BR112014015101A2 - cromatografia de membrana de troca iônica - Google Patents
cromatografia de membrana de troca iônicaInfo
- Publication number
- BR112014015101A2 BR112014015101A2 BR112014015101A BR112014015101A BR112014015101A2 BR 112014015101 A2 BR112014015101 A2 BR 112014015101A2 BR 112014015101 A BR112014015101 A BR 112014015101A BR 112014015101 A BR112014015101 A BR 112014015101A BR 112014015101 A2 BR112014015101 A2 BR 112014015101A2
- Authority
- BR
- Brazil
- Prior art keywords
- polypeptide
- membrane
- ion exchange
- effluent
- interest
- Prior art date
Links
- 239000003014 ion exchange membrane Substances 0.000 title abstract 9
- 238000011140 membrane chromatography Methods 0.000 title abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 18
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 18
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 18
- 239000012528 membrane Substances 0.000 abstract 10
- 239000000356 contaminant Substances 0.000 abstract 6
- 238000000746 purification Methods 0.000 abstract 5
- 230000002708 enhancing effect Effects 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 238000011210 chromatographic step Methods 0.000 abstract 2
- 238000004255 ion exchange chromatography Methods 0.000 abstract 2
- 150000002500 ions Chemical class 0.000 abstract 2
- 238000001742 protein purification Methods 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
Abstract
methods for enhancing downstream protein purification steps using ion exchange membrane chromatography abstract of the disclosure methods of enhancing efficiency of downstream chromatography steps for purification of proteins comprising: (a) passing a composition comprising a polypeptide of interest and various contaminants through an ion exchange membrane, wherein the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a ph sufficiently distinct from the pi of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and at least one contaminant, (b) overloading the ion exchange membrane such that at least one contaminant remains bound to the membrane while the polypeptide of interest is primarily in the effluent; (c) collecting the effluent from the ion exchange membrane comprising the polypeptide of interest; (d) subjecting the membrane effluent comprising the polypeptide of interest to a purification step of similar charge as the previous membrane, and (e) recovering the purified polypeptide from the effluent of the charged ion exchange chromatography purification step. tradução do resumo resumo cromatografia de membrana de troca iônica métodos para intensificar eficiência de etapas de cromatografia a jusante para purificação de proteínas compreendendo: (a) passar uma composição compreendendo um polipeptídeo de interesse e vários contaminantes através de uma membrana de troca iônica, em que o polipeptídeo e a membrana têm cargas opostas, em condições de operação compreendidas de um tampão tendo um ph suficientemente distinto do pi do polipeptídeo para intensificar a carga do polipeptídeo e uma intensidade iônica baixa eficaz para evitar a blindagem das cargas por íons de tampão, o que faz a membrana ligar o polipeptídeo e pelo menos um contaminante; (b) sobrecarregar a membrana de troca iônica, de modo que pelo menos um contaminante permaneça ligado à membrana enquanto o polipeptídeo de interesse está primariamente no efluente; (c) coletar o efluente da membrana de troca iônica compreendendo o polipeptídeo de interesse; (d) submeter o efluente de membrana compreendendo o polipeptídeo de interesse a uma etapa de purificação de carga similar à membrana anterior, e (e) recuperar o polipeptídeo purificado do efluente da etapa de purificação de cromatografia de troca iônica carregada.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161579285P | 2011-12-22 | 2011-12-22 | |
