DE602005024373D1 - Verfahren zur chromatographischen reinigung - Google Patents
Verfahren zur chromatographischen reinigungInfo
- Publication number
- DE602005024373D1 DE602005024373D1 DE602005024373T DE602005024373T DE602005024373D1 DE 602005024373 D1 DE602005024373 D1 DE 602005024373D1 DE 602005024373 T DE602005024373 T DE 602005024373T DE 602005024373 T DE602005024373 T DE 602005024373T DE 602005024373 D1 DE602005024373 D1 DE 602005024373D1
- Authority
- DE
- Germany
- Prior art keywords
- chromatography matrix
- target compound
- ionic polyether
- chromatographic
- peg
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
- B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
- B01D15/426—Specific type of solvent
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Peptides Or Proteins (AREA)
- Polyethers (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE0400886A SE0400886D0 (sv) | 2004-04-02 | 2004-04-02 | Process of purification |
PCT/SE2005/000467 WO2005094960A1 (en) | 2004-04-02 | 2005-03-31 | A method for chromatographic purification |
Publications (1)
Publication Number | Publication Date |
---|---|
DE602005024373D1 true DE602005024373D1 (de) | 2010-12-09 |
Family
ID=32173667
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE602005024373T Active DE602005024373D1 (de) | 2004-04-02 | 2005-03-31 | Verfahren zur chromatographischen reinigung |
Country Status (9)
Country | Link |
---|---|
US (1) | US20070213513A1 (de) |
EP (1) | EP1729867B1 (de) |
JP (1) | JP4831697B2 (de) |
AT (1) | ATE485880T1 (de) |
AU (1) | AU2005228834A1 (de) |
CA (1) | CA2560012A1 (de) |
DE (1) | DE602005024373D1 (de) |
SE (1) | SE0400886D0 (de) |
WO (1) | WO2005094960A1 (de) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008033517A2 (en) | 2006-09-13 | 2008-03-20 | Abbott Laboratories | Cell culture improvements |
US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
US8268915B2 (en) * | 2007-06-19 | 2012-09-18 | Ge Healthcare Bio-Sciences Ab | Polymer two phase system and use thereof |
KR20100103595A (ko) * | 2008-01-18 | 2010-09-27 | 에프. 호프만-라 로슈 아게 | 비-글라이코실화된 단백질의 정제 |
JP5596560B2 (ja) * | 2008-02-05 | 2014-09-24 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | 分離媒体の製造方法 |
FR2930780B1 (fr) * | 2008-04-30 | 2010-08-20 | Moet & Chandon | Procede de degorgement de vins mousseux |
AU2009256308A1 (en) * | 2008-06-03 | 2009-12-10 | Patrys Limited | Process for purification of antibodies |
EP2921501A1 (de) * | 2008-10-20 | 2015-09-23 | Abbvie Inc. | Isolation und Aufreinigung von Antikörpern mit Protein-A-Affinitätschromatografie |
CN104974251A (zh) | 2008-10-20 | 2015-10-14 | Abbvie公司 | 在抗体纯化过程中的病毒灭活 |
SI3318571T1 (sl) | 2011-03-16 | 2021-08-31 | F. Hoffmann-La Roche Ag | Ionska izmenjevalna kromatografija z izboljšano selektivnostjo za ločevanje polipeptidnih monomerov, agregatov in fragmentov s spremembo mobilne faze |
CN107915769A (zh) | 2011-03-29 | 2018-04-17 | 葛兰素史密斯克莱有限责任公司 | 用于蛋白纯化的缓冲液体系 |
CN103717284B (zh) * | 2011-06-08 | 2016-02-24 | 新加坡科技研究局 | 通过约束共水合色谱纯化生物制品 |
EP2682168A1 (de) | 2012-07-02 | 2014-01-08 | Millipore Corporation | Reinigung biologischer Moleküle |
US10023608B1 (en) | 2013-03-13 | 2018-07-17 | Amgen Inc. | Protein purification methods to remove impurities |
WO2015041218A1 (ja) * | 2013-09-17 | 2015-03-26 | 株式会社カネカ | 新規抗体精製方法及びそれから得られる抗体(Novel Antibody Purification Method and Antibody obtained therefrom)、並びに陽イオン交換基を用いた新規抗体精製法及びそれから得られる抗体(Novel Antibody Purification method using Cation Exchanger and Antibody obtained therefrom) |
CN109641050A (zh) | 2016-08-16 | 2019-04-16 | 里珍纳龙药品有限公司 | 用于对混合物中的个体抗体进行量化的方法 |
CN109923411B (zh) | 2016-10-25 | 2022-05-31 | 里珍纳龙药品有限公司 | 用于色谱数据分析的方法和系统 |
