MX2020003798A - Metodos para la purificacion de arilsulfatasa a. - Google Patents

Metodos para la purificacion de arilsulfatasa a.

Info

Publication number
MX2020003798A
MX2020003798A MX2020003798A MX2020003798A MX2020003798A MX 2020003798 A MX2020003798 A MX 2020003798A MX 2020003798 A MX2020003798 A MX 2020003798A MX 2020003798 A MX2020003798 A MX 2020003798A MX 2020003798 A MX2020003798 A MX 2020003798A
Authority
MX
Mexico
Prior art keywords
asa
arylsulfatase
present
methods
purification
Prior art date
Application number
MX2020003798A
Other languages
English (en)
Inventor
Dave Nichols
- Garcia Igor Quinones
Bee Lin Cheang
Mei Huei Jang
Original Assignee
Takeda Pharmaceuticals Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Pharmaceuticals Co filed Critical Takeda Pharmaceuticals Co
Publication of MX2020003798A publication Critical patent/MX2020003798A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/06—Sulfuric ester hydrolases (3.1.6)
    • C12Y301/06001—Arylsulfatase (3.1.6.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/06—Sulfuric ester hydrolases (3.1.6)
    • C12Y301/06008—Cerebroside-sulfatase (3.1.6.8)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Hospice & Palliative Care (AREA)
  • Psychiatry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención proporciona, entre otras cosas, métodos mejorados para purificar la proteína de arilsulfatasa (ASA) producida de forma recombinante para la terapia de reemplazo de enzimas; la presente invención se basa, en parte, en el sorprendente descubrimiento de que la proteína de ASA recombinante se puede purificar a partir de materiales biológicos no procesados, tal como, medio de cultivo celular que contiene ASA, usando un proceso que implica tan pocas como cuatro columnas de cromatografía y únicamente una etapa de ultrafiltración/diafilt ración posterior a la cromatografía.
MX2020003798A 2013-01-09 2015-07-08 Metodos para la purificacion de arilsulfatasa a. MX2020003798A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361750693P 2013-01-09 2013-01-09

Publications (1)

Publication Number Publication Date
MX2020003798A true MX2020003798A (es) 2020-08-03

Family

ID=50002885

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2015008875A MX2015008875A (es) 2013-01-09 2014-01-09 Metodos para la purificacion de arilsulfatasa a.
MX2020003798A MX2020003798A (es) 2013-01-09 2015-07-08 Metodos para la purificacion de arilsulfatasa a.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2015008875A MX2015008875A (es) 2013-01-09 2014-01-09 Metodos para la purificacion de arilsulfatasa a.

Country Status (12)

Country Link
US (2) US11407984B2 (es)
EP (1) EP2943568B1 (es)
JP (5) JP6427502B2 (es)
CN (2) CN104903442A (es)
AU (3) AU2014205441B2 (es)
BR (1) BR112015015948B1 (es)
CA (1) CA2896979C (es)
EA (1) EA030551B1 (es)
ES (1) ES2768261T3 (es)
HK (2) HK1214622A1 (es)
MX (2) MX2015008875A (es)
WO (1) WO2014110246A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10336992B2 (en) 2011-07-08 2019-07-02 Shire Human Genetic Therapies, Inc. Methods for purification of arylsulfatase A
CA2896979C (en) 2013-01-09 2023-05-02 Shire Human Genetic Therapies, Inc. Methods for purification of arylsulfatase a
EP3236772A4 (en) * 2014-12-22 2018-10-17 Alexion Pharmaceuticals, Inc. Methods of purifying recombinant proteins
BR112018003127A2 (pt) * 2015-08-20 2018-09-25 Genentech Inc purificação de fkpa e usos do mesmo para produzir polipeptídeos recombinantes
US10669327B2 (en) 2016-03-16 2020-06-02 Phoenix Tissue Repair, Inc. Methods of purifying collagen 7
JP6957738B2 (ja) * 2017-08-31 2021-11-02 グリーン・クロス・コーポレイションＧｒｅｅｎ Ｃｒｏｓｓ Ｃｏｒｐ． スルファターゼタンパク質を精製する方法
JP2021508322A (ja) * 2017-12-19 2021-03-04 シャイアー ヒューマン ジェネティック セラピーズ インコーポレイテッド 精製されたアリールスルファターゼａおよびその組成物
CN112703010A (zh) * 2018-02-02 2021-04-23 恩真万特诊疗公司 用于治疗法伯病的方法
JP7465215B2 (ja) * 2018-04-12 2024-04-10 アムジェン インコーポレイテッド 安定なタンパク質組成物を製造する方法
CN112082832B (zh) * 2019-06-12 2022-03-29 华南理工大学 一种保存城市污水中硫酸酯结合体和葡萄糖苷醛酸结合体的方法
CN110632052A (zh) * 2019-10-25 2019-12-31 山西师范大学 土壤芳基硫酸酯酶活性的荧光光谱检测法
CN112759643B (zh) * 2021-03-17 2023-05-30 华兰生物工程股份有限公司 一种血清白蛋白的脱脂方法
CN116693659A (zh) * 2023-07-11 2023-09-05 浙江大学 一种分离重组人血白蛋白的两步混合模式层析方法

