JP2015042645A5 - - Google Patents

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JP2015042645A5
JP2015042645A5 JP2014195268A JP2014195268A JP2015042645A5 JP 2015042645 A5 JP2015042645 A5 JP 2015042645A5 JP 2014195268 A JP2014195268 A JP 2014195268A JP 2014195268 A JP2014195268 A JP 2014195268A JP 2015042645 A5 JP2015042645 A5 JP 2015042645A5
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adalimumab
exchange chromatography
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placing
elution
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JP2014195268A 2008-10-20 2014-09-25 プロテインaアフィニティークロマトグラフィーを用いる抗体の単離および精製 Ceased JP2015042645A (ja)

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US19675308P 2008-10-20 2008-10-20
US61/196,753 2008-10-20

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JP2011532332A Division JP5808249B2 (ja) 2008-10-20 2009-10-20 プロテインaアフィニティークロマトグラフィーを用いる抗体の単離および精製

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JP2015042645A JP2015042645A (ja) 2015-03-05
JP2015042645A5 true JP2015042645A5 (enExample) 2015-10-01

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JP2014195268A Ceased JP2015042645A (ja) 2008-10-20 2014-09-25 プロテインaアフィニティークロマトグラフィーを用いる抗体の単離および精製

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US (5) US8895709B2 (enExample)
EP (2) EP2921501A1 (enExample)
JP (2) JP5808249B2 (enExample)
KR (1) KR20110091678A (enExample)
CN (2) CN105111309A (enExample)
AU (4) AU2009347206C1 (enExample)
BR (1) BRPI0920027A2 (enExample)
CA (3) CA2738498A1 (enExample)
ES (1) ES2535734T3 (enExample)
HK (1) HK1209428A1 (enExample)
IL (3) IL211918A (enExample)
MX (3) MX2011004200A (enExample)
NZ (1) NZ592095A (enExample)
RU (1) RU2520838C2 (enExample)
SG (1) SG10201702922VA (enExample)
TW (2) TWI610936B (enExample)
WO (1) WO2010141039A1 (enExample)
ZA (1) ZA201102523B (enExample)

Families Citing this family (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2808343T3 (pl) 2007-12-26 2019-11-29 Xencor Inc Warianty Fc ze zmienionym wiązaniem do FcRn
TWI610936B (zh) * 2008-10-20 2018-01-11 艾伯維有限公司 使用蛋白質a親和性層析進行抗體之分離及純化
TW201028433A (en) 2008-10-20 2010-08-01 Abbott Lab Viral inactivation during purification of antibodies
US8262912B1 (en) * 2009-06-05 2012-09-11 Tenfold Technologies, LLC Isolated bioactive compounds and method of use
US9056265B2 (en) 2009-06-05 2015-06-16 Tenfold Technologies, LLC Isolated bioactive compounds and method of use
KR101764449B1 (ko) * 2009-09-01 2017-08-02 제넨테크, 인크. 변형된 단백질 a 용리를 통한 증진된 단백질 정제
JP6023715B2 (ja) * 2010-10-11 2016-11-09 アッヴィ・バハマズ・リミテッド タンパク質の精製方法
EP2649016B1 (en) * 2010-12-06 2020-06-10 Pall Corporation Continuous processing methods for biological products
US20120238730A1 (en) * 2011-03-15 2012-09-20 Abbott Laboratories Integrated approach to the isolation and purification of antibodies
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
CN103501825B (zh) * 2011-05-02 2017-03-15 免疫医疗公司 用于小体积施用的同种异型选择的抗体的超滤浓缩
US10040855B2 (en) * 2011-05-02 2018-08-07 Millennium Pharmaceuticals, Inc. Formulation for anti-α4β7 antibody
WO2012160536A1 (en) * 2011-05-26 2012-11-29 Dr Reddy's Laboratories Limited Antibody purification
US9809639B2 (en) * 2011-06-08 2017-11-07 Agency For Science, Technology And Research Purification of biological products by constrained cohydration chromatography
US20140288278A1 (en) * 2011-10-31 2014-09-25 Joseph Nti-Gyabaah Chromatography process for resolving heterogeneous antibody aggregates
TW201326193A (zh) * 2011-11-21 2013-07-01 Genentech Inc 抗-c-met抗體之純化
US9334319B2 (en) 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9505833B2 (en) 2012-04-20 2016-11-29 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
AU2013262083A1 (en) 2012-05-14 2014-11-06 Novo Nordisk A/S Stabilised protein solutions
US20140154270A1 (en) * 2012-05-21 2014-06-05 Chen Wang Purification of non-human antibodies using kosmotropic salt enhanced protein a affinity chromatography
WO2013177115A2 (en) * 2012-05-21 2013-11-28 Abbvie Inc. Novel purification of human, humanized, or chimeric antibodies using protein a affinity chromatography
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
CN104334712A (zh) * 2012-06-06 2015-02-04 Emd密理博公司 用溶剂萃取法操作的低有机可萃取深层过滤器介质
SG11201406407TA (en) * 2012-06-29 2014-11-27 Emd Millipore Corp Methods for inactivating viruses during a protein purification process
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
HK1211981A1 (en) 2012-09-02 2016-06-03 Abbvie Inc. Methods to control protein heterogeneity
