EA201492185A1 - Способ получения рекомбинантной идуронат-2-сульфатазы - Google Patents

Способ получения рекомбинантной идуронат-2-сульфатазы

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Publication number
EA201492185A1
EA201492185A1 EA201492185A EA201492185A EA201492185A1 EA 201492185 A1 EA201492185 A1 EA 201492185A1 EA 201492185 A EA201492185 A EA 201492185A EA 201492185 A EA201492185 A EA 201492185A EA 201492185 A1 EA201492185 A1 EA 201492185A1
Authority
EA
Eurasian Patent Office
Prior art keywords
iduronat
sulfatase
recombinant
obtaining recombinant
protein
Prior art date
Application number
EA201492185A
Other languages
English (en)
Inventor
Чунь ЧЖАН
Ференс Болдог
Original Assignee
Шир Хьюман Дженетик Терапис, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Шир Хьюман Дженетик Терапис, Инк. filed Critical Шир Хьюман Дженетик Терапис, Инк.
Publication of EA201492185A1 publication Critical patent/EA201492185A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/99—Oxidoreductases acting on sulfur groups as donors (1.8) with other acceptors (1.8.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/06—Sulfuric ester hydrolases (3.1.6)
    • C12Y301/06013—Iduronate-2-sulfatase (3.1.6.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/40—Nucleotides, nucleosides, bases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Diabetes (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

В настоящем изобретении, среди прочего, предложены способы и составы для крупномасштабного получения рекомбинантного белка I2S при помощи суспензионного культивирования клеток млекопитающих в бессывороточной среде. В частности, в настоящем изобретении применяются клетки млекопитающих, коэкспрессирующие рекомбинантный белок I2S и формилглицинобразующий фермент (FGE).
EA201492185A 2012-06-29 2013-06-28 Способ получения рекомбинантной идуронат-2-сульфатазы EA201492185A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261666712P 2012-06-29 2012-06-29
PCT/US2013/048601 WO2014005036A1 (en) 2012-06-29 2013-06-28 Method of producing recombinant iduronate-2-sulfatase

Publications (1)

Publication Number Publication Date
EA201492185A1 true EA201492185A1 (ru) 2015-10-30

Family

ID=49778402

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201492185A EA201492185A1 (ru) 2012-06-29 2013-06-28 Способ получения рекомбинантной идуронат-2-сульфатазы

Country Status (21)

Country Link
US (1) US20140004097A1 (ru)
EP (1) EP2867367A4 (ru)
JP (1) JP2015523074A (ru)
KR (1) KR20150037908A (ru)
CN (1) CN104583414A (ru)
AU (1) AU2013282417A1 (ru)
BR (1) BR112014032544A2 (ru)
CA (1) CA2877492A1 (ru)
CL (1) CL2014003569A1 (ru)
CO (1) CO7240395A2 (ru)
CR (1) CR20140587A (ru)
DO (1) DOP2014000297A (ru)
EA (1) EA201492185A1 (ru)
HK (2) HK1209458A1 (ru)
IL (1) IL236324A0 (ru)
IN (1) IN2014DN11272A (ru)
MX (1) MX2015000188A (ru)
PE (1) PE20150603A1 (ru)
PH (1) PH12014502870A1 (ru)
SG (1) SG11201408755TA (ru)
WO (1) WO2014005036A1 (ru)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160145589A1 (en) 2011-06-24 2016-05-26 Green Cross Corporation Composition and formulation comprising recombinant human iduronate-2-sulfatase and preparation method thereof
US9150841B2 (en) 2012-06-29 2015-10-06 Shire Human Genetic Therapies, Inc. Cells for producing recombinant iduronate-2-sulfatase
KR101380740B1 (ko) 2012-06-29 2014-04-11 쉐어 휴먼 제네텍 세러피스, 인코포레이티드 이듀로네이트-2-설파타제의 정제
JP6433424B2 (ja) * 2012-07-31 2018-12-05 バイオアシス テクノロジーズ インコーポレイテッド 脱リン酸化されたリソソーム蓄積症タンパク質およびその使用方法
BR112015022416A2 (pt) 2013-03-13 2017-10-24 Bioasis Technologies Inc fragmentos de p97 e seus usos
ES2762672T3 (es) * 2014-02-19 2020-05-25 Bioasis Technologies Inc Proteínas de fusión de P97-IDS
CA2973343C (en) 2015-02-05 2023-08-08 R.P. Scherer Technologies, Llc Activated formylglycine-generating enzymes and methods of producing and using the same
RU2768936C2 (ru) * 2015-10-07 2022-03-25 Джоэль УЙСЕНГА Нормализация функций биологических путей для защиты от и устранения нарушений, возникающих вследствие старения человека
KR20230080504A (ko) * 2016-07-25 2023-06-07 리플리겐 코포레이션 교번 접선 유동식의 신속한 수확
JP2021500857A (ja) 2017-10-02 2021-01-14 デナリ セラピューティクス インコーポレイテッドＤｅｎａｌｉ Ｔｈｅｒａｐｅｕｔｉｃｓ Ｉｎｃ． 酵素補充療法用酵素を含む融合タンパク質

