JP2014532718A5 - - Google Patents

Download PDF

Info

Publication number
JP2014532718A5
JP2014532718A5 JP2014540118A JP2014540118A JP2014532718A5 JP 2014532718 A5 JP2014532718 A5 JP 2014532718A5 JP 2014540118 A JP2014540118 A JP 2014540118A JP 2014540118 A JP2014540118 A JP 2014540118A JP 2014532718 A5 JP2014532718 A5 JP 2014532718A5
Authority
JP
Japan
Prior art keywords
polypeptide
conductivity
elution buffer
buffer
antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2014540118A
Other languages
English (en)
Japanese (ja)
Other versions
JP6356607B2 (ja
JP2014532718A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2012/063242 external-priority patent/WO2013067301A1/en
Publication of JP2014532718A publication Critical patent/JP2014532718A/ja
Publication of JP2014532718A5 publication Critical patent/JP2014532718A5/ja
Application granted granted Critical
Publication of JP6356607B2 publication Critical patent/JP6356607B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2014540118A 2011-11-02 2012-11-02 オーバーロード／溶出クロマトグラフィー Active JP6356607B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161554898P 2011-11-02 2011-11-02
US61/554,898 2011-11-02
PCT/US2012/063242 WO2013067301A1 (en) 2011-11-02 2012-11-02 Overload and elute chromatography

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2018113263A Division JP2018184405A (ja) 2011-11-02 2018-06-14 オーバーロード／溶出クロマトグラフィー

Publications (3)

Publication Number Publication Date
JP2014532718A JP2014532718A (ja) 2014-12-08
JP2014532718A5 true JP2014532718A5 (enExample) 2015-12-24
JP6356607B2 JP6356607B2 (ja) 2018-07-11

Family

ID=48192803

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2014540118A Active JP6356607B2 (ja) 2011-11-02 2012-11-02 オーバーロード／溶出クロマトグラフィー
JP2018113263A Pending JP2018184405A (ja) 2011-11-02 2018-06-14 オーバーロード／溶出クロマトグラフィー
JP2019209674A Active JP6979442B2 (ja) 2011-11-02 2019-11-20 オーバーロード／溶出クロマトグラフィー
JP2021185350A Active JP7203928B2 (ja) 2011-11-02 2021-11-15 オーバーロード／溶出クロマトグラフィー

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2018113263A Pending JP2018184405A (ja) 2011-11-02 2018-06-14 オーバーロード／溶出クロマトグラフィー
JP2019209674A Active JP6979442B2 (ja) 2011-11-02 2019-11-20 オーバーロード／溶出クロマトグラフィー
JP2021185350A Active JP7203928B2 (ja) 2011-11-02 2021-11-15 オーバーロード／溶出クロマトグラフィー

Country Status (25)