| PCT/US2012/070373 WO2013096322A1 (en) | 2011-12-22 | 2012-12-18 | Ion exchange membrane chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112014015101A2 true BR112014015101A2 (pt) | 2017-06-13 |
| BR112014015101A8 BR112014015101A8 (pt) | 2021-06-08 |
Family
ID=47430154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112014015101A BR112014015101A8 (pt) | 2011-12-22 | 2012-12-18 | métodos para intensificar eficiência de etapas de cromatografia a jusante para purificação de anticorpos |
Country Status (22)
| Country | Link |
|---|---|
| US (2) | US10364268B2 (pt) |
| EP (2) | EP3492486A1 (pt) |
| JP (5) | JP6605202B2 (pt) |
| KR (3) | KR20200008022A (pt) |
| CN (2) | CN104125963B (pt) |
| AR (1) | AR089362A1 (pt) |
| AU (3) | AU2012355356B2 (pt) |
| BR (1) | BR112014015101A8 (pt) |
| CA (1) | CA2859376C (pt) |
| ES (1) | ES2697676T3 (pt) |
| HK (1) | HK1203526A1 (pt) |
| HR (1) | HRP20181846T1 (pt) |
| IL (2) | IL267585B (pt) |
| MX (2) | MX360453B (pt) |
| MY (1) | MY172426A (pt) |
| PL (1) | PL2794635T3 (pt) |
| RU (1) | RU2648999C2 (pt) |
| SG (1) | SG11201403437TA (pt) |
| SI (1) | SI2794635T1 (pt) |
| TR (1) | TR201815709T4 (pt) |
| WO (1) | WO2013096322A1 (pt) |
| ZA (2) | ZA201404593B (pt) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TR201815709T4 (tr) * | 2011-12-22 | 2018-11-21 | Hoffmann La Roche | İyon değişim membranı kromatografisi. |
| CN110054661A (zh) | 2013-12-12 | 2019-07-26 | Emd密理博公司 | 使用含丙烯酰胺的过滤器分离蛋白 |
| TWI787590B (zh) * | 2013-12-27 | 2022-12-21 | 日商中外製藥股份有限公司 | 等電點低之抗體的精製方法 |
| CN104693303B (zh) * | 2015-02-28 | 2018-03-13 | 苏州金盟生物技术有限公司 | 一种蛋白制品中的核酸去除方法 |
| SG10202101105XA (en) | 2015-08-13 | 2021-03-30 | Amgen Inc | Charged depth filtration of antigen-binding proteins |
| GB201711481D0 (en) | 2017-07-17 | 2017-08-30 | Ucb Biopharma Sprl | Protein purification |
| EP3546475A1 (en) * | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
| US10792618B2 (en) | 2018-06-19 | 2020-10-06 | Sartorius Stedim Biotech Gmbh | Particle separation and/or purification of a fluid |
| GB201815405D0 (en) * | 2018-09-21 | 2018-11-07 | Fujifilm Mfg Europe Bv | Membranes suitable for detecting, filtering and/or purifying biomolecules |
| WO2020128797A1 (en) * | 2018-12-21 | 2020-06-25 | 3M Innovative Properties Company | Method for testing a chromatography device used for ion exchange |
| AR119264A1 (es) * | 2019-06-05 | 2021-12-09 | Genentech Inc | Método para reutilización de cromatografía |
| US11416468B2 (en) * | 2020-07-21 | 2022-08-16 | International Business Machines Corporation | Active-active system index management |
| CN116371011B (zh) * | 2023-05-26 | 2023-08-18 | 湖南杰萃生物技术有限公司 | 一种从桑叶中提取黄酮和生物碱的方法 |
Family Cites Families (79)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE266710C (pt) | ||||
| US3773919A (en) | 1969-10-23 | 1973-11-20 | Du Pont | Polylactide-drug mixtures |
| USRE30985E (en) | 1978-01-01 | 1982-06-29 | Serum-free cell culture media | |
| US4515893A (en) | 1979-04-26 | 1985-05-07 | Ortho Pharmaceutical Corporation | Hybrid cell line for producing complement-fixing monoclonal antibody to human T cells |
| US4560655A (en) | 1982-12-16 | 1985-12-24 | Immunex Corporation | Serum-free cell culture medium and process for making same |
| US4657866A (en) | 1982-12-21 | 1987-04-14 | Sudhir Kumar | Serum-free, synthetic, completely chemically defined tissue culture media |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| DD266710A3 (de) | 1983-06-06 | 1989-04-12 | Ve Forschungszentrum Biotechnologie | Verfahren zur biotechnischen Herstellung van alkalischer Phosphatase |
| US4767704A (en) | 1983-10-07 | 1988-08-30 | Columbia University In The City Of New York | Protein-free culture medium |
| JPS6150925A (ja) * | 1984-08-17 | 1986-03-13 | Toray Ind Inc | 抗原または抗体の精製方法 |
