SE0400886D0 - Process of purification - Google Patents

Process of purification

Info

Publication number
SE0400886D0
SE0400886D0 SE0400886A SE0400886A SE0400886D0 SE 0400886 D0 SE0400886 D0 SE 0400886D0 SE 0400886 A SE0400886 A SE 0400886A SE 0400886 A SE0400886 A SE 0400886A SE 0400886 D0 SE0400886 D0 SE 0400886D0
Authority
SE
Sweden
Prior art keywords
chromatography matrix
target compound
ionic polyether
peg
isolating
Prior art date
Application number
SE0400886A
Other languages
English (en)
Inventor
James Van Alstine
Anders Ljungloef
Hamid Houshmand
Per-Mikael Aaberg
Original Assignee
Amersham Biosciences Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Ab filed Critical Amersham Biosciences Ab
Priority to SE0400886A priority Critical patent/SE0400886D0/sv
Publication of SE0400886D0 publication Critical patent/SE0400886D0/sv
Priority to AT05722292T priority patent/ATE485880T1/de
Priority to DE602005024373T priority patent/DE602005024373D1/de
Priority to US10/594,187 priority patent/US20070213513A1/en
Priority to AU2005228834A priority patent/AU2005228834A1/en
Priority to PCT/SE2005/000467 priority patent/WO2005094960A1/en
Priority to CA002560012A priority patent/CA2560012A1/en
Priority to EP05722292A priority patent/EP1729867B1/en
Priority to JP2007506111A priority patent/JP4831697B2/ja

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
  • Polyethers (AREA)
SE0400886A 2004-04-02 2004-04-02 Process of purification SE0400886D0 (sv)

Priority Applications (9)

Application Number Priority Date Filing Date Title
SE0400886A SE0400886D0 (sv) 2004-04-02 2004-04-02 Process of purification
AT05722292T ATE485880T1 (de) 2004-04-02 2005-03-31 Verfahren zur chromatographischen reinigung
DE602005024373T DE602005024373D1 (de) 2004-04-02 2005-03-31 Verfahren zur chromatographischen reinigung
US10/594,187 US20070213513A1 (en) 2004-04-02 2005-03-31 Method for Chromatographic Purification
AU2005228834A AU2005228834A1 (en) 2004-04-02 2005-03-31 A method for chromatographic purification
PCT/SE2005/000467 WO2005094960A1 (en) 2004-04-02 2005-03-31 A method for chromatographic purification
CA002560012A CA2560012A1 (en) 2004-04-02 2005-03-31 A method for chromatographic purification
EP05722292A EP1729867B1 (en) 2004-04-02 2005-03-31 A method for chromatographic purification
JP2007506111A JP4831697B2 (ja) 2004-04-02 2005-03-31 精製方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE0400886A SE0400886D0 (sv) 2004-04-02 2004-04-02 Process of purification

Publications (1)

Publication Number Publication Date
SE0400886D0 true SE0400886D0 (sv) 2004-04-02

Family

ID=32173667

Family Applications (1)

Application Number Title Priority Date Filing Date
SE0400886A SE0400886D0 (sv) 2004-04-02 2004-04-02 Process of purification

Country Status (9)

Country Link
US (1) US20070213513A1 (sv)
EP (1) EP1729867B1 (sv)
JP (1) JP4831697B2 (sv)
AT (1) ATE485880T1 (sv)
AU (1) AU2005228834A1 (sv)
CA (1) CA2560012A1 (sv)
DE (1) DE602005024373D1 (sv)
SE (1) SE0400886D0 (sv)
WO (1) WO2005094960A1 (sv)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW201516149A (zh) 2006-09-13 2015-05-01 Abbvie Inc 細胞培養改良
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
US8268915B2 (en) * 2007-06-19 2012-09-18 Ge Healthcare Bio-Sciences Ab Polymer two phase system and use thereof
CA2711375C (en) * 2008-01-18 2019-04-16 F. Hoffmann-La Roche Ag Purification of not-glycosylated polypeptides
JP5596560B2 (ja) * 2008-02-05 2014-09-24 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ 分離媒体の製造方法
FR2930780B1 (fr) * 2008-04-30 2010-08-20 Moet & Chandon Procede de degorgement de vins mousseux
CN102119168A (zh) * 2008-06-03 2011-07-06 帕特里有限公司 抗体纯化工艺
JP5808249B2 (ja) * 2008-10-20 2015-11-10 アッヴィ・インコーポレイテッド プロテインａアフィニティークロマトグラフィーを用いる抗体の単離および精製
CN104974251A (zh) 2008-10-20 2015-10-14 Abbvie公司 在抗体纯化过程中的病毒灭活
SG193378A1 (en) 2011-03-16 2013-10-30 Hoffmann La Roche Ion exchange chromatography with improved selectivity for the separation of polypeptide monomers, aggregates and fragments by modulation of the mobile phase
SG193545A1 (en) 2011-03-29 2013-11-29 Glaxosmithkline Llc Buffer system for protein purification
CN103717284B (zh) * 2011-06-08 2016-02-24 新加坡科技研究局 通过约束共水合色谱纯化生物制品
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
EP3048109A4 (en) * 2013-09-17 2017-04-19 Kaneka Corporation Novel antibody purification method and antibody obtained therefrom, and novel antibody purification method using cation exchanger and antibody obtained therefrom
MY196321A (en) 2016-08-16 2023-03-24 Regeneron Pharma Methods for Quantitating Individual Antibodies From a Mixture
WO2018081203A1 (en) 2016-10-25 2018-05-03 Regeneron Pharmaceuticals, Inc. Methods and systems for chromatography data analysis
CN108152104B (zh) * 2017-12-25 2020-08-11 上海微谱化工技术服务有限公司 超支化水性聚氨酯中二氧化硅填料的分离及定量方法
CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
US11420916B2 (en) * 2018-06-08 2022-08-23 Board Of Regents, The University Of Texas System Systems and methods for separation of olefins from mixtures that contain reducing agents
TW202005694A (zh) 2018-07-02 2020-02-01 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
KR20220078634A (ko) * 2019-10-04 2022-06-10 메르크 파텐트 게엠베하 단백질 a 친화성 크로마토그래피에서 모노클로날 항체의 용리