Country Link
US (3) US20140301977A1 (enExample)
EP (3) EP2773438B2 (enExample)
JP (4) JP6356607B2 (enExample)
KR (1) KR20140091721A (enExample)
CN (2) CN107163101B (enExample)
AU (1) AU2012323995B2 (enExample)
BR (1) BR112014010406A2 (enExample)
CA (1) CA2854330A1 (enExample)
DK (2) DK3257564T4 (enExample)
ES (3) ES3062963T3 (enExample)
FI (1) FI3257564T4 (enExample)
HR (2) HRP20260140T1 (enExample)
HU (2) HUE043755T2 (enExample)
IL (1) IL232146A0 (enExample)
LT (1) LT3257564T (enExample)
MX (1) MX2014005330A (enExample)
PL (3) PL3257564T5 (enExample)
PT (1) PT3257564T (enExample)
RS (1) RS58472B2 (enExample)
RU (1) RU2014121884A (enExample)
SG (1) SG11201402019TA (enExample)
SI (2) SI3257564T2 (enExample)
TR (1) TR201903707T4 (enExample)
WO (1) WO2013067301A1 (enExample)
ZA (1) ZA201402932B (enExample)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012015908A2 (en) 2010-07-30 2012-02-02 Millipore Corporation Chromatography media and method
WO2012149197A2 (en) 2011-04-27 2012-11-01 Abbott Laboratories Methods for controlling the galactosylation profile of recombinantly-expressed proteins
KR20140091721A (ko) 2011-11-02 2014-07-22 제넨테크, 인크. 오버로드 및 용리 크로마토그래피
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9150645B2 (en) 2012-04-20 2015-10-06 Abbvie, Inc. Cell culture methods to reduce acidic species
US9249182B2 (en) * 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
MX379269B (es) * 2012-10-24 2025-03-11 Genzyme Corp Elucion de biomoleculas de resinas multimodales utilizando mes y mops como modificadores de fase movil.
SG11201507230PA (en) 2013-03-12 2015-10-29 Abbvie Inc Human antibodies that bind human tnf-alpha and methods of preparing the same
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
US9951101B2 (en) 2013-12-12 2018-04-24 Emd Millipore Corporation Protein separations using an acrylamide containing filter
EP3079722B1 (en) * 2013-12-13 2021-03-24 Novo Nordisk Health Care AG Method for thioether conjugation of proteins
KR20150083689A (ko) * 2014-01-10 2015-07-20 삼성전자주식회사 항 c-Met 항체 정제 방법
EP3116891B1 (en) 2014-03-10 2020-02-12 Richter Gedeon Nyrt. Immunoglobulin purification using pre-cleaning steps
CN106460027B (zh) * 2014-03-24 2020-07-31 日东纺绩株式会社 使用阳离子性接枝聚合物的目标物分离浓缩方法
SG11201609908VA (en) * 2014-05-28 2016-12-29 Agency Science Tech & Res Virus reduction method
CA2951408A1 (en) * 2014-06-25 2015-12-30 Jhl Biotech, Inc. Methods and reagents for purification of proteins
CN104208719B (zh) * 2014-09-24 2017-05-03 北京天广实生物技术股份有限公司 一种抗体偶联药物的阳离子交换层析纯化方法
US20170298091A1 (en) 2014-12-08 2017-10-19 Emd Millipore Corporation Mixed Bed Ion Exchange Adsorber
HRP20192217T1 (hr) * 2015-03-06 2020-02-21 F. Hoffmann - La Roche Ag ULTRA-PROČIŠĆENI DsbA I DsbC TE POSTUPCI NJIHOVE IZRADE I UPORABE
CN106290591B (zh) * 2015-05-14 2019-07-26 上海医药工业研究院 一种庆大霉素C1a的检测方法
GB2539420B (en) * 2015-06-16 2021-01-13 Cytiva Sweden Ab Determination of chromatography conditions
MX388197B (es) 2015-08-21 2025-03-19 Hoffmann La Roche Método para la reducción de proteínas de células hospederas en cromatografía de afinidad.
CN107849122B (zh) * 2015-08-21 2022-01-25 豪夫迈·罗氏有限公司 用低电导率洗涤缓冲液进行亲和层析纯化
CN115598231B (zh) * 2016-08-15 2025-11-18 豪夫迈·罗氏有限公司 定量包含非离子表面活性剂和多肽的组合物中非离子表面活性剂的层析方法
GB201702856D0 (en) * 2017-02-22 2017-04-05 Ge Healthcare Bio Sciences Ab Method in continuous chromatography
US11339377B2 (en) * 2017-11-08 2022-05-24 Bluesky Immunotherapies Gmbh SO3 chromatography for use in a method for virus purification
CA3104392A1 (en) * 2018-07-04 2020-01-09 Probiogen Ag Method for purifying an enveloped virus
US12306152B2 (en) * 2018-12-21 2025-05-20 Solventum Intellectual Properties Company Method for testing a chromatography device used for ion exchange
WO2020172658A1 (en) 2019-02-24 2020-08-27 Bristol-Myers Squibb Company Methods of isolating a protein
AR119264A1 (es) 2019-06-05 2021-12-09 Genentech Inc Método para reutilización de cromatografía
CN111351876A (zh) * 2020-04-01 2020-06-30 上海中科新生命生物科技有限公司 一种抗体药中宿主细胞蛋白半绝对定量检测方法
US11379651B1 (en) * 2020-07-06 2022-07-05 Turtl Surf & Immerse Limited Methods and systems for interactive content creation
CN116194570A (zh) * 2020-09-22 2023-05-30 百时美施贵宝公司 用于产生治疗性蛋白的方法
JP7620456B2 (ja) * 2021-03-15 2025-01-23 株式会社カネカ κ鎖を含む抗体の製造方法
CA3233419A1 (en) * 2021-09-28 2023-04-06 Kashiv Biosciences, Llc An improved process for purification of protein
CA3236213A1 (en) * 2021-10-27 2023-05-04 Eugene ZURLO Compositions and methods for isolating proteins