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4314997A (en) 1980-10-06 1982-02-09 Edward Shanbrom Purification of plasma protein products
US4315919A (en) 1980-10-06 1982-02-16 Edward Shanbrom Depyrogenation process
US4764369A (en) 1983-07-14 1988-08-16 New York Blood Center Inc. Undenatured virus-free biologically active protein derivatives
CA2041280A1 (en) 1990-05-10 1991-11-11 Yosuke Sawada Arylsulfatase
NO960163D0 (no) 1996-01-15 1996-01-15 Thomas Berg Mutasjonsdeteksjon av bovin
JP2002501032A (ja) 1998-01-27 2002-01-15 ヘム−バイオテック エイ／エス 急性間欠性ポルフィリン症（ａｉｐ）及び他のポルフィリン症の治療方法
PL196770B1 (pl) 1998-06-09 2008-01-31 Statens Seruminstitut Sposób oczyszczania immunoglobuliny, produkt immunoglobuliny i jego zastosowanie
JP4685238B2 (ja) 1998-06-09 2011-05-18 ツエー・エス・エル・ベーリング・アクチエンゲゼルシヤフト 静脈投与用免疫グロブリン及び他の免疫グロブリン生成物の製造法
AUPQ026799A0 (en) 1999-05-10 1999-06-03 Csl Limited Method of increasing protein stability by removing immunoglobulin aggregates
AU774603B2 (en) 1999-07-27 2004-07-01 Zymenex A/S Production of rhPBGD and new therapeutic methods for treating patients with acute intermittent porphyria (AIP) and other porphyric diseases
US6812339B1 (en) 2000-09-08 2004-11-02 Applera Corporation Polymorphisms in known genes associated with human disease, methods of detection and uses thereof
EP1409695A2 (en) 2000-11-15 2004-04-21 Genzyme Corporation Expression system for recombinant proteins
AU2002317701A1 (en) 2001-06-07 2002-12-16 Hemebiotech A/S Production of recombinant human lysosomal alpha-mannosidase
AU2002317700A1 (en) * 2001-06-07 2002-12-16 Hemebiotech A/S Production of recombinant human arylsulfatase a
WO2003002731A1 (en) 2001-06-29 2003-01-09 Hemebiotech A/S A PROCESS FOR PURIFICATION OF RECOMBINANT PORPHOBILINOGEN DEAMINASE (rhPBGD)
US7232670B2 (en) 2001-09-28 2007-06-19 St. Jude Children's Research Hospital Targeting proteins to cells expressing mannose receptors via expression in insect cells
AU2003222568B2 (en) 2002-01-11 2009-05-07 Bioasis Technologies, Inc. Use of P97 as an enzyme delivery system for the delivery of therapeutic lysosomal enzymes
AU2003212912A1 (en) 2002-02-04 2003-09-02 Millipore Corporation Process for removing protein aggregates and virus from a protein solution
EP2532742B1 (en) * 2004-01-30 2018-08-08 Shire Pharmaceuticals Ireland Limited Recombinant arylsulfatase A for reducing galatosyl sulphatide levels in a subject
PL1740204T3 (pl) 2004-04-01 2018-08-31 Chiesi Farmaceutici S.P.A. Lecznicze zastosowanie alfa-mannozydazy
US20060051347A1 (en) 2004-09-09 2006-03-09 Winter Charles M Process for concentration of antibodies and therapeutic products thereof
SI2628746T1 (sl) 2006-04-04 2019-04-30 Chiesi Farmaceutici S.P.A. Proces za koncentriranje polipeptida
MX2011004558A (es) * 2008-10-29 2011-06-01 Wyeth Llc Procedimientos para la purificacion de moleculas de union a antigeno de un unico dominio.
US8945895B2 (en) * 2009-07-31 2015-02-03 Baxter International Inc. Methods of purifying recombinant ADAMTS13 and other proteins and compositions thereof
SI2585104T1 (sl) 2010-06-25 2019-11-29 Shire Human Genetic Therapies Postopki in sestavki za dostavo arilsulfataze A
PL3103469T3 (pl) 2010-06-25 2021-09-06 Shire Human Genetic Therapies, Inc. Dostarczanie środków terapeutycznych do OUN
GB201012603D0 (en) * 2010-07-27 2010-09-08 Ucb Pharma Sa Protein purification
CA2896979C (en) 2013-01-09 2023-05-02 Shire Human Genetic Therapies, Inc. Methods for purification of arylsulfatase a

