JP6552965B2 - 配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化 - Google Patents
配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化 Download PDFInfo
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- JP6552965B2 JP6552965B2 JP2015547545A JP2015547545A JP6552965B2 JP 6552965 B2 JP6552965 B2 JP 6552965B2 JP 2015547545 A JP2015547545 A JP 2015547545A JP 2015547545 A JP2015547545 A JP 2015547545A JP 6552965 B2 JP6552965 B2 JP 6552965B2
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C12N15/1034—Isolating an individual clone by screening libraries
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/10—Vectors comprising a non-peptidic targeting moiety
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Engineering & Computer Science (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
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- General Engineering & Computer Science (AREA)
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- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Ophthalmology & Optometry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Epidemiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (25)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261736527P | 2012-12-12 | 2012-12-12 | |
| US61/736,527 | 2012-12-12 | ||
| US201361748427P | 2013-01-02 | 2013-01-02 | |
| US61/748,427 | 2013-01-02 | ||
| US201361758468P | 2013-01-30 | 2013-01-30 | |
| US61/758,468 | 2013-01-30 | ||
| US201361769046P | 2013-02-25 | 2013-02-25 | |
| US61/769,046 | 2013-02-25 | ||
| US201361791409P | 2013-03-15 | 2013-03-15 | |
| US201361802174P | 2013-03-15 | 2013-03-15 | |
| US61/802,174 | 2013-03-15 | ||
| US61/791,409 | 2013-03-15 | ||
| US201361806375P | 2013-03-28 | 2013-03-28 | |
| US61/806,375 | 2013-03-28 | ||
| US201361814263P | 2013-04-20 | 2013-04-20 | |
| US61/814,263 | 2013-04-20 | ||
| US201361819803P | 2013-05-06 | 2013-05-06 | |
| US61/819,803 | 2013-05-06 | ||
| US201361828130P | 2013-05-28 | 2013-05-28 | |
| US61/828,130 | 2013-05-28 | ||
| US201361836101P | 2013-06-17 | 2013-06-17 | |
| US201361835931P | 2013-06-17 | 2013-06-17 | |
| US61/836,101 | 2013-06-17 | ||
| US61/835,931 | 2013-06-17 | ||
| PCT/US2013/074691 WO2014093635A1 (en) | 2012-12-12 | 2013-12-12 | Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016131404A Division JP6395765B2 (ja) | 2012-12-12 | 2016-07-01 | 配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016501532A JP2016501532A (ja) | 2016-01-21 |
| JP2016501532A5 JP2016501532A5 (enExample) | 2017-02-23 |
| JP6552965B2 true JP6552965B2 (ja) | 2019-07-31 |
Family
ID=49883291
Family Applications (6)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015547545A Active JP6552965B2 (ja) | 2012-12-12 | 2013-12-12 | 配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化 |
| JP2016131404A Active JP6395765B2 (ja) | 2012-12-12 | 2016-07-01 | 配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化 |
| JP2018119670A Active JP6665230B2 (ja) | 2012-12-12 | 2018-06-25 | 配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化 |
| JP2020025253A Active JP7125440B2 (ja) | 2012-12-12 | 2020-02-18 | 配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化 |
| JP2022092414A Pending JP2022113766A (ja) | 2012-12-12 | 2022-06-07 | 配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化 |
| JP2024128853A Pending JP2024163122A (ja) | 2012-12-12 | 2024-08-05 | 配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化 |
Family Applications After (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016131404A Active JP6395765B2 (ja) | 2012-12-12 | 2016-07-01 | 配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化 |
| JP2018119670A Active JP6665230B2 (ja) | 2012-12-12 | 2018-06-25 | 配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化 |
| JP2020025253A Active JP7125440B2 (ja) | 2012-12-12 | 2020-02-18 | 配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化 |
| JP2022092414A Pending JP2022113766A (ja) | 2012-12-12 | 2022-06-07 | 配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化 |
| JP2024128853A Pending JP2024163122A (ja) | 2012-12-12 | 2024-08-05 | 配列操作のための改善された系、方法および酵素組成物のエンジニアリングおよび最適化 |
Country Status (15)
| Country | Link |
|---|---|
| US (6) | US20140186919A1 (enExample) |
| EP (1) | EP2898075B1 (enExample) |
| JP (6) | JP6552965B2 (enExample) |
| KR (1) | KR20150105634A (enExample) |
| CN (2) | CN105209621B (enExample) |
| AU (4) | AU2013359212B2 (enExample) |
| CA (1) | CA2894684A1 (enExample) |
| DK (2) | DK2898075T3 (enExample) |
| ES (2) | ES2786193T3 (enExample) |
| IL (2) | IL300461B1 (enExample) |
| PL (1) | PL2898075T3 (enExample) |
| PT (1) | PT2898075E (enExample) |
