KR101656240B1 - 시료의 다중분석 - Google Patents

시료의 다중분석 Download PDF

Info

Publication number
KR101656240B1
KR101656240B1 KR1020107003485A KR20107003485A KR101656240B1 KR 101656240 B1 KR101656240 B1 KR 101656240B1 KR 1020107003485 A KR1020107003485 A KR 1020107003485A KR 20107003485 A KR20107003485 A KR 20107003485A KR 101656240 B1 KR101656240 B1 KR 101656240B1
Authority
KR
South Korea
Prior art keywords
tag
minutes
mixture
complex
delete delete
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020107003485A
Other languages
English (en)
Korean (ko)
Other versions
KR20100058485A (ko
Inventor
다니엘 제이 슈나이더
댄 뉴발런트
브루스 이튼
마르티 스탠튼
샤시 굽타
스테판 크레이머
도미닉 지흐
래리 골드
Original Assignee
소마로직, 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40260081&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR101656240(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 소마로직, 인크. filed Critical 소마로직, 인크.
Publication of KR20100058485A publication Critical patent/KR20100058485A/ko
Application granted granted Critical
Publication of KR101656240B1 publication Critical patent/KR101656240B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/334—Modified C
    • C12N2310/3341—5-Methylcytosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2541/00—Reactions characterised by directed evolution
    • C12Q2541/10—Reactions characterised by directed evolution the purpose being the selection or design of target specific nucleic acid binding sequences
    • C12Q2541/101—Selex

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Photometry And Measurement Of Optical Pulse Characteristics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
KR1020107003485A 2007-07-17 2008-07-17 시료의 다중분석 Active KR101656240B1 (ko)

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US95028307P 2007-07-17 2007-07-17
US95028107P 2007-07-17 2007-07-17
US95029307P 2007-07-17 2007-07-17
US60/950,293 2007-07-17
US60/950,281 2007-07-17
US60/950,283 2007-07-17
US3142008P 2008-02-26 2008-02-26
US61/031,420 2008-02-26
US5159408P 2008-05-08 2008-05-08
US61/051,594 2008-05-08

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020167024249A Division KR101747665B1 (ko) 2007-07-17 2008-07-17 시료의 다중분석

Publications (2)

Publication Number Publication Date
KR20100058485A KR20100058485A (ko) 2010-06-03
KR101656240B1 true KR101656240B1 (ko) 2016-09-09

Family

ID=40260081

Family Applications (3)

Application Number Title Priority Date Filing Date
KR1020107003485A Active KR101656240B1 (ko) 2007-07-17 2008-07-17 시료의 다중분석
KR1020167024249A Active KR101747665B1 (ko) 2007-07-17 2008-07-17 시료의 다중분석
KR1020107001155A Active KR101634608B1 (ko) 2007-07-17 2008-07-17 개선된 오프―레이트를 갖는 압타머를 생성하는 방법

Family Applications After (2)

Application Number Title Priority Date Filing Date
KR1020167024249A Active KR101747665B1 (ko) 2007-07-17 2008-07-17 시료의 다중분석
KR1020107001155A Active KR101634608B1 (ko) 2007-07-17 2008-07-17 개선된 오프―레이트를 갖는 압타머를 생성하는 방법

Country Status (17)

Country Link
US (1) US8409795B2 (cg-RX-API-DMAC7.html)
EP (10) EP2933340B1 (cg-RX-API-DMAC7.html)
JP (11) JP5684568B2 (cg-RX-API-DMAC7.html)
KR (3) KR101656240B1 (cg-RX-API-DMAC7.html)
CN (6) CN101809167B (cg-RX-API-DMAC7.html)
AU (3) AU2008275915B2 (cg-RX-API-DMAC7.html)
CA (4) CA3022666C (cg-RX-API-DMAC7.html)
DK (4) DK2489743T3 (cg-RX-API-DMAC7.html)
ES (6) ES2951068T3 (cg-RX-API-DMAC7.html)
FI (1) FI3284832T3 (cg-RX-API-DMAC7.html)
HR (1) HRP20150382T1 (cg-RX-API-DMAC7.html)
MX (7) MX2010000578A (cg-RX-API-DMAC7.html)
NO (1) NO2933340T3 (cg-RX-API-DMAC7.html)
PL (1) PL2489743T3 (cg-RX-API-DMAC7.html)
PT (2) PT2172566E (cg-RX-API-DMAC7.html)
SI (1) SI2489743T1 (cg-RX-API-DMAC7.html)
WO (3) WO2009012410A1 (cg-RX-API-DMAC7.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180119758A (ko) * 2017-04-26 2018-11-05 주식회사 압타머사이언스 백혈구에 선택적으로 결합하는 압타머 및 이의 용도

