ATE547521T1 - Verfahren zur reinigung von nukleinsäure - Google Patents
Verfahren zur reinigung von nukleinsäureInfo
- Publication number
- ATE547521T1 ATE547521T1 AT07842757T AT07842757T ATE547521T1 AT E547521 T1 ATE547521 T1 AT E547521T1 AT 07842757 T AT07842757 T AT 07842757T AT 07842757 T AT07842757 T AT 07842757T AT E547521 T1 ATE547521 T1 AT E547521T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- guanidine
- purifying nucleic
- anion exchange
- exchange resins
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 5
- 102000039446 nucleic acids Human genes 0.000 title abstract 5
- 108020004707 nucleic acids Proteins 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 4
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 abstract 2
- 239000003957 anion exchange resin Substances 0.000 abstract 2
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 abstract 1
- 239000000654 additive Substances 0.000 abstract 1
- 230000000996 additive effect Effects 0.000 abstract 1
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 238000005342 ion exchange Methods 0.000 abstract 1
- 238000011084 recovery Methods 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/20—Anion exchangers for chromatographic processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US82691606P | 2006-09-26 | 2006-09-26 | |
| PCT/US2007/078867 WO2008039668A1 (en) | 2006-09-26 | 2007-09-19 | Nucleic acid purification method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE547521T1 true ATE547521T1 (de) | 2012-03-15 |
Family
ID=38640517
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT07842757T ATE547521T1 (de) | 2006-09-26 | 2007-09-19 | Verfahren zur reinigung von nukleinsäure |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7655793B2 (de) |
| EP (1) | EP2069495B1 (de) |
| AT (1) | ATE547521T1 (de) |
| CA (1) | CA2664211A1 (de) |
| GB (1) | GB2443505B (de) |
| WO (1) | WO2008039668A1 (de) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101489655A (zh) | 2006-07-14 | 2009-07-22 | 威斯康星旧生研究基金会 | 捕获病毒用吸附膜 |
| WO2009100172A1 (en) | 2008-02-07 | 2009-08-13 | Ge Healthcare Bio-Sciences Corp. | Isolation of dna, rna and protein from a single sample |
| EP2588609B1 (de) | 2010-06-29 | 2018-01-17 | Exscale Biospecimen Solutions AB | Verfahren und kit zur sequenziellen isolation einer nukleotidspezies aus einer probe |
| CN104946626A (zh) * | 2011-01-12 | 2015-09-30 | 积水医疗株式会社 | 离子交换色谱法用洗脱液以及核酸链的分析方法 |
| CN103348242B (zh) * | 2011-01-12 | 2016-08-17 | 积水医疗株式会社 | 单核苷酸多态性的检测方法 |
| CN103764827B (zh) * | 2011-07-04 | 2018-04-24 | 恰根有限公司 | 可用于分离和／或纯化核酸的试剂 |
| CN103657617B (zh) * | 2012-09-14 | 2016-06-15 | 丁少峰 | 一种层析柱及核酸提取方法 |
| US9506897B2 (en) | 2012-11-16 | 2016-11-29 | Agilent Technologies, Inc. | Methods and compositions for improved ion-exchange chromatography |
| GB201718803D0 (en) * | 2017-11-14 | 2017-12-27 | Phoenix Molecular Pte Ltd | Method for isolation of nucleic acids |
| US11666888B2 (en) | 2018-02-05 | 2023-06-06 | Bio-Rad Laboratories, Inc. | Chromatography resin having an anionic exchange-hydrophobic mixed mode ligand |
| CN108892699B (zh) * | 2018-07-23 | 2021-07-30 | 南通秋之友生物科技有限公司 | 一种高纯度核苷酸的精制方法 |
| CN109722467A (zh) * | 2019-01-21 | 2019-05-07 | 上海科华生物工程股份有限公司 | 碳酸氢钠缓冲体系在核酸提取中的应用 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4034036C2 (de) | 1990-10-26 | 1994-03-03 | Diagen Inst Molekularbio | Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen |
