WO2009003952A3 - Column and method for preparing a biological sample for protein profiling - Google Patents

Column and method for preparing a biological sample for protein profiling Download PDF

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Publication number
WO2009003952A3
WO2009003952A3 PCT/EP2008/058300 EP2008058300W WO2009003952A3 WO 2009003952 A3 WO2009003952 A3 WO 2009003952A3 EP 2008058300 W EP2008058300 W EP 2008058300W WO 2009003952 A3 WO2009003952 A3 WO 2009003952A3
Authority
WO
WIPO (PCT)
Prior art keywords
preparing
biological sample
relates
protein profiling
column
Prior art date
Application number
PCT/EP2008/058300
Other languages
French (fr)
Other versions
WO2009003952A2 (en
Inventor
Katleen Verleysen
Koen Sandra
Robin Tuytten
Patrick Sandra
Original Assignee
Pronota Nv
Katleen Verleysen
Koen Sandra
Robin Tuytten
Patrick Sandra
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pronota Nv, Katleen Verleysen, Koen Sandra, Robin Tuytten, Patrick Sandra filed Critical Pronota Nv
Publication of WO2009003952A2 publication Critical patent/WO2009003952A2/en
Publication of WO2009003952A3 publication Critical patent/WO2009003952A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a liquid chromatography column having a solid support comprising at least one immobilised crown ether. It also relates to a use of a crown ether for preparing a biological sample for protein profiling. The invention also relates to a method of preparing a biological sample for protein profiling, comprising the steps of: treating (2) the sample (1) with a cleavage reagent to generate peptides (7) comprising N-terminal primary amines (3, 4, 5), and sorting (9, 10) the peptides (7) by non-covalent interactions using a solid support (11, 13), wherein the non-covalent interactions are H-bridges or pi-pi (π-π) interactions. It also relates to a kit therefor.
PCT/EP2008/058300 2007-06-29 2008-06-27 Column and method for preparing a biological sample for protein profiling WO2009003952A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP07111409.4 2007-06-29
EP07111410.2 2007-06-29
EP07111410 2007-06-29
EP07111409 2007-06-29

Publications (2)

Publication Number Publication Date
WO2009003952A2 WO2009003952A2 (en) 2009-01-08
WO2009003952A3 true WO2009003952A3 (en) 2009-06-11

Family

ID=39967608

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/058300 WO2009003952A2 (en) 2007-06-29 2008-06-27 Column and method for preparing a biological sample for protein profiling

Country Status (1)

Country Link
WO (1) WO2009003952A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT106446B (en) * 2012-07-16 2014-09-18 Univ Da Beira Interior CHROMATOGRAPHIC MATRIX BASED ON POLYMER POLYMER AND GELANA METHOD FOR THEIR OBTAINATION
JP6217018B2 (en) * 2013-09-30 2017-10-25 大陽日酸株式会社 Protein or peptide analysis method and pyrylium peptide purification concentration kit
CN105297470B (en) * 2015-11-19 2017-07-04 李建勇 A kind of preparation method of three anti-intelligent dress ornaments
US20210148925A1 (en) * 2018-04-30 2021-05-20 Biotage Ab Qualitative analysis of proteins
JP6595086B1 (en) * 2018-12-26 2019-10-23 国立大学法人埼玉大学 Amino acid analysis method and amino acid analysis system
US20220339555A1 (en) * 2019-06-14 2022-10-27 Daicel Corporation Amine separation method using liquid chromatography
CN115245690A (en) * 2021-04-27 2022-10-28 中国科学院烟台海岸带研究所 System and method for enriching organic small molecular compounds in water body
CN114113287A (en) * 2022-01-25 2022-03-01 北京青莲百奥生物科技有限公司 Serum protein preparation method and serum proteome mass spectrum detection method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998040144A1 (en) * 1997-03-13 1998-09-17 Dionex Corporation Bifunctional crown ether-based cation-exchange stationary phase for liquid chromatography
WO2002077016A2 (en) * 2001-03-22 2002-10-03 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Methods and apparatus for gel-free qualitative and quantitative proteome analysis, and uses therefore

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998040144A1 (en) * 1997-03-13 1998-09-17 Dionex Corporation Bifunctional crown ether-based cation-exchange stationary phase for liquid chromatography
WO2002077016A2 (en) * 2001-03-22 2002-10-03 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Methods and apparatus for gel-free qualitative and quantitative proteome analysis, and uses therefore

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
GEVAERT KRIS ET AL: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides", NATURE BIOTECHNOLOGY MAY 2003,, vol. 21, no. 5, 1 May 2003 (2003-05-01), pages 566 - 569, XP002498621 *
GEVAERT KRIS ET AL: "Protein processing and other modifications analyzed by diagonal peptide chromatography", BIOCHIMICA ET BIOPHYSICA ACTA DEC 2006,, vol. 1764, no. 12, 1 December 2006 (2006-12-01), pages 1801 - 1810, XP002498620 *
KUHN K ET AL: "ISOLATION OF N-TERMINAL PROTEIN SEQUENCE TAGS FROM CYANOGEN BROMIDE CLEAVED PROTEINS AS A NOVEL APPROACH TO INVESTIGATE HYDROPHOBIC PROTEINS", JOURNAL OF PROTEOME RESEARCH, ACS, WASHINGTON, DC, US, vol. 2, 26 July 2003 (2003-07-26), pages 598 - 609, XP002328689, ISSN: 1535-3893 *
MCDONALD LUCY ET AL: "Positional proteomics: preparation of amino-terminal peptides as a strategy for proteome simplification and characterization.", NATURE PROTOCOLS, vol. 1, no. 4, 16 November 2006 (2006-11-16), pages 1790 - 1798, XP001538954, ISSN: 1750-2799 *
MCDONALD LUCY ET AL: "Positional proteomics: selective recovery and analysis of N-terminal proteolytic peptides.", NATURE METHODS DEC 2005, vol. 2, no. 12, December 2005 (2005-12-01), pages 955 - 957, XP009109028, ISSN: 1548-7091 *
SANDRA KOEN ET AL: "Combination of COFRADIC and high temperature-extended column length conventional liquid chromatography: a very efficient way to tackle complex protein samples, such as serum.", JOURNAL OF SEPARATION SCIENCE MAR 2007, vol. 30, no. 5, March 2007 (2007-03-01), pages 658 - 668, XP009108949, ISSN: 1615-9306 *
UDVARHELYI P M ET AL: "Direct resolution of some phenylglycines by liquid chromatography on a chiral crown ether phase.", CHIRALITY 1990, vol. 2, no. 3, 1990, pages 200 - 204, XP009108933, ISSN: 0899-0042 *
VERLEYSEN K ET AL: "Enantiomeric separation of some amino acids and derivatives by capillary electrophoresis with 18-crown-6-tetracarboxylic acid as chiral selector", JOURNAL OF MICROCOLUMN SEPARATIONS, PROVO, UT, US, vol. 11, no. 1, 1 January 1999 (1999-01-01), pages 37 - 43, XP002990906 *

Also Published As

Publication number Publication date
WO2009003952A2 (en) 2009-01-08

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