MX2012007103A - Solucion y metodo de lavado por cromatografia de afinidad. - Google Patents

Solucion y metodo de lavado por cromatografia de afinidad.

Info

Publication number
MX2012007103A
MX2012007103A MX2012007103A MX2012007103A MX2012007103A MX 2012007103 A MX2012007103 A MX 2012007103A MX 2012007103 A MX2012007103 A MX 2012007103A MX 2012007103 A MX2012007103 A MX 2012007103A MX 2012007103 A MX2012007103 A MX 2012007103A
Authority
MX
Mexico
Prior art keywords
affinity chromatography
wash solution
arginine
eluate
maintaining
Prior art date
Application number
MX2012007103A
Other languages
English (en)
Inventor
Achim Frauenschuh
Kurt Bill
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43598315&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2012007103(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of MX2012007103A publication Critical patent/MX2012007103A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La invención proporciona un método de lavado por cromatografía de afinidad en el cual una solución de lavado que comprende arginina, o un derivado de arginina, y una sal no amortiguadora, preferentemente a pH alto, mayor que 8.0, es efectiva para eliminar las impurezas, tal como, especies de alto peso molecular y proteínas de células huésped, mientras que aumenta además la concentración del producto en el eluato y mantiene un alto porcentaje de rendimiento del producto recuperado.
MX2012007103A 2009-12-18 2010-12-17 Solucion y metodo de lavado por cromatografia de afinidad. MX2012007103A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28805909P 2009-12-18 2009-12-18
PCT/EP2010/070076 WO2011073389A1 (en) 2009-12-18 2010-12-17 Wash solution and method for affinity chromatography

Publications (1)

Publication Number Publication Date
MX2012007103A true MX2012007103A (es) 2012-07-17

Family

ID=43598315

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012007103A MX2012007103A (es) 2009-12-18 2010-12-17 Solucion y metodo de lavado por cromatografia de afinidad.

Country Status (18)

Country Link
US (3) US9505803B2 (es)
EP (2) EP3272764B1 (es)
JP (2) JP5909450B2 (es)
KR (1) KR101858266B1 (es)
CN (1) CN102725304B (es)
AU (1) AU2010332778B2 (es)
BR (1) BR112012014937B1 (es)
CA (1) CA2784278C (es)
DK (2) DK3272764T3 (es)
ES (1) ES2651065T3 (es)
IL (1) IL220164B (es)
LT (2) LT2513134T (es)
MX (1) MX2012007103A (es)
PL (1) PL2513134T3 (es)
PT (2) PT3272764T (es)
RU (1) RU2564569C2 (es)
SG (2) SG181771A1 (es)
WO (1) WO2011073389A1 (es)

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US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
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US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
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US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
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CN111344410B (zh) 2017-08-17 2023-09-15 济世易为生物有限公司 从宿主细胞半乳糖凝集素和其他污染物中纯化糖基化蛋白的方法
CN107793469B (zh) * 2017-10-16 2021-07-30 上海药明生物技术有限公司 一种去除宿主细胞蛋白含量的亲和纯化工艺
IL275418B1 (en) * 2017-12-21 2024-03-01 Genzyme Corp Methods for improved removal of impurities during protein A chromatography
CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
US11407838B2 (en) 2018-04-02 2022-08-09 Amgen Inc. Erenumab compositions and uses thereof
US11612885B2 (en) * 2018-05-08 2023-03-28 Waters Technologies Corporation Methods, compositions and kits useful for pH gradient cation exchange chromatography
CA3150215A1 (en) * 2019-10-08 2021-04-15 Stefano Menegatti Immunoglobulin purification peptides and their use
WO2021191684A1 (en) * 2020-03-27 2021-09-30 Haemalogix Pty Ltd Composition and method
US20230143163A1 (en) * 2020-04-27 2023-05-11 Alexion Pharmaceuticals, Inc. Protein a chromatography purification of an antibody
CN112827217A (zh) * 2020-12-29 2021-05-25 苏州良辰生物医药科技有限公司 一种层析柱清洗液及其应用

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Also Published As

Publication number Publication date
KR20120109506A (ko) 2012-10-08
US20170044211A1 (en) 2017-02-16
EP3272764B1 (en) 2024-02-07
JP2016128495A (ja) 2016-07-14
RU2012130151A (ru) 2014-01-27
CN102725304A (zh) 2012-10-10
ES2651065T3 (es) 2018-01-24
PL2513134T3 (pl) 2018-02-28
LT3272764T (lt) 2024-05-10
CA2784278C (en) 2019-02-26
JP5909450B2 (ja) 2016-04-26
AU2010332778B2 (en) 2015-03-19
LT2513134T (lt) 2017-11-27
SG181771A1 (en) 2012-07-30
US9505803B2 (en) 2016-11-29
AU2010332778A1 (en) 2012-07-19
EP2513134B1 (en) 2017-09-06
BR112012014937A2 (pt) 2016-03-08
US20190233469A1 (en) 2019-08-01
PT2513134T (pt) 2017-12-14
RU2564569C2 (ru) 2015-10-10
EP2513134A1 (en) 2012-10-24
PT3272764T (pt) 2024-04-24
KR101858266B1 (ko) 2018-05-15
IL220164B (en) 2019-02-28
WO2011073389A1 (en) 2011-06-23
DK2513134T3 (en) 2017-12-18
IL220164A0 (en) 2012-07-31
US20120283416A1 (en) 2012-11-08
BR112012014937B1 (pt) 2021-01-19
JP2013514073A (ja) 2013-04-25
SG10201408384PA (en) 2015-01-29
DK3272764T3 (da) 2024-05-06
CN102725304B (zh) 2015-12-09
EP3272764A1 (en) 2018-01-24
CA2784278A1 (en) 2011-06-23

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