EP2894159A4 (en) 2012-09-03 2016-01-20 Kaneka Corp MIXING MODE ANTIBODY SAFFINITY MIXTURE AND PURIFICATION PROCESS WITH AND TARGET MOLECULE
AU2013312300A1 (en) 2012-09-07 2015-04-16 Coherus Biosciences, Inc. Stable aqueous formulations of adalimumab
WO2014102814A1 (en) * 2012-12-31 2014-07-03 Intas Biopharmaceuticals Limited Process for the purification of fc fusion proteins
US9650412B2 (en) 2013-03-08 2017-05-16 Genzyme Corporation Integrated continuous manufacturing of therapeutic protein drug substances
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
WO2014159579A1 (en) 2013-03-14 2014-10-02 Abbvie Inc. MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
HK1213919A1 (zh) * 2013-03-14 2016-07-15 Abbvie Inc. 低酸性物質組合物和利用置換色譜技術製備該組合物的方法
PT3395423T (pt) * 2013-03-14 2023-11-14 Amgen Inc Remoção de ligandos escapados de cromatografia de afinidade
WO2014142882A1 (en) * 2013-03-14 2014-09-18 Abbvie Inc. Protein purification using displacement chromatography
JP2016519145A (ja) * 2013-05-15 2016-06-30 メディミューン リミテッド 組換え産生ポリペプチドの精製
KR101569783B1 (ko) * 2013-06-05 2015-11-19 한화케미칼 주식회사 항체의 정제 방법
AR096713A1 (es) 2013-06-25 2016-01-27 Cadila Healthcare Ltd Proceso de purificación para anticuerpos monoclonales
SG11201508835YA (en) 2013-07-12 2015-11-27 Merck Patent Gmbh Removal of fragments from a sample containing a target protein using activated carbon
KR102332302B1 (ko) 2013-09-13 2021-12-01 제넨테크, 인크. 세포주에서 숙주 세포 단백질 및 재조합 폴리펩티드 생성물을 검출 및 정량화하기 위한 조성물 및 방법
SG10201802525QA (en) 2013-09-13 2018-04-27 Genentech Inc Methods and compositions comprising purified recombinant polypeptides
JPWO2015041218A1 (ja) * 2013-09-17 2017-03-02 株式会社カネカ 新規抗体精製方法及びそれから得られる抗体(NovelAntibodyPurificationMethodandAntibodyobtainedtherefrom)、並びに陽イオン交換基を用いた新規抗体精製法及びそれから得られる抗体(NovelAntibodyPurificationmethodusingCationExchangerandAntibodyobtainedtherefrom)
WO2015051293A2 (en) 2013-10-04 2015-04-09 Abbvie, Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
WO2015057910A1 (en) 2013-10-16 2015-04-23 Oncobiologics, Inc. Buffer formulations for enhanced antibody stability
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
EP4671270A3 (en) * 2013-12-27 2026-03-18 Chugai Seiyaku Kabushiki Kaisha Method for purifying antibody having low isoelectric point
TWI671312B (zh) 2014-01-17 2019-09-11 美商健臻公司 無菌層析法及製法
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
JP6293907B2 (ja) 2014-03-10 2018-03-14 リヒター ゲデオン エヌワイアールティー. プレクリーニング工程を用いた免疫グロブリンの精製
US11357857B2 (en) 2014-06-20 2022-06-14 Comera Life Sciences, Inc. Excipient compounds for protein processing
US20160074515A1 (en) 2014-06-20 2016-03-17 Reform Biologics, Llc Viscosity-reducing excipient compounds for protein formulations
US10478498B2 (en) 2014-06-20 2019-11-19 Reform Biologics, Llc Excipient compounds for biopolymer formulations
EP3145951A1 (en) 2014-06-24 2017-03-29 InSight Biopharmaceuticals Ltd. Methods of purifying antibodies
US10329323B2 (en) 2014-07-25 2019-06-25 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Method for purifying antibodies using PBS
JP6630036B2 (ja) * 2014-09-30 2020-01-15 Jsr株式会社 標的物の精製方法、及び、ミックスモード用担体
AR102198A1 (es) 2014-10-09 2017-02-08 Regeneron Pharma Proceso para reducir partículas subvisibles en una formulación farmacéutica
CN105566442A (zh) * 2014-10-11 2016-05-11 江苏泰康生物医药有限公司 一种降低单克隆抗体生产中宿主细胞蛋白含量的亲和纯化工艺
US10696735B2 (en) 2015-01-21 2020-06-30 Outlook Therapeutics, Inc. Modulation of charge variants in a monoclonal antibody composition
SG10201908027QA (en) 2015-03-13 2019-10-30 Bristol Myers Squibb Co Use of alkaline washes during chromatography to remove impurities
MA42613A (fr) 2015-08-13 2018-06-20 Amgen Inc Filtration en profondeur chargée de protéines de liaison à un antigène
JP7091238B2 (ja) * 2015-08-20 2022-06-27 ジェネンテック, インコーポレイテッド Fkpaの精製、及び組換えポリペプチドの産生のためのその使用
US11229702B1 (en) 2015-10-28 2022-01-25 Coherus Biosciences, Inc. High concentration formulations of adalimumab
EP3411401A1 (en) 2016-02-03 2018-12-12 Oncobiologics, Inc. Buffer formulations for enhanced antibody stability
US11186858B1 (en) 2016-03-15 2021-11-30 Fresenius Kabi Deutschland Gmbh Methods for increasing biosimilarity
CN108883345B (zh) 2016-04-01 2022-09-13 Ucb生物制药私人有限公司 用于蛋白纯化的方法
IL290358B2 (en) * 2016-04-14 2024-03-01 Lonza Ag Preparations and methods for detecting host cell proteins
US11071782B2 (en) 2016-04-20 2021-07-27 Coherus Biosciences, Inc. Method of filling a container with no headspace
MX2019000935A (es) 2016-08-16 2019-07-04 Regeneron Pharma Metodos para cuantificar anticuerpos individuales de una mezcla.