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WO2000065070A2 (en) * 1999-04-26 2000-11-02 Genentech, Inc. Cell culture process for glycoproteins
US6890736B1 (en) * 2002-09-20 2005-05-10 Immunex Corporation Methods for producing proteins in cultured cells
EP2325301B1 (en) * 2003-02-11 2015-09-02 Shire Human Genetic Therapies, Inc. Diagnosis and treatment of multiple sulfatase deficiency and others using a formylglycine generating enzyme (FGE)
US20050019914A1 (en) * 2003-07-24 2005-01-27 Aventis Pharma Deutschland Gmbh Perfusion process for producing erythropoietin
WO2005113765A2 (en) * 2004-05-06 2005-12-01 Biomarin Pharmaceutical Inc. Methods of activation of sulfatases and methods and compositions of using the same
PT1885753E (pt) * 2005-06-03 2011-10-06 Ares Trading Sa Produção de proteína recombinante de ligação a il-18
AU2006322028B2 (en) * 2005-12-08 2013-06-06 Amgen Inc. Improved host cells and culture methods
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EP2485761B1 (en) * 2009-10-09 2019-02-27 Armagen, Inc. Methods and compositions for increasing iduronate 2-sulfatase activity in the cns
US20130196410A1 (en) * 2010-03-05 2013-08-01 Alnylam Pharmaceuticals, Inc Compositions and methods for modifying the glycosylation pattern of a polypeptide
WO2011163649A2 (en) 2010-06-25 2011-12-29 Shire Human Genetic Therapies, Inc Methods and compositions for cns delivery of iduronate-2-sulfatase
KR101158673B1 (ko) * 2011-06-24 2012-07-03 주식회사 지씨바이오 재조합 인간 이듀로네이트-2-설파타아제를 포함하는 조성물, 제제 및 이의 제조방법
US9150841B2 (en) * 2012-06-29 2015-10-06 Shire Human Genetic Therapies, Inc. Cells for producing recombinant iduronate-2-sulfatase

Also Published As

Publication number Publication date
CA2877492A1 (en) 2014-01-03
IL236324A0 (en) 2015-02-26
CL2014003569A1 (es) 2015-04-17
CO7240395A2 (es) 2015-04-17
WO2014005036A1 (en) 2014-01-03
SG11201408755TA (en) 2015-02-27
CN104583414A (zh) 2015-04-29
PH12014502870A1 (en) 2015-02-23
US20140004097A1 (en) 2014-01-02
HK1209790A1 (en) 2016-04-08
JP2015523074A (ja) 2015-08-13
AU2013282417A1 (en) 2015-01-29
CR20140587A (es) 2015-04-06
PE20150603A1 (es) 2015-05-27
EP2867367A4 (en) 2016-02-24
MX2015000188A (es) 2015-04-08
EP2867367A1 (en) 2015-05-06
IN2014DN11272A (ru) 2015-10-09
DOP2014000297A (es) 2015-04-15
KR20150037908A (ko) 2015-04-08
BR112014032544A2 (pt) 2017-08-01
HK1209458A1 (en) 2016-04-01

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