JP2018510867A5 - - Google Patents
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- Publication number
- JP2018510867A5 JP2018510867A5 JP2017548109A JP2017548109A JP2018510867A5 JP 2018510867 A5 JP2018510867 A5 JP 2018510867A5 JP 2017548109 A JP2017548109 A JP 2017548109A JP 2017548109 A JP2017548109 A JP 2017548109A JP 2018510867 A5 JP2018510867 A5 JP 2018510867A5
- Authority
- JP
- Japan
- Prior art keywords
- wash solution
- chromatography
- protein
- matrix
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 16
- 239000012504 chromatography matrix Substances 0.000 claims 6
- 102000004169 proteins and genes Human genes 0.000 claims 6
- 108090000623 proteins and genes Proteins 0.000 claims 6
- 239000000203 mixture Substances 0.000 claims 5
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims 4
- 239000004475 Arginine Substances 0.000 claims 3
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims 3
- 238000001042 affinity chromatography Methods 0.000 claims 3
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 3
- 150000001483 arginine derivatives Chemical class 0.000 claims 3
- 210000004748 cultured cell Anatomy 0.000 claims 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 238000004587 chromatography analysis Methods 0.000 claims 2
- 210000004962 mammalian cell Anatomy 0.000 claims 2
- 229910000029 sodium carbonate Inorganic materials 0.000 claims 2
- 239000006228 supernatant Substances 0.000 claims 2
- 241000699802 Cricetulus griseus Species 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 238000005277 cation exchange chromatography Methods 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 239000013592 cell lysate Substances 0.000 claims 1
- 230000001143 conditioned effect Effects 0.000 claims 1
- 239000000356 contaminant Substances 0.000 claims 1
- 238000010828 elution Methods 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 1
- 238000004255 ion exchange chromatography Methods 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 238000012434 mixed-mode chromatography Methods 0.000 claims 1
- 210000001672 ovary Anatomy 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562132974P | 2015-03-13 | 2015-03-13 | |
| US62/132,974 | 2015-03-13 | ||
| PCT/US2016/021984 WO2016149088A1 (en) | 2015-03-13 | 2016-03-11 | Use of alkaline washes during chromatography to remove impurities |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018510867A JP2018510867A (ja) | 2018-04-19 |
| JP2018510867A5 true JP2018510867A5 (enExample) | 2019-03-22 |
| JP6807859B2 JP6807859B2 (ja) | 2021-01-06 |
Family
ID=55699796
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017548109A Active JP6807859B2 (ja) | 2015-03-13 | 2016-03-11 | クロマトグラフィーにおける不純物を取り除くためのアルカリ洗浄の使用 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US10695693B2 (enExample) |
| EP (2) | EP4194071A1 (enExample) |
| JP (1) | JP6807859B2 (enExample) |
| KR (1) | KR102490956B1 (enExample) |
| CN (1) | CN107636005B (enExample) |
| AU (2) | AU2016233557B2 (enExample) |
| BR (1) | BR112017018365A2 (enExample) |
| CA (1) | CA2978095C (enExample) |
| EA (1) | EA035635B1 (enExample) |
| IL (1) | IL254372B (enExample) |
| MX (1) | MX395730B (enExample) |
| SG (2) | SG10201908027QA (enExample) |
| WO (1) | WO2016149088A1 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10738078B2 (en) * | 2014-11-03 | 2020-08-11 | Bristol-Myers Squibb Company | Use of caprylic acid precipitation for protein purification |
| SG10201908027QA (en) | 2015-03-13 | 2019-10-30 | Bristol Myers Squibb Co | Use of alkaline washes during chromatography to remove impurities |
| KR102586771B1 (ko) | 2017-01-30 | 2023-10-11 | 리제너론 파마슈티칼스 인코포레이티드 | 크로마토그래피에서 바이오버든을 감소시키기 위한 조성물 및 방법 |
| EP3810650A1 (en) * | 2018-06-19 | 2021-04-28 | Bristol-Myers Squibb Company | Methods of purifying proteins using chromatography |
| US12152056B2 (en) * | 2018-08-17 | 2024-11-26 | Regeneron Pharmaceuticals, Inc. | Method and chromatography system for determining amount and purity of a multimeric protein |
| KR102270048B1 (ko) * | 2020-07-10 | 2021-06-28 | 주식회사 녹십자 | 바리셀라 조스터 바이러스 표면 단백질 항원의 생산 방법 |
| EP3874276B1 (en) * | 2018-10-31 | 2023-09-06 | Regeneron Pharmaceuticals, Inc. | Method and system of identifying and quantifying a protein |
