CY1122221T1 - Μεθοδοι αφαιρεσης ρυπου χρησιμοποιωντας ιοντοανταλλακτικη μεμβρανικη χρωματογραφια μετατοπισης γηγενων πρωτεϊνων - Google Patents

Μεθοδοι αφαιρεσης ρυπου χρησιμοποιωντας ιοντοανταλλακτικη μεμβρανικη χρωματογραφια μετατοπισης γηγενων πρωτεϊνων

Info

Publication number
CY1122221T1
CY1122221T1 CY20191101072T CY191101072T CY1122221T1 CY 1122221 T1 CY1122221 T1 CY 1122221T1 CY 20191101072 T CY20191101072 T CY 20191101072T CY 191101072 T CY191101072 T CY 191101072T CY 1122221 T1 CY1122221 T1 CY 1122221T1
Authority
CY
Cyprus
Prior art keywords
polypeptide
exchange membrane
ion
rough
proteins
Prior art date
Application number
CY20191101072T
Other languages
English (en)
Inventor
Arick Brown
Jerome Bill, Jr.
Timothy Tully
Christopher Dowd
Original Assignee
Genentech, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=39899038&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CY1122221(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genentech, Inc. filed Critical Genentech, Inc.
Publication of CY1122221T1 publication Critical patent/CY1122221T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation

Abstract

Περιγράφονται μέθοδοι καθαρισμού πολυπεπτιδίου από σύνθεση που περιλαμβάνει το πολυπεπτίδιο και τουλάχιστον ένα ρύπο, οι οποίες μέθοδοι περιλαμβάνουν τα εξής διαδοχικά στάδια: (a) διέλευση της σύνθεσης μέσω ιοντοανταλλακτικής μεμβράνης, όπου το πολυπεπτίδιο και η μεμβράνη έχουν αντίθετα φορτία, υπό συνθήκες λειτουργίας που αποτελούνται από ρυθμιστικό διάλυμα που έχει pΗ επαρκώς διαφορετικό από το ρI του πολυπεπτιδίου για να ενισχύεται το φορτίο του πολυπεπτιδίου και χαμηλή ιοντική ισχύ αποτελεσματική στο να αποτρέπει την θωράκιση φορτίων από ιόντα ρυθμιστικού διαλύματος, που προκαλούν την δέσμευση στην μεμβράνη του πολυπεπτιδίου και τουλάχιστον ενός ρύπου, και (b) ανάκτηση του καθαρισμένου πολυπεπτιδίου από την εκροή.
CY20191101072T 2008-08-14 2019-10-11 Μεθοδοι αφαιρεσης ρυπου χρησιμοποιωντας ιοντοανταλλακτικη μεμβρανικη χρωματογραφια μετατοπισης γηγενων πρωτεϊνων CY1122221T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08797895.3A EP2321337B2 (en) 2008-08-14 2008-08-14 Methods for removing a contaminant using protein displacement ion exchange membrane chromatography
EP14189582.1A EP2848625B1 (en) 2008-08-14 2008-08-14 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography
PCT/US2008/073179 WO2010019148A1 (en) 2008-08-14 2008-08-14 Methods for removing a contaminant using indigenous protein displacement ion exchange membrane chromatography

Publications (1)

Publication Number Publication Date
CY1122221T1 true CY1122221T1 (el) 2020-11-25

Family

ID=39899038

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20191101072T CY1122221T1 (el) 2008-08-14 2019-10-11 Μεθοδοι αφαιρεσης ρυπου χρησιμοποιωντας ιοντοανταλλακτικη μεμβρανικη χρωματογραφια μετατοπισης γηγενων πρωτεϊνων

Country Status (22)

Country Link
US (2) US10927144B2 (el)
EP (3) EP2848625B1 (el)
JP (1) JP5666447B2 (el)
KR (3) KR101843915B1 (el)
CN (1) CN102149724B (el)
AU (1) AU2008360625C1 (el)
BR (1) BRPI0823046B1 (el)
CA (1) CA2731943C (el)
CY (1) CY1122221T1 (el)
DK (2) DK2848625T3 (el)
ES (2) ES2749190T3 (el)
HK (1) HK1210181A1 (el)
HR (1) HRP20191848T1 (el)
HU (1) HUE047316T2 (el)
IL (2) IL210912B (el)
LT (1) LT2848625T (el)
MX (1) MX2011001506A (el)
PL (1) PL2848625T3 (el)
PT (1) PT2848625T (el)
RS (1) RS59232B1 (el)
SI (1) SI2848625T1 (el)
WO (1) WO2010019148A1 (el)

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Publication number Publication date
HRP20191848T1 (hr) 2019-12-27
AU2008360625B2 (en) 2016-05-19
MX2011001506A (es) 2011-03-15
DK2848625T3 (da) 2019-10-07
EP2321337B2 (en) 2018-10-10
LT2848625T (lt) 2019-10-10
CA2731943C (en) 2016-11-29
BRPI0823046A2 (pt) 2015-07-28
EP3604324A1 (en) 2020-02-05
CN102149724A (zh) 2011-08-10
EP2321337B1 (en) 2014-10-22
EP2848625B1 (en) 2019-07-31
US10927144B2 (en) 2021-02-23
AU2008360625C1 (en) 2017-01-19
KR102196883B1 (ko) 2020-12-30
RS59232B1 (sr) 2019-10-31
IL262657B (en) 2021-03-25
EP2321337A1 (en) 2011-05-18
JP2011530592A (ja) 2011-12-22
KR20180137591A (ko) 2018-12-27
EP3604324B1 (en) 2024-02-28
EP2848625A1 (en) 2015-03-18
US20210284685A1 (en) 2021-09-16
EP2321337B9 (en) 2016-11-30
CN102149724B (zh) 2014-04-09
WO2010019148A1 (en) 2010-02-18
AU2008360625A1 (en) 2010-02-18
KR20110067099A (ko) 2011-06-21
IL210912A0 (en) 2011-04-28
SI2848625T1 (sl) 2019-11-29
PL2848625T3 (pl) 2020-01-31
IL262657A (en) 2018-12-31
US20120122759A1 (en) 2012-05-17
DK3604324T3 (da) 2024-04-08
KR101843915B1 (ko) 2018-04-02
BRPI0823046B1 (pt) 2021-12-14
HK1210181A1 (en) 2016-04-15
ES2749190T3 (es) 2020-03-19
ES2527943T5 (es) 2019-03-07
ES2527943T3 (es) 2015-02-02
JP5666447B2 (ja) 2015-02-12
PT2848625T (pt) 2019-10-25
CA2731943A1 (en) 2010-02-18
IL210912B (en) 2019-03-31
KR20180033311A (ko) 2018-04-02
HUE047316T2 (hu) 2020-04-28

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