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3869436A (en) * 1971-06-01 1975-03-04 Statens Bakteriologiska Lab Method for fractionating plasma proteins using peg and ion-exchangers
EP0119767B1 (en) * 1983-03-11 1990-11-22 FUJIREBIO KABUSHIKI KAISHA also trading as FUJIREBIO INC. Method of measuring ligands
US4683294A (en) * 1985-04-03 1987-07-28 Smith Kline Rit, S.A. Process for the extraction and purification of proteins from culture media producing them
ES2052602T3 (es) * 1986-10-03 1994-07-16 Ciba Geigy Ag Nuevos peptidos afines a las linfocinas.
US5151358A (en) * 1989-06-13 1992-09-29 Genencor International, Inc. Processes for the recovery of naturally produced chymosin
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
US5443953A (en) * 1993-12-08 1995-08-22 Immunomedics, Inc. Preparation and use of immunoconjugates
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
SE9503925D0 (sv) * 1995-11-07 1995-11-07 Pharmacia Biotech Ab Separationsmedium för IgG
US20020006405A1 (en) * 1996-10-22 2002-01-17 Masaki Kitajima Sepsis remedy comprising anti-il-8 antibody as active ingredient
AUPR110100A0 (en) * 2000-10-30 2000-11-23 Life Therapeutics Limited Improved separation of macromolecules
US7514078B2 (en) * 2001-06-01 2009-04-07 Cornell Research Foundation, Inc. Methods of treating prostate cancer with anti-prostate specific membrane antigen antibodies
US7998705B2 (en) * 2002-08-06 2011-08-16 FUJIFILM Diosynth Biotechnologies U.S.A., Inc Increased dynamic binding capacity in ion exchange chromatography by addition of polyethylene glycol
US20060275766A1 (en) * 2003-01-07 2006-12-07 Haurum John S Method for manufacturing recombinant polyclonal proteins

Also Published As

Publication number Publication date
JP2007530971A (ja) 2007-11-01
US20070213513A1 (en) 2007-09-13
AU2005228834A1 (en) 2005-10-13
WO2005094960A1 (en) 2005-10-13
JP4831697B2 (ja) 2011-12-07
ATE485880T1 (de) 2010-11-15
DE602005024373D1 (de) 2010-12-09
CA2560012A1 (en) 2005-10-13
EP1729867B1 (en) 2010-10-27
EP1729867A1 (en) 2006-12-13

Similar Documents

Publication Publication Date Title
ATE485880T1 (de) Verfahren zur chromatographischen reinigung
Martosella et al. Reversed-phase high-performance liquid chromatographic prefractionation of immunodepleted human serum proteins to enhance mass spectrometry identification of lower-abundant proteins
Gilar et al. Orthogonality of separation in two-dimensional liquid chromatography
Gilar et al. Characterization of therapeutic oligonucleotides using liquid chromatography with on-line mass spectrometry detection
DK1725581T3 (da) Ny assay til adskillelse og kvantificering af hæmagglutinin-antigener
Gong Comparing ion‐pairing reagents and counter anions for ion‐pair reversed‐phase liquid chromatography/electrospray ionization mass spectrometry analysis of synthetic oligonucleotides
JP2007530971A5 (sv)
ATE428728T1 (de) Verfahren zur reinigung von erythropoietin
ATE440858T1 (de) Aufreinigung von antikírpern
Geiser et al. In-line system containing porous polymer monoliths for protein digestion with immobilized pepsin, peptide preconcentration and nano-liquid chromatography separation coupled to electrospray ionization mass spectroscopy
DK1809663T3 (da) Fremgangsmåde til oprensning af FSH
WO2007081906A3 (en) Purification by column-chromatography using eluants containing organic solvents
ATE337058T1 (de) Kolonne und mit dieser durchgeführtes chromatographisches verfahren
BRPI0513526A (pt) componentes de aparelho e métodos de utilizar componentes de aparelho para detectar a presença de um analisado
Tsay et al. Generic anion-exchange chromatography method for analytical and preparative separation of nucleotides in the development and manufacture of drug substances
Ghesquière et al. Proteome-wide characterization of N-glycosylation events by diagonal chromatography
WO2006118615A3 (en) Clonable tag for purification and electron microscopy labeling
WO2009139601A3 (en) Method and affinity column for purifying proteins
GB2427374A (en) Compositions and methods for separating enantiomers
BRPI0606168A2 (pt) separação de isÈmeros de fulvestrant
DE602004013040D1 (de) Aufreinigung von immunglobulinen
SE0401665D0 (sv) Purification of immunoglobulins
SE0302509D0 (sv) Matrix for separation of polyethers and method of separation
Petrotchenko et al. “Out-gel” tryptic digestion procedure for chemical cross-linking studies with mass spectrometric detection
SE0402558D0 (sv) A process for the purification of antibodies