Families Citing this family (136)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5993279A (ja) * 1982-11-17 1984-05-29 富士通株式会社 ロボツトによるカ−ド，レシ−ト分離取出し方法
US20070166741A1 (en) 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US7964356B2 (en) * 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
US8975026B2 (en) 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US8404830B2 (en) * 2007-07-17 2013-03-26 Somalogic, Inc. Method for generating aptamers with improved off-rates
CA3022666C (en) 2007-07-17 2022-04-19 Somalogic, Inc. Multiplexed analyses of test samples
US8314052B2 (en) * 2009-03-23 2012-11-20 Base Pair Biotechnologies, Inc. Methods for simultaneous generation of functional ligands
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US9415392B2 (en) 2009-03-24 2016-08-16 The University Of Chicago Slip chip device and methods
US8569252B2 (en) * 2009-04-15 2013-10-29 Postech Academy-Industry Foundation Nucleolin specific aptamer and use thereof
AU2013203588C1 (en) * 2009-07-09 2016-06-30 Somalogic Operating Co., Inc. Method for generating aptamers with improved off-rates
JP5967687B2 (ja) * 2009-07-17 2016-08-10 Ｎｅｃソリューションイノベータ株式会社 スフィンゴシルホスホリルコリンに結合するアプタマー分子
US8236570B2 (en) 2009-11-03 2012-08-07 Infoscitex Methods for identifying nucleic acid ligands
US8841429B2 (en) * 2009-11-03 2014-09-23 Vivonics, Inc. Nucleic acid ligands against infectious prions
US9453845B2 (en) 2010-02-01 2016-09-27 Cell Signaling Technology, Inc. Mass spectroscopy analysis of mutant polypeptides in biological samples
EP2550000A4 (en) * 2010-03-24 2014-03-26 Advirna Inc RNAI COMPOUNDS OF REDUCED SIZE ADMINISTERING
SG184089A1 (en) * 2010-04-12 2012-11-29 Somalogic Inc APTAMERS TO ß-NGF AND THEIR USE IN TREATING ß-NGF MEDIATED DISEASES AND DISORDERS
AU2013202528B2 (en) * 2010-04-12 2015-07-30 Somalogic Operating Co., Inc. 5-position modified pyrimidines and their use
US20130116150A1 (en) * 2010-07-09 2013-05-09 Somalogic, Inc. Lung Cancer Biomarkers and Uses Thereof
ES2688048T3 (es) 2010-09-27 2018-10-30 Somalogic, Inc. Biomarcadores del mesotelioma y usos de los mismos
US9416360B2 (en) 2010-11-05 2016-08-16 MiRagen Therapeutics, Inc. Base modified oligonucleotides
KR101307616B1 (ko) * 2011-02-08 2013-09-12 경희대학교 산학협력단 Ｐｎａ 앱타머를 이용하여 금속을 분리하는 방법
WO2013099762A1 (ja) * 2011-12-28 2013-07-04 シスメックス株式会社 副腎皮質刺激ホルモンと結合する分子およびその利用
US9651556B2 (en) 2011-12-30 2017-05-16 Quest Diagnostics Investments Incorporated Aptamers and diagnostic methods for detecting the EGF receptor
JP6587934B2 (ja) 2012-03-28 2019-10-09 ソマロジック・インコーポレーテッド Ｐｄｇｆおよびｖｅｇｆ結合アプタマー、および、ｐｄｇｆおよびｖｅｇｆが介在する病気の治療へのそれらの使用
JP6200948B2 (ja) * 2012-05-25 2017-09-20 ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル マイクロ流体デバイス
AU2013271401B2 (en) * 2012-06-07 2018-04-05 Somalogic Operating Co., Inc. Aptamer-based multiplexed assays
CN102719430B (zh) * 2012-06-13 2013-06-12 湖南大学 一种检测组氨酸标签重组蛋白的核酸适配体分子信标探针及其检测方法
EP2868747B1 (en) * 2012-07-02 2018-04-04 UNIST (Ulsan National Institute of Science and Technology) Aptamer for periostin and anti-cancer composition including same
EP2690441A1 (en) 2012-07-26 2014-01-29 Rheinische Friedrich-Wilhelms-Universität Bonn Method of determining a protein in a sample
CN102912020B (zh) * 2012-10-20 2014-02-19 江南大学 一种测定赭曲霉毒素a的适配体传感器的构建方法
US10942184B2 (en) 2012-10-23 2021-03-09 Caris Science, Inc. Aptamers and uses thereof
CA2928520C (en) * 2012-10-23 2023-03-14 Caris Life Sciences Switzerland Holdings, S.A.R.L. Aptamers and uses thereof
WO2014074682A1 (en) 2012-11-07 2014-05-15 Somalogic, Inc. Chronic obstructive pulmonary disease (copd) biomarkers and uses thereof
US9939443B2 (en) 2012-12-19 2018-04-10 Caris Life Sciences Switzerland Holdings Gmbh Compositions and methods for aptamer screening
