JP2014521364A5 - - Google Patents
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- Publication number
- JP2014521364A5 JP2014521364A5 JP2014525943A JP2014525943A JP2014521364A5 JP 2014521364 A5 JP2014521364 A5 JP 2014521364A5 JP 2014525943 A JP2014525943 A JP 2014525943A JP 2014525943 A JP2014525943 A JP 2014525943A JP 2014521364 A5 JP2014521364 A5 JP 2014521364A5
- Authority
- JP
- Japan
- Prior art keywords
- sodium
- tnfr
- buffer
- fusion protein
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 23
- 108010008165 Etanercept Proteins 0.000 claims 13
- 239000006167 equilibration buffer Substances 0.000 claims 9
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 7
- 239000012149 elution buffer Substances 0.000 claims 7
- 239000011734 sodium Substances 0.000 claims 7
- 229910052708 sodium Inorganic materials 0.000 claims 7
- 239000000203 mixture Substances 0.000 claims 6
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims 5
- 150000003839 salts Chemical class 0.000 claims 5
- 238000002347 injection Methods 0.000 claims 4
- 239000007924 injection Substances 0.000 claims 4
- 229910052938 sodium sulfate Inorganic materials 0.000 claims 4
- 235000011152 sodium sulphate Nutrition 0.000 claims 4
- HHLMIPMLRQMODA-UHFFFAOYSA-M [Na+].OP(O)(O)=O.OS([O-])(=O)=O Chemical compound [Na+].OP(O)(O)=O.OS([O-])(=O)=O HHLMIPMLRQMODA-UHFFFAOYSA-M 0.000 claims 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims 3
- 239000001509 sodium citrate Substances 0.000 claims 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 2
- 239000000872 buffer Substances 0.000 claims 2
- 239000007853 buffer solution Substances 0.000 claims 2
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 2
- 210000004962 mammalian cell Anatomy 0.000 claims 2
- 239000001488 sodium phosphate Substances 0.000 claims 2
- 229910000162 sodium phosphate Inorganic materials 0.000 claims 2
- FYKDNWHPKQOZOT-UHFFFAOYSA-M sodium;dihydrogen phosphate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].OP(O)([O-])=O.OC(=O)CC(O)(C(O)=O)CC(O)=O FYKDNWHPKQOZOT-UHFFFAOYSA-M 0.000 claims 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims 2
- 239000011534 wash buffer Substances 0.000 claims 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims 1
- 238000001042 affinity chromatography Methods 0.000 claims 1
- 125000000217 alkyl group Chemical group 0.000 claims 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims 1
- 235000011130 ammonium sulphate Nutrition 0.000 claims 1
- 238000011033 desalting Methods 0.000 claims 1
- 238000004255 ion exchange chromatography Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 125000002347 octyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims 1
- 239000011347 resin Substances 0.000 claims 1
- 229920005989 resin Polymers 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Images
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20110081854 | 2011-08-17 | ||
| KR10-2011-0081854 | 2011-08-17 | ||
| PCT/KR2012/006564 WO2013025079A1 (en) | 2011-08-17 | 2012-08-17 | Method for preparing active form of tnfr-fc fusion protein |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016105574A Division JP2016168049A (ja) | 2011-08-17 | 2016-05-26 | 活性型ＴＮＦＲ−Ｆｃ融合タンパク質を製造する方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014521364A JP2014521364A (ja) | 2014-08-28 |
| JP2014521364A5 true JP2014521364A5 (enExample) | 2015-10-29 |
| JP6223338B2 JP6223338B2 (ja) | 2017-11-01 |
Family
ID=47715279
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014525943A Expired - Fee Related JP6223338B2 (ja) | 2011-08-17 | 2012-08-17 | 活性型ＴＮＦＲ−Ｆｃ融合タンパク質を製造する方法 |