| US4879231A (en) | 1984-10-30 | 1989-11-07 | Phillips Petroleum Company | Transformation of yeasts of the genus pichia |
| GB8516415D0 (en) | 1985-06-28 | 1985-07-31 | Celltech Ltd | Culture of animal cells |
| US5091178A (en) | 1986-02-21 | 1992-02-25 | Oncogen | Tumor therapy with biologically active anti-tumor antibodies |
| US4927762A (en) | 1986-04-01 | 1990-05-22 | Cell Enterprises, Inc. | Cell culture medium with antioxidant |
| GB8610600D0 (en) | 1986-04-30 | 1986-06-04 | Novo Industri As | Transformation of trichoderma |
| IL85035A0 (en) | 1987-01-08 | 1988-06-30 | Int Genetic Eng | Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same |
| AU626008B2 (en) * | 1987-10-23 | 1992-07-23 | Schering Corporation | Method of purifying protein |
| US5091313A (en) | 1988-08-05 | 1992-02-25 | Tanox Biosystems, Inc. | Antigenic epitopes of IgE present on B cell but not basophil surface |
| US5720937A (en) | 1988-01-12 | 1998-02-24 | Genentech, Inc. | In vivo tumor detection assay |
| DE68919361T2 (de) | 1988-06-21 | 1995-05-24 | Genentech Inc | Therapeutische zusammensetzungen für die behandlung von myocard-infarkten. |
| DE68925971T2 (de) | 1988-09-23 | 1996-09-05 | Cetus Oncology Corp | Zellenzuchtmedium für erhöhtes zellenwachstum, zur erhöhung der langlebigkeit und expression der produkte |
| US5530101A (en) | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
| JP2844346B2 (ja) * | 1989-05-31 | 1999-01-06 | 昭 梶 | 新規ペプチドならびに新規ｄｎａ |
| EP0402226A1 (en) | 1989-06-06 | 1990-12-12 | Institut National De La Recherche Agronomique | Transformation vectors for yeast yarrowia |
| DE3920358A1 (de) | 1989-06-22 | 1991-01-17 | Behringwerke Ag | Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung |
| US5122469A (en) | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| CA2113813C (en) | 1991-08-14 | 2005-04-12 | Paula M. Jardieu | Immunoglobulin variants for specific fc epsilon receptors |
| JPH07501451A (ja) | 1991-11-25 | 1995-02-16 | エンゾン・インコーポレイテッド | 多価抗原結合タンパク質 |
| AU675929B2 (en) | 1992-02-06 | 1997-02-27 | Curis, Inc. | Biosynthetic binding protein for cancer marker |
| MX9305070A (es) | 1992-08-21 | 1994-04-29 | Genentech Inc | Compocicion farmaceutica que contiene un antagonista de lfa-1 para el tratamiento de transtornos o desordenes mediados por el lfa-1 |
| US5736137A (en) | 1992-11-13 | 1998-04-07 | Idec Pharmaceuticals Corporation | Therapeutic application of chimeric and radiolabeled antibodies to human B lymphocyte restricted differentiation antigen for treatment of B cell lymphoma |
| US5595721A (en) | 1993-09-16 | 1997-01-21 | Coulter Pharmaceutical, Inc. | Radioimmunotherapy of lymphoma using anti-CD20 |
| HU218159B (hu) * | 1993-09-21 | 2000-06-28 | Hemosol Inc. | Kiszorításos kromatográfiás eljárás tisztított hemoglobintermék előállítására |
| WO1995016203A2 (en) | 1993-12-10 | 1995-06-15 | Genentech, Inc. | Methods for diagnosis of allergy and screening of anti-allergy therapeutics |
| DK0739214T3 (da) | 1994-01-18 | 1998-10-07 | Genentech Inc | Fremgangsmåde til behandling af parasitinfektionerunder anvendelse af IgE antagonister |
| WO1995023865A1 (en) | 1994-03-03 | 1995-09-08 | Genentech, Inc. | Anti-il-8 monoclonal antibodies for treatment of inflammatory disorders |
| US5534615A (en) | 1994-04-25 | 1996-07-09 | Genentech, Inc. | Cardiac hypertrophy factor and uses therefor |
| IL117645A (en) | 1995-03-30 | 2005-08-31 | Genentech Inc | Vascular endothelial cell growth factor antagonists for use as medicaments in the treatment of age-related macular degeneration |
| US5641870A (en) | 1995-04-20 | 1997-06-24 | Genentech, Inc. | Low pH hydrophobic interaction chromatography for antibody purification |
| CA2222231A1 (en) | 1995-06-07 | 1996-12-19 | Imclone Systems Incorporated | Antibody and antibody fragments for inhibiting the growth of tumors |
| EP1297847A3 (en) | 1996-01-23 | 2003-05-07 | Genentech, Inc. | Anti-CD18 antibodies in stroke |
