JP2014523405A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2014523405A5 JP2014523405A5 JP2014508565A JP2014508565A JP2014523405A5 JP 2014523405 A5 JP2014523405 A5 JP 2014523405A5 JP 2014508565 A JP2014508565 A JP 2014508565A JP 2014508565 A JP2014508565 A JP 2014508565A JP 2014523405 A5 JP2014523405 A5 JP 2014523405A5
- Authority
- JP
- Japan
- Prior art keywords
- ligand
- sample
- target molecule
- cation exchange
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003446 ligand Substances 0.000 claims description 57
- 238000000034 method Methods 0.000 claims description 51
- 239000000523 sample Substances 0.000 claims description 26
- 238000005341 cation exchange Methods 0.000 claims description 23
- 238000004113 cell culture Methods 0.000 claims description 21
- 239000003795 chemical substances by application Substances 0.000 claims description 14
- 238000005349 anion exchange Methods 0.000 claims description 12
- 102000004169 proteins and genes Human genes 0.000 claims description 12
- 108090000623 proteins and genes Proteins 0.000 claims description 12
- 239000012472 biological sample Substances 0.000 claims description 9
- 238000000746 purification Methods 0.000 claims description 8
- 210000004027 cell Anatomy 0.000 claims description 7
- 239000011324 bead Substances 0.000 claims description 6
- 230000002209 hydrophobic effect Effects 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 150000007523 nucleic acids Chemical class 0.000 claims description 4
- 102000039446 nucleic acids Human genes 0.000 claims description 4
- 108020004707 nucleic acids Proteins 0.000 claims description 4
- 229920000642 polymer Polymers 0.000 claims description 4
- 239000012228 culture supernatant Substances 0.000 claims description 3
- 125000000542 sulfonic acid group Chemical group 0.000 claims description 3
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims description 2
- 241000238631 Hexapoda Species 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical group OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 2
- 238000004220 aggregation Methods 0.000 claims description 2
- 230000002776 aggregation Effects 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 claims description 2
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 2
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims description 2
- 150000001735 carboxylic acids Chemical group 0.000 claims description 2
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims description 2
- 238000004587 chromatography analysis Methods 0.000 claims description 2
- 229960000633 dextran sulfate Drugs 0.000 claims description 2
- 210000004962 mammalian cell Anatomy 0.000 claims description 2
- 239000013612 plasmid Substances 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 210000005253 yeast cell Anatomy 0.000 claims description 2
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/096,699 | 2011-04-28 | ||
| US13/096,699 US8911992B2 (en) | 2011-04-28 | 2011-04-28 | DNA removal in target molecule purification |
| PCT/US2012/035272 WO2012149200A1 (en) | 2011-04-28 | 2012-04-26 | Dna removal in target molecule purification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014523405A JP2014523405A (ja) | 2014-09-11 |
| JP2014523405A5 true JP2014523405A5 (enExample) | 2015-05-28 |
| JP6068442B2 JP6068442B2 (ja) | 2017-01-25 |
Family
ID=47068181
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014508565A Expired - Fee Related JP6068442B2 (ja) | 2011-04-28 | 2012-04-26 | 標的分子精製におけるｄｎａ除去法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US8911992B2 (enExample) |
| EP (1) | EP2702065B1 (enExample) |
| JP (1) | JP6068442B2 (enExample) |
| CA (1) | CA2832341C (enExample) |
