RU2698654C2 - Способ очистки белка слияния - Google Patents
Способ очистки белка слияния Download PDFInfo
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- RU2698654C2 RU2698654C2 RU2017100005A RU2017100005A RU2698654C2 RU 2698654 C2 RU2698654 C2 RU 2698654C2 RU 2017100005 A RU2017100005 A RU 2017100005A RU 2017100005 A RU2017100005 A RU 2017100005A RU 2698654 C2 RU2698654 C2 RU 2698654C2
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- protein
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- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/12—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the preparation of the feed
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- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
- B01D15/203—Equilibration or regeneration
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- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/30—Partition chromatography
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- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
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- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
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- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3847—Multimodal interactions
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
- C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
Landscapes
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- Enzymes And Modification Thereof (AREA)
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| PCT/IB2015/054494 WO2015189832A1 (en) | 2014-06-13 | 2015-06-13 | Process for the purification of tnfr:fc fusion protein |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2849383C2 (ru) * | 2021-10-19 | 2025-10-24 | Альтеоджен, Инк | Способ очистки слитого белка, имеющего Fc-домен IgG |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| KR20170138426A (ko) * | 2015-03-13 | 2017-12-15 | 삼성바이오에피스 주식회사 | 항-tnf-알파 폴리펩티드 조성물 및 그 용도 |
| WO2017194592A1 (en) | 2016-05-11 | 2017-11-16 | Ge Healthcare Bioprocess R&D Ab | Method of storing a separation matrix |
| US10889615B2 (en) | 2016-05-11 | 2021-01-12 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| US11753438B2 (en) | 2016-05-11 | 2023-09-12 | Cytiva Bioprocess R&D Ab | Method of cleaning and/or sanitizing a separation matrix |
| US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| CN109071613A (zh) | 2016-05-11 | 2018-12-21 | 通用电气医疗集团生物工艺研发股份公司 | 分离基质 |
| JP7031934B2 (ja) | 2016-05-11 | 2022-03-08 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックス |
| US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
| US12448411B2 (en) | 2016-09-30 | 2025-10-21 | Cytiva Bioprocess R&D Ab | Separation method |
| EP3668985B1 (en) * | 2017-08-17 | 2021-06-16 | Just-Evotec Biologics, Inc. | Method of purifying glycosylated protein from host cell galectins and other contaminants |
| EP3676281A1 (en) * | 2017-08-30 | 2020-07-08 | Ares Trading S.A. | Method for purifying proteins |
| US11952399B2 (en) * | 2018-12-18 | 2024-04-09 | Amgen Inc. | Methods for purifying proteins |
| JP2022523063A (ja) * | 2019-01-30 | 2022-04-21 | アムジエン・インコーポレーテツド | アフリベルセプトの属性、並びにその特性決定及び修飾方法 |
| KR102286892B1 (ko) * | 2019-07-08 | 2021-08-06 | 삼천당제약주식회사 | 안과용 단백질 제제의 정제방법 |
| CN112876567A (zh) * | 2019-11-29 | 2021-06-01 | 广东菲鹏制药股份有限公司 | Fc融合蛋白及其纯化方法 |
| US20230357315A1 (en) * | 2020-03-11 | 2023-11-09 | Dr. Reddy's Laboratories Limited | METHOD OF PURIFYING AN Fc-FUSION PROTEIN |
| CN113563469A (zh) * | 2020-04-28 | 2021-10-29 | 江苏中新医药有限公司 | 高回收率纯化阿达木单抗的方法 |
| WO2022234412A1 (en) * | 2021-05-03 | 2022-11-10 | Lupin Limited | A process for purification of fc-fusion proteins |
| WO2023053032A1 (en) * | 2021-09-28 | 2023-04-06 | Kashiv Biosciences, Llc | An improved process for purification of fusion protein |
| WO2023053030A1 (en) * | 2021-09-28 | 2023-04-06 | Kashiv Biosciences, Llc | An improved process for purification of protein |
| AU2022358612A1 (en) * | 2021-09-28 | 2024-04-11 | Kashiv Biosciences, Llc | An improved process of purification of fusion protein |
| MX2024004764A (es) * | 2021-10-19 | 2024-07-04 | Alteogen Inc | Procedimiento para purificar una proteína de fusión que tiene un dominio fc de igg. |
| EP4435107A4 (en) * | 2021-11-29 | 2025-12-31 | Tosoh Corp | ANTIBODY ISOLATION METHOD |
| CN119060168A (zh) * | 2024-09-26 | 2024-12-03 | 广东丸美生物技术股份有限公司 | 一种具有低电导率的重组胶原蛋白溶液及其制备方法和应用 |
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| RU2021284C1 (ru) * | 1991-05-30 | 1994-10-15 | Всесоюзный научно-исследовательский институт технологии кровезаменителей и гормональных препаратов | Способ очистки клинических фракций декстрана |
