CO6612194A2 - Métodos de purificaciñon de adamts 13 y otras proteinas recombinantes y sus composiciones - Google Patents
Métodos de purificaciñon de adamts 13 y otras proteinas recombinantes y sus composicionesInfo
- Publication number
- CO6612194A2 CO6612194A2 CO12025851A CO12025851A CO6612194A2 CO 6612194 A2 CO6612194 A2 CO 6612194A2 CO 12025851 A CO12025851 A CO 12025851A CO 12025851 A CO12025851 A CO 12025851A CO 6612194 A2 CO6612194 A2 CO 6612194A2
- Authority
- CO
- Colombia
- Prior art keywords
- protein
- adamts
- methods
- hydroxyapatite
- sample
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 6
- 239000000203 mixture Substances 0.000 title abstract 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 102000043853 ADAMTS13 Human genes 0.000 abstract 4
- 108091005670 ADAMTS13 Proteins 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 229910052588 hydroxylapatite Inorganic materials 0.000 abstract 2
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 abstract 2
- 230000003612 virological effect Effects 0.000 abstract 2
- 102000005741 Metalloproteases Human genes 0.000 abstract 1
- 108010006035 Metalloproteases Proteins 0.000 abstract 1
- 102000007614 Thrombospondin 1 Human genes 0.000 abstract 1
- 108010046722 Thrombospondin 1 Proteins 0.000 abstract 1
- 238000005571 anion exchange chromatography Methods 0.000 abstract 1
- 238000005341 cation exchange Methods 0.000 abstract 1
- 238000005277 cation exchange chromatography Methods 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 239000000356 contaminant Substances 0.000 abstract 1
- 238000011026 diafiltration Methods 0.000 abstract 1
- 230000002209 hydrophobic effect Effects 0.000 abstract 1
- 230000000415 inactivating effect Effects 0.000 abstract 1
- 230000002779 inactivation Effects 0.000 abstract 1
- 230000003993 interaction Effects 0.000 abstract 1
- 239000006228 supernatant Substances 0.000 abstract 1
- 238000000108 ultra-filtration Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6489—Metalloendopeptidases (3.4.24)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/12—Purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
- C12N7/04—Inactivation or attenuation; Producing viral sub-units
- C12N7/06—Inactivation or attenuation by chemical treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Sustainable Development (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Water Supply & Treatment (AREA)
- Diabetes (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Virology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
En la presente descripción se proporcionan métodos para purificar proteína desintegrina tipo A y metalopeptidasa con trombospondina 1, motivo 13 recombinante (ADAMTS13) de una muestra. El método comprende el enriquecimiento de la proteína ADAMTS 13 mediante el contacto cromatográfico de la muestra con hidroxiapatita, bajo condiciones que permitan que la proteína ADAMTS 13 aparezca en el eluato o sobrenadante proveniente de la hidroxiapatita. Los métodos pueden comprender además la cromatografía en tándem con una resma de interacción hidrofóbicalintercambio catiónico de modo mixto a la que se une la proteína ADAMTS 13. Las etapas adicionales opcionales involucran la ultrafiltración/diafiltración, cromatografia de intercambio aniónico, cromatografía de intercambio catiónico, e inactivación viral. En la presente descripción se proporcionan, además, métodos para inactivar los contaminantes virales en muestras de proteína, donde la proteína se inmoviliza sobre un soporte. En la presente descripción se proporcionan, además, composiciones de ADAMTS 13 preparadas de acuerdo con dichos métodos.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23030809P | 2009-07-31 | 2009-07-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO6612194A2 true CO6612194A2 (es) | 2013-02-01 |
Family
ID=42588355
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CO12025851A CO6612194A2 (es) | 2009-07-31 | 2012-02-14 | Métodos de purificaciñon de adamts 13 y otras proteinas recombinantes y sus composiciones |
Country Status (23)
| Country | Link |
|---|---|
| US (3) | US8945895B2 (es) |
| EP (3) | EP4299735A3 (es) |
| JP (6) | JP5907869B2 (es) |
| KR (7) | KR101883610B1 (es) |
| CN (3) | CN107988192A (es) |
| AU (1) | AU2010277491B2 (es) |
| BR (1) | BR112012002140A2 (es) |
| CA (2) | CA2769362A1 (es) |
| CO (1) | CO6612194A2 (es) |
| DK (1) | DK2459715T3 (es) |
| EA (3) | EA201992487A1 (es) |
| ES (1) | ES2763207T3 (es) |
| HR (1) | HRP20192303T1 (es) |
| HU (1) | HUE046909T2 (es) |
| IN (1) | IN2012DN00907A (es) |
| LT (1) | LT2459715T (es) |
| MX (2) | MX2012001263A (es) |
| NZ (2) | NZ597756A (es) |
| PL (1) | PL2459715T3 (es) |
| PT (1) | PT2459715T (es) |
| SG (2) | SG178175A1 (es) |
| SI (1) | SI2459715T1 (es) |
| WO (1) | WO2011012726A2 (es) |
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| CN101384728B (zh) | 2006-02-16 | 2013-05-22 | 三菱化学美迪恩斯株式会社 | 意识障碍患者的病态检测方法和检测试剂盒 |
| US7468258B2 (en) | 2006-03-07 | 2008-12-23 | Wake Forest University Health Sciences | Self-quenching homofluorophore compositions for detecting enzyme activity |
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| US8669045B2 (en) | 2007-02-13 | 2014-03-11 | Ajinomoto Co., Inc. | Method for inactivating viruses with slightly acidic arginine |
| JP5254973B2 (ja) | 2007-06-22 | 2013-08-07 | 一般財団法人化学及血清療法研究所 | 新規ａｄａｍｔｓ−１３改変体 |
| FR2918375B1 (fr) * | 2007-07-05 | 2009-10-16 | Lab Francais Du Fractionnement | Utilisation d'un support de chromatographie pour reduire la quantite d'adamts13 dans une solution derivee du plasma |
| US8399620B2 (en) | 2007-07-11 | 2013-03-19 | Novo Nordisk A/S | Purification of factor VIII using a mixed-mode or multimodal resin |
| EP2197421A1 (en) * | 2007-08-31 | 2010-06-23 | Amgen, Inc | Solid-state protein formulation |
| US20090130714A1 (en) * | 2007-09-24 | 2009-05-21 | Reliance Life Sciences Pvt.Ltd. | Process for purifying recombinanat tissue plasminogen activator (TPA) |
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