455C2 (ru) 2003-03-27
ZA9610384B (en) 1997-06-23
EP0866876B1 (de) 2002-02-13
CZ291415B6 (cs) 2003-03-12
KR19990072037A (ko) 1999-09-27

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