ES2710723T3 (es) 2013-03-14 2019-04-26 Somalogic Inc Aptámeros que se unen a IL-6 y su uso en el tratamiento o el diagnóstico de afecciones mediadas por IL-6
EP3660512B1 (en) 2013-03-15 2024-07-24 SomaLogic Operating Co., Inc. Nonalcoholic fatty liver disease (nafld) and nonalcoholic steatohepatitis (nash) biomarkers and uses thereof
WO2014144744A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Aptamer methods and compositions
JP6034261B2 (ja) * 2013-08-02 2016-11-30 日本電信電話株式会社 生体分子検出用チップの製造方法
JP6630273B2 (ja) 2013-09-09 2020-01-15 ソマロジック・インコーポレーテッド 安定性が向上したｐｄｇｆ及びｖｅｇｆアプタマー並びにｐｄｇｆ及びｖｅｇｆ媒介性の疾患及び障害の治療におけるそれらの使用
BR112016005957A2 (pt) 2013-09-24 2017-09-26 Somalogic Inc detecção de alvo multiaptâmero
EP3052653B1 (en) * 2013-10-02 2018-12-05 Tolle, Fabian A method of identifying or producing an aptamer
JP7008405B2 (ja) 2013-11-21 2022-01-25 ソマロジック・インコーポレーテッド シチジン－５－カルボキサミド修飾ヌクレオチド組成物及びそれに関連する方法
CN106029961B (zh) 2014-02-18 2022-07-26 私募蛋白质体运营有限公司 用于检测微生物的组合物和方法
WO2015164617A1 (en) 2014-04-24 2015-10-29 Somalogic, Inc. Tuberculosis biomarkers in urine and uses thereof
WO2015164616A1 (en) 2014-04-24 2015-10-29 Somalogic, Inc. Biomarkers for detection of tuberculosis
CN113176417B (zh) * 2014-07-01 2024-04-02 深圳迈瑞生物医疗电子股份有限公司 一种检测血液样本中血常规参数和c反应蛋白参数的方法
CA2961340C (en) 2014-09-26 2023-10-17 Somalogic, Inc. Cardiovascular risk event prediction and uses thereof
KR101849557B1 (ko) * 2014-09-29 2018-04-17 동국대학교 산학협력단 신규 세포 타겟 압타머 제조방법
US20170298343A1 (en) * 2014-10-02 2017-10-19 Rheinische Friedrich-Wilhelms Universität Bonn Method of identifying or producing an aptamer
KR101719285B1 (ko) 2014-11-04 2017-03-23 한국과학기술원 표적 물질에 의해 조절되는 핵산 중합효소 활성을 이용한 생체물질의 검출 및 정량 방법
JP6768651B2 (ja) 2014-11-24 2020-10-14 ソマロジック, インコーポレイテッドSomaLogic, Inc. 増殖分化因子１１と結合するための核酸化合物
CN104360077A (zh) * 2014-11-25 2015-02-18 重庆市科学技术研究院 一种用于检测多西环素残留的适配体核酸探针试剂盒、制备方法及其应用
WO2016123058A1 (en) 2015-01-27 2016-08-04 Somalogic, Inc. Biomarkers for detection of tuberculosis risk
US10370669B2 (en) 2015-02-09 2019-08-06 Somalogic, Inc. Nucleic acid compounds for binding growth differentiation factor 8
US10138486B2 (en) 2015-03-12 2018-11-27 The Regents Of The University Of Michigan Inhibitors of DEK protein and related methods
EP3295176A1 (en) 2015-05-08 2018-03-21 Somalogic, Inc. Biomarkers for detection of tuberculosis risk
CN107923918A (zh) 2015-09-09 2018-04-17 私募蛋白质体公司 用于开发个性化药物治疗计划和基于蛋白质组谱的靶向药物开发的方法
AU2016341208A1 (en) * 2015-10-20 2018-05-10 Sorrento Therapeutics, Inc. Intracellular delivery compounds
EP4513191A3 (en) 2016-02-08 2025-06-04 SomaLogic Operating Co., Inc. Nonalcoholic fatty liver disease (nafld) and nonalcoholic steatohepatitis (nash) biomarkers and uses thereof
GB201603789D0 (en) * 2016-03-04 2016-04-20 Apta Biosciences Ltd Oligonucleotides and methods for preparing
CN118910063A (zh) * 2016-07-01 2024-11-08 私募蛋白质体操作有限公司 包含修饰核苷的寡核苷酸
CN106248767B (zh) * 2016-07-15 2018-12-07 济南大学 一种用于检测癌细胞中h2s的三维纸分析器件的制备方法
CN107663220B (zh) * 2016-07-27 2020-10-02 上海伯豪医学检验所有限公司 修饰碱基、包含修饰碱基的核酸、适配体及其应用
CN109963937A (zh) * 2016-08-22 2019-07-02 日产化学株式会社 使用e-钙粘蛋白结合型核酸适配体的细胞支架材料
US11041849B2 (en) * 2016-12-01 2021-06-22 Aptitude Medical Systems, Inc. Methods and systems for identifying candidate nucleic acid agent
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
US11332736B2 (en) 2017-12-07 2022-05-17 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
US10481155B2 (en) * 2018-03-09 2019-11-19 Somalogic, Inc. Proteomic assay using quantum sensors
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
US11231420B2 (en) 2018-04-09 2022-01-25 Aptalogic, Inc. Selection and optimization of aptamers to recognize ebola markers