| WO1997027757A1 (en) * | 1996-01-31 | 1997-08-07 | John Stephen Ayers | Production of an immunoglobulin enriched fraction from whey protein solutions |
| US7147851B1 (en) | 1996-08-15 | 2006-12-12 | Millennium Pharmaceuticals, Inc. | Humanized immunoglobulin reactive with α4β7 integrin |
| IL130143A0 (en) | 1996-11-27 | 2000-06-01 | Genentech Inc | Humanized anti-CD11a antibodies |
| EP0973804B1 (en) | 1997-04-07 | 2006-12-27 | Genentech, Inc. | Anti-vegf antibodies |
| PT1860187E (pt) | 1997-05-15 | 2011-10-04 | Genentech Inc | Receptor apo-2 |
| US6054293A (en) | 1997-07-08 | 2000-04-25 | The Regents Of The University Of California | Semaphorin receptors |
| US6946129B1 (en) | 1999-06-08 | 2005-09-20 | Seattle Genetics, Inc. | Recombinant anti-CD40 antibody and uses thereof |
| EP1189641B1 (en) | 1999-06-25 | 2009-07-29 | Genentech, Inc. | HUMANIZED ANTI-ErbB2 ANTIBODIES AND TREATMENT WITH ANTI-ErbB2 ANTIBODIES |
| IL149116A0 (en) | 1999-10-29 | 2002-11-10 | Genentech Inc | Anti-prostate stem cell antigen (psca) antibody compositions and methods of use |
| US20020012982A1 (en) | 2000-07-13 | 2002-01-31 | Invitrogen Corporation | Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix |
| UA83458C2 (uk) | 2000-09-18 | 2008-07-25 | Байоджен Айдек Ма Інк. | Виділений поліпептид baff-r (рецептор фактора активації в-клітин сімейства tnf) |
| US7321026B2 (en) | 2001-06-27 | 2008-01-22 | Skytech Technology Limited | Framework-patched immunoglobulins |
| WO2003025156A2 (en) | 2001-09-18 | 2003-03-27 | Affinium Pharmaceuticals, Inc. | Methods and apparatuses for purification |
| WO2003033656A2 (en) | 2001-10-16 | 2003-04-24 | Exelixis, Inc. | MSREBPs AS MODIFIERS OF THE SREBP PATHWAY AND METHODS OF USE |
| AU2002351495A1 (en) | 2001-10-17 | 2003-04-28 | Human Genome Sciences, Inc. | Neutrokine-alpha and neutrokine-alpha splice variant |
| US20040093621A1 (en) | 2001-12-25 | 2004-05-13 | Kyowa Hakko Kogyo Co., Ltd | Antibody composition which specifically binds to CD20 |
| CA2476166C (en) | 2002-02-14 | 2011-11-15 | Immunomedics, Inc. | Anti-cd20 antibodies and fusion proteins thereof and methods of use |
| SI1558648T1 (sl) | 2002-10-17 | 2012-05-31 | Genmab As | Človeška monoklonalna protitelesa proti CD |
| DE60332957D1 (de) | 2002-12-16 | 2010-07-22 | Genentech Inc | Immunoglobulinvarianten und deren verwendungen |
| CN1206240C (zh) * | 2003-04-07 | 2005-06-15 | 中国科学院生态环境研究中心 | 高效离子交换膜色谱纯化卵黄蛋白原技术 |
| WO2005000901A2 (en) | 2003-05-09 | 2005-01-06 | Duke University | Cd20-specific antibodies and methods of employing same |
| AR044388A1 (es) | 2003-05-20 | 2005-09-07 | Applied Molecular Evolution | Moleculas de union a cd20 |
| AU2004263538B2 (en) | 2003-08-08 | 2009-09-17 | Immunomedics, Inc. | Bispecific antibodies for inducing apoptosis of tumor and diseased cells |
| US8147832B2 (en) | 2003-08-14 | 2012-04-03 | Merck Patent Gmbh | CD20-binding polypeptide compositions and methods |
| US20080274501A1 (en) * | 2007-05-02 | 2008-11-06 | Chenming Zhang | Method of purifying acidic proteins expressed in plants |
| US9433922B2 (en) | 2007-08-14 | 2016-09-06 | Emd Millipore Corporation | Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same |
| CN101835791A (zh) | 2007-10-26 | 2010-09-15 | 旭化成化学株式会社 | 蛋白质的提纯方法 |
| RS53850B2 (sr) | 2007-10-30 | 2018-07-31 | Genentech Inc | Pročišćavanje antitela hromatografijom izmene katjona |
| WO2009135656A1 (en) | 2008-05-06 | 2009-11-12 | Lonza Biologics Plc. | A method for the purification of antibodies using displacement chromatography |
| HUE047316T2 (hu) | 2008-08-14 | 2020-04-28 | Genentech Inc | Eljárások szennyezés eltávolítására indigén fehérjekiszorításos ioncserés membrán kromatográfia alkalmazásával |
| GB0816242D0 (en) | 2008-09-05 | 2008-10-15 | Immunobiology Ltd | Method of purifying protien complexes |
