KR101634608B1 - 개선된 오프―레이트를 갖는 압타머를 생성하는 방법 - Google Patents

개선된 오프―레이트를 갖는 압타머를 생성하는 방법 Download PDF

Info

Publication number
KR101634608B1
KR101634608B1 KR1020107001155A KR20107001155A KR101634608B1 KR 101634608 B1 KR101634608 B1 KR 101634608B1 KR 1020107001155 A KR1020107001155 A KR 1020107001155A KR 20107001155 A KR20107001155 A KR 20107001155A KR 101634608 B1 KR101634608 B1 KR 101634608B1
Authority
KR
South Korea
Prior art keywords
delete delete
nucleic acid
target molecule
mixture
rate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020107001155A
Other languages
English (en)
Korean (ko)
Other versions
KR20100044787A (ko
Inventor
도미닉 지치
쉐리 케이. 윌콕스
크리스 보크
다니엘 제이. 쉬나이더
브루스 이톤
래리 골드
Original Assignee
소마로직, 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40260081&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR101634608(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 소마로직, 인크. filed Critical 소마로직, 인크.
Publication of KR20100044787A publication Critical patent/KR20100044787A/ko
Application granted granted Critical
Publication of KR101634608B1 publication Critical patent/KR101634608B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/334—Modified C
    • C12N2310/3341—5-Methylcytosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2541/00—Reactions characterised by directed evolution
    • C12Q2541/10—Reactions characterised by directed evolution the purpose being the selection or design of target specific nucleic acid binding sequences
    • C12Q2541/101—Selex

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Photometry And Measurement Of Optical Pulse Characteristics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
KR1020107001155A 2007-07-17 2008-07-17 개선된 오프―레이트를 갖는 압타머를 생성하는 방법 Active KR101634608B1 (ko)

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US95028307P 2007-07-17 2007-07-17
US95029307P 2007-07-17 2007-07-17
US95028107P 2007-07-17 2007-07-17
US60/950,283 2007-07-17
US60/950,281 2007-07-17
US60/950,293 2007-07-17
US3142008P 2008-02-26 2008-02-26
US61/031,420 2008-02-26
US5159408P 2008-05-08 2008-05-08
US61/051,594 2008-05-08

Publications (2)

Publication Number Publication Date
KR20100044787A KR20100044787A (ko) 2010-04-30
KR101634608B1 true KR101634608B1 (ko) 2016-06-29

Family

ID=40260081

Family Applications (3)

Application Number Title Priority Date Filing Date
KR1020107001155A Active KR101634608B1 (ko) 2007-07-17 2008-07-17 개선된 오프―레이트를 갖는 압타머를 생성하는 방법
KR1020167024249A Active KR101747665B1 (ko) 2007-07-17 2008-07-17 시료의 다중분석
KR1020107003485A Active KR101656240B1 (ko) 2007-07-17 2008-07-17 시료의 다중분석

Family Applications After (2)

Application Number Title Priority Date Filing Date
KR1020167024249A Active KR101747665B1 (ko) 2007-07-17 2008-07-17 시료의 다중분석
KR1020107003485A Active KR101656240B1 (ko) 2007-07-17 2008-07-17 시료의 다중분석

Country Status (17)

Country Link
US (1) US8409795B2 (cg-RX-API-DMAC7.html)
EP (10) EP2489743B1 (cg-RX-API-DMAC7.html)
JP (11) JP5404620B2 (cg-RX-API-DMAC7.html)
KR (3) KR101634608B1 (cg-RX-API-DMAC7.html)
CN (6) CN118064441A (cg-RX-API-DMAC7.html)
AU (3) AU2008275917B2 (cg-RX-API-DMAC7.html)
CA (4) CA3022666C (cg-RX-API-DMAC7.html)
DK (4) DK2172566T4 (cg-RX-API-DMAC7.html)
ES (6) ES2604764T3 (cg-RX-API-DMAC7.html)
FI (1) FI3284832T3 (cg-RX-API-DMAC7.html)
HR (1) HRP20150382T1 (cg-RX-API-DMAC7.html)
MX (7) MX2010000578A (cg-RX-API-DMAC7.html)
NO (1) NO2933340T3 (cg-RX-API-DMAC7.html)
PL (1) PL2489743T3 (cg-RX-API-DMAC7.html)
PT (2) PT2172566E (cg-RX-API-DMAC7.html)
SI (1) SI2489743T1 (cg-RX-API-DMAC7.html)
WO (3) WO2009012410A1 (cg-RX-API-DMAC7.html)