WO2019202448A1 (en) 2018-04-16 2019-10-24 University Of Cape Town A three-protein proteomic biomarker for prospective determination of risk for development of active tuberculosis
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
AT521238B1 (de) 2018-05-09 2020-02-15 Lifetaq Analytics Gmbh In-situ zellanalyse im zellkultursystem
CN112638845B (zh) * 2018-06-22 2024-07-30 私募蛋白质体运营有限公司 改进的蛋白质组学多重测定
WO2020037250A2 (en) * 2018-08-17 2020-02-20 The Trustees Of Columbia University In The City Of New York Stem-loop receptor-based field-effect transistor sensor devices target detection for small-molecule under physiological salt concentrations
EP3857224B1 (en) * 2018-09-25 2023-12-06 Siemens Healthcare Diagnostics Inc. Molecular trap for removing biotin interference from assays
WO2020068547A1 (en) 2018-09-25 2020-04-02 Siemens Healthcare Diagnostics Inc. Methods and compositions for removing biotin interference from assays using conjugated molecular traps
US12484814B2 (en) 2018-10-02 2025-12-02 WearOptimo Pty Ltd Actuator system
JP7548913B2 (ja) 2018-10-02 2024-09-10 ウェアオプティモ ピーティーワイ リミテッド 測定システム
WO2020077236A1 (en) 2018-10-12 2020-04-16 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
JP2022502069A (ja) 2018-10-15 2022-01-11 ソマロジック, インコーポレイテッドSomaLogic, Inc. 免疫グロブリンｇに結合する核酸化合物
US12402610B2 (en) 2018-11-09 2025-09-02 The Broad Institute, Inc. Methods and compositions for modulating innate lymphoid cell pathogenic effectors
EP3891299A1 (en) 2018-12-04 2021-10-13 F. Hoffmann-La Roche AG Spatially oriented quantum barcoding of cellular targets
US11739156B2 (en) 2019-01-06 2023-08-29 The Broad Institute, Inc. Massachusetts Institute of Technology Methods and compositions for overcoming immunosuppression
EP3942023A1 (en) 2019-03-18 2022-01-26 The Broad Institute, Inc. Compositions and methods for modulating metabolic regulators of t cell pathogenicity
SG11202111365QA (en) * 2019-05-17 2021-11-29 Somalogic Inc Controlling intersample analyte variability in complex biological matrices
WO2020243661A1 (en) 2019-05-31 2020-12-03 The Broad Institute, Inc. Methods for treating metabolic disorders by targeting adcy5
US11198908B2 (en) 2019-06-17 2021-12-14 Biorchestra Co., Ltd. Method for diagnosis of Alzheimer's disease using microRNA
KR102113078B1 (ko) * 2019-07-05 2020-05-20 광주과학기술원 압타머의 선별 방법
WO2021030627A1 (en) 2019-08-13 2021-02-18 The General Hospital Corporation Methods for predicting outcomes of checkpoint inhibition and treatment thereof
CN110396536A (zh) * 2019-08-14 2019-11-01 东南大学 一种基于分支滚环扩增的外泌体荧光检测传感器
US12421557B2 (en) 2019-08-16 2025-09-23 The Broad Institute, Inc. Methods for predicting outcomes and treating colorectal cancer using a cell atlas
WO2021046074A1 (en) 2019-09-03 2021-03-11 Somalogic, Inc. Cardiovascular risk event prediction and uses thereof
CN110618265B (zh) * 2019-09-12 2023-04-18 广东省药品检验所（广东省药品质量研究所、广东省口岸药品检验所） 一种沙丁胺醇的可视化检测方法
US11981922B2 (en) 2019-10-03 2024-05-14 Dana-Farber Cancer Institute, Inc. Methods and compositions for the modulation of cell interactions and signaling in the tumor microenvironment
US12195725B2 (en) 2019-10-03 2025-01-14 Dana-Farber Cancer Institute, Inc. Compositions and methods for modulating and detecting tissue specific TH17 cell pathogenicity
US11793787B2 (en) 2019-10-07 2023-10-24 The Broad Institute, Inc. Methods and compositions for enhancing anti-tumor immunity by targeting steroidogenesis
EP4045656A1 (en) 2019-10-16 2022-08-24 SomaLogic Operating Co., Inc. Nucleic acid compounds that bind to retinoic acid-inducible gene i protein
US20230093390A1 (en) * 2019-12-03 2023-03-23 Sung Chun Kim Method for obtaining profile of target molecule population of sample
IL294337A (en) * 2020-01-10 2022-08-01 Somalogic Operating Co Inc Methods for determining poor sugar loading
