BR112012014937A2 - solução de lavagem e método para cromatografia de afinidade - Google Patents

solução de lavagem e método para cromatografia de afinidade

Info

Publication number
BR112012014937A2
BR112012014937A2 BR112012014937A BR112012014937A BR112012014937A2 BR 112012014937 A2 BR112012014937 A2 BR 112012014937A2 BR 112012014937 A BR112012014937 A BR 112012014937A BR 112012014937 A BR112012014937 A BR 112012014937A BR 112012014937 A2 BR112012014937 A2 BR 112012014937A2
Authority
BR
Brazil
Prior art keywords
affinity chromatography
wash solution
wash
arginine
product
Prior art date
Application number
BR112012014937A
Other languages
English (en)
Other versions
BR112012014937B1 (pt
Inventor
Achim Frauenschuh
Kurt Bill
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43598315&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112012014937(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of BR112012014937A2 publication Critical patent/BR112012014937A2/pt
Publication of BR112012014937B1 publication Critical patent/BR112012014937B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses

Abstract

solução de lavagem e método para cromatografia de afinidade. a presente invenção refere-se a um método de lavagem para cromatografia de afinidade no qual uma solução de lavagem compreendendo arginina, ou um derivado de arginina, e um sal não tamponado, de preferência em ph elevado, superior a 8,0, é eficaz na remoção de impurezas, tal como espécies de altos peso molecular e proteínas de células hospedeiras, enquanto também aumenta a concentração de produto no eluato e mantém um rendimento percentual elevado de produto recuperado.
BR112012014937-7A 2009-12-18 2010-12-17 método de produção de um anticorpo, fragmento de anticorpo ou proteína de fusão fc purificada usando uma matriz de cromatografia de afinidade BR112012014937B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28805909P 2009-12-18 2009-12-18
US61/288,059 2009-12-18
PCT/EP2010/070076 WO2011073389A1 (en) 2009-12-18 2010-12-17 Wash solution and method for affinity chromatography

Publications (2)

Publication Number Publication Date
BR112012014937A2 true BR112012014937A2 (pt) 2016-03-08
BR112012014937B1 BR112012014937B1 (pt) 2021-01-19

Family

ID=43598315

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012014937-7A BR112012014937B1 (pt) 2009-12-18 2010-12-17 método de produção de um anticorpo, fragmento de anticorpo ou proteína de fusão fc purificada usando uma matriz de cromatografia de afinidade

Country Status (18)

Country Link
US (3) US9505803B2 (pt)
EP (2) EP3272764B1 (pt)
JP (2) JP5909450B2 (pt)
KR (1) KR101858266B1 (pt)
CN (1) CN102725304B (pt)
AU (1) AU2010332778B2 (pt)
BR (1) BR112012014937B1 (pt)
CA (1) CA2784278C (pt)
DK (2) DK2513134T3 (pt)
ES (1) ES2651065T3 (pt)
IL (1) IL220164B (pt)
LT (1) LT2513134T (pt)
MX (1) MX2012007103A (pt)
PL (1) PL2513134T3 (pt)
PT (2) PT3272764T (pt)
RU (1) RU2564569C2 (pt)
SG (2) SG181771A1 (pt)
WO (1) WO2011073389A1 (pt)

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EP3769083A1 (en) 2018-03-21 2021-01-27 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
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Also Published As

Publication number Publication date
CN102725304A (zh) 2012-10-10
AU2010332778A1 (en) 2012-07-19
IL220164A0 (en) 2012-07-31
EP2513134B1 (en) 2017-09-06
DK3272764T3 (da) 2024-05-06
AU2010332778B2 (en) 2015-03-19
WO2011073389A1 (en) 2011-06-23
ES2651065T3 (es) 2018-01-24
PL2513134T3 (pl) 2018-02-28
US9505803B2 (en) 2016-11-29
MX2012007103A (es) 2012-07-17
CN102725304B (zh) 2015-12-09
PT3272764T (pt) 2024-04-24
CA2784278A1 (en) 2011-06-23
EP3272764A1 (en) 2018-01-24
US20190233469A1 (en) 2019-08-01
LT2513134T (lt) 2017-11-27
PT2513134T (pt) 2017-12-14
BR112012014937B1 (pt) 2021-01-19
JP2016128495A (ja) 2016-07-14
SG10201408384PA (en) 2015-01-29
US20120283416A1 (en) 2012-11-08
EP3272764B1 (en) 2024-02-07
IL220164B (en) 2019-02-28
US20170044211A1 (en) 2017-02-16
JP2013514073A (ja) 2013-04-25
EP2513134A1 (en) 2012-10-24
DK2513134T3 (en) 2017-12-18
RU2564569C2 (ru) 2015-10-10
SG181771A1 (en) 2012-07-30
CA2784278C (en) 2019-02-26
RU2012130151A (ru) 2014-01-27
KR101858266B1 (ko) 2018-05-15
KR20120109506A (ko) 2012-10-08
JP5909450B2 (ja) 2016-04-26

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