Families Citing this family (137)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5993279A (ja) * 1982-11-17 1984-05-29 富士通株式会社 ロボツトによるカ−ド，レシ−ト分離取出し方法
US20070166741A1 (en) 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US7964356B2 (en) * 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US8975026B2 (en) 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
ES2604764T3 (es) 2007-07-17 2017-03-09 Somalogic, Inc. Análisis de muestras de ensayo
US8404830B2 (en) * 2007-07-17 2013-03-26 Somalogic, Inc. Method for generating aptamers with improved off-rates
US8314052B2 (en) * 2009-03-23 2012-11-20 Base Pair Biotechnologies, Inc. Methods for simultaneous generation of functional ligands
CN104722342B (zh) 2009-03-24 2017-01-11 芝加哥大学 滑动式芯片装置和方法
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US8569252B2 (en) * 2009-04-15 2013-10-29 Postech Academy-Industry Foundation Nucleolin specific aptamer and use thereof
AU2013203588C1 (en) * 2009-07-09 2016-06-30 Somalogic Operating Co., Inc. Method for generating aptamers with improved off-rates
JP5967687B2 (ja) * 2009-07-17 2016-08-10 Ｎｅｃソリューションイノベータ株式会社 スフィンゴシルホスホリルコリンに結合するアプタマー分子
US8236570B2 (en) 2009-11-03 2012-08-07 Infoscitex Methods for identifying nucleic acid ligands
US8841429B2 (en) * 2009-11-03 2014-09-23 Vivonics, Inc. Nucleic acid ligands against infectious prions
US9453845B2 (en) 2010-02-01 2016-09-27 Cell Signaling Technology, Inc. Mass spectroscopy analysis of mutant polypeptides in biological samples
WO2011119852A1 (en) 2010-03-24 2011-09-29 Rxi Pharmaceuticals Corporation Reduced size self-delivering rnai compounds
EP2558478B1 (en) * 2010-04-12 2018-03-07 Somalogic, Inc. 5-position modified pyrimidines and their use
AU2013202528B2 (en) * 2010-04-12 2015-07-30 Somalogic Operating Co., Inc. 5-position modified pyrimidines and their use
SG186953A1 (en) * 2010-07-09 2013-02-28 Somalogic Inc Lung cancer biomarkers and uses thereof
CN103429753A (zh) 2010-09-27 2013-12-04 私募蛋白质体公司 间皮瘤生物标记及其用途
WO2012061810A1 (en) 2010-11-05 2012-05-10 Miragen Therapeutics Base modified oligonucleotides
KR101307616B1 (ko) * 2011-02-08 2013-09-12 경희대학교 산학협력단 Ｐｎａ 앱타머를 이용하여 금속을 분리하는 방법
WO2013099762A1 (ja) * 2011-12-28 2013-07-04 シスメックス株式会社 副腎皮質刺激ホルモンと結合する分子およびその利用
US9316647B2 (en) 2011-12-30 2016-04-19 Somalogic, Inc. Aptamers and diagnostic methods for detecting the EGF receptor
ES2742284T3 (es) 2012-03-28 2020-02-13 Somalogic Inc Aptámeros contra PDGF y VEGF y su utilización en el tratamiento de afecciones mediadas por PDGF y VEGF
EP2856177B1 (en) * 2012-05-25 2020-11-18 The University of North Carolina At Chapel Hill Microfluidic devices, solid supports for reagents and related methods
EP2859121B1 (en) * 2012-06-07 2016-11-30 Somalogic, Inc. Aptamer-based multiplexed assays
CN102719430B (zh) * 2012-06-13 2013-06-12 湖南大学 一种检测组氨酸标签重组蛋白的核酸适配体分子信标探针及其检测方法
EP2868747B1 (en) * 2012-07-02 2018-04-04 UNIST (Ulsan National Institute of Science and Technology) Aptamer for periostin and anti-cancer composition including same
EP2690441A1 (en) * 2012-07-26 2014-01-29 Rheinische Friedrich-Wilhelms-Universität Bonn Method of determining a protein in a sample
CN102912020B (zh) * 2012-10-20 2014-02-19 江南大学 一种测定赭曲霉毒素a的适配体传感器的构建方法
KR20150090072A (ko) 2012-10-23 2015-08-05 카리스 라이프 사이언스 스위스 홀딩스 게엠베하 압타머 및 이의 용도
US10942184B2 (en) 2012-10-23 2021-03-09 Caris Science, Inc. Aptamers and uses thereof
CN104812913B (zh) 2012-11-07 2019-03-15 私募蛋白质体公司 慢性阻塞性肺疾病(copd)生物标记及其用途
AU2013361323B2 (en) 2012-12-19 2018-09-06 Caris Science, Inc. Compositions and methods for aptamer screening