IL312194B1 (en) 2016-10-25 2026-01-01 Regeneron Pharma Methods and systems for analyzing chromatography data
KR102729723B1 (ko) * 2016-12-09 2024-11-15 글리크닉 인코포레이티드 다합체화 스트라도머인 gl-2045의 제조 최적화
JP7519774B2 (ja) 2016-12-09 2024-07-22 グリックニック インコーポレイテッド 多価fc化合物を用いる炎症性疾患の治療方法
EP3824906A1 (en) 2016-12-21 2021-05-26 Amgen Inc. Anti-tnf alpha antibody formulations
US20200131251A1 (en) 2016-12-23 2020-04-30 Serum Institute Of India Private Limited Improved methods for enhancing antibody productivity in mammalian cell culture and minimizing aggregation during downstream, formulation processes and stable antibody formulations obtained thereof
KR102578087B1 (ko) 2017-08-17 2023-09-18 저스트-에보텍 바이오로직스, 아이엔씨. 숙주세포 갈렉틴(galectins) 및 다른 오염물(contaminant)로부터 글리코실화 단백질을 정제하는 방법
TWI799441B (zh) * 2017-08-23 2023-04-21 日商Jsr股份有限公司 層析用載體﹑配位子固定載體﹑層析管柱﹑標的物質之純化方法,及層析用載體之製造方法
MX2020002850A (es) 2017-09-19 2020-07-24 Regeneron Pharma Metodos para reducir la formacion de particulas y composiciones formadas a partir de estas.
WO2019086463A1 (en) 2017-10-30 2019-05-09 Baxalta GmbH Environmentally compatible detergents for inactivation of lipid-enveloped viruses
WO2019126554A1 (en) * 2017-12-21 2019-06-27 Genzyme Corporation Methods for enhanced removal of impurities during protein a chromatography
WO2019183334A1 (en) 2018-03-21 2019-09-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
CN108239146A (zh) * 2018-03-26 2018-07-03 江苏中新医药有限公司 一种高纯度rhNGF的制备方法
KR102837419B1 (ko) * 2018-04-20 2025-07-23 얀센 바이오테크 인코포레이티드 항-il12/il23 항체 조성물을 생성하기 위한 제조 방법에 있어서의 크로마토그래피 컬럼 적격성 평가
TWI853823B (zh) 2018-07-02 2024-09-01 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
AU2019332764B2 (en) * 2018-08-31 2025-05-29 Genzyme Corporation Sterile chromatography resin and use thereof in manufacturing processes
KR102337683B1 (ko) * 2018-09-21 2021-12-13 주식회사 녹십자 고효율 항-tfpi 항체 조성물
CN111072773A (zh) * 2018-10-19 2020-04-28 菲鹏生物股份有限公司 抗体的纯化方法、抗体和应用
CN111153993B (zh) * 2018-11-07 2024-06-28 正大天晴药业集团南京顺欣制药有限公司 抗TNF-α单克隆抗体的制备方法
CA3120023A1 (en) * 2018-11-29 2020-06-04 Reform Biologics, Llc Excipient compounds for protein processing
GB201904741D0 (en) * 2019-04-04 2019-05-22 Glaxosmithkline Ip Dev Ltd Novel process
US20220306727A1 (en) * 2019-06-05 2022-09-29 Seagen Inc. Methods of Purifying Masked Antibodies
EP4653865A3 (en) * 2019-09-24 2026-02-18 Regeneron Pharmaceuticals, Inc. Systems and methods for chromatography use and regeneration
CN116348486A (zh) * 2020-10-02 2023-06-27 伊莱利利公司 在蛋白质纯化过程中降低宿主细胞蛋白质含量的方法
WO2023284073A1 (zh) * 2021-07-13 2023-01-19 江苏荃信生物医药股份有限公司 降低单克隆抗体生产中宿主细胞蛋白含量的亲和纯化方法、抗人ifnar1单克隆抗体浓缩溶液的制备方法以及液体制剂
CN113501857A (zh) * 2021-08-17 2021-10-15 沈阳百发科技有限公司 一种高活性重组蛋白的制备方法
CN114292328B (zh) * 2021-12-30 2025-07-25 武汉原谷生物科技有限责任公司 一种来源于美洲羊驼中天然抗体的纯化方法及其应用
WO2023141612A2 (en) * 2022-01-24 2023-07-27 University Of Florida Research Foundation, Incorporated Point of care testing system for antithrombin iii
CN115073590A (zh) * 2022-07-08 2022-09-20 杭州艾策生物技术有限公司 一种减少单克隆抗体氧化杂质的深层过滤方法
WO2025059162A1 (en) 2023-09-11 2025-03-20 Dana-Farber Cancer Institute, Inc. Car-engager containing il-2 variants to enhance the functionality of car t cells

Family Cites Families (208)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE30985E (en) 1978-01-01 1982-06-29 Serum-free cell culture media
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US5179017A (en) 1980-02-25 1993-01-12 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4510245A (en) 1982-11-18 1985-04-09 Chiron Corporation Adenovirus promoter system
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
JPS59156299A (ja) 1983-02-28 1984-09-05 Noda Sangyo Kagaku Kenkyusho N−アセチルヘキソサミンの定量法及びその定量用試薬
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4534972A (en) 1983-03-29 1985-08-13 Miles Laboratories, Inc. Protein compositions substantially free from infectious agents
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
DD266710A3 (de) 1983-06-06 1989-04-12 Ve Forschungszentrum Biotechnologie Verfahren zur biotechnischen Herstellung van alkalischer Phosphatase
US4767704A (en) 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
EP0154316B1 (en) 1984-03-06 1989-09-13 Takeda Chemical Industries, Ltd. Chemically modified lymphokine and production thereof