US12165747B2 (en) 2020-01-23 2024-12-10 The Broad Institute, Inc. Molecular spatial mapping of metastatic tumor microenvironment
JP7766605B2 (ja) 2020-02-10 2025-11-10 ソマロジック オペレーティング カンパニー インコーポレイテッド 非アルコール性脂肪性肝炎（ｎａｓｈ）のバイオマーカー及びその使用
CN111304202B (zh) * 2020-02-26 2023-03-28 西安交通大学 一种用于识别且增强hmgb1生物学活性的核酸适配子及其应用
AU2021261356A1 (en) * 2020-04-22 2022-12-15 Quantum-Si Incorporated Devices and methods for sequencing
CN113624724A (zh) * 2020-05-07 2021-11-09 廖世奇 一种适配体分子信标对靶分子的多元检测分析方法
AU2021275808A1 (en) * 2020-05-18 2022-11-10 Shanghai Polaris Biology Co., Ltd. Microbeads and uses thereof
CN111735869A (zh) * 2020-05-29 2020-10-02 中山大学 一种蛋白质的检测试剂及检测方法
CN116194138A (zh) 2020-08-07 2023-05-30 博德研究所 治疗性靶向癌症中经由xpr1:kidins220蛋白质复合物的磷酸盐调节异常
AU2021364805A1 (en) 2020-10-20 2023-05-18 Somalogic Operating Co., Inc. Cardiovascular event risk prediction
KR20230170688A (ko) 2021-04-13 2023-12-19 소마로직 오퍼레이팅 컴퍼니, 인코포레이티드 변형된 뉴클레오사이드
WO2022221496A1 (en) 2021-04-14 2022-10-20 Somalogic Operating Co., Inc. Nucleic acid compounds that bind coronavirus proteins
WO2022235975A2 (en) * 2021-05-06 2022-11-10 Systems Oncology, Llc Sirna constructs for inhibiting gene expression in targeted cancer cells
CA3221353A1 (en) 2021-06-15 2022-12-22 Laura SAMPSON Renal insufficiency prediction and uses thereof
US20240393337A1 (en) 2021-10-07 2024-11-28 Somalogic Operating Co., Inc. Lung Cancer Prediction and Uses Thereof
CN114058613B (zh) * 2021-11-18 2023-10-27 广州血液中心(中国医学科学院输血研究所广州分所、广州器官移植配型中心) 一种大体积、高灵敏度核酸提取方法
CN114295594B (zh) * 2021-12-06 2023-09-19 贵州理工学院 一种基于分子信标筛选三螺旋DNA嵌入剂的“turn on”型荧光传感器
JP2025505923A (ja) 2022-01-21 2025-03-05 ソマロジック オペレーティング カンパニー インコーポレイテッド 試料品質評価の方法
WO2023183873A1 (en) 2022-03-24 2023-09-28 Mensura Health Inc. Aptamers and uses thereof
AU2023260452A1 (en) 2022-04-24 2024-09-12 Somalogic Operating Co., Inc. Methods for sample quality assessment
US20250208119A1 (en) 2022-04-24 2025-06-26 Somalogic Operating Co., Inc. Methods for Sample Quality Assessment
KR20250002265A (ko) 2022-04-24 2025-01-07 소마로직 오퍼레이팅 컴퍼니, 인코포레이티드 샘플 품질 평가 방법
AU2023263246A1 (en) 2022-04-24 2024-09-19 Somalogic Operating Co., Inc. Methods for sample quality assessment
CA3259129A1 (en) 2022-07-14 2024-01-18 Somalogic Operating Co., Inc. Methods of assessing dementia risk
KR20250036127A (ko) 2022-07-14 2025-03-13 소마로직 오퍼레이팅 컴퍼니, 인코포레이티드 샘플 품질 평가 방법
JP2025534223A (ja) 2022-09-23 2025-10-15 ソマロジック オペレーティング カンパニー インコーポレイテッド タバコ使用状態を査定する方法
WO2024192141A1 (en) 2023-03-13 2024-09-19 Dana-Farber Cancer Institute, Inc. Treatment of cancers having a drug-resistant mesenchymal cell state
US20240424107A1 (en) 2023-04-07 2024-12-26 POSTECH Research and Business Development Foundation Preparation of site-specific polypeptide conjugates
WO2025049331A2 (en) * 2023-08-31 2025-03-06 Illumina, Inc. Aptamer detection techniques
WO2025129158A1 (en) 2023-12-15 2025-06-19 The Broad Institute, Inc. Engineered arc delivery vesicles and uses thereof
EP4603584A1 (en) * 2024-02-16 2025-08-20 Leica Microsystems CMS GmbH Affinity reagent, marker and method for analysing a biological sample
WO2025250808A1 (en) 2024-05-29 2025-12-04 The Brigham And Women’S Hospital, Inc. Anti-crispr delivery compositions and methods
CN119804610B (zh) * 2024-12-27 2025-11-07 南京师范大学 一种测定适配体和小分子靶标间相互作用的非变性质谱检测方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030219801A1 (en) * 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
US20060057573A1 (en) 2002-02-15 2006-03-16 Somalogic, Inc Methods and reagents for detecting target binding by nucleic acid ligands
US20060105341A1 (en) 2002-10-11 2006-05-18 Krause Henry M Trap-tagging: a novel method for the identification and purification of rna-protein complexes