WO2014159669A2 (en) 2013-03-14 2014-10-02 Somalogic, Inc. Aptamers that bind to il-6 and their use in treating or diagnosing il-6 mediated conditions
DK3296747T3 (da) 2013-03-15 2020-03-30 Somalogic Inc Biomarkører for non-alkoholisk fedtleversygdom (NAFLD) og non-alkoholisk steatohepatitis (NASH) og anvendelser deraf
WO2014144744A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Aptamer methods and compositions
JP6034261B2 (ja) * 2013-08-02 2016-11-30 日本電信電話株式会社 生体分子検出用チップの製造方法
ES2834910T3 (es) 2013-09-09 2021-06-21 Somalogic Inc Aptámeros de PDGF y VEGF que tienen una estabilidad mejorada y su uso en el tratamiento de enfermedades y trastornos mediados por PDGF y VEGF
US9926566B2 (en) * 2013-09-24 2018-03-27 Somalogic, Inc. Multiaptamer target detection
EP3052653B1 (en) * 2013-10-02 2018-12-05 Tolle, Fabian A method of identifying or producing an aptamer
EP3071588A4 (en) 2013-11-21 2017-08-02 Somalogic, Inc. Cytidine-5-carboxamide modified nucleotide compositions and methods related thereto
CA3152415A1 (en) 2014-02-18 2015-08-27 Somalogic Operating Co., Inc. Compositions and methods for detecting microorganisms
WO2015164616A1 (en) 2014-04-24 2015-10-29 Somalogic, Inc. Biomarkers for detection of tuberculosis
WO2015164617A1 (en) 2014-04-24 2015-10-29 Somalogic, Inc. Tuberculosis biomarkers in urine and uses thereof
CN107656085B (zh) * 2014-07-01 2021-04-09 深圳迈瑞生物医疗电子股份有限公司 一种血液检测仪
US10670611B2 (en) 2014-09-26 2020-06-02 Somalogic, Inc. Cardiovascular risk event prediction and uses thereof
WO2016053015A1 (ko) * 2014-09-29 2016-04-07 동국대학교 산학협력단 신규 세포 타겟 압타머 제조방법
ES2768723T3 (es) * 2014-10-02 2020-06-23 Univ Bonn Rheinische Friedrich Wilhelms Un método para identificar o producir un aptámero
KR101719285B1 (ko) * 2014-11-04 2017-03-23 한국과학기술원 표적 물질에 의해 조절되는 핵산 중합효소 활성을 이용한 생체물질의 검출 및 정량 방법
CA2966925C (en) 2014-11-24 2023-08-29 Somalogic, Inc. Nucleic acid compounds for binding growth differentiation factor 11
CN104360077A (zh) * 2014-11-25 2015-02-18 重庆市科学技术研究院 一种用于检测多西环素残留的适配体核酸探针试剂盒、制备方法及其应用
WO2016123058A1 (en) 2015-01-27 2016-08-04 Somalogic, Inc. Biomarkers for detection of tuberculosis risk
TWI708845B (zh) 2015-02-09 2020-11-01 美商身體邏輯股份有限公司 用於結合生長分化因數8之核酸化合物
US10138486B2 (en) 2015-03-12 2018-11-27 The Regents Of The University Of Michigan Inhibitors of DEK protein and related methods
EP3295176A1 (en) 2015-05-08 2018-03-21 Somalogic, Inc. Biomarkers for detection of tuberculosis risk
EP3347720A1 (en) 2015-09-09 2018-07-18 Somalogic, Inc. Methods for developing personalized drug treatment plans and targeted drug development based on proteomic profiles
EP3365375A4 (en) * 2015-10-20 2019-09-04 Sorrento Therapeutics, Inc. COMPOUNDS FOR INTRA-CELLULAR ADMINISTRATION
KR102687249B1 (ko) 2016-02-08 2024-07-22 소마로직 오퍼레이팅 컴퍼니, 인코포레이티드 비알코올성 지방간 질환 (nafld)과 비알코올성 지방간염 (nash) 생물마커 및 이들의 용도
GB201603789D0 (en) * 2016-03-04 2016-04-20 Apta Biosciences Ltd Oligonucleotides and methods for preparing
CN109312346B (zh) * 2016-07-01 2024-07-30 私募蛋白质体操作有限公司 包含修饰核苷的寡核苷酸
CN106248767B (zh) * 2016-07-15 2018-12-07 济南大学 一种用于检测癌细胞中h2s的三维纸分析器件的制备方法
CN107663220B (zh) * 2016-07-27 2020-10-02 上海伯豪医学检验所有限公司 修饰碱基、包含修饰碱基的核酸、适配体及其应用
WO2018038076A1 (ja) * 2016-08-22 2018-03-01 日産化学工業株式会社 Ｅ－カドヘリン結合型核酸アプタマーを用いる細胞足場材料
US11041849B2 (en) * 2016-12-01 2021-06-22 Aptitude Medical Systems, Inc. Methods and systems for identifying candidate nucleic acid agent
KR101993427B1 (ko) * 2017-04-26 2019-10-01 주식회사 압타머사이언스 백혈구에 선택적으로 결합하는 압타머 및 이의 용도
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
WO2019113506A1 (en) 2017-12-07 2019-06-13 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
US10481155B2 (en) * 2018-03-09 2019-11-19 Somalogic, Inc. Proteomic assay using quantum sensors