US4879231A (en) 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US5168062A (en) 1985-01-30 1992-12-01 University Of Iowa Research Foundation Transfer vectors and microorganisms containing human cytomegalovirus immediate-early promoter-regulatory DNA sequence
GB8516415D0 (en) 1985-06-28 1985-07-31 Celltech Ltd Culture of animal cells
US4968615A (en) 1985-12-18 1990-11-06 Ciba-Geigy Corporation Deoxyribonucleic acid segment from a virus
US5225539A (en) 1986-03-27 1993-07-06 Medical Research Council Recombinant altered antibodies and methods of making altered antibodies
US4927762A (en) 1986-04-01 1990-05-22 Cell Enterprises, Inc. Cell culture medium with antioxidant
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
US5045468A (en) * 1986-12-12 1991-09-03 Cell Enterprises, Inc. Protein-free culture medium which promotes hybridoma growth
US5476996A (en) 1988-06-14 1995-12-19 Lidak Pharmaceuticals Human immune system in non-human animal
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
DE68925971T2 (de) 1988-09-23 1996-09-05 Cetus Oncology Corp Zellenzuchtmedium für erhöhtes zellenwachstum, zur erhöhung der langlebigkeit und expression der produkte
US6048728A (en) * 1988-09-23 2000-04-11 Chiron Corporation Cell culture medium for enhanced cell growth, culture longevity, and product expression
JP2989002B2 (ja) 1988-12-22 1999-12-13 キリン―アムジエン・インコーポレーテツド 化学修飾顆粒球コロニー刺激因子
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
EP0402226A1 (en) 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Transformation vectors for yeast yarrowia
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
US5110913A (en) * 1990-05-25 1992-05-05 Miles Inc. Antibody purification method
US5096816A (en) * 1990-06-05 1992-03-17 Cetus Corporation In vitro management of ammonia's effect on glycosylation of cell products through pH control
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
JPH06508511A (ja) 1990-07-10 1994-09-29 ケンブリッジ アンティボディー テクノロジー リミティド 特異的な結合ペアーの構成員の製造方法
US5122469A (en) 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
DE69129154T2 (de) 1990-12-03 1998-08-20 Genentech, Inc., South San Francisco, Calif. Verfahren zur anreicherung von proteinvarianten mit geänderten bindungseigenschaften
DE69233769D1 (de) 1991-03-01 2009-09-24 Dyax Corp Chimäres Protein mit Mikroprotein mit zwei oder mehr Disulfidbindungen und Ausgestaltungen davon
ATE414768T1 (de) 1991-04-10 2008-12-15 Scripps Research Inst Bibliotheken heterodimerer rezeptoren mittels phagemiden
DE4122599C2 (de) 1991-07-08 1993-11-11 Deutsches Krebsforsch Phagemid zum Screenen von Antikörpern
EP0666312A1 (en) * 1994-02-08 1995-08-09 Wolfgang A. Renner Process for the improvement of mammalian cell growth
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
US5853714A (en) * 1995-03-27 1998-12-29 Genetics Institute, Inc. Method for purification of IL-12
US5641870A (en) * 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
US6656466B1 (en) * 1995-06-06 2003-12-02 Genetech, Inc. Human tumor necrosis factor—immunoglobulin(TNFR1-IgG1) chimera composition
US5721121A (en) * 1995-06-06 1998-02-24 Genentech, Inc. Mammalian cell culture process for producing a tumor necrosis factor receptor immunoglobulin chimeric protein
US5705364A (en) * 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
JP4306813B2 (ja) * 1995-09-19 2009-08-05 アスビオファーマ株式会社 動物細胞の新規培養方法
ATE222598T1 (de) * 1996-04-19 2002-09-15 Nestle Sa Menschliche immortalisierte colon- epithelialzellen
AU5082698A (en) 1996-10-23 1998-05-15 Oravax, Inc Heat inactivation of viruses in antibody preparations
WO1998022137A1 (en) * 1996-11-15 1998-05-28 The Kennedy Institute Of Rheumatology SUPPRESSION OF TNF α AND IL-12 IN THERAPY
DE69739673D1 (de) * 1996-11-27 2009-12-31 Genentech Inc Affinitätsreinigung von Polypeptid-Proteinen auf einer Protein A Matrix
US6955917B2 (en) * 1997-06-20 2005-10-18 Bayer Healthcare Llc Chromatographic method for high yield purification and viral inactivation of antibodies
IL121900A (en) 1997-10-07 2001-12-23 Omrix Biopharmaceuticals Ltd A method for the purification of immunoglobulins
US20020045207A1 (en) * 1997-10-31 2002-04-18 Lynne A. Krummen Glycoprotein production process
WO1999032605A1 (en) 1997-12-19 1999-07-01 Novo Nordisk A/S Method for producing heterologous proteins in eukaryotic cells on an industrial scale using nucleotide-manipulating agents
ES2292682T3 (es) * 1998-05-06 2008-03-16 Genentech, Inc. Purificacion de anticuerpos mediante cromatografia de intercambio ionico.