Family Cites Families (95)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
DE229046T1 (de) 1985-03-30 1987-12-17 Marc Genf/Geneve Ballivet Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren.
US4753983A (en) 1986-05-07 1988-06-28 Bioprobe International, Inc. Polymeric matrix for affinity chromatography and immobilization of ligands
WO1989006694A1 (en) 1988-01-15 1989-07-27 Trustees Of The University Of Pennsylvania Process for selection of proteinaceous substances which mimic growth-inducing molecules
US5035996A (en) 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5874218A (en) 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Method for detecting a target compound in a substance using a nucleic acid ligand
US6346611B1 (en) 1990-06-11 2002-02-12 Gilead Sciences, Inc. High affinity TGfβ nucleic acid ligands and inhibitors
US5459015A (en) 1990-06-11 1995-10-17 Nexstar Pharmaceuticals, Inc. High-affinity RNA ligands of basic fibroblast growth factor
US5660985A (en) * 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US6001577A (en) 1998-06-08 1999-12-14 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US5763177A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US5861254A (en) * 1997-01-31 1999-01-19 Nexstar Pharmaceuticals, Inc. Flow cell SELEX
US5874557A (en) 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US6232071B1 (en) * 1990-06-11 2001-05-15 Gilead Sciences, Inc. Tenascin-C nucleic acid ligands
US5962219A (en) 1990-06-11 1999-10-05 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-selex
US5567588A (en) 1990-06-11 1996-10-22 University Research Corporation Systematic evolution of ligands by exponential enrichment: Solution SELEX
US5707796A (en) 1990-06-11 1998-01-13 Nexstar Pharmaceuticals, Inc. Method for selecting nucleic acids on the basis of structure
US6083696A (en) * 1990-06-11 2000-07-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands exponential enrichment: blended selex
US5705337A (en) 1990-06-11 1998-01-06 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-SELEX
US5763173A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5853984A (en) 1990-06-11 1998-12-29 Nexstar Pharmaceuticals, Inc. Use of nucleic acid ligands in flow cytometry
US5496938A (en) 1990-06-11 1996-03-05 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands to HIV-RT and HIV-1 rev
US6030776A (en) * 1990-06-11 2000-02-29 Nexstar Pharmaceuticals, Inc. Parallel SELEX
US5580737A (en) 1990-06-11 1996-12-03 Nexstar Pharmaceuticals, Inc. High-affinity nucleic acid ligands that discriminate between theophylline and caffeine
AU663053B2 (en) 1990-06-11 1995-09-28 Gilead Sciences, Inc. Nucleic acid ligands
US6716580B2 (en) 1990-06-11 2004-04-06 Somalogic, Inc. Method for the automated generation of nucleic acid ligands
US6168778B1 (en) 1990-06-11 2001-01-02 Nexstar Pharmaceuticals, Inc. Vascular endothelial growth factor (VEGF) Nucleic Acid Ligand Complexes
US5472841A (en) 1990-06-11 1995-12-05 Nexstar Pharmaceuticals, Inc. Methods for identifying nucleic acid ligands of human neutrophil elastase
US5693502A (en) 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US20040132067A1 (en) 1990-06-11 2004-07-08 Somalogic, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US5789163A (en) 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Enzyme linked oligonucleotide assays (ELONAS)
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
SE501013C2 (sv) 1990-10-01 1994-10-17 Pharmacia Biosensor Ab Förfarande för förbättring av analys med bindning till fast fas
CA2104698A1 (en) 1991-02-21 1992-08-22 John J. Toole Aptamers specific for biomolecules and methods of making
AU2580892A (en) 1991-09-05 1993-04-05 Isis Pharmaceuticals, Inc. Determination of oligonucleotides for therapeutics, diagnostics and research reagents
AU3222793A (en) * 1991-11-26 1993-06-28 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5312730A (en) * 1992-05-27 1994-05-17 Ciba Corning Diagnostics Corp. Immune complex transfer with lypophilic bridge
US5756291A (en) * 1992-08-21 1998-05-26 Gilead Sciences, Inc. Aptamers specific for biomolecules and methods of making
US5985548A (en) 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
US5580972A (en) 1993-06-14 1996-12-03 Nexstar Pharmaceuticals, Inc. Purine nucleoside modifications by palladium catalyzed methods
US5719273A (en) 1993-06-14 1998-02-17 Nexstar Pharmaceuticals, Inc. Palladium catalyzed nucleoside modifications methods using nucleophiles and carbon monoxide
US5428149A (en) * 1993-06-14 1995-06-27 Washington State University Research Foundation Method for palladium catalyzed carbon-carbon coulping and products
US5998142A (en) 1993-09-08 1999-12-07 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-SELEX
WO1995007364A1 (en) * 1993-09-08 1995-03-16 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands and improved methods for producing the same
CA2169535A1 (en) * 1993-09-17 1995-03-23 Larry Gold Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US6458539B1 (en) 1993-09-17 2002-10-01 Somalogic, Inc. Photoselection of nucleic acid ligands
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5610287A (en) 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