| WO2020125757A1 (en) * | 2018-12-21 | 2020-06-25 | Wuxi Biologics (Shanghai) Co., Ltd. | A method for improving aggregate removal by protein a chromatography |
| WO2020150328A1 (en) * | 2019-01-16 | 2020-07-23 | Regeneron Pharmaceuticals, Inc. | Method and system of identifying and quantifying antibody fragmentation |
| CN109870428A (zh) * | 2019-01-21 | 2019-06-11 | 江苏华泰疫苗工程技术研究有限公司 | 一种基于表面等离子共振技术的抗体定量检测方法 |
| GB201904741D0 (en) * | 2019-04-04 | 2019-05-22 | Glaxosmithkline Ip Dev Ltd | Novel process |
| EP4653865A3 (en) | 2019-09-24 | 2026-02-18 | Regeneron Pharmaceuticals, Inc. | Systems and methods for chromatography use and regeneration |
| US20220396598A1 (en) * | 2019-10-14 | 2022-12-15 | Pierce Biotechnology, Inc. | Peptide purification formulations and methods |
| BR112022019076A2 (pt) * | 2020-03-27 | 2023-01-10 | HaemaLogiX Ltd | Composição e método |
| CN116348486A (zh) * | 2020-10-02 | 2023-06-27 | 伊莱利利公司 | 在蛋白质纯化过程中降低宿主细胞蛋白质含量的方法 |
| WO2025178923A1 (en) * | 2024-02-20 | 2025-08-28 | Exelixis, Inc. | Methods for protein purification |
Family Cites Families (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| US5225539A (en) | 1986-03-27 | 1993-07-06 | Medical Research Council | Recombinant altered antibodies and methods of making altered antibodies |
| US5190859A (en) * | 1987-02-26 | 1993-03-02 | Dana-Farber Cancer Institute, Inc. | Purification of LFA-3 |
| CA1326940C (en) * | 1987-02-26 | 1994-02-08 | Michael Dustin | Purification of lfa-3 |
| CA1323567C (en) * | 1987-10-05 | 1993-10-26 | Gene R. Nathans | Method for purification of antibodies |
| US5476996A (en) | 1988-06-14 | 1995-12-19 | Lidak Pharmaceuticals | Human immune system in non-human animal |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| GB8823869D0 (en) | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
| US5530101A (en) | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
| ES2087997T3 (es) | 1990-01-12 | 1996-08-01 | Cell Genesys Inc | Generacion de anticuerpos xenogenicos. |
| US6673986B1 (en) | 1990-01-12 | 2004-01-06 | Abgenix, Inc. | Generation of xenogeneic antibodies |
| US6075181A (en) | 1990-01-12 | 2000-06-13 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
| US6150584A (en) | 1990-01-12 | 2000-11-21 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
| US5427908A (en) | 1990-05-01 | 1995-06-27 | Affymax Technologies N.V. | Recombinant library screening methods |
| US6172197B1 (en) | 1991-07-10 | 2001-01-09 | Medical Research Council | Methods for producing members of specific binding pairs |
| GB9015198D0 (en) | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
| US5874299A (en) | 1990-08-29 | 1999-02-23 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
| US5770429A (en) | 1990-08-29 | 1998-06-23 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
| US5545806A (en) | 1990-08-29 | 1996-08-13 | Genpharm International, Inc. | Ransgenic non-human animals for producing heterologous antibodies |
| US5877397A (en) | 1990-08-29 | 1999-03-02 | Genpharm International Inc. | Transgenic non-human animals capable of producing heterologous antibodies of various isotypes |
| US6300129B1 (en) | 1990-08-29 | 2001-10-09 | Genpharm International | Transgenic non-human animals for producing heterologous antibodies |
| US5661016A (en) | 1990-08-29 | 1997-08-26 | Genpharm International Inc. | Transgenic non-human animals capable of producing heterologous antibodies of various isotypes |
| ES2108048T3 (es) | 1990-08-29 | 1997-12-16 | Genpharm Int | Produccion y utilizacion de animales inferiores transgenicos capaces de producir anticuerpos heterologos. |
| US5814318A (en) | 1990-08-29 | 1998-09-29 | Genpharm International Inc. | Transgenic non-human animals for producing heterologous antibodies |
| US5625126A (en) | 1990-08-29 | 1997-04-29 | Genpharm International, Inc. | Transgenic non-human animals for producing heterologous antibodies |
| US5789650A (en) | 1990-08-29 | 1998-08-04 | Genpharm International, Inc. | Transgenic non-human animals for producing heterologous antibodies |
| US5633425A (en) | 1990-08-29 | 1997-05-27 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
| US6255458B1 (en) | 1990-08-29 | 2001-07-03 | Genpharm International | High affinity human antibodies and human antibodies against digoxin |