US6528286B1 (en) * 1998-05-29 2003-03-04 Genentech, Inc. Mammalian cell culture process for producing glycoproteins
US6914128B1 (en) 1999-03-25 2005-07-05 Abbott Gmbh & Co. Kg Human antibodies that bind human IL-12 and methods for producing
CA2384880C (en) * 1999-09-27 2010-09-21 Genentech, Inc. Methods for making recombinant proteins using apoptosis inhibitors
DE60120356T2 (de) * 2000-02-08 2007-06-14 Genentech, Inc., South San Francisco Verbesserte galactosylierung von rekombinanten proteinen
TWI373343B (en) 2000-02-10 2012-10-01 Abbott Gmbh & Co Kg Antibodies that bind human interleukin-18 and methods of making and using
US7598055B2 (en) 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
EP1175931A1 (en) * 2000-07-25 2002-01-30 Computer Cell Culture Center S.A. Integration of high cell density bioreactor operation with ultra fast on-line downstream processing
AU2001294520A1 (en) * 2000-08-21 2002-03-04 Clonex Development, Inc. Methods and compositions for increasing protein yield from a cell culture
US6693173B2 (en) * 2000-12-26 2004-02-17 Alpha Therapeutic Corporation Method to remove citrate and aluminum from proteins
US20030096414A1 (en) * 2001-03-27 2003-05-22 Invitrogen Corporation Culture medium for cell growth and transfection
CN1789416B (zh) 2001-05-11 2011-11-16 协和发酵麒麟株式会社 含人抗体λ轻链基因的人类人工染色体
WO2002098531A1 (en) * 2001-06-05 2002-12-12 Genetics Institute, Llc Methods for purifying highly anionic proteins
RU2321630C2 (ru) * 2001-08-03 2008-04-10 Гликарт Биотекнолоджи АГ Гликозилированные антитела (варианты), обладающие повышенной антителозависимой клеточной цитотоксичностью
HUP0402158A2 (hu) * 2001-10-02 2005-01-28 Novo Nordisk Health Care Ag Rekombináns fehérjék előállítási eljárása eukarióta sejtekben
US20030157108A1 (en) * 2001-10-25 2003-08-21 Genentech, Inc. Glycoprotein compositions
IL161858A0 (en) 2001-11-28 2005-11-20 Sandoz Ag Cell culture process
MXPA04006053A (es) * 2001-12-21 2005-03-31 Immunex Corp Metodos para purificacion de proteina.
US20030201229A1 (en) * 2002-02-04 2003-10-30 Martin Siwak Process for prefiltration of a protein solution
ATE417858T1 (de) * 2002-02-05 2009-01-15 Genentech Inc Proteinaufreinigung
CA2417689C (en) * 2002-03-05 2006-05-09 F. Hoffmann-La Roche Ag Improved methods for growing mammalian cells in vitro
US20030190710A1 (en) * 2002-03-28 2003-10-09 Devries Ruth L. Control of glycoforms in IgG
US20040132140A1 (en) * 2002-04-09 2004-07-08 Kyowa Hakko Kogyo Co., Ltd. Production process for antibody composition
RU2304587C2 (ru) * 2002-06-10 2007-08-20 Гуд Байотек Копэрейшн СПОСОБ СЕЛЕКТИВНОГО ВЫДЕЛЕНИЯ АНТИТЕЛ IgY ИЗ ЯИЧНОГО ЖЕЛТКА ПТИЦ ОТРЯДА ГУСЕОБРАЗНЫХ (ВАРИАНТЫ)
ES2285188T3 (es) * 2002-07-15 2007-11-16 Immunex Corporation Metodo y medios para controlar sialilacion de proteinas producidas por celulas de mamifero.
AR040603A1 (es) * 2002-07-19 2005-04-13 Abbott Lab S A El uso de un anticuerpo anti-tnfalfa neutralizador de alta afinidad en la fabricacion de un medicamento y conjunto de elementos
US7067279B1 (en) * 2002-08-23 2006-06-27 Immunex Corporation Cell culture performance with betaine
US6974681B1 (en) * 2002-08-23 2005-12-13 Immunex Corporation Cell culture performance with vanadate
WO2004026427A2 (en) 2002-09-17 2004-04-01 Gtc Biotherapeutics, Inc. Isolation of immunoglobulin molecules that lack inter-heavy chain disulfide bonds
US7208585B2 (en) * 2002-09-18 2007-04-24 Genencor International, Inc. Protein purification
US20040162414A1 (en) 2002-11-22 2004-08-19 Santora Ling C. Method for reducing or preventing modification of a polypeptide in solution
JP4541157B2 (ja) * 2002-12-23 2010-09-08 ブリストル−マイヤーズ スクイブ カンパニー タンパク質を製造するための哺乳類細胞培養法
DE60335024D1 (de) * 2002-12-23 2010-12-30 Bristol Myers Squibb Co Produktqualitätsverbesserung in säugerzellkulturverfahrenzur proteinproduktion
PT1623019E (pt) * 2003-05-15 2010-07-20 Wyeth Llc ALIMENTAÆO COM GLUCOSE DE MODO LIMITADO PARA CULTURA DE CéLULAS ANIMAIS
PT3095793T (pt) * 2003-07-28 2020-05-04 Genentech Inc Redução da lixiviação de proteína a durante a cromatografia por afinidade com proteína a
GB2404665B (en) * 2003-08-08 2005-07-06 Cambridge Antibody Tech Cell culture
WO2005035748A1 (en) * 2003-10-10 2005-04-21 Novo Nordisk Health Care Ag Method for large-scale production of a polypeptide in eukaryote cells and a culture vessel suitable therefor
PT1678208E (pt) * 2003-10-27 2013-07-10 Wyeth Llc Remoção de agregados de elevado peso molecular utilizando cromatografia de hidroxiapatite
US8084032B2 (en) 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
SE0400886D0 (sv) * 2004-04-02 2004-04-02 Amersham Biosciences Ab Process of purification
US20060127950A1 (en) * 2004-04-15 2006-06-15 Massachusetts Institute Of Technology Methods and products related to the improved analysis of carbohydrates
EP1745141B2 (en) 2004-05-04 2019-09-25 Novo Nordisk Health Care AG O-linked glycoforms of faktor vii and method to manufacture them
