JP2017521389A5 - - Google Patents
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- Publication number
- JP2017521389A5 JP2017521389A5 JP2016572703A JP2016572703A JP2017521389A5 JP 2017521389 A5 JP2017521389 A5 JP 2017521389A5 JP 2016572703 A JP2016572703 A JP 2016572703A JP 2016572703 A JP2016572703 A JP 2016572703A JP 2017521389 A5 JP2017521389 A5 JP 2017521389A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- tnfr
- fusion protein
- chromatography column
- mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004169 proteins and genes Human genes 0.000 claims 45
- 108090000623 proteins and genes Proteins 0.000 claims 45
- 108091006020 Fc-tagged proteins Proteins 0.000 claims 37
- 108060008683 Tumor Necrosis Factor Receptor Proteins 0.000 claims 37
- 102000003298 tumor necrosis factor receptor Human genes 0.000 claims 37
- 239000000203 mixture Substances 0.000 claims 34
- 239000012535 impurity Substances 0.000 claims 16
- 238000000034 method Methods 0.000 claims 16
- 108091005804 Peptidases Proteins 0.000 claims 12
- 238000012434 mixed-mode chromatography Methods 0.000 claims 10
- 238000001042 affinity chromatography Methods 0.000 claims 9
- 102000035195 Peptidases Human genes 0.000 claims 8
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 5
- 239000004365 Protease Substances 0.000 claims 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 4
- 238000004113 cell culture Methods 0.000 claims 4
- 230000002797 proteolythic effect Effects 0.000 claims 4
- 238000005571 anion exchange chromatography Methods 0.000 claims 3
- 230000000694 effects Effects 0.000 claims 3
- 238000007804 gelatin zymography Methods 0.000 claims 3
- 239000000872 buffer Substances 0.000 claims 2
- 239000006143 cell culture medium Substances 0.000 claims 2
- 238000005406 washing Methods 0.000 claims 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 claims 1
- 108010008165 Etanercept Proteins 0.000 claims 1
- 239000007995 HEPES buffer Substances 0.000 claims 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims 1
- 239000007983 Tris buffer Substances 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 239000012930 cell culture fluid Substances 0.000 claims 1
- 238000011210 chromatographic step Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 238000010828 elution Methods 0.000 claims 1
- 239000012149 elution buffer Substances 0.000 claims 1
- 229960000403 etanercept Drugs 0.000 claims 1
- 230000002209 hydrophobic effect Effects 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- CIJQGPVMMRXSQW-UHFFFAOYSA-M sodium;2-aminoacetic acid;hydroxide Chemical compound O.[Na+].NCC([O-])=O CIJQGPVMMRXSQW-UHFFFAOYSA-M 0.000 claims 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 1
- 239000011534 wash buffer Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN1919MU2014 | 2014-06-13 | ||
| IN1919/MUM/2014 | 2014-06-13 | ||
| PCT/IB2015/054494 WO2015189832A1 (en) | 2014-06-13 | 2015-06-13 | Process for the purification of tnfr:fc fusion protein |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017521389A JP2017521389A (ja) | 2017-08-03 |
| JP2017521389A5 true JP2017521389A5 (enExample) | 2018-07-19 |
| JP6747985B2 JP6747985B2 (ja) | 2020-08-26 |
Family
ID=53524922