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
US11231420B2 (en) 2018-04-09 2022-01-25 Aptalogic, Inc. Selection and optimization of aptamers to recognize ebola markers
US20210140977A1 (en) 2018-04-16 2021-05-13 University Of Cape Town A three-protein proteomic biomarker for prospective determination of risk for development of active tuberculosis
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
AT521238B1 (de) 2018-05-09 2020-02-15 Lifetaq Analytics Gmbh In-situ zellanalyse im zellkultursystem
CN112638845B (zh) * 2018-06-22 2024-07-30 私募蛋白质体运营有限公司 改进的蛋白质组学多重测定
WO2020037250A2 (en) * 2018-08-17 2020-02-20 The Trustees Of Columbia University In The City Of New York Stem-loop receptor-based field-effect transistor sensor devices target detection for small-molecule under physiological salt concentrations
EP4325221B1 (en) * 2018-09-25 2025-10-29 Siemens Healthcare Diagnostics Inc. Molecular trap for removing fluorescein interference from assays
WO2020068547A1 (en) 2018-09-25 2020-04-02 Siemens Healthcare Diagnostics Inc. Methods and compositions for removing biotin interference from assays using conjugated molecular traps
US12048558B2 (en) 2018-10-02 2024-07-30 WearOptimo Pty Ltd System for determining fluid level in a biological subject
WO2020069569A1 (en) 2018-10-02 2020-04-09 WearOptimo Pty Ltd Actuator system
US20220411783A1 (en) 2018-10-12 2022-12-29 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
JP2022502069A (ja) 2018-10-15 2022-01-11 ソマロジック, インコーポレイテッドSomaLogic, Inc. 免疫グロブリンｇに結合する核酸化合物
US12402610B2 (en) 2018-11-09 2025-09-02 The Broad Institute, Inc. Methods and compositions for modulating innate lymphoid cell pathogenic effectors
EP3891299A1 (en) 2018-12-04 2021-10-13 F. Hoffmann-La Roche AG Spatially oriented quantum barcoding of cellular targets
US11739156B2 (en) 2019-01-06 2023-08-29 The Broad Institute, Inc. Massachusetts Institute of Technology Methods and compositions for overcoming immunosuppression
US20220142948A1 (en) 2019-03-18 2022-05-12 The Broad Institute, Inc. Compositions and methods for modulating metabolic regulators of t cell pathogenicity
CA3140053A1 (en) * 2019-05-17 2020-11-26 Somalogic, Inc. Controlling intersample analyte variability in complex biological matrices
WO2020243661A1 (en) 2019-05-31 2020-12-03 The Broad Institute, Inc. Methods for treating metabolic disorders by targeting adcy5
US11198908B2 (en) 2019-06-17 2021-12-14 Biorchestra Co., Ltd. Method for diagnosis of Alzheimer's disease using microRNA
KR102113078B1 (ko) * 2019-07-05 2020-05-20 광주과학기술원 압타머의 선별 방법
WO2021030627A1 (en) 2019-08-13 2021-02-18 The General Hospital Corporation Methods for predicting outcomes of checkpoint inhibition and treatment thereof
CN110396536A (zh) * 2019-08-14 2019-11-01 东南大学 一种基于分支滚环扩增的外泌体荧光检测传感器
US12421557B2 (en) 2019-08-16 2025-09-23 The Broad Institute, Inc. Methods for predicting outcomes and treating colorectal cancer using a cell atlas
MX2022002314A (es) 2019-09-03 2022-06-02 Somalogic Operating Co Inc Prediccion del riesgo de eventos cardiovasculares y sus usos.
CN110618265B (zh) * 2019-09-12 2023-04-18 广东省药品检验所（广东省药品质量研究所、广东省口岸药品检验所） 一种沙丁胺醇的可视化检测方法
US11981922B2 (en) 2019-10-03 2024-05-14 Dana-Farber Cancer Institute, Inc. Methods and compositions for the modulation of cell interactions and signaling in the tumor microenvironment
US12195725B2 (en) 2019-10-03 2025-01-14 Dana-Farber Cancer Institute, Inc. Compositions and methods for modulating and detecting tissue specific TH17 cell pathogenicity
US11793787B2 (en) 2019-10-07 2023-10-24 The Broad Institute, Inc. Methods and compositions for enhancing anti-tumor immunity by targeting steroidogenesis
