CA2832341C - Separation of genomic dna from a target molecule using cation exchange - Google Patents
Separation of genomic dna from a target molecule using cation exchange Download PDFInfo
- Publication number
- CA2832341C CA2832341C CA2832341A CA2832341A CA2832341C CA 2832341 C CA2832341 C CA 2832341C CA 2832341 A CA2832341 A CA 2832341A CA 2832341 A CA2832341 A CA 2832341A CA 2832341 C CA2832341 C CA 2832341C
- Authority
- CA
- Canada
- Prior art keywords
- ligand
- sample
- cation exchange
- target molecule
- exchange ligand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/096,699 US8911992B2 (en) | 2011-04-28 | 2011-04-28 | DNA removal in target molecule purification |
| US13/096,699 | 2011-04-28 | ||
| PCT/US2012/035272 WO2012149200A1 (en) | 2011-04-28 | 2012-04-26 | Dna removal in target molecule purification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2832341A1 CA2832341A1 (en) | 2012-11-01 |
| CA2832341C true CA2832341C (en) | 2019-10-22 |
Family
ID=47068181
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2832341A Expired - Fee Related CA2832341C (en) | 2011-04-28 | 2012-04-26 | Separation of genomic dna from a target molecule using cation exchange |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US8911992B2 (enExample) |
| EP (1) | EP2702065B1 (enExample) |
| JP (1) | JP6068442B2 (enExample) |
| CA (1) | CA2832341C (enExample) |
| WO (1) | WO2012149200A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104470606B (zh) | 2012-05-31 | 2017-03-22 | 新加坡科技研究局 | 用带负电荷颗粒对多核苷酸的色谱纯化 |
| US20180105555A1 (en) * | 2015-03-20 | 2018-04-19 | Bristol-Myers Squibb Company | Use of dextran for protein purification |
| CN114025805B (zh) * | 2019-06-07 | 2025-01-24 | 达因疗法公司 | 制备蛋白质-寡核苷酸复合物的方法 |
| CN116234619B (zh) * | 2020-08-18 | 2026-07-21 | 赛多利斯比亚分离有限责任公司 | 宿主细胞dna的金属亲和提取 |
| US20230142197A1 (en) * | 2021-11-11 | 2023-05-11 | Promega Corporation | Cation exchange for sperm-associated dna purification |
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|---|---|---|---|---|
| US6504021B2 (en) | 2000-07-05 | 2003-01-07 | Edge Biosystems, Inc. | Ion exchange method for DNA purification |
| US20100056762A1 (en) * | 2001-05-11 | 2010-03-04 | Old Lloyd J | Specific binding proteins and uses thereof |
| JP4363986B2 (ja) * | 2001-12-21 | 2009-11-11 | イミュネックス・コーポレーション | タンパク質の精製方法 |
| CN102391358B (zh) | 2006-04-05 | 2016-08-10 | 艾伯维生物技术有限公司 | 抗体纯化 |
| RU2009148891A (ru) * | 2007-06-01 | 2011-07-20 | Ф.Хоффманн-Ля Рош Аг (Ch) | Очистка иммуноглобулина |
| US8093364B2 (en) * | 2008-01-18 | 2012-01-10 | Bio-Rad Laboratories, Inc. | Enhanced purification of antibodies and antibody fragments by apatite chromatography |
| EP2307442A4 (en) * | 2008-06-24 | 2012-05-30 | Reddys Lab Ltd Dr | PURIFICATION OF MODIFIED CYTOKINES |
| JP5463492B2 (ja) * | 2008-10-08 | 2014-04-09 | 島根県 | 微生物細胞からのプラスミドｄｎａ抽出法 |
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| EP2702065A4 (en) | 2014-12-24 |
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| US20120276623A1 (en) | 2012-11-01 |
| JP2014523405A (ja) | 2014-09-11 |
| CA2832341A1 (en) | 2012-11-01 |
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