| EA017152B1 (ru) * | 2006-08-28 | 2012-10-30 | Арес Трейдинг С.А. | СПОСОБ ОЧИСТКИ Fc-СОДЕРЖАЩИХ БЕЛКОВ |
| US20140072560A1 (en) * | 2012-09-11 | 2014-03-13 | Coherus Biosciences, Inc. | Correctly folded etanercept in high purity and excellent yield |
| WO2014078627A1 (en) * | 2012-11-19 | 2014-05-22 | Merck Sharp & Dohme Corp. | Liquid formulations for tnfr:fc fusion proteins |
| US20150299249A1 (en) * | 2013-10-18 | 2015-10-22 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
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| US7294481B1 (en) | 1999-01-05 | 2007-11-13 | Immunex Corporation | Method for producing recombinant proteins |
| MXPA04008215A (es) | 2002-02-27 | 2004-11-26 | Immunex Corp | Formulacion polipeptidica. |
| AU2004215653B2 (en) | 2003-02-28 | 2011-03-17 | Lonza Biologics Plc. | Antibody purification by protein A and ion exchange chromatography |
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| US20100256337A1 (en) | 2007-10-22 | 2010-10-07 | Merck Serono Sa | Method for purifying an fc-containing protein |
| WO2009111347A1 (en) * | 2008-02-29 | 2009-09-11 | Biogen Idec Ma Inc. | Purified immunoglobulin fusion proteins and methods of their purification |
| KR20110079693A (ko) * | 2008-10-29 | 2011-07-07 | 와이어쓰 엘엘씨 | 단일 도메인 항원 결합 분자의 정제 방법 |
| EP2526114B1 (de) * | 2010-01-22 | 2014-06-18 | Boehringer Ingelheim International GmbH | Chromatographisches verfahren zur aufreinigung von fc enthaltenden proteinen |
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| JP6223338B2 (ja) * | 2011-08-17 | 2017-11-01 | アレス トレーディング ソシエテ アノニム | 活性型ＴＮＦＲ−Ｆｃ融合タンパク質を製造する方法 |
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| CN102911250B (zh) * | 2012-09-29 | 2014-04-16 | 浙江海正药业股份有限公司 | 酸性重组蛋白药物的纯化方法 |
| ES2964601T3 (es) * | 2013-03-14 | 2024-04-08 | Amgen Inc | Eliminación de ligando de purificación por afinidad filtrado |
-
2015
- 2015-06-13 EP EP15734731.1A patent/EP3155009A1/en not_active Withdrawn
- 2015-06-13 CN CN201580038801.XA patent/CN106536565A/zh active Pending
- 2015-06-13 BR BR112016029157A patent/BR112016029157A8/pt not_active Application Discontinuation
- 2015-06-13 CA CA2951766A patent/CA2951766A1/en not_active Abandoned
- 2015-06-13 WO PCT/IB2015/054494 patent/WO2015189832A1/en not_active Ceased
- 2015-06-13 MX MX2016016318A patent/MX2016016318A/es unknown
- 2015-06-13 JP JP2016572703A patent/JP6747985B2/ja not_active Expired - Fee Related
- 2015-06-13 US US15/318,489 patent/US10556942B2/en active Active
- 2015-06-13 AU AU2015273049A patent/AU2015273049B2/en not_active Ceased
- 2015-06-13 RU RU2017100005A patent/RU2698654C2/ru active
- 2015-06-13 MA MA040232A patent/MA40232A/fr unknown
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2016
- 2016-12-13 PH PH12016502482A patent/PH12016502482A1/en unknown
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| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2021284C1 (ru) * | 1991-05-30 | 1994-10-15 | Всесоюзный научно-исследовательский институт технологии кровезаменителей и гормональных препаратов | Способ очистки клинических фракций декстрана |
| EA017152B1 (ru) * | 2006-08-28 | 2012-10-30 | Арес Трейдинг С.А. | СПОСОБ ОЧИСТКИ Fc-СОДЕРЖАЩИХ БЕЛКОВ |
| US20140072560A1 (en) * | 2012-09-11 | 2014-03-13 | Coherus Biosciences, Inc. | Correctly folded etanercept in high purity and excellent yield |
| WO2014078627A1 (en) * | 2012-11-19 | 2014-05-22 | Merck Sharp & Dohme Corp. | Liquid formulations for tnfr:fc fusion proteins |
| US20150299249A1 (en) * | 2013-10-18 | 2015-10-22 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2849383C2 (ru) * | 2021-10-19 | 2025-10-24 | Альтеоджен, Инк | Способ очистки слитого белка, имеющего Fc-домен IgG |
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| MX2016016318A (es) | 2017-06-12 |
| US20170152298A1 (en) | 2017-06-01 |
| EP3155009A1 (en) | 2017-04-19 |
| US10556942B2 (en) | 2020-02-11 |
| JP6747985B2 (ja) | 2020-08-26 |
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| AU2015273049A1 (en) | 2017-01-12 |
| JP2017521389A (ja) | 2017-08-03 |
| AU2015273049B2 (en) | 2019-11-14 |
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| WO2015189832A1 (en) | 2015-12-17 |
| CN106536565A (zh) | 2017-03-22 |
| BR112016029157A8 (pt) | 2021-07-06 |
| RU2017100005A (ru) | 2018-07-13 |
| CA2951766A1 (en) | 2015-12-17 |
| PH12016502482A1 (en) | 2017-04-10 |
| BR112016029157A2 (pt) | 2017-08-22 |
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