TWI384069B (zh) 2004-08-27 2013-02-01 Pfizer Ireland Pharmaceuticals 多胜肽之製法
EP1807101B1 (en) * 2004-09-30 2016-04-13 Bayer Healthcare LLC Devices and methods for integrated continuous manufacturing of biological molecules
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
JP2006143601A (ja) * 2004-11-16 2006-06-08 Yamato Yakuhin Kk 血液粘度低下剤
US8728828B2 (en) * 2004-12-22 2014-05-20 Ge Healthcare Bio-Sciences Ab Purification of immunoglobulins
US8530192B2 (en) 2005-02-11 2013-09-10 Novo Nordisk Healthcare Ag Production of a polypeptide in a serum-free cell culture liquid containing plant protein hydrolysate
WO2006099308A2 (en) * 2005-03-11 2006-09-21 Wyeth A method of weak partitioning chromatography
EP1869067A1 (en) 2005-04-11 2007-12-26 Medarex, Inc. Protein purification using hcic amd ion exchange chromatography
WO2006113743A2 (en) * 2005-04-18 2006-10-26 Massachusetts Institute Of Technology Compositions and methods for rna interference with sialidase expression and uses thereof
US7247457B2 (en) * 2005-04-26 2007-07-24 United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Detection and identification of enteroviruses by semi-nested amplification of the enterovirus VP1 protein
EP1888638A2 (en) * 2005-06-03 2008-02-20 Genentech, Inc. Method of producing antibodies with modified fucosylation level
ES2776657T3 (es) * 2005-06-14 2020-07-31 Amgen Inc Formulaciones de proteínas autotamponantes
ES2402650T3 (es) * 2005-06-17 2013-05-07 Janssen Alzheimer Immunotherapy Métodos de purificación de anticuerpos anti A beta
SG155183A1 (en) * 2005-08-26 2009-09-30 Ares Trading Sa Process for the preparation of glycosylated interferon beta
AR058140A1 (es) * 2005-10-24 2008-01-23 Wyeth Corp Metodo de produccion proteica utilizando compuestos anti-senescencia
US7972810B2 (en) * 2005-12-08 2011-07-05 Amgen Inc. Production of glycoproteins using manganese
US20070190057A1 (en) 2006-01-23 2007-08-16 Jian Wu Methods for modulating mannose content of recombinant proteins
MX2008012896A (es) * 2006-04-05 2008-10-14 Abbott Biotech Ltd Purificacion de anticuerpos.
RU2008150314A (ru) * 2006-05-19 2010-06-27 Гликофи, Инк. (Us) Композиции эритропоэтина
WO2009017491A1 (en) * 2006-06-14 2009-02-05 Smithkline Beecham Corporation Methods for purifying antibodies using ceramic hydroxyapatite
WO2008007280A2 (en) 2006-06-28 2008-01-17 Koninklijke Philips Electronics N.V. Assay system and method
DK3255141T3 (da) 2006-07-13 2022-01-31 Wyeth Llc Fremstilling af antistoffer med forbedret glycosyleringsmønster
FI2061803T4 (fi) * 2006-08-28 2023-03-14 Process for the purification of fc-containing proteins
CL2007002615A1 (es) * 2006-09-08 2008-04-18 Wyeth Corp Metodos para aislar o purificar un producto que comprende poner el producto enlazado en contacto con al menos una solucion de lavado que comprende arginina y luego eluir el producto; y dicho producto.
US8911964B2 (en) * 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
BRPI0716762A2 (pt) * 2006-09-13 2013-09-24 Abbott Lab melhorias da cultura celular
WO2008036600A2 (en) * 2006-09-18 2008-03-27 Genentech, Inc. Methods of protein production
WO2008036899A2 (en) 2006-09-22 2008-03-27 Amgen Inc. Methods for removing viral contaminants during protein purification
EP2395077A1 (en) * 2006-11-03 2011-12-14 Wyeth LLC Glycolysis-inhibiting substances in cell culture
WO2008068879A1 (en) 2006-12-06 2008-06-12 Jcr Pharmaceuticals Co., Ltd. Method for production of human erythropoietin
US8163886B2 (en) * 2006-12-21 2012-04-24 Emd Millipore Corporation Purification of proteins
JP5586235B2 (ja) * 2007-03-02 2014-09-10 ワイス・エルエルシー ポリペプチドの産生のための細胞培養における銅およびグルタミン酸塩の使用
US7935808B2 (en) * 2007-03-30 2011-05-03 Abbott Laboratories Recombinant expression vector elements (rEVEs) for enhancing expression of recombinant proteins in host cells
AU2008232903B9 (en) * 2007-03-30 2013-09-05 Medimmune Llc Antibodies with decreased deamidation profiles
CA2887752C (en) * 2007-04-03 2020-03-24 Vib Vzw Glycosylation of molecules
CA2682738A1 (en) 2007-04-16 2008-10-23 Momenta Pharmaceuticals, Inc. Defined glycoprotein products and related methods
TW200902708A (en) * 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
EP1988101A1 (en) 2007-05-04 2008-11-05 Novo Nordisk A/S Improvement of factor VIII polypeptide titers in cell cultures
WO2008135498A2 (en) 2007-05-04 2008-11-13 Novo Nordisk A/S Prevention of protein degradation in mammalian cell cultures
WO2008141207A1 (en) 2007-05-11 2008-11-20 Amgen Inc. Improved feed media
WO2008154014A2 (en) * 2007-06-11 2008-12-18 Amgen Inc. A method for culturing mammalian cells to improve recombinant protein production
MX354993B (es) * 2007-07-09 2018-03-28 Genentech Inc Prevención de reducción de enlaces de disulfuro durante la producción recombinante de polipéptidos.