| MX346115B (es) | 2009-08-06 | 2017-03-08 | Genentech Inc * | Metodo para mejorar la eliminación de virus en la purificacion proteica. |
| US9428548B2 (en) * | 2009-09-01 | 2016-08-30 | Genentech, Inc. | Enhanced protein purification through a modified protein A elution |
| ES2617777T5 (es) * | 2010-04-23 | 2022-10-13 | Hoffmann La Roche | Producción de proteínas heteromultiméricas |
| ES2637613T5 (es) * | 2010-05-25 | 2022-07-20 | Hoffmann La Roche | Procedimientos de purificación de polipéptidos |
| CA2850728A1 (en) * | 2011-10-03 | 2013-04-11 | Sachem, Inc. | Neutral zwitterionic displacer molecules for hydrophobic displacement chromatography |
| TR201815709T4 (tr) | 2011-12-22 | 2018-11-21 | Hoffmann La Roche | İyon değişim membranı kromatografisi. |
| JP5731726B1 (ja) | 2014-06-26 | 2015-06-10 | 楽天株式会社 | 情報処理装置、情報処理方法及び情報処理プログラム |
-
2012
- 2012-12-18 TR TR2018/15709T patent/TR201815709T4/tr unknown
- 2012-12-18 CN CN201280070369.9A patent/CN104125963B/zh active Active
- 2012-12-18 MX MX2014007622A patent/MX360453B/es active IP Right Grant
- 2012-12-18 PL PL12806322T patent/PL2794635T3/pl unknown
- 2012-12-18 KR KR1020207000846A patent/KR20200008022A/ko not_active IP Right Cessation
- 2012-12-18 AU AU2012355356A patent/AU2012355356B2/en active Active
- 2012-12-18 IL IL267585A patent/IL267585B/en unknown
- 2012-12-18 SI SI201231443T patent/SI2794635T1/sl unknown
- 2012-12-18 US US14/365,449 patent/US10364268B2/en active Active
- 2012-12-18 RU RU2014130017A patent/RU2648999C2/ru active
- 2012-12-18 KR KR1020147019968A patent/KR102067075B1/ko active IP Right Grant
- 2012-12-18 KR KR1020217005616A patent/KR20210025692A/ko not_active Application Discontinuation
- 2012-12-18 ES ES12806322T patent/ES2697676T3/es active Active
- 2012-12-18 JP JP2014549204A patent/JP6605202B2/ja active Active
- 2012-12-18 EP EP18193475.3A patent/EP3492486A1/en active Pending
- 2012-12-18 CN CN201810818291.3A patent/CN108892706A/zh active Pending
- 2012-12-18 BR BR112014015101A patent/BR112014015101A8/pt not_active Application Discontinuation
- 2012-12-18 WO PCT/US2012/070373 patent/WO2013096322A1/en active Application Filing
- 2012-12-18 MY MYPI2014701665A patent/MY172426A/en unknown
- 2012-12-18 SG SG11201403437TA patent/SG11201403437TA/en unknown
- 2012-12-18 CA CA2859376A patent/CA2859376C/en active Active
- 2012-12-18 EP EP12806322.9A patent/EP2794635B8/en active Active
- 2012-12-20 AR ARP120104862A patent/AR089362A1/es not_active Application Discontinuation
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2014
- 2014-06-16 IL IL233158A patent/IL233158B/en active IP Right Grant
- 2014-06-20 MX MX2018013356A patent/MX2018013356A/es unknown
- 2014-06-23 ZA ZA2014/04593A patent/ZA201404593B/en unknown
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2015
- 2015-04-27 HK HK15104046.9A patent/HK1203526A1/xx unknown
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2018
- 2018-01-10 AU AU2018200194A patent/AU2018200194B2/en active Active
- 2018-02-13 JP JP2018023128A patent/JP2018109024A/ja not_active Withdrawn
- 2018-11-05 HR HRP20181846TT patent/HRP20181846T1/hr unknown
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2019
- 2019-06-06 US US16/433,763 patent/US11945837B2/en active Active
- 2019-06-20 ZA ZA2019/03994A patent/ZA201903994B/en unknown
- 2019-10-24 JP JP2019193276A patent/JP7402011B2/ja active Active
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2020
- 2020-02-14 AU AU2020201070A patent/AU2020201070A1/en not_active Abandoned
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2021
- 2021-06-22 JP JP2021102974A patent/JP7359807B2/ja active Active
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2022
- 2022-03-25 JP JP2022050501A patent/JP2022101557A/ja active Pending
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