| US5866429A (en) | 1991-04-03 | 1999-02-02 | Bloch; Will | Precision and accuracy of anion-exchange separation of nucleic acids |
| DE4143639C2 (de) | 1991-12-02 | 2002-10-24 | Qiagen Gmbh | Verfahren zur Isolierung und Reinigung von Nukleinsäuren |
| US5561064A (en) | 1994-02-01 | 1996-10-01 | Vical Incorporated | Production of pharmaceutical-grade plasmid DNA |
| US5990301A (en) | 1994-02-07 | 1999-11-23 | Qiagen Gmbh | Process for the separation and purification of nucleic acids from biological sources |
| EP0749583B1 (de) | 1994-03-11 | 2002-08-28 | Abbott Laboratories | Cyanidfreies reagens und verfahren zur bestimmung von hämoglobin |
| US5567807A (en) | 1994-07-08 | 1996-10-22 | La Jolla Cancer Research Foundation | Processes for the purification of human recombinant decorin and the detection of guanidinium ions |
| US5660984A (en) * | 1994-12-09 | 1997-08-26 | Davis; Thomas E. | DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof |
| SE9500183D0 (sv) | 1995-01-20 | 1995-01-20 | Pharmacia Biotech Ab | Method for the purifivation of short nucleic acids |
| SE9600590D0 (sv) * | 1996-02-19 | 1996-02-19 | Pharmacia Biotech Ab | Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris |
| US5707812A (en) | 1996-08-06 | 1998-01-13 | Vical Incorporated | Purification of plasmid DNA during column chromatography |
| SE9703532D0 (sv) | 1997-09-30 | 1997-09-30 | Pharmacia Biotech Ab | A process for the purification of plasmid DNA |
| DE19746874A1 (de) * | 1997-10-23 | 1999-04-29 | Qiagen Gmbh | Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen |
| EP0992583B1 (de) | 1998-09-14 | 2003-03-26 | QIAGEN GmbH | Methode für die Reinigung kovalent geschlossener zirkulärer DNS |
| US6310199B1 (en) | 1999-05-14 | 2001-10-30 | Promega Corporation | pH dependent ion exchange matrix and method of use in the isolation of nucleic acids |
| AU1085601A (en) * | 1999-10-12 | 2001-04-23 | Transgenomic, Inc. | Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography |
| DE50008101D1 (de) | 2000-02-16 | 2004-11-11 | Macherey Nagel Gmbh & Co Hg | Verfahren zur Aufreinigung von Nukleinsäuren sowie Anionenaustauscher zur Durchführung dieses Verfahrens |
| EP1243649A1 (de) | 2001-03-23 | 2002-09-25 | GeneScan Europe AG | Verfahren zur Isolierung von DNA |
| CN1643145A (zh) | 2002-03-21 | 2005-07-20 | 艾夫西亚生物科技公司 | 寡核苷酸及其类似物的纯化方法 |
| US7964343B2 (en) | 2003-05-13 | 2011-06-21 | Ibis Biosciences, Inc. | Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
-
2007
- 2007-09-10 GB GB0717585A patent/GB2443505B/en not_active Expired - Fee Related
- 2007-09-19 AT AT07842757T patent/ATE547521T1/de active
- 2007-09-19 EP EP07842757A patent/EP2069495B1/de not_active Not-in-force
- 2007-09-19 US US11/857,727 patent/US7655793B2/en not_active Expired - Fee Related
- 2007-09-19 WO PCT/US2007/078867 patent/WO2008039668A1/en active Application Filing
- 2007-09-19 CA CA002664211A patent/CA2664211A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP2069495B1 (de) | 2012-02-29 |
| GB0717585D0 (en) | 2007-10-17 |
| US7655793B2 (en) | 2010-02-02 |
| GB2443505B (en) | 2008-12-31 |
| WO2008039668A1 (en) | 2008-04-03 |
| US20080076911A1 (en) | 2008-03-27 |
| GB2443505A (en) | 2008-05-07 |
| EP2069495A1 (de) | 2009-06-17 |
| CA2664211A1 (en) | 2008-04-03 |
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