ES2657055T3 (es) 2007-08-09 2018-03-01 Wyeth Llc Uso de perfusión para mejorar la producción de un cultivo de células alimentado por lotes en biorreactores
BRPI0817182A2 (pt) 2007-10-30 2015-03-17 Genentech Inc Método para purificar um anticorpo e composições
WO2009058769A1 (en) 2007-10-30 2009-05-07 Schering Corporation Purification of antibodies containing hydrophobic variants
TWM332870U (en) * 2007-11-22 2008-05-21 Delta Electronics Inc Side-edge type backlight module
NZ622583A (en) * 2007-11-30 2015-08-28 Abbvie Biotechnology Ltd Protein formulations and methods of making same
AU2008345231B2 (en) 2007-12-27 2014-09-11 Takeda Pharmaceutical Company Limited Cell culture processes
WO2009092014A1 (en) * 2008-01-18 2009-07-23 Gagnon Peter S Enhanced purification of antibodies and antibody fragments by apatite chromatography
AU2009223054A1 (en) * 2008-03-11 2009-09-17 Genentech, Inc. Antibodies with enhanced ADCC function
PT2271382E (pt) * 2008-04-15 2013-05-07 Grifols Therapeutics Inc Ultrafiltração em dois andares/diafiltração
ES2458541T5 (en) * 2008-05-02 2025-08-04 Seagen Inc Methods and compositions for making antibodies and antibody derivatives with reduced core fucosylation
WO2010016943A2 (en) 2008-08-08 2010-02-11 Biogen Idec Ma Inc. Nutrient monitoring and feedback control for increased bioproduct production
CN102149724B (zh) * 2008-08-14 2014-04-09 健泰科生物技术公司 使用原地蛋白质置换离子交换膜层析清除污染物的方法
WO2010036443A1 (en) 2008-09-26 2010-04-01 Eureka Therapeutics, Inc. Cell lines and proteins with variant glycosylation pattern
AU2009307735B2 (en) 2008-10-20 2014-12-04 Abbvie Inc. Antibodies that bind to IL-12 and methods of purifying the same
KR20110071011A (ko) 2008-10-20 2011-06-27 아보트 러보러터리즈 Il-18에 결합하는 항체 및 이의 정제 방법
TWI610936B (zh) * 2008-10-20 2018-01-11 艾伯維有限公司 使用蛋白質a親和性層析進行抗體之分離及純化
TW201028433A (en) 2008-10-20 2010-08-01 Abbott Lab Viral inactivation during purification of antibodies
US8058407B2 (en) * 2008-10-31 2011-11-15 Wyeth Llc Purification of acidic proteins using ceramic hydroxyapatite chromatography
SG172004A1 (en) * 2008-12-09 2011-07-28 Hoffmann La Roche Method for obtaining an excipient-free antibody solution
JP2012513425A (ja) 2008-12-22 2012-06-14 エフ.ホフマン−ラ ロシュ アーゲー 免疫グロブリンの精製
EP2373676B1 (en) 2009-01-08 2017-04-19 GE Healthcare BioProcess R&D AB Separation method using single polymer phase systems
WO2010102114A1 (en) 2009-03-05 2010-09-10 Biogen Idec Ma Inc. Purification of immunoglobulins
EP2412817B2 (en) * 2009-03-27 2019-06-05 Asahi Kasei Medical Co., Ltd. Method for removing viruses in high-concentration monoclonal antibody solution
AR076288A1 (es) 2009-04-13 2011-06-01 Bristol Myers Squibb Co Purificacion de proteinas por precipitacion con citrato
AU2010240569A1 (en) * 2009-04-20 2011-10-20 Pfizer Inc. Control of protein glycosylation and compositions and methods relating thereto
US8969043B2 (en) * 2009-05-28 2015-03-03 Boehringer Ingelheim International Gmbh Method for a rational cell culturing process
EP2438185A4 (en) * 2009-06-05 2016-10-05 Momenta Pharmaceuticals Inc METHODS FOR MODULATING FUCOSYLATION OF GLYCOPROTEINS
CN107043703B (zh) 2009-07-06 2020-03-27 弗·哈夫曼-拉罗切有限公司 真核细胞的培养方法
BRPI1010119A2 (pt) 2009-07-24 2016-03-15 Hoffmann La Roche "método de produção de composição de anticorpo e uso de cromatografia de troca de íons analítica"
WO2011015926A1 (en) 2009-08-03 2011-02-10 Avesthagen Limited A process of fermentation, purification and production of recombinant soluble tumour necrosis factor alfa receptor (tnfr) - human igg fc fusion protein
SG178276A1 (en) 2009-08-06 2012-03-29 Genentech Inc Method to improve virus removal in protein purification
WO2011019622A1 (en) * 2009-08-14 2011-02-17 Genentech, Inc. Cell culture methods to make antibodies with enhanced adcc function
WO2011024025A1 (en) 2009-08-28 2011-03-03 Avesthagen Limited An erythropoietin analogue and a method thereof
KR101764449B1 (ko) 2009-09-01 2017-08-02 제넨테크, 인크. 변형된 단백질 a 용리를 통한 증진된 단백질 정제
US9540426B2 (en) 2009-10-06 2017-01-10 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