JP4899014B2 (ja) * 1995-06-02 2012-03-21 イーシー・テクノロジー・エルエルシー 求核試薬および一酸化炭素を用いるパラジウム触媒ヌクレオシド修飾方法
EP0833944B1 (en) 1995-06-07 2009-01-07 Gilead Sciences, Inc. Nucleic acid ligands that bind to and inhibit dna polymerases
US6183967B1 (en) * 1995-06-07 2001-02-06 Nexstar Pharmaceuticals Nucleic acid ligand inhibitors to DNA polymerases
US5737498A (en) 1995-07-11 1998-04-07 Beckman Instruments, Inc. Process automation method and apparatus
EP0864002A4 (en) * 1995-10-27 1999-07-28 Elliot R Ramberg METHODS AND COMPOSITIONS FOR DETECTION OF SPECIFIC NUCLEOTIDE SEQUENCES
US5727498A (en) * 1996-02-13 1998-03-17 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Retractable visual indicator assembly
US5945527A (en) 1996-05-30 1999-08-31 Nexstar Pharmaceuticals, Inc. Palladium catalyzed nucleoside modification methods using nucleophiles and carbon monoxide
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6051698A (en) 1997-06-06 2000-04-18 Janjic; Nebojsa Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
US6426335B1 (en) 1997-10-17 2002-07-30 Gilead Sciences, Inc. Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
US6235471B1 (en) 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
WO1999027133A1 (en) 1997-11-26 1999-06-03 Medical Research Council Improved selex procedure and an anti-cd4 aptamer
US6242246B1 (en) 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
US20070166741A1 (en) * 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
EP1049803A4 (en) 1997-12-15 2002-08-21 Nexstar Pharmaceuticals Inc HOMOGENEOUS DETECTION OF TARGET MOLECULES BY LIGAND NUCLEIC ACID AND LIGAND BEACON INTERACTION
US5989823A (en) 1998-09-18 1999-11-23 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
US6175001B1 (en) * 1998-10-16 2001-01-16 The Scripps Research Institute Functionalized pyrimidine nucleosides and nucleotides and DNA's incorporating same
US20030054360A1 (en) * 1999-01-19 2003-03-20 Larry Gold Method and apparatus for the automated generation of nucleic acid ligands
US6329145B1 (en) 1999-02-09 2001-12-11 Gilead Science, Inc. Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligand
US6376190B1 (en) 2000-09-22 2002-04-23 Somalogic, Inc. Modified SELEX processes without purified protein
AU2002224401B2 (en) * 2000-10-16 2007-12-06 Gilead Sciences, Inc. Nucleic acid ligands to the prostate specific membrane antigen
WO2002044726A1 (fr) * 2000-12-01 2002-06-06 International Reagents Corporation Procede de mesure ultrarapide et ultrasensible
WO2003014369A1 (en) 2001-08-09 2003-02-20 Somalogic, Inc. Nucleic acid ligands with intramolecular duplexes
EP1489171B1 (en) * 2002-03-19 2012-10-31 Fujitsu Limited Functional molecule and process for producing the same
US20030228603A1 (en) * 2002-04-05 2003-12-11 Cload Sharon T. Compositions selective for caffeine or aspartame and methods of using same
US7767803B2 (en) * 2002-06-18 2010-08-03 Archemix Corp. Stabilized aptamers to PSMA and their use as prostate cancer therapeutics
DE10241938A1 (de) 2002-09-10 2004-03-25 Noxxon Pharma Ag Verfahren zur Selektion von Nukleinsäureliganden
JP2004307464A (ja) * 2002-10-23 2004-11-04 Sankyo Co Ltd 糖部ｓ型配座の新規人工核酸
GB0226374D0 (en) * 2002-11-12 2002-12-18 Isis Innovation Ligands
WO2004063342A2 (en) * 2003-01-09 2004-07-29 Invitrogen Corporation Cellular delivery and activation polypeptide-nucleic acid complexes
WO2004085049A2 (en) * 2003-03-28 2004-10-07 The Regents Of The University Of California Preparation and application of encoded bead aggregates in combinatorial chemistry
US7672786B2 (en) 2003-07-02 2010-03-02 Sergey Krylov Non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM)—based methods for drug and diagnostic development
CA2535510C (en) * 2003-08-11 2013-10-15 Monogram Biosciences, Inc. Detecting and profiling molecular complexes
KR100540509B1 (ko) * 2004-01-07 2006-01-11 학교법인 포항공과대학교 수화젤레이터로 사용되는 2'-데옥시우리딘 유도체
EP1584923A3 (en) * 2004-04-07 2006-01-04 Roche Diagnostics GmbH Stabilization of biomolecules in samples
JP4839051B2 (ja) * 2004-10-08 2011-12-14 シスメックス株式会社 核酸プローブを用いる被検物質の検出方法
US20090105172A1 (en) * 2005-03-07 2009-04-23 Diener John L Stabilized Aptamers to PSMA and Their Use as Prostate Cancer Therapeutics
EP1901960B1 (en) * 2005-06-24 2010-05-05 Mag Aerospace Industries, Inc. Gray water interface valve systems and methods
EP1910837B1 (en) * 2005-07-01 2012-03-14 Arbor Vita Corporation Methods and compositions for diagnosis and treatment of influenza
ATE489482T1 (de) * 2005-08-19 2010-12-15 Nanosphere Inc Verfahren zur herstellung von hybridsubstraten mit dna und antikörpern sowie verwendungen davon
US20070166740A1 (en) * 2006-01-17 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
EP2207891B1 (en) * 2006-12-22 2012-07-25 Archemix LLC Materials and methods for the generation of transcripts comprising modified nucleotides
US20080189609A1 (en) * 2007-01-23 2008-08-07 Timothy Mark Larson Method and system for creating customized output
CA3022666C (en) * 2007-07-17 2022-04-19 Somalogic, Inc. Multiplexed analyses of test samples
JP2013220095A (ja) * 2012-04-12 2013-10-28 Toyohiro Fujita 苗床
JP2018078250A (ja) 2016-11-11 2018-05-17 株式会社ニューフレアテクノロジー マルチ荷電粒子ビーム描画装置