| SG (3) | SG11201504519TA (enExample) |
| WO (1) | WO2014093635A1 (enExample) |
| ZA (1) | ZA201504081B (enExample) |
Families Citing this family (770)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2449112A1 (en) | 2009-07-01 | 2012-05-09 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for severe combined immunodeficiency (scid) |
| AR085533A1 (es) | 2011-03-23 | 2013-10-09 | Pioneer Hi Bred Int | Metodos de produccion de un locus de rasgos transgenicos complejo |
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| GB201117313D0 (en) | 2011-10-07 | 2011-11-16 | Gt Biolog Ltd | Bacterium for use in medicine |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| US10039777B2 (en) | 2012-03-20 | 2018-08-07 | Neuro-Lm Sas | Methods and pharmaceutical compositions of the treatment of autistic syndrome disorders |
| ES2683071T3 (es) | 2012-04-25 | 2018-09-24 | Regeneron Pharmaceuticals, Inc. | Direccionamiento mediado por nucleasas con grandes vectores de direccionamiento |
| ES2728782T3 (es) | 2012-05-25 | 2019-10-28 | Univ California | Métodos y composiciones para la modificación de ADN objetivo dirigida por ARN y para la modulación de la transcripción dirigida por ARN |
| US10648001B2 (en) | 2012-07-11 | 2020-05-12 | Sangamo Therapeutics, Inc. | Method of treating mucopolysaccharidosis type I or II |
| HUE051612T2 (hu) | 2012-07-11 | 2021-03-01 | Sangamo Therapeutics Inc | Eljárások és készítmények lizoszomális tárolási betegségek kezelésére |
| CN116622704A (zh) * | 2012-07-25 | 2023-08-22 | 布罗德研究所有限公司 | 可诱导的dna结合蛋白和基因组干扰工具及其应用 |
| EP4397759A3 (en) | 2012-10-23 | 2024-10-09 | Toolgen Incorporated | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof |
| DK3363902T3 (da) | 2012-12-06 | 2020-01-02 | Sigma Aldrich Co Llc | Crispr-baseret genommodifikation og -regulering |
| EP3064585B1 (en) | 2012-12-12 | 2020-02-05 | The Broad Institute, Inc. | Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation |
| EP2931899A1 (en) | 2012-12-12 | 2015-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| CN105209621B (zh) | 2012-12-12 | 2021-05-25 | 布罗德研究所有限公司 | 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化 |
| SG10201707569YA (en) | 2012-12-12 | 2017-10-30 | Broad Inst Inc | Delivery, Engineering and Optimization of Systems, Methods and Compositions for Sequence Manipulation and Therapeutic Applications |
| WO2014093709A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
| KR102955556B1 (ko) | 2012-12-12 | 2026-04-22 | 더 브로드 인스티튜트, 인코퍼레이티드 | 서열 조작을 위한 crispr-cas 성분 시스템, 방법 및 조성물 |
| PT2931898E (pt) | 2012-12-12 | 2016-06-16 | Harvard College | Manipulação e otimização de sistemas, métodos e composições para manipulação de sequências com domínios funcionais |
| IL308158A (en) | 2012-12-17 | 2023-12-01 | Harvard College | Rna-guided human genome engineering |
| WO2014113493A1 (en) | 2013-01-16 | 2014-07-24 | Emory University | Cas9-nucleic acid complexes and uses related thereto |
| MX2015010841A (es) | 2013-02-20 | 2016-05-09 | Regeneron Pharma | Modificacion genetica de ratas. |
| ES2749624T3 (es) * | 2013-03-14 | 2020-03-23 | Caribou Biosciences Inc | Composiciones y métodos de ácidos nucleicos dirigidos a ácido nucleico |
| CN105408483A (zh) | 2013-03-15 | 2016-03-16 | 通用医疗公司 | 遗传和表观遗传调节蛋白至特定基因组基因座的rna引导的靶向 |
| US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| US9234213B2 (en) | 2013-03-15 | 2016-01-12 | System Biosciences, Llc | Compositions and methods directed to CRISPR/Cas genomic engineering systems |
| EP4286517A3 (en) | 2013-04-04 | 2024-03-13 | President and Fellows of Harvard College | Therapeutic uses of genome editing with crispr/cas systems |
| GB201306536D0 (en) | 2013-04-10 | 2013-05-22 | Gt Biolog Ltd | Polypeptide and immune modulation |
| WO2014172470A2 (en) * | 2013-04-16 | 2014-10-23 | Whitehead Institute For Biomedical Research | Methods of mutating, modifying or modulating nucleic acid in a cell or nonhuman mammal |
| DK3456831T3 (da) | 2013-04-16 | 2021-09-06 | Regeneron Pharma | Målrettet modifikation af rottegenom |
| WO2014186686A2 (en) * | 2013-05-17 | 2014-11-20 | Two Blades Foundation | Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases |
| EP3004339B1 (en) * | 2013-05-29 | 2021-07-07 | Cellectis | New compact scaffold of cas9 in the type ii crispr system |
| US20150067922A1 (en) * | 2013-05-30 | 2015-03-05 | The Penn State Research Foundation | Gene targeting and genetic modification of plants via rna-guided genome editing |
| KR20250012194A (ko) | 2013-06-17 | 2025-01-23 | 더 브로드 인스티튜트, 인코퍼레이티드 | 바이러스 구성성분을 사용하여 장애 및 질환을 표적화하기 위한 crispr-cas 시스템 및 조성물의 전달, 용도 및 치료 적용 |
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