| ATE463573T1 (de) | 1991-12-02 | 2010-04-15 | Medimmune Ltd | Herstellung von autoantikörpern auf phagenoberflächen ausgehend von antikörpersegmentbibliotheken |
| EP0746609A4 (en) | 1991-12-17 | 1997-12-17 | Genpharm Int | NON-HUMAN TRANSGENIC ANIMALS CAPABLE OF PRODUCING HETEROLOGOUS ANTIBODIES |
| EP1005870B1 (en) | 1992-11-13 | 2009-01-21 | Biogen Idec Inc. | Therapeutic application of chimeric antibodies to human B lymphocyte restricted differentiation antigen for treatment of B cell lymphoma |
| JPH08509612A (ja) | 1993-04-26 | 1996-10-15 | ジェンファーム インターナショナル インコーポレイテッド | 異種抗体を産生することができるトランスジェニック非ヒト動物 |
| US5534615A (en) | 1994-04-25 | 1996-07-09 | Genentech, Inc. | Cardiac hypertrophy factor and uses therefor |
| ES2292682T3 (es) * | 1998-05-06 | 2008-03-16 | Genentech, Inc. | Purificacion de anticuerpos mediante cromatografia de intercambio ionico. |
| NZ517202A (en) | 1999-08-24 | 2004-05-28 | Medarex Inc | Human CTLA-4 antibodies and their uses |
| JP3523245B1 (ja) | 2000-11-30 | 2004-04-26 | メダレックス，インコーポレーテッド | ヒト抗体作製用トランスジェニック染色体導入齧歯動物 |
| ATE417858T1 (de) | 2002-02-05 | 2009-01-15 | Genentech Inc | Proteinaufreinigung |
| US7763706B1 (en) * | 2003-07-17 | 2010-07-27 | Campina B.V. | Arginine/lysine-enriched peptides |
| PT1678208E (pt) * | 2003-10-27 | 2013-07-10 | Wyeth Llc | Remoção de agregados de elevado peso molecular utilizando cromatografia de hidroxiapatite |
| ES2342100T3 (es) * | 2005-04-26 | 2010-07-01 | Sandoz Ag | Ligandos de afinidad. |
| US8263750B2 (en) * | 2006-03-16 | 2012-09-11 | Amgen Inc. | Method for purifying a protein using protein-A affinity chromatography using an intermediate wash step |
| FI2061803T4 (fi) * | 2006-08-28 | 2023-03-14 | Process for the purification of fc-containing proteins | |
| CL2007002615A1 (es) | 2006-09-08 | 2008-04-18 | Wyeth Corp | Metodos para aislar o purificar un producto que comprende poner el producto enlazado en contacto con al menos una solucion de lavado que comprende arginina y luego eluir el producto; y dicho producto. |
| BRPI0817182A2 (pt) * | 2007-10-30 | 2015-03-17 | Genentech Inc | Método para purificar um anticorpo e composições |
| TWI610936B (zh) * | 2008-10-20 | 2018-01-11 | 艾伯維有限公司 | 使用蛋白質ａ親和性層析進行抗體之分離及純化 |
| CA2784278C (en) | 2009-12-18 | 2019-02-26 | Novartis Ag | Wash solution and method for affinity chromatography |
| JP2012001462A (ja) * | 2010-06-15 | 2012-01-05 | Kaneka Corp | 抗体精製用カラム |
| JP6366502B2 (ja) | 2011-06-01 | 2018-08-01 | ノバルティス アーゲー | アフィニティクロマトグラフィーのための、洗浄溶液および方法 |
| AR096713A1 (es) * | 2013-06-25 | 2016-01-27 | Cadila Healthcare Ltd | Proceso de purificación para anticuerpos monoclonales |
| US9758578B2 (en) * | 2013-12-26 | 2017-09-12 | Mitsubishi Tanabe Pharma Corporation | Human anti-IL-33 neutralizing monoclonal antibody |
| WO2016031932A1 (ja) | 2014-08-27 | 2016-03-03 | 田辺三菱製薬株式会社 | アルカリ洗浄によるＦｃ領域を有するタンパク質の製造方法 |
| SG10201908027QA (en) | 2015-03-13 | 2019-10-30 | Bristol Myers Squibb Co | Use of alkaline washes during chromatography to remove impurities |
-
2016
- 2016-03-11 SG SG10201908027Q patent/SG10201908027QA/en unknown
- 2016-03-11 CA CA2978095A patent/CA2978095C/en active Active
- 2016-03-11 AU AU2016233557A patent/AU2016233557B2/en active Active
- 2016-03-11 CN CN201680015033.0A patent/CN107636005B/zh active Active
- 2016-03-11 SG SG11201707330RA patent/SG11201707330RA/en unknown
- 2016-03-11 WO PCT/US2016/021984 patent/WO2016149088A1/en not_active Ceased
- 2016-03-11 MX MX2017011121A patent/MX395730B/es unknown
- 2016-03-11 EA EA201792012A patent/EA035635B1/ru not_active IP Right Cessation
- 2016-03-11 JP JP2017548109A patent/JP6807859B2/ja active Active
- 2016-03-11 EP EP22206303.4A patent/EP4194071A1/en active Pending
- 2016-03-11 US US15/558,033 patent/US10695693B2/en active Active
- 2016-03-11 EP EP16715383.2A patent/EP3268100A1/en not_active Withdrawn
- 2016-03-11 KR KR1020177028511A patent/KR102490956B1/ko active Active
- 2016-03-11 BR BR112017018365A patent/BR112017018365A2/pt not_active Application Discontinuation
-
2017
- 2017-09-07 IL IL254372A patent/IL254372B/en unknown
-
2020
- 2020-05-22 US US16/881,102 patent/US12157073B2/en active Active
-
2021
- 2021-08-26 AU AU2021221870A patent/AU2021221870A1/en not_active Abandoned
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