Also Published As

Publication number Publication date
HK1216429A1 (zh) 2016-11-11
AU2020201079A1 (en) 2020-03-05
CN104903442A (zh) 2015-09-09
AU2014205441A1 (en) 2015-06-25
US11884945B2 (en) 2024-01-30
EA030551B1 (ru) 2018-08-31
HK1214622A1 (zh) 2016-07-29
JP2024001160A (ja) 2024-01-09
EP2943568B1 (en) 2019-11-20
JP7407161B2 (ja) 2023-12-28
CA2896979C (en) 2023-05-02
US20150353904A1 (en) 2015-12-10
EA201590826A1 (ru) 2015-11-30
EP2943568A1 (en) 2015-11-18
ES2768261T3 (es) 2020-06-22
CN112126634A (zh) 2020-12-25
JP2018171067A (ja) 2018-11-08
US11407984B2 (en) 2022-08-09
JP6427502B2 (ja) 2018-11-21
JP2020015750A (ja) 2020-01-30
WO2014110246A1 (en) 2014-07-17
AU2022221475A1 (en) 2022-09-22
BR112015015948B1 (pt) 2022-05-17
US20230235303A1 (en) 2023-07-27
CA2896979A1 (en) 2014-07-17
BR112015015948A2 (es) 2017-08-15
MX2015008875A (es) 2015-10-22
JP2022023225A (ja) 2022-02-07
JP2016504040A (ja) 2016-02-12
AU2014205441B2 (en) 2019-12-12
AU2020201079B2 (en) 2022-05-26

Similar Documents

Publication Publication Date Title
MX2020003798A (es) Metodos para la purificacion de arilsulfatasa a.
PH12020500027A1 (en) Purification of iduronate-2-sulfatase
MX2020006117A (es) Integracion dirigida de acidos nucleicos.
NZ743008A (en) Compositions and methods for internalizing enzymes
WO2019035880A8 (en) EXOSOMES OF PURIFIED MESENCHYMAL STEM CELLS AND USES THEREOF
BR112015019879A2 (pt) Composições de espécies de variantes moduladas de lisina e métodos para produção e uso das mesmas
MX344970B (es) Medio de cultivo celular para la expresion de la proteina adamts.
WO2016022377A3 (en) Methods for modulating the glycosylation profile of recombinant proteins using dissolved oxygen
WO2012031263A3 (en) Cell culture system for bioreactor scale-up of cells
EA201492185A1 (ru) Способ получения рекомбинантной идуронат-2-сульфатазы
WO2014004540A8 (en) Method for engineering proteases and protein kinases
EA201990593A1 (ru) Непрерывный способ для снижения гетерогенности терапевтического белка
PH12016500060A1 (en) Expanded bed absorption methods for isolation of basic milk proteins including lactoferrin
EA201000945A1 (ru) Конструирование системы секреции на основе sm-белков
MX2015010887A (es) Formulaciones y metodos para aumentar la produccion de proteinas recombinantes.
EA201790362A1 (ru) Солюбилизированный фермент и его применения
MX2013008103A (es) Purificacion de inhibidor de proteasa alfa1 derivado de cultivo celular.
MX2022002774A (es) Método de captura y purificación de productos biológicos.
MX2021016092A (es) Metodos de purificacion de proteinas.
WO2012028753A8 (es) Procedimiento para la producción y purificación de proteínas recombinantes en plantas
MX2015004373A (es) Moleculas de acidos nucleicos para una mayor produccion de proteinas.
TH160179A (th) การทำไอดิลโรเนต-2-ซัลฟาเตสให้บริสุทธิ์
IN2013MU01384A (es)
AR100577A1 (es) Medios y métodos para el tratamiento de cmv