JP2022552192A (ja) 2019-10-16 2022-12-15 ソマロジック オペレーティング カンパニー インコーポレイテッド レチノイン酸誘導遺伝子ｉタンパク質に結合する核酸化合物
US20230093390A1 (en) * 2019-12-03 2023-03-23 Sung Chun Kim Method for obtaining profile of target molecule population of sample
JP7792338B2 (ja) * 2020-01-10 2025-12-25 ソマロジック オペレーティング カンパニー インコーポレイテッド 耐糖能異常障害を決定する方法
US12165747B2 (en) 2020-01-23 2024-12-10 The Broad Institute, Inc. Molecular spatial mapping of metastatic tumor microenvironment
CN115066616A (zh) 2020-02-10 2022-09-16 私募蛋白质体操作有限公司 非酒精性脂肪性肝炎(nash)生物标志物及其用途
CN111304202B (zh) * 2020-02-26 2023-03-28 西安交通大学 一种用于识别且增强hmgb1生物学活性的核酸适配子及其应用
KR20220159496A (ko) * 2020-04-22 2022-12-02 퀀텀-에스아이 인코포레이티드 시퀀싱을 위한 장치 및 방법
CN113624724A (zh) * 2020-05-07 2021-11-09 廖世奇 一种适配体分子信标对靶分子的多元检测分析方法
WO2021233303A1 (en) * 2020-05-18 2021-11-25 Shanghai Polaris Biology Co., Ltd. Microbeads and uses thereof
CN111735869A (zh) * 2020-05-29 2020-10-02 中山大学 一种蛋白质的检测试剂及检测方法
CN116194138A (zh) 2020-08-07 2023-05-30 博德研究所 治疗性靶向癌症中经由xpr1:kidins220蛋白质复合物的磷酸盐调节异常
EP4614156A2 (en) 2020-10-20 2025-09-10 SomaLogic Operating Co., Inc. Cardiovascular event risk prediction
WO2022221241A1 (en) 2021-04-13 2022-10-20 Somalogic Operating Co., Inc. Modified nucleosides
WO2022221496A1 (en) 2021-04-14 2022-10-20 Somalogic Operating Co., Inc. Nucleic acid compounds that bind coronavirus proteins
US20240240178A1 (en) * 2021-05-06 2024-07-18 Systems Oncology, Llc Sirna constructs for inhibiting gene expression in targeted cancer cells
CA3221353A1 (en) 2021-06-15 2022-12-22 Laura SAMPSON Renal insufficiency prediction and uses thereof
JP2024540836A (ja) 2021-10-07 2024-11-06 ソマロジック オペレーティング カンパニー インコーポレイテッド 肺癌の予測及びその利用
CN114058613B (zh) * 2021-11-18 2023-10-27 广州血液中心(中国医学科学院输血研究所广州分所、广州器官移植配型中心) 一种大体积、高灵敏度核酸提取方法
CN114295594B (zh) * 2021-12-06 2023-09-19 贵州理工学院 一种基于分子信标筛选三螺旋DNA嵌入剂的“turn on”型荧光传感器
MX2024008874A (es) 2022-01-21 2024-07-29 Somalogic Operating Co Inc Metodos de evaluacion de calidad de muestras.
WO2023183873A1 (en) 2022-03-24 2023-09-28 Mensura Health Inc. Aptamers and uses thereof
KR20250002265A (ko) 2022-04-24 2025-01-07 소마로직 오퍼레이팅 컴퍼니, 인코포레이티드 샘플 품질 평가 방법
JP2025516098A (ja) 2022-04-24 2025-05-27 ソマロジック オペレーティング カンパニー インコーポレイテッド 試料品質評価の方法
EP4515249A1 (en) 2022-04-24 2025-03-05 SomaLogic Operating Co., Inc. Methods for sample quality assessment
CA3246311A1 (en) 2022-04-24 2023-11-02 Somalogic Operating Co., Inc. METHODS FOR EVALUATING THE QUALITY OF A SAMPLE
WO2024015485A1 (en) 2022-07-14 2024-01-18 Somalogic Operating Co., Inc. Methods of assessing dementia risk
KR20250036127A (ko) 2022-07-14 2025-03-13 소마로직 오퍼레이팅 컴퍼니, 인코포레이티드 샘플 품질 평가 방법
WO2024064322A2 (en) 2022-09-23 2024-03-28 Somalogic Operating Co., Inc. Methods of assessing tobacco use status
WO2024192141A1 (en) 2023-03-13 2024-09-19 Dana-Farber Cancer Institute, Inc. Treatment of cancers having a drug-resistant mesenchymal cell state
EP4442282A1 (en) 2023-04-07 2024-10-09 POSTECH Research and Business Development Foundation Preparation of site-specific polypeptide conjugates
WO2025049331A2 (en) * 2023-08-31 2025-03-06 Illumina, Inc. Aptamer detection techniques
WO2025129158A1 (en) 2023-12-15 2025-06-19 The Broad Institute, Inc. Engineered arc delivery vesicles and uses thereof
EP4603584A1 (en) * 2024-02-16 2025-08-20 Leica Microsystems CMS GmbH Affinity reagent, marker and method for analysing a biological sample
WO2025250808A1 (en) 2024-05-29 2025-12-04 The Brigham And Women’S Hospital, Inc. Anti-crispr delivery compositions and methods
CN119804610B (zh) * 2024-12-27 2025-11-07 南京师范大学 一种测定适配体和小分子靶标间相互作用的非变性质谱检测方法