| JP2016105574A Pending JP2016168049A (ja) | 2011-08-17 | 2016-05-26 | 活性型ＴＮＦＲ−Ｆｃ融合タンパク質を製造する方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016105574A Pending JP2016168049A (ja) | 2011-08-17 | 2016-05-26 | 活性型ＴＮＦＲ−Ｆｃ融合タンパク質を製造する方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9279014B2 (enExample) |
| EP (1) | EP2753634B1 (enExample) |
| JP (2) | JP6223338B2 (enExample) |
| KR (1) | KR101454316B1 (enExample) |
| ES (1) | ES2651483T3 (enExample) |
| WO (1) | WO2013025079A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2012326170B2 (en) | 2011-10-18 | 2016-12-22 | Coherus Biosciences, Inc. | Etanercept formulations stabilized with metal ions |
| KR101494072B1 (ko) * | 2012-03-12 | 2015-02-17 | 한화케미칼 주식회사 | 변이된 글루타민 합성효소 유전자를 포함하는 벡터 및 이를 이용한 목적 단백질의 생산방법 |
| KR102250937B1 (ko) * | 2012-09-11 | 2021-05-12 | 코히러스 바이오사이언시즈, 인코포레이티드 | 고순도 및 탁월한 수율의 정확하게 폴딩된 에타너셉트 |
| KR20160002713A (ko) | 2013-02-13 | 2016-01-08 | 라보라토이레 프란카이즈 듀 프락티온네먼트 에트 데스 바이오테크놀로지스 | 고도로 갈락토실화된 항-tnf-알파 항체 및 이의 용도 |
| WO2015189832A1 (en) * | 2014-06-13 | 2015-12-17 | Lupin Limited | Process for the purification of tnfr:fc fusion protein |
| EP2975050B1 (en) | 2014-07-18 | 2019-05-29 | Sandoz Ag | Quantification of misfolded TNFR2:Fc |
| KR102018608B1 (ko) * | 2014-12-31 | 2019-09-06 | 주식회사 엘지화학 | 목적하는 함량으로 불순물을 포함하는 TNFR-Fc 융합 단백질의 제조방법 |
| US20180079796A1 (en) * | 2015-03-13 | 2018-03-22 | Samsung Bioepis Co., Ltd. | Anti-tnf-alpha polypeptide composition and use thereof |
| US11952399B2 (en) * | 2018-12-18 | 2024-04-09 | Amgen Inc. | Methods for purifying proteins |
| EP4147026A1 (en) | 2020-05-08 | 2023-03-15 | Waters Technologies Corporation | Methods for heat-assisted enzyme digestion |
| US12411064B2 (en) | 2020-05-08 | 2025-09-09 | Waters Technologies Corporation | Methods for heat-assisted enzyme digestion |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TR200504220T2 (tr) | 1998-12-17 | 2007-04-24 | Biogen Idec Ma Inc. | Aktif limfotoksin-beta reseptör imunoglobülin şimeAktif limfotoksin-beta reseptör imunoglobülin şimerik proteinlerinin yüksek düzey ifadesi ve saflaştrik proteinlerinin yüksek düzey ifadesi ve saflaştırılması için bir yöntem.ırılması için bir yöntem. |
| US7294481B1 (en) | 1999-01-05 | 2007-11-13 | Immunex Corporation | Method for producing recombinant proteins |
| CA2476934C (en) | 2002-02-27 | 2009-06-16 | Immunex Corporation | Stabilized tnfr-fc composition comprising arginine |
| US7427659B2 (en) * | 2003-10-24 | 2008-09-23 | Amgen Inc. | Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction |
| ES2377125T3 (es) | 2004-01-30 | 2012-03-22 | Amgen Inc. | Procedimiento de purificación de proteínas |
| JP2011514895A (ja) | 2008-02-29 | 2011-05-12 | バイオジェン・アイデック・エムエイ・インコーポレイテッド | 精製免疫グロブリン融合タンパク質およびその精製方法 |
| CN102675465A (zh) * | 2011-03-09 | 2012-09-19 | 上海赛金生物医药有限公司 | 重组人ⅱ型肿瘤坏死因子受体-抗体融合蛋白的纯化方法 |
| EP2723759A4 (en) * | 2011-06-24 | 2015-01-28 | Reddys Lab Ltd Dr | PURIFICATION OF CHIMERIC PROTEIN |
-
2012
- 2012-08-17 ES ES12823797.1T patent/ES2651483T3/es active Active
- 2012-08-17 KR KR1020120090341A patent/KR101454316B1/ko not_active Expired - Fee Related
- 2012-08-17 WO PCT/KR2012/006564 patent/WO2013025079A1/en not_active Ceased
- 2012-08-17 EP EP12823797.1A patent/EP2753634B1/en active Active
- 2012-08-17 US US14/239,216 patent/US9279014B2/en active Active
- 2012-08-17 JP JP2014525943A patent/JP6223338B2/ja not_active Expired - Fee Related
-
2016
- 2016-05-26 JP JP2016105574A patent/JP2016168049A/ja active Pending
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