CN108152104B (zh) * | 2017-12-25 | 2020-08-11 | 上海微谱化工技术服务有限公司 | 超支化水性聚氨酯中二氧化硅填料的分离及定量方法 |
CN111902720A (zh) | 2018-03-21 | 2020-11-06 | 沃特世科技公司 | 基于非抗体高亲和力的样品制备、吸附剂、装置和方法 |
US11420916B2 (en) * | 2018-06-08 | 2022-08-23 | Board Of Regents, The University Of Texas System | Systems and methods for separation of olefins from mixtures that contain reducing agents |
TW202005694A (zh) | 2018-07-02 | 2020-02-01 | 美商里珍納龍藥品有限公司 | 自混合物製備多肽之系統及方法 |
WO2021064066A1 (en) * | 2019-10-04 | 2021-04-08 | Merck Patent Gmbh | Elution of monoclonal antibodies in protein a affinity chromatography |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3869436A (en) * | 1971-06-01 | 1975-03-04 | Statens Bakteriologiska Lab | Method for fractionating plasma proteins using peg and ion-exchangers |
DE3483620D1 (de) * | 1983-03-11 | 1991-01-03 | Fujirebio Kk | Verfahren zur bestimmung von liganden. |
US4683294A (en) * | 1985-04-03 | 1987-07-28 | Smith Kline Rit, S.A. | Process for the extraction and purification of proteins from culture media producing them |
ES2052602T3 (es) * | 1986-10-03 | 1994-07-16 | Ciba Geigy Ag | Nuevos peptidos afines a las linfocinas. |
US5151358A (en) * | 1989-06-13 | 1992-09-29 | Genencor International, Inc. | Processes for the recovery of naturally produced chymosin |
DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
US5443953A (en) * | 1993-12-08 | 1995-08-22 | Immunomedics, Inc. | Preparation and use of immunoconjugates |
US5561064A (en) * | 1994-02-01 | 1996-10-01 | Vical Incorporated | Production of pharmaceutical-grade plasmid DNA |
US5429746A (en) * | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
SE9503925D0 (sv) * | 1995-11-07 | 1995-11-07 | Pharmacia Biotech Ab | Separationsmedium för IgG |
WO1998017312A1 (fr) * | 1996-10-22 | 1998-04-30 | Chugai Seiyaku Kabushiki Kaisha | Remede contre les troubles septiques et comprenant un anticorps anti-il-8 en qualite d'ingredient actif |
AUPR110100A0 (en) * | 2000-10-30 | 2000-11-23 | Life Therapeutics Limited | Improved separation of macromolecules |
US7514078B2 (en) * | 2001-06-01 | 2009-04-07 | Cornell Research Foundation, Inc. | Methods of treating prostate cancer with anti-prostate specific membrane antigen antibodies |
US7998705B2 (en) * | 2002-08-06 | 2011-08-16 | FUJIFILM Diosynth Biotechnologies U.S.A., Inc | Increased dynamic binding capacity in ion exchange chromatography by addition of polyethylene glycol |
US20060275766A1 (en) * | 2003-01-07 | 2006-12-07 | Haurum John S | Method for manufacturing recombinant polyclonal proteins |
-
2004
- 2004-04-02 SE SE0400886A patent/SE0400886D0/xx unknown
-
2005
- 2005-03-31 EP EP05722292A patent/EP1729867B1/de active Active
- 2005-03-31 US US10/594,187 patent/US20070213513A1/en not_active Abandoned
- 2005-03-31 AT AT05722292T patent/ATE485880T1/de not_active IP Right Cessation
- 2005-03-31 WO PCT/SE2005/000467 patent/WO2005094960A1/en not_active Application Discontinuation
- 2005-03-31 DE DE602005024373T patent/DE602005024373D1/de active Active
- 2005-03-31 JP JP2007506111A patent/JP4831697B2/ja active Active
- 2005-03-31 CA CA002560012A patent/CA2560012A1/en not_active Abandoned
- 2005-03-31 AU AU2005228834A patent/AU2005228834A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US20070213513A1 (en) | 2007-09-13 |
EP1729867A1 (de) | 2006-12-13 |
CA2560012A1 (en) | 2005-10-13 |
JP2007530971A (ja) | 2007-11-01 |
WO2005094960A1 (en) | 2005-10-13 |
AU2005228834A1 (en) | 2005-10-13 |
EP1729867B1 (de) | 2010-10-27 |
ATE485880T1 (de) | 2010-11-15 |
JP4831697B2 (ja) | 2011-12-07 |
SE0400886D0 (sv) | 2004-04-02 |
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