Family Cites Families (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
DE3546806C2 (cg-RX-API-DMAC7.html) 1985-03-30 1991-03-28 Marc Genf/Geneve Ch Ballivet
US4753983A (en) 1986-05-07 1988-06-28 Bioprobe International, Inc. Polymeric matrix for affinity chromatography and immobilization of ligands
WO1989006694A1 (en) 1988-01-15 1989-07-27 Trustees Of The University Of Pennsylvania Process for selection of proteinaceous substances which mimic growth-inducing molecules
US5035996A (en) 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5763177A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US5660985A (en) 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US5874557A (en) 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5861254A (en) * 1997-01-31 1999-01-19 Nexstar Pharmaceuticals, Inc. Flow cell SELEX
US6346611B1 (en) * 1990-06-11 2002-02-12 Gilead Sciences, Inc. High affinity TGfβ nucleic acid ligands and inhibitors
US5874218A (en) 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Method for detecting a target compound in a substance using a nucleic acid ligand
US6030776A (en) * 1990-06-11 2000-02-29 Nexstar Pharmaceuticals, Inc. Parallel SELEX
US5496938A (en) 1990-06-11 1996-03-05 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands to HIV-RT and HIV-1 rev
US5962219A (en) 1990-06-11 1999-10-05 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-selex
US20040132067A1 (en) 1990-06-11 2004-07-08 Somalogic, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US5789163A (en) 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Enzyme linked oligonucleotide assays (ELONAS)
US5705337A (en) 1990-06-11 1998-01-06 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-SELEX
US5472841A (en) 1990-06-11 1995-12-05 Nexstar Pharmaceuticals, Inc. Methods for identifying nucleic acid ligands of human neutrophil elastase
US5707796A (en) 1990-06-11 1998-01-13 Nexstar Pharmaceuticals, Inc. Method for selecting nucleic acids on the basis of structure
DE69128350T2 (de) 1990-06-11 1998-03-26 Nexstar Pharmaceuticals Inc Nukleinsäureliganden
US6001577A (en) 1998-06-08 1999-12-14 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US5763173A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US6232071B1 (en) * 1990-06-11 2001-05-15 Gilead Sciences, Inc. Tenascin-C nucleic acid ligands
US6083696A (en) * 1990-06-11 2000-07-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands exponential enrichment: blended selex
US6168778B1 (en) 1990-06-11 2001-01-02 Nexstar Pharmaceuticals, Inc. Vascular endothelial growth factor (VEGF) Nucleic Acid Ligand Complexes
US5580737A (en) 1990-06-11 1996-12-03 Nexstar Pharmaceuticals, Inc. High-affinity nucleic acid ligands that discriminate between theophylline and caffeine
US6716580B2 (en) * 1990-06-11 2004-04-06 Somalogic, Inc. Method for the automated generation of nucleic acid ligands
US5459015A (en) 1990-06-11 1995-10-17 Nexstar Pharmaceuticals, Inc. High-affinity RNA ligands of basic fibroblast growth factor
US5567588A (en) 1990-06-11 1996-10-22 University Research Corporation Systematic evolution of ligands by exponential enrichment: Solution SELEX
US5693502A (en) 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5853984A (en) 1990-06-11 1998-12-29 Nexstar Pharmaceuticals, Inc. Use of nucleic acid ligands in flow cytometry
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
SE501013C2 (sv) 1990-10-01 1994-10-17 Pharmacia Biosensor Ab Förfarande för förbättring av analys med bindning till fast fas
AU1435492A (en) 1991-02-21 1992-09-15 Gilead Sciences, Inc. Aptamer specific for biomolecules and method of making
WO1993005182A1 (en) 1991-09-05 1993-03-18 Isis Pharmaceuticals, Inc. Determination of oligonucleotides for therapeutics, diagnostics and research reagents
EP0637965B1 (en) * 1991-11-26 2002-10-16 Isis Pharmaceuticals, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5312730A (en) * 1992-05-27 1994-05-17 Ciba Corning Diagnostics Corp. Immune complex transfer with lypophilic bridge
US5756291A (en) * 1992-08-21 1998-05-26 Gilead Sciences, Inc. Aptamers specific for biomolecules and methods of making
US5985548A (en) 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
US5428149A (en) * 1993-06-14 1995-06-27 Washington State University Research Foundation Method for palladium catalyzed carbon-carbon coulping and products
US5719273A (en) 1993-06-14 1998-02-17 Nexstar Pharmaceuticals, Inc. Palladium catalyzed nucleoside modifications methods using nucleophiles and carbon monoxide
US5580972A (en) 1993-06-14 1996-12-03 Nexstar Pharmaceuticals, Inc. Purine nucleoside modifications by palladium catalyzed methods
EP0724647A4 (en) * 1993-09-08 2003-09-17 Gilead Sciences Inc NUCLEIC ACIDS AS LIGANDS AND IMPROVED PRODUCTION METHODS
US5998142A (en) 1993-09-08 1999-12-07 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-SELEX
US6458539B1 (en) 1993-09-17 2002-10-01 Somalogic, Inc. Photoselection of nucleic acid ligands
JP3966478B2 (ja) 1993-09-17 2007-08-29 ソマロジック、インコーポレイテッド 指数関数的富化によるリガンドの系統的進化：核酸リガンドの光選択と溶液ｓｅｌｅｘ
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5610287A (en) 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
JP4899014B2 (ja) * 1995-06-02 2012-03-21 イーシー・テクノロジー・エルエルシー 求核試薬および一酸化炭素を用いるパラジウム触媒ヌクレオシド修飾方法