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060057573A1 (en) 2002-02-15 2006-03-16 Somalogic, Inc Methods and reagents for detecting target binding by nucleic acid ligands
US20030219801A1 (en) * 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
US20060105341A1 (en) 2002-10-11 2006-05-18 Krause Henry M Trap-tagging: a novel method for the identification and purification of rna-protein complexes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Anal. Chem. 2004, vol. 76, pp. 4066-4075.

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180119758A (ko) * 2017-04-26 2018-11-05 주식회사 압타머사이언스 백혈구에 선택적으로 결합하는 압타머 및 이의 용도
KR101993427B1 (ko) * 2017-04-26 2019-10-01 주식회사 압타머사이언스 백혈구에 선택적으로 결합하는 압타머 및 이의 용도

Also Published As

Publication number Publication date
US8409795B2 (en) 2013-04-02
JP2019039929A (ja) 2019-03-14
HRP20150382T1 (hr) 2015-05-08
JP2021129577A (ja) 2021-09-09
WO2009012420A1 (en) 2009-01-22
PT2489743E (pt) 2015-04-09
NO2933340T3 (cg-RX-API-DMAC7.html) 2018-02-03
JP2023116788A (ja) 2023-08-22
HK1133046A1 (en) 2010-03-12
DK3284832T5 (da) 2024-10-07
CN101802225B (zh) 2013-10-30
JP7755619B2 (ja) 2025-10-16
EP2626436A2 (en) 2013-08-14
AU2008275917B2 (en) 2014-08-21
EP2933340A1 (en) 2015-10-21
ES2604764T3 (es) 2017-03-09
JP2016122014A (ja) 2016-07-07
ES2533711T3 (es) 2015-04-14
JP6407195B2 (ja) 2018-10-17
ES2537322T5 (es) 2022-09-12
EP2489743B1 (en) 2015-01-07
AU2008276001A1 (en) 2009-01-22
AU2008275915B2 (en) 2013-07-11
MX2010000533A (es) 2010-04-30
DK3284832T3 (da) 2023-07-31
CA2693453C (en) 2018-12-11
MX344253B (es) 2016-12-09
CA2696431C (en) 2021-01-05
EP2069496A4 (en) 2010-01-27
EP2172566B1 (en) 2015-03-04
JP2015062416A (ja) 2015-04-09
PT2172566E (pt) 2015-06-23
AU2008275915A1 (en) 2009-01-22
JP5404620B2 (ja) 2014-02-05
EP2626436A3 (en) 2013-10-16
KR20100058485A (ko) 2010-06-03
MX2010000578A (es) 2010-04-30
DK2489743T3 (en) 2015-03-02
AU2008276001A2 (en) 2010-06-03
CA3022666C (en) 2022-04-19
ES2537322T3 (es) 2015-06-05
SI2489743T1 (sl) 2015-04-30
HK1141839A1 (en) 2010-11-19
EP2172566A1 (en) 2010-04-07
JP2016171810A (ja) 2016-09-29
ES3045958T3 (en) 2025-12-01
EP2076613B1 (en) 2016-09-07
EP2489743A2 (en) 2012-08-22
JP5684568B2 (ja) 2015-03-11
EP2172566B2 (en) 2022-05-18
MX375114B (es) 2025-03-06
CN118064441A (zh) 2024-05-24
AU2008275917A1 (en) 2009-01-22
CN103627792A (zh) 2014-03-12
AU2008275917A2 (en) 2010-04-29
EP4056711B1 (en) 2025-09-03
EP4056711A1 (en) 2022-09-14
JP7303244B2 (ja) 2023-07-04
HK1213020A1 (en) 2016-06-24
CN101809167A (zh) 2010-08-18
DK2172566T4 (da) 2022-06-13
JP5956404B2 (ja) 2016-07-27
EP2933340B1 (en) 2017-09-06
EP4056711C0 (en) 2025-09-03
EP3284832A3 (en) 2018-03-07
JP2020078311A (ja) 2020-05-28
MX355017B (es) 2018-04-02
EP3284832A2 (en) 2018-02-21
EP3284832B1 (en) 2023-06-07
KR101747665B1 (ko) 2017-06-15
HK1246368A1 (en) 2018-09-07
EP2076613A4 (en) 2012-11-28
EP2069529B1 (en) 2013-01-09
WO2009012418A2 (en) 2009-01-22
AU2008276001A9 (en) 2010-06-10
ES2647587T3 (es) 2017-12-22
EP2069496A1 (en) 2009-06-17
KR101634608B1 (ko) 2016-06-29
ES2951068T3 (es) 2023-10-17
DK2172566T3 (en) 2015-05-04
EP2076613A1 (en) 2009-07-08
KR20100044787A (ko) 2010-04-30
DK2069529T3 (da) 2013-04-15
CN104593373A (zh) 2015-05-06
JP6491610B2 (ja) 2019-03-27
JP2010533872A (ja) 2010-10-28
HK1132012A1 (zh) 2010-02-12
AU2008275915A2 (en) 2010-04-22
CA2693453A1 (en) 2009-01-22
WO2009012418A3 (en) 2009-03-05
JP2019004901A (ja) 2019-01-17
CA2696431A1 (en) 2009-01-22
KR20160108584A (ko) 2016-09-19
EP2069529A2 (en) 2009-06-17
EP2069529A4 (en) 2009-12-30
CN101802225A (zh) 2010-08-11
WO2009012410A1 (en) 2009-01-22
JP2014050398A (ja) 2014-03-20
JP2010533499A (ja) 2010-10-28
US20090098549A1 (en) 2009-04-16
FI3284832T3 (fi) 2023-08-01
PL2489743T3 (pl) 2015-06-30
CN107090457A (zh) 2017-08-25
MX341491B (es) 2016-08-23
CA2693448A1 (en) 2009-01-22
HK1174366A1 (en) 2013-06-07
EP2489743A3 (en) 2012-11-21
JP5938080B2 (ja) 2016-06-22
CA3022666A1 (en) 2009-01-22
MX2020009595A (es) 2020-10-07
EP2336314A1 (en) 2011-06-22
CN101809167B (zh) 2015-04-01

Similar Documents

Publication Publication Date Title
KR101656240B1 (ko) 시료의 다중분석
US7855054B2 (en) Multiplexed analyses of test samples
US9404919B2 (en) Multiplexed analyses of test samples
KR20150030655A (ko) 압타머-기반 다중 검정법
AU2017202493B2 (en) Multiplexed analyses of test samples
AU2013203360B2 (en) Multiplexed analyses of test samples
HK1236564A1 (en) Multiplexed analyses of test samples
HK1187380A (en) Method for detecting a target using chemically modified aptamers enriched for slow off-rates
HK1132012B (en) Analyses of test samples

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

R18-X000 Changes to party contact information recorded

St.27 status event code: A-3-3-R10-R18-oth-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

A201 Request for examination
E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

R15-X000 Change to inventor requested

St.27 status event code: A-3-3-R10-R15-oth-X000

R16-X000 Change to inventor recorded

St.27 status event code: A-3-3-R10-R16-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T12-X000 Administrative time limit extension not granted

St.27 status event code: U-3-3-T10-T12-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

R17-X000 Change to representative recorded

St.27 status event code: A-3-3-R10-R17-oth-X000

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

A107 Divisional application of patent
PA0104 Divisional application for international application

St.27 status event code: A-0-1-A10-A18-div-PA0104

St.27 status event code: A-0-1-A10-A16-div-PA0104

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 5

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 6

PN2301 Change of applicant

St.27 status event code: A-5-5-R10-R13-asn-PN2301

St.27 status event code: A-5-5-R10-R11-asn-PN2301

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 7

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 8

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 9

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 10

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-OTH-PR1001 (AS PROVIDED BY THE NATIONAL OFFICE)

Year of fee payment: 10