Family Cites Families (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2413974A1 (fr) 1978-01-06 1979-08-03 David Bernard Sechoir pour feuilles imprimees par serigraphie
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4676980A (en) 1985-09-23 1987-06-30 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target specific cross-linked heteroantibodies
US5567610A (en) 1986-09-04 1996-10-22 Bioinvent International Ab Method of producing human monoclonal antibodies and kit therefor
IL85035A0 (en) 1987-01-08 1988-06-30 Int Genetic Eng Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
US5175384A (en) 1988-12-05 1992-12-29 Genpharm International Transgenic mice depleted in mature t-cells and methods for making transgenic mice
EP0402226A1 (en) 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Transformation vectors for yeast yarrowia
DE3920358A1 (de) 1989-06-22 1991-01-17 Behringwerke Ag Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung
EP0739904A1 (en) 1989-06-29 1996-10-30 Medarex, Inc. Bispecific reagents for aids therapy
US5208020A (en) 1989-10-25 1993-05-04 Immunogen Inc. Cytotoxic agents comprising maytansinoids and their therapeutic use
US5229275A (en) 1990-04-26 1993-07-20 Akzo N.V. In-vitro method for producing antigen-specific human monoclonal antibodies
AU8295491A (en) 1990-06-29 1992-01-23 Biosource Technologies Incorporated Melanin production by transformed microorganisms
US5571894A (en) 1991-02-05 1996-11-05 Ciba-Geigy Corporation Recombinant antibodies specific for a growth factor receptor
WO1992020373A1 (en) 1991-05-14 1992-11-26 Repligen Corporation Heteroconjugate antibodies for treatment of hiv infection
EP0590058B1 (en) 1991-06-14 2003-11-26 Genentech, Inc. HUMANIZED Heregulin ANTIBODy
IE922437A1 (en) 1991-07-25 1993-01-27 Idec Pharma Corp Recombinant antibodies for human therapy
ES2136092T3 (es) 1991-09-23 1999-11-16 Medical Res Council Procedimientos para la produccion de anticuerpos humanizados.
FI941572L (fi) 1991-10-07 1994-05-27 Oncologix Inc Anti-erbB-2-monoklonaalisten vasta-aineiden yhdistelmä ja käyttömenetelmä
WO1993008829A1 (en) 1991-11-04 1993-05-13 The Regents Of The University Of California Compositions that mediate killing of hiv-infected cells
DE69233528T2 (de) 1991-11-25 2006-03-16 Enzon, Inc. Verfahren zur Herstellung von multivalenten antigenbindenden Proteinen
CA2372813A1 (en) 1992-02-06 1993-08-19 L.L. Houston Biosynthetic binding protein for cancer marker
EP0626012B1 (en) 1992-02-11 2003-07-09 Cell Genesys, Inc. Homogenotization of gene-targeting events
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
DE69308573T2 (de) 1992-08-17 1997-08-07 Genentech Inc Bispezifische immunoadhesine
ATE139900T1 (de) 1992-11-13 1996-07-15 Idec Pharma Corp Therapeutische verwendung von chimerischen und markierten antikörper gegen menschlichen b lymphozyt beschränkter differenzierung antigen für die behandlung von b-zell-lymphoma
CA2106612C (en) * 1993-09-21 2001-02-06 Diana Pliura Displacement chromatography process
US5534615A (en) 1994-04-25 1996-07-09 Genentech, Inc. Cardiac hypertrophy factor and uses therefor
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
US5712374A (en) 1995-06-07 1998-01-27 American Cyanamid Company Method for the preparation of substantiallly monomeric calicheamicin derivative/carrier conjugates
NZ506214A (en) * 1996-09-13 2002-11-26 Transkaryotic Therapies Inc Therapy for alpha-galactosidase A deficiency
ES2293661T3 (es) * 1996-11-27 2008-03-16 Genentech, Inc. Purificacion por afinidad de polipeptido en una matriz de proteinas a.
ATE370961T1 (de) * 1998-05-06 2007-09-15 Genentech Inc Reinigung von antikörpern durch ionenaustauschchromatographie
IL127127A0 (en) 1998-11-18 1999-09-22 Peptor Ltd Small functional units of antibody heavy chain variable regions
ES2376454T3 (es) 2000-12-22 2012-03-14 Grad, Carole, Legal Representative Of Howard, Kaplan Librer�?as de expresión en fago de fragmentos vh humanos.
JP4259868B2 (ja) * 2001-02-01 2009-04-30 バイオジェン・アイデック・エムエイ・インコーポレイテッド ニューブラスチンのポリマー結合体および同様の使用方法。
JP2005289809A (ja) 2001-10-24 2005-10-20 Vlaams Interuniversitair Inst Voor Biotechnologie Vzw (Vib Vzw) 突然変異重鎖抗体
US20070261962A1 (en) * 2002-04-02 2007-11-15 Ryszard Gajek Separation Systems with Charge Mosaic Membrane
EP1552878A4 (en) * 2002-10-18 2006-03-22 Asahi Kasei Pharma Corp MICROPOR SE HYDROPHILIC MEMBRANE
JP5087274B2 (ja) 2003-06-30 2012-12-05 ドマンティス リミテッド ポリペプチド
CA2577133A1 (en) 2004-08-19 2006-03-23 Genentech, Inc. Polypeptide variants with altered effector function
TR201808537T4 (tr) 2004-09-23 2018-07-23 Genentech Inc Sistein değiştirilmiş antikorlar ve konjugatlar.
BRPI0608584B8 (pt) * 2005-03-11 2021-05-25 Wyeth Corp métodos de recuperar um produto purificado
DE112006001012B4 (de) 2005-04-22 2017-05-18 Waters Technologies Corp. (N.D.Ges.D. Staates Delaware) Verfahren für eine auf Massenspektrometrie gerichtete Aufbereitung von Biopolymeren
KR20090058532A (ko) * 2006-08-28 2009-06-09 아레스 트레이딩 에스.에이. Ｆｃ­융합 단백질을 정제하는 방법
CN101535335B (zh) * 2006-08-28 2013-10-02 阿雷斯贸易股份有限公司 含Fc-蛋白的纯化方法
RU2009148891A (ru) 2007-06-01 2011-07-20 Ф.Хоффманн-Ля Рош Аг (Ch) Очистка иммуноглобулина
DE102009005479A1 (de) * 2009-01-21 2010-07-22 Sartorius Stedim Biotech Gmbh Vorrichtung und Verfahren für die Stofftrennung
JP5614936B2 (ja) * 2009-02-19 2014-10-29 旭化成ケミカルズ株式会社 アニオン交換基が固定された多孔膜を用いた核酸の精製方法
US8536316B2 (en) * 2009-08-07 2013-09-17 Emd Millipore Corporation Methods for purifying a target protein from one or more impurities in a sample
WO2011035282A1 (en) * 2009-09-21 2011-03-24 Ge Healthcare Bio-Sciences Ab Dual capture separation
CN102094034A (zh) * 2009-12-09 2011-06-15 泰州新生源生物医药有限公司 一种重组人Fc融合长效干扰素的纯化工艺
WO2012030512A1 (en) * 2010-09-03 2012-03-08 Percivia Llc. Flow-through protein purification process
JP2014503495A (ja) * 2010-11-15 2014-02-13 バイオジェン アイデック インコーポレイテッド 過負荷結合および溶出クロマトグラフィによる選択生成物アイソフォームの富化および濃縮
KR20140091721A (ko) 2011-11-02 2014-07-22 제넨테크, 인크. 오버로드 및 용리 크로마토그래피