ATE420202T1 (de) 1995-06-07 2009-01-15 Gilead Sciences Inc Nukleinsäure liganden die dna-polymerase binden und inhibieren
US6183967B1 (en) 1995-06-07 2001-02-06 Nexstar Pharmaceuticals Nucleic acid ligand inhibitors to DNA polymerases
US5737498A (en) 1995-07-11 1998-04-07 Beckman Instruments, Inc. Process automation method and apparatus
EP0864002A4 (en) * 1995-10-27 1999-07-28 Elliot R Ramberg METHODS AND COMPOSITIONS FOR DETECTION OF SPECIFIC NUCLEOTIDE SEQUENCES
US5727498A (en) * 1996-02-13 1998-03-17 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Retractable visual indicator assembly
US5945527A (en) * 1996-05-30 1999-08-31 Nexstar Pharmaceuticals, Inc. Palladium catalyzed nucleoside modification methods using nucleophiles and carbon monoxide
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6426335B1 (en) 1997-10-17 2002-07-30 Gilead Sciences, Inc. Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
US6051698A (en) 1997-06-06 2000-04-18 Janjic; Nebojsa Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
US6235471B1 (en) 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
AU1252099A (en) 1997-11-26 1999-06-15 Medical Research Council Improved selex procedure and an anti-cd4 aptamer
AU3909199A (en) 1997-12-15 1999-07-05 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
US6242246B1 (en) * 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
US5989823A (en) 1998-09-18 1999-11-23 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
US20070166741A1 (en) * 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US6175001B1 (en) * 1998-10-16 2001-01-16 The Scripps Research Institute Functionalized pyrimidine nucleosides and nucleotides and DNA's incorporating same
US20030054360A1 (en) * 1999-01-19 2003-03-20 Larry Gold Method and apparatus for the automated generation of nucleic acid ligands
US6329145B1 (en) 1999-02-09 2001-12-11 Gilead Science, Inc. Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligand
US6376190B1 (en) 2000-09-22 2002-04-23 Somalogic, Inc. Modified SELEX processes without purified protein
AU2440102A (en) * 2000-10-16 2002-04-29 Gilead Sciences Inc Nucleic acid ligands to the prostate specific membrane antigen
AU2002224128A1 (en) * 2000-12-01 2002-06-11 International Reagents Corporation Method for ultra-rapid and ultra-sensitive measurement
WO2003014369A1 (en) 2001-08-09 2003-02-20 Somalogic, Inc. Nucleic acid ligands with intramolecular duplexes
EP1481090A4 (en) * 2002-02-15 2006-08-09 Somalogic Inc METHOD AND REAGENTS FOR DETECTING BINDING OF TARGET MOLECULES BY NUCLEIC ACID ELECTRODES
US20030219801A1 (en) * 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
EP1489171B1 (en) * 2002-03-19 2012-10-31 Fujitsu Limited Functional molecule and process for producing the same
US20030228603A1 (en) * 2002-04-05 2003-12-11 Cload Sharon T. Compositions selective for caffeine or aspartame and methods of using same
US7767803B2 (en) * 2002-06-18 2010-08-03 Archemix Corp. Stabilized aptamers to PSMA and their use as prostate cancer therapeutics
DE10241938A1 (de) * 2002-09-10 2004-03-25 Noxxon Pharma Ag Verfahren zur Selektion von Nukleinsäureliganden
CA2407825A1 (en) * 2002-10-11 2004-04-11 Andrew J. Simmonds Trap-tagging: a novel method for the identification and purification of rna-protein complexes
JP2004307464A (ja) * 2002-10-23 2004-11-04 Sankyo Co Ltd 糖部ｓ型配座の新規人工核酸
GB0226374D0 (en) * 2002-11-12 2002-12-18 Isis Innovation Ligands
JP2006517790A (ja) * 2003-01-09 2006-08-03 インヴィトロジェン コーポレーション ポリペプチド−核酸複合体の細胞の送達および活性化
US20040235053A1 (en) * 2003-03-28 2004-11-25 The Regents Of The University Of California Preparation and application of encoded bead aggregates in combinatorial chemistry
US7672786B2 (en) 2003-07-02 2010-03-02 Sergey Krylov Non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM)—based methods for drug and diagnostic development
AU2004267420A1 (en) * 2003-08-11 2005-03-03 Monogram Biosciences, Inc. Detecting and profiling molecular complexes
KR100540509B1 (ko) * 2004-01-07 2006-01-11 학교법인 포항공과대학교 수화젤레이터로 사용되는 2'-데옥시우리딘 유도체
EP1584923A3 (en) * 2004-04-07 2006-01-04 Roche Diagnostics GmbH Stabilization of biomolecules in samples
JP4839051B2 (ja) * 2004-10-08 2011-12-14 シスメックス株式会社 核酸プローブを用いる被検物質の検出方法
WO2006096754A2 (en) * 2005-03-07 2006-09-14 Archemix Corp. Stabilized aptamers to psma and their use as prostate cancer therapeutics
JP4368412B2 (ja) * 2005-06-24 2009-11-18 マグ エアロスペース インダストリーズ インコーポレイテッド 軽度汚水インターフェイスバルブ装置および方法
AU2006276813A1 (en) * 2005-07-01 2007-02-15 Arbor Vita Corporation Methods and compositions for diagnosis and treatment of influenza
WO2007024676A2 (en) * 2005-08-19 2007-03-01 Nanosphere, Inc. Methods for preparing hybrid substrates comprising dna and antibodies and uses thereof
CN101374965B (zh) * 2006-01-17 2013-08-14 私募蛋白质体公司 测试样品的多元化分析
AU2007337810B2 (en) * 2006-12-22 2014-02-13 Archemix Corp. Materials and methods for the generation of transcripts comprising modified nucleotides
NZ578255A (en) * 2007-01-23 2012-06-29 Jostens Inc Method and system for creating customized output
ES2604764T3 (es) * 2007-07-17 2017-03-09 Somalogic, Inc. Análisis de muestras de ensayo
JP2013220095A (ja) * 2012-04-12 2013-10-28 Toyohiro Fujita 苗床
JP2018078250A (ja) 2016-11-11 2018-05-17 株式会社ニューフレアテクノロジー マルチ荷電粒子ビーム描画装置