EP3037104B1 (en) * 2009-10-20 2020-05-27 AbbVie Inc. Isolation and purification of anti-il-13 antibodies using protein a affinity chromatography
EP2490780A4 (en) * 2009-10-20 2014-04-09 Merck Sharp & Dohme USE OF A MIXED MODE CHROMATOGRAPHY FOR THE DETECTION AND PURIFICATION OF BASIC ANTIBODY PRODUCTS
US9096879B2 (en) 2009-11-24 2015-08-04 Biogen Ma Inc. Method of supplementing culture media to prevent undesirable amino acid substitutions
WO2011069056A2 (en) 2009-12-04 2011-06-09 Momenta Pharmaceuticals, Inc. Antennary fucosylation in glycoproteins from cho cells
US8277649B2 (en) 2009-12-14 2012-10-02 General Electric Company Membranes and associated methods for purification of antibodies
CA2784278C (en) * 2009-12-18 2019-02-26 Novartis Ag Wash solution and method for affinity chromatography
KR101405463B1 (ko) * 2010-01-15 2014-06-27 그래핀스퀘어 주식회사 기체 및 수분 차단용 그래핀 보호막, 이의 형성 방법 및 그의 용도
CN102858988A (zh) * 2010-04-06 2013-01-02 赫里开发公司 通过使用具有递减极性的溶剂从含油材料连续提取油和蛋白质材料
US9921210B2 (en) 2010-04-07 2018-03-20 Momenta Pharmaceuticals, Inc. High mannose glycans
ES2533074T3 (es) 2010-04-26 2015-04-07 Novartis Ag Medio de cultivo celular mejorado
RU2577972C2 (ru) 2010-04-26 2016-03-20 Новартис Аг Способ получения рекомбинантного полипептида
PL2576580T3 (pl) 2010-05-28 2017-03-31 F.Hoffmann-La Roche Ag Zmniejszenie poziomu mleczanu i zwiększenie wytwarzania polipeptydu przez obniżenie poziomu ekspresji dehydrogenazy mleczanowej i kinazy dehydrogenazy pirogronianowej
WO2012019160A1 (en) 2010-08-05 2012-02-09 Amgen Inc. Dipeptides to enhance yield and viability from cell cultures
WO2012030512A1 (en) 2010-09-03 2012-03-08 Percivia Llc. Flow-through protein purification process
CA2810909A1 (en) * 2010-09-20 2012-03-29 Abbvie Inc. Purification of antibodies using simulated moving bed chromatography
JP6023715B2 (ja) * 2010-10-11 2016-11-09 アッヴィ・バハマズ・リミテッド タンパク質の精製方法
KR101898302B1 (ko) 2010-10-15 2018-09-13 제이씨알 파마 가부시키가이샤 당사슬의 비환원 말단이 만노오스 잔기인 당 단백질의 제조 방법
EP2450375A1 (en) 2010-11-09 2012-05-09 Sandoz Gmbh Cell culture medium and process for protein expression, said medium and process comprising a PAM inhibitor
US20120149878A1 (en) * 2010-12-08 2012-06-14 Gillespie Ronald Protein purification
KR101574864B1 (ko) 2010-12-21 2015-12-11 에프. 호프만-라 로슈 아게 이소폼이 농축된 항체 제제 및 그의 제조 방법
WO2012120500A2 (en) 2011-03-06 2012-09-13 Merck Serono S.A. Low fucose cell lines and uses thereof
TR201810703T4 (tr) 2011-03-25 2018-08-27 Hoffmann La Roche Yeni protein saflaştırma yöntemleri.
EP2511293A1 (en) 2011-04-13 2012-10-17 LEK Pharmaceuticals d.d. A method for controlling the main complex N-glycan structures and the acidic variants and variability in bioprocesses producing recombinant proteins
WO2012145682A1 (en) 2011-04-21 2012-10-26 Amgen Inc. A method for culturing mammalian cells to improve recombinant protein production
CN105189735B (zh) 2011-04-29 2019-04-12 拜康研究有限公司 在培养期间减少乳酸累积的方法和生产多肽的方法
US20120283419A1 (en) 2011-05-03 2012-11-08 Avantor Performance Materials, Inc. Separation of protein monomers from aggregates by solid weak anion exchange support functionalized with amine moieties
DK2707383T3 (en) 2011-05-13 2018-07-23 Biogen Ma Inc PROCEDURES FOR PREVENTION AND REMOVAL OF TRISULPHIDE BINDINGS
CA2838695C (en) 2011-07-01 2017-02-14 Amgen Inc. Mammalian cell culture
WO2013006461A1 (en) 2011-07-01 2013-01-10 Biogen Idec Ma Inc. Cholesterol-based media supplementals for cell culture
EP2729561A4 (en) 2011-07-08 2015-06-24 Momenta Pharmaceuticals Inc CELL CULTURE METHOD
WO2013013013A2 (en) 2011-07-21 2013-01-24 Alnylam Pharmaceuticals, Inc. Compositions and methods for producing modified glycoproteins
CA2851053A1 (en) 2011-10-19 2013-04-25 Roche Glycart Ag Separation method for fucosylated antibodies
US9334319B2 (en) * 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
WO2013176754A1 (en) * 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
US9085618B2 (en) * 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same

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