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016572703A Expired - Fee Related JP6747985B2 (ja) | 2014-06-13 | 2015-06-13 | ＴＮＦＲ：Ｆｃ融合タンパク質の精製方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US10556942B2 (enExample) |
| EP (1) | EP3155009A1 (enExample) |
| JP (1) | JP6747985B2 (enExample) |
| CN (1) | CN106536565A (enExample) |
| AU (1) | AU2015273049B2 (enExample) |
| BR (1) | BR112016029157A8 (enExample) |
| CA (1) | CA2951766A1 (enExample) |
| MA (1) | MA40232A (enExample) |
| MX (1) | MX2016016318A (enExample) |
| PH (1) | PH12016502482A1 (enExample) |
| RU (1) | RU2698654C2 (enExample) |
| WO (1) | WO2015189832A1 (enExample) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| KR20170138426A (ko) * | 2015-03-13 | 2017-12-15 | 삼성바이오에피스 주식회사 | 항-tnf-알파 폴리펩티드 조성물 및 그 용도 |
| WO2017194592A1 (en) | 2016-05-11 | 2017-11-16 | Ge Healthcare Bioprocess R&D Ab | Method of storing a separation matrix |
| US10889615B2 (en) | 2016-05-11 | 2021-01-12 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| US11753438B2 (en) | 2016-05-11 | 2023-09-12 | Cytiva Bioprocess R&D Ab | Method of cleaning and/or sanitizing a separation matrix |
| US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| CN109071613A (zh) | 2016-05-11 | 2018-12-21 | 通用电气医疗集团生物工艺研发股份公司 | 分离基质 |
| JP7031934B2 (ja) | 2016-05-11 | 2022-03-08 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックス |
| US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
| US12448411B2 (en) | 2016-09-30 | 2025-10-21 | Cytiva Bioprocess R&D Ab | Separation method |
| EP3668985B1 (en) * | 2017-08-17 | 2021-06-16 | Just-Evotec Biologics, Inc. | Method of purifying glycosylated protein from host cell galectins and other contaminants |
| EP3676281A1 (en) * | 2017-08-30 | 2020-07-08 | Ares Trading S.A. | Method for purifying proteins |
| US11952399B2 (en) * | 2018-12-18 | 2024-04-09 | Amgen Inc. | Methods for purifying proteins |
| JP2022523063A (ja) * | 2019-01-30 | 2022-04-21 | アムジエン・インコーポレーテツド | アフリベルセプトの属性、並びにその特性決定及び修飾方法 |
| KR102286892B1 (ko) * | 2019-07-08 | 2021-08-06 | 삼천당제약주식회사 | 안과용 단백질 제제의 정제방법 |
| CN112876567A (zh) * | 2019-11-29 | 2021-06-01 | 广东菲鹏制药股份有限公司 | Fc融合蛋白及其纯化方法 |
| US20230357315A1 (en) * | 2020-03-11 | 2023-11-09 | Dr. Reddy's Laboratories Limited | METHOD OF PURIFYING AN Fc-FUSION PROTEIN |
| CN113563469A (zh) * | 2020-04-28 | 2021-10-29 | 江苏中新医药有限公司 | 高回收率纯化阿达木单抗的方法 |
| WO2022234412A1 (en) * | 2021-05-03 | 2022-11-10 | Lupin Limited | A process for purification of fc-fusion proteins |
| WO2023053032A1 (en) * | 2021-09-28 | 2023-04-06 | Kashiv Biosciences, Llc | An improved process for purification of fusion protein |
| WO2023053030A1 (en) * | 2021-09-28 | 2023-04-06 | Kashiv Biosciences, Llc | An improved process for purification of protein |
| AU2022358612A1 (en) * | 2021-09-28 | 2024-04-11 | Kashiv Biosciences, Llc | An improved process of purification of fusion protein |
| MX2024004764A (es) * | 2021-10-19 | 2024-07-04 | Alteogen Inc | Procedimiento para purificar una proteína de fusión que tiene un dominio fc de igg. |
| EP4435107A4 (en) * | 2021-11-29 | 2025-12-31 | Tosoh Corp | ANTIBODY ISOLATION METHOD |
| CN119060168A (zh) * | 2024-09-26 | 2024-12-03 | 广东丸美生物技术股份有限公司 | 一种具有低电导率的重组胶原蛋白溶液及其制备方法和应用 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2021284C1 (ru) * | 1991-05-30 | 1994-10-15 | Всесоюзный научно-исследовательский институт технологии кровезаменителей и гормональных препаратов | Способ очистки клинических фракций декстрана |