| WO (1) | WO2012149200A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104470606B (zh) | 2012-05-31 | 2017-03-22 | 新加坡科技研究局 | 用带负电荷颗粒对多核苷酸的色谱纯化 |
| US20180105555A1 (en) * | 2015-03-20 | 2018-04-19 | Bristol-Myers Squibb Company | Use of dextran for protein purification |
| WO2020247738A1 (en) * | 2019-06-07 | 2020-12-10 | Dyne Therapeutics, Inc. | Methods of preparing protein-oligonucleotide complexes |
| WO2022038190A1 (en) * | 2020-08-18 | 2022-02-24 | Bia Separations D.O.O. | Metal-affinity extraction of host cell dna |
| EP4430183A4 (en) * | 2021-11-11 | 2025-10-08 | Promega Corp | CATION EXCHANGE FOR SPERM DNA PURIFICATION |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6504021B2 (en) | 2000-07-05 | 2003-01-07 | Edge Biosystems, Inc. | Ion exchange method for DNA purification |
| US20100056762A1 (en) * | 2001-05-11 | 2010-03-04 | Old Lloyd J | Specific binding proteins and uses thereof |
| WO2003059935A2 (en) * | 2001-12-21 | 2003-07-24 | Immunex Corporation | Methods for purifying protein |
| EP2738178A1 (en) | 2006-04-05 | 2014-06-04 | AbbVie Biotechnology Ltd | Antibody purification |
| MX2009012786A (es) * | 2007-06-01 | 2009-12-10 | Hoffmann La Roche | Purificacion de inmunoglobulina. |
| EP2287174B8 (en) * | 2008-01-18 | 2016-12-07 | Bio-Rad Laboratories, Inc. | Enhanced purification of antibody fragments by apatite chromatography |
| EP2307442A4 (en) * | 2008-06-24 | 2012-05-30 | Reddys Lab Ltd Dr | PURIFICATION OF MODIFIED CYTOKINES |
| JP5463492B2 (ja) * | 2008-10-08 | 2014-04-09 | 島根県 | 微生物細胞からのプラスミドｄｎａ抽出法 |
-
2011
- 2011-04-28 US US13/096,699 patent/US8911992B2/en not_active Expired - Fee Related
-
2012
- 2012-04-26 EP EP12776009.8A patent/EP2702065B1/en not_active Not-in-force
- 2012-04-26 WO PCT/US2012/035272 patent/WO2012149200A1/en not_active Ceased
- 2012-04-26 JP JP2014508565A patent/JP6068442B2/ja not_active Expired - Fee Related
- 2012-04-26 CA CA2832341A patent/CA2832341C/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6410734B2 (ja) | タンパク質調製物の凝集体含有量を低減する方法 | |
| US8093364B2 (en) | Enhanced purification of antibodies and antibody fragments by apatite chromatography | |
| JP6385374B2 (ja) | タンパク質製剤からエンドトキシンを除去するための物質および方法 | |
| Pedersen et al. | Specific and generic isolation of extracellular vesicles with magnetic beads | |
| US20150275269A1 (en) | Method for purifying nucleic acid and kit | |
| Dhadge et al. | Magnetic aqueous two phase fishing: a hybrid process technology for antibody purification | |
| JP6268167B2 (ja) | タンパク質製剤中の凝集物含量を低減するために混合型多官能表面を使用する方法 | |
| JP2016509069A (ja) | 非イオン性有機ポリマー存在下、高導電率でのタンパク質精製 | |
| JP2014523405A5 (enExample) | ||
| Mattiasson | Cryogels for biotechnological applications | |
| JP6320429B2 (ja) | 負帯電粒子を用いるウイルス調製物のクロマトグラフィー精製 | |
| CN104136121B (zh) | 减少离子交换色谱中的pH漂移 | |
| JP6068442B2 (ja) | 標的分子精製におけるｄｎａ除去法 | |
| JP2016529908A5 (enExample) | ||
| Cerff et al. | In situ magnetic separation of antibody fragments from Escherichia coli in complex media | |
| Rosti et al. | Recovery of microquantities of human epidermal growth factor from Escherichia coli Homogenate and Pichia pastoris culture medium using expanded bed adsorption | |
| WO2013121216A1 (en) | High efficiency cell capture | |
| JP6403790B2 (ja) | タンパク質調製物の凝集体含有量をアリールアニオン処理によって減少させる方法 | |
| WO2015099550A1 (en) | Magnetic liquid-liquid extraction for purification and partitioning of substances | |
| JP2016513245A (ja) | タンパク質製剤の凝集体含有量を減少させる混合多官能金属親和性表面 | |
| Fischer et al. | Nanoparticle mediated protein separation in aqueous micellar two-phase systems | |
| Liangsupree et al. | Immunoaffinity Chromatography | |
| JP2024093998A (ja) | 非特異反応を抑制可能な抗体溶液 | |
| JP2024046375A (ja) | 非特異反応を抑制可能な抗体溶液 |