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Nucleic. Acids. Res., Vol. 22, No. 14, pp. 2817-2822 (1994.07.25.)

Also Published As

Publication number Publication date
EP2069529A2 (en) 2009-06-17
EP2069529A4 (en) 2009-12-30
MX2010000578A (es) 2010-04-30
HK1174366A1 (en) 2013-06-07
HRP20150382T1 (hr) 2015-05-08
CN101809167B (zh) 2015-04-01
JP2019004901A (ja) 2019-01-17
EP2626436A2 (en) 2013-08-14
AU2008275917B2 (en) 2014-08-21
DK2172566T3 (en) 2015-05-04
EP3284832A3 (en) 2018-03-07
CA3022666A1 (en) 2009-01-22
KR20100044787A (ko) 2010-04-30
JP2014050398A (ja) 2014-03-20
WO2009012418A2 (en) 2009-01-22
EP2489743A3 (en) 2012-11-21
AU2008276001A2 (en) 2010-06-03
JP7755619B2 (ja) 2025-10-16
AU2008276001A1 (en) 2009-01-22
HK1213020A1 (en) 2016-06-24
CN101802225A (zh) 2010-08-11
EP2069496A1 (en) 2009-06-17
HK1141839A1 (en) 2010-11-19
CA2693453A1 (en) 2009-01-22
MX2010000533A (es) 2010-04-30
SI2489743T1 (sl) 2015-04-30
MX375114B (es) 2025-03-06
AU2008275917A2 (en) 2010-04-29
EP2172566B2 (en) 2022-05-18
WO2009012420A1 (en) 2009-01-22
MX355017B (es) 2018-04-02
HK1132012A1 (zh) 2010-02-12
ES2533711T3 (es) 2015-04-14
JP2023116788A (ja) 2023-08-22
ES2647587T3 (es) 2017-12-22
US20090098549A1 (en) 2009-04-16
EP2172566B1 (en) 2015-03-04
CN101802225B (zh) 2013-10-30
DK3284832T3 (da) 2023-07-31
ES2537322T5 (es) 2022-09-12
CA2693448A1 (en) 2009-01-22
DK2069529T3 (da) 2013-04-15
EP2336314A1 (en) 2011-06-22
FI3284832T3 (fi) 2023-08-01
CA2696431C (en) 2021-01-05
EP3284832A2 (en) 2018-02-21
JP2010533499A (ja) 2010-10-28
JP5956404B2 (ja) 2016-07-27
EP2076613A1 (en) 2009-07-08
KR101747665B1 (ko) 2017-06-15
JP5404620B2 (ja) 2014-02-05
EP2933340B1 (en) 2017-09-06
WO2009012418A3 (en) 2009-03-05
EP4056711A1 (en) 2022-09-14
PT2172566E (pt) 2015-06-23
DK2489743T3 (en) 2015-03-02
JP2020078311A (ja) 2020-05-28
DK3284832T5 (da) 2024-10-07
EP2933340A1 (en) 2015-10-21
ES2951068T3 (es) 2023-10-17
JP5938080B2 (ja) 2016-06-22
AU2008275917A1 (en) 2009-01-22
ES2604764T3 (es) 2017-03-09
EP2069496A4 (en) 2010-01-27
JP2015062416A (ja) 2015-04-09
US8409795B2 (en) 2013-04-02
CN101809167A (zh) 2010-08-18
ES3045958T3 (en) 2025-12-01
JP6407195B2 (ja) 2018-10-17
JP6491610B2 (ja) 2019-03-27
JP2010533872A (ja) 2010-10-28
EP2069529B1 (en) 2013-01-09
PL2489743T3 (pl) 2015-06-30
WO2009012410A1 (en) 2009-01-22
MX2020009595A (es) 2020-10-07
EP3284832B1 (en) 2023-06-07
DK2172566T4 (da) 2022-06-13
HK1246368A1 (en) 2018-09-07
MX344253B (es) 2016-12-09
JP2019039929A (ja) 2019-03-14
AU2008275915A1 (en) 2009-01-22
HK1133046A1 (en) 2010-03-12
CA2693453C (en) 2018-12-11
AU2008275915A2 (en) 2010-04-22
ES2537322T3 (es) 2015-06-05
CA2696431A1 (en) 2009-01-22
JP7303244B2 (ja) 2023-07-04
NO2933340T3 (cg-RX-API-DMAC7.html) 2018-02-03
JP2021129577A (ja) 2021-09-09
KR20160108584A (ko) 2016-09-19
EP2076613B1 (en) 2016-09-07
AU2008276001A9 (en) 2010-06-10
CN103627792A (zh) 2014-03-12
JP2016122014A (ja) 2016-07-07
JP5684568B2 (ja) 2015-03-11
KR20100058485A (ko) 2010-06-03
JP2016171810A (ja) 2016-09-29
MX341491B (es) 2016-08-23
CN118064441A (zh) 2024-05-24
EP2489743A2 (en) 2012-08-22
CN104593373A (zh) 2015-05-06
CA3022666C (en) 2022-04-19
EP4056711B1 (en) 2025-09-03
PT2489743E (pt) 2015-04-09
CN107090457A (zh) 2017-08-25
EP2626436A3 (en) 2013-10-16
AU2008275915B2 (en) 2013-07-11
EP4056711C0 (en) 2025-09-03
KR101656240B1 (ko) 2016-09-09
EP2172566A1 (en) 2010-04-07
EP2076613A4 (en) 2012-11-28
EP2489743B1 (en) 2015-01-07

Similar Documents

Publication Publication Date Title
KR101634608B1 (ko) 개선된 오프―레이트를 갖는 압타머를 생성하는 방법
US12098370B2 (en) Method for generating aptamers with improved off-rates
US8404830B2 (en) Method for generating aptamers with improved off-rates
AU2015249081B2 (en) Method for generating aptamers with improved off-rates
HK40077705A (en) Method for generating aptamers with improved off-rates
HK1246368B (en) Aptamer with improved rate of dissociation, and non-covalent aptamer-target complex thereof
HK1133046B (en) Method for generating aptamers with improved off-rates
HK1213020B (en) Aptamers with uridines and/or thymidines substituted at the 5-position with a benzyl group
HK1174366B (en) Aptamers with 5-(n-naphthyl)-substituted uridines

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20100118

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20130717

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20141030

Patent event code: PE09021S01D

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20150728

Patent event code: PE09021S01D

E90F Notification of reason for final refusal
PE0902 Notice of grounds for rejection

Comment text: Final Notice of Reason for Refusal

Patent event date: 20160223

Patent event code: PE09021S02D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20160531

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20160623

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20160624

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
FPAY Annual fee payment

Payment date: 20190613

Year of fee payment: 4

PR1001 Payment of annual fee

Payment date: 20190613

Start annual number: 4

End annual number: 4

PR1001 Payment of annual fee

Payment date: 20220614

Start annual number: 7

End annual number: 7

PR1001 Payment of annual fee

Payment date: 20240605

Start annual number: 9

End annual number: 9