| US7294481B1 (en) | 1999-01-05 | 2007-11-13 | Immunex Corporation | Method for producing recombinant proteins |
| MXPA04008215A (es) | 2002-02-27 | 2004-11-26 | Immunex Corp | Formulacion polipeptidica. |
| AU2004215653B2 (en) | 2003-02-28 | 2011-03-17 | Lonza Biologics Plc. | Antibody purification by protein A and ion exchange chromatography |
| US8263750B2 (en) * | 2006-03-16 | 2012-09-11 | Amgen Inc. | Method for purifying a protein using protein-A affinity chromatography using an intermediate wash step |
| JP2010501622A (ja) * | 2006-08-28 | 2010-01-21 | アレス トレーディング ソシエテ アノニム | Ｆｃ−融合タンパク質の精製法 |
| ES2755386T5 (es) * | 2006-08-28 | 2023-04-05 | Ares Trading Sa | Proceso para la purificación de proteínas que contienen fragmentos Fc |
| US20100256337A1 (en) | 2007-10-22 | 2010-10-07 | Merck Serono Sa | Method for purifying an fc-containing protein |
| WO2009111347A1 (en) * | 2008-02-29 | 2009-09-11 | Biogen Idec Ma Inc. | Purified immunoglobulin fusion proteins and methods of their purification |
| KR20110079693A (ko) * | 2008-10-29 | 2011-07-07 | 와이어쓰 엘엘씨 | 단일 도메인 항원 결합 분자의 정제 방법 |
| EP2526114B1 (de) * | 2010-01-22 | 2014-06-18 | Boehringer Ingelheim International GmbH | Chromatographisches verfahren zur aufreinigung von fc enthaltenden proteinen |
| WO2012176158A1 (en) * | 2011-06-24 | 2012-12-27 | Dr. Reddy's Laboratories Limited | Purification of chimeric protein |
| AU2012282960A1 (en) * | 2011-07-08 | 2014-01-16 | Merck Sharp & Dohme Corp. | Method for purifying Fc-fusion protein |
| JP6223338B2 (ja) * | 2011-08-17 | 2017-11-01 | アレス トレーディング ソシエテ アノニム | 活性型ＴＮＦＲ−Ｆｃ融合タンパク質を製造する方法 |
| US9249182B2 (en) | 2012-05-24 | 2016-02-02 | Abbvie, Inc. | Purification of antibodies using hydrophobic interaction chromatography |
| SG11201501460RA (en) * | 2012-09-11 | 2015-04-29 | Coherus Biosciences Inc | Correctly folded etanercept in high purity and excellent yield |
| CN102911250B (zh) * | 2012-09-29 | 2014-04-16 | 浙江海正药业股份有限公司 | 酸性重组蛋白药物的纯化方法 |
| EP2919812A4 (en) * | 2012-11-19 | 2016-05-18 | Merck Sharp & Dohme | Liquid formulations for TNFR: FC fusion proteins |
| ES2964601T3 (es) * | 2013-03-14 | 2024-04-08 | Amgen Inc | Eliminación de ligando de purificación por afinidad filtrado |
| US8946395B1 (en) * | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
-
2015
- 2015-06-13 EP EP15734731.1A patent/EP3155009A1/en not_active Withdrawn
- 2015-06-13 CN CN201580038801.XA patent/CN106536565A/zh active Pending
- 2015-06-13 BR BR112016029157A patent/BR112016029157A8/pt not_active Application Discontinuation
- 2015-06-13 CA CA2951766A patent/CA2951766A1/en not_active Abandoned
- 2015-06-13 WO PCT/IB2015/054494 patent/WO2015189832A1/en not_active Ceased
- 2015-06-13 MX MX2016016318A patent/MX2016016318A/es unknown
- 2015-06-13 JP JP2016572703A patent/JP6747985B2/ja not_active Expired - Fee Related
- 2015-06-13 US US15/318,489 patent/US10556942B2/en active Active
- 2015-06-13 AU AU2015273049A patent/AU2015273049B2/en not_active Ceased
- 2015-06-13 RU RU2017100005A patent/RU2698654C2/ru active
- 2015-06-13 MA MA040232A patent/MA40232A/fr unknown
-
2016
- 2016-12-13 PH PH12016502482A patent/PH12016502482A1/en unknown
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