Similar Documents

Publication Publication Date Title
RU2014121884A (ru) Хроматография перегрузки и элюирования
RU2014121886A (ru) Устройство для обработки курительного изделия и картридж для него
US9957318B2 (en) Protein purification methods to reduce acidic species
EP3912987B9 (en) Purification platform for bispecific antibodies
US7691980B2 (en) Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
JP2016530533A5 (enExample)
JP5567691B2 (ja) Ｆｃ含有タンパク質を精製するためのクロマトグラフィー法
JP4852602B2 (ja) 抗体の精製法
JP2013530156A5 (enExample)
US20120077961A1 (en) Enhanced purification of antibodies and antibody fragments by apatite chromatography
WO2013176754A9 (en) Novel purification of antibodies using hydrophobic interaction chromatography
CA2903496A1 (en) Methods of increasing protein purity using protein a based chromatography
CN108350027A (zh) 用于改进的蛋白质分离的相反的pH-盐梯度
CN108350026A (zh) 离子交换色谱法中改进的蛋白质分离
CN112771379A (zh) 用于测定多聚体蛋白质的量和纯度的方法和色谱系统
EP4026596A1 (en) Removal of leaked affinity purification ligand
CN104136121A (zh) 减少离子交换色谱中的pH漂移
KR101700580B1 (ko) 양이온 교환 크로마토그래피를 이용한 항체의 아형 분리 방법
CN106414495A (zh) 抗体处理方法
JP2014523405A5 (enExample)
Ishihara et al. Evaluation of new affinity chromatography resins for polyclonal, oligoclonal and monoclonal antibody pharmaceuticals
CN117126227A (zh) 表面活性剂在疏水层析纯化蛋白中提高回收率的应用
KR20240154657A (ko) 임상 인간 igg 제품의 친화도 크로마토그래피 생산
HK1174044B (en) Chromatographic method for purifying fc-containing proteins