EA201492183A1 - Клетки для получения рекомбинантной идуронат-2-сульфатазы - Google Patents

Клетки для получения рекомбинантной идуронат-2-сульфатазы

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Publication number
EA201492183A1
EA201492183A1 EA201492183A EA201492183A EA201492183A1 EA 201492183 A1 EA201492183 A1 EA 201492183A1 EA 201492183 A EA201492183 A EA 201492183A EA 201492183 A EA201492183 A EA 201492183A EA 201492183 A1 EA201492183 A1 EA 201492183A1
Authority
EA
Eurasian Patent Office
Prior art keywords
cells
iduronat
sulfatase
recombinant
obtaining recombinant
Prior art date
Application number
EA201492183A
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English (en)
Other versions
EA035511B1 (ru
Inventor
Ференс Болдог
Майкл Хартлейн
Original Assignee
Шир Хьюман Дженетик Терапис, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Шир Хьюман Дженетик Терапис, Инк. filed Critical Шир Хьюман Дженетик Терапис, Инк.
Publication of EA201492183A1 publication Critical patent/EA201492183A1/ru
Publication of EA035511B1 publication Critical patent/EA035511B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/99—Oxidoreductases acting on sulfur groups as donors (1.8) with other acceptors (1.8.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/06—Sulfuric ester hydrolases (3.1.6)
    • C12Y301/06013—Iduronate-2-sulfatase (3.1.6.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

В изобретении, среди прочего, предложены способы и композиции для получения рекомбинантного белка I2S с улучшенными эффективностью и активностью при помощи клеток, коэкспрессирующих белок I2S и FGE. В некоторых вариантах реализации изобретения клетки согласно настоящему изобретению сконструированы так, чтобы одновременно сверхэкспрессировать рекомбинантные белки I2S и FGE. Клетки согласно изобретению адаптируемы ко многим условиям клеточного культивирования. В некоторых вариантах реализации изобретения клетки согласно настоящему изобретению адаптируемы к суспензионному бессывороточному культивированию в больших масштабах.
EA201492183A 2012-06-29 2013-06-28 Клетка и способ для получения рекомбинантной идуронат-2-сульфатазы EA035511B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261666719P 2012-06-29 2012-06-29
PCT/US2013/048571 WO2014005019A2 (en) 2012-06-29 2013-06-28 Cells for producing recombinant iduronate-2-sulfatase

Publications (2)

Publication Number Publication Date
EA201492183A1 true EA201492183A1 (ru) 2015-06-30
EA035511B1 EA035511B1 (ru) 2020-06-26

Family

ID=49778522

Family Applications (2)

Application Number Title Priority Date Filing Date
EA201492183A EA035511B1 (ru) 2012-06-29 2013-06-28 Клетка и способ для получения рекомбинантной идуронат-2-сульфатазы
EA202090805A EA202090805A1 (ru) 2012-06-29 2013-06-28 Клетки для получения рекомбинантной идуронат-2-сульфатазы

Family Applications After (1)

Application Number Title Priority Date Filing Date
EA202090805A EA202090805A1 (ru) 2012-06-29 2013-06-28 Клетки для получения рекомбинантной идуронат-2-сульфатазы

Country Status (27)

Country Link
US (3) US9150841B2 (ru)
EP (1) EP2874649B1 (ru)
JP (3) JP6067849B2 (ru)
KR (1) KR101710487B1 (ru)
CN (2) CN107267460A (ru)
AU (3) AU2013282400B2 (ru)
BR (1) BR112014032560B1 (ru)
CA (1) CA2877521C (ru)
CL (1) CL2014003568A1 (ru)
CO (1) CO7240394A2 (ru)
CR (1) CR20140586A (ru)
DO (1) DOP2014000298A (ru)
EA (2) EA035511B1 (ru)
ES (1) ES2718346T3 (ru)
GT (1) GT201400302A (ru)
HK (2) HK1209331A1 (ru)
IL (3) IL236343A (ru)
MX (2) MX366155B (ru)
MY (2) MY189314A (ru)
NZ (2) NZ734670A (ru)
PE (1) PE20150957A1 (ru)
PH (2) PH12014502868A1 (ru)
SG (2) SG11201408702VA (ru)
TR (1) TR201904435T4 (ru)
UA (1) UA118955C2 (ru)
WO (1) WO2014005019A2 (ru)
ZA (1) ZA201409396B (ru)

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Also Published As

Publication number Publication date
JP6302499B2 (ja) 2018-03-28
HK1209651A1 (en) 2016-04-08
AU2013282400B2 (en) 2016-02-04
ZA201409396B (en) 2018-05-30
JP6755272B2 (ja) 2020-09-16
JP6067849B2 (ja) 2017-01-25
EP2874649A4 (en) 2016-05-04
WO2014005019A2 (en) 2014-01-03
MY189314A (en) 2022-02-04
IL274266A (en) 2020-06-30
EP2874649A2 (en) 2015-05-27
MY157086A (en) 2016-04-19
SG11201408702VA (en) 2015-02-27
MX336716B (es) 2016-01-28
CA2877521C (en) 2021-06-01
US20140004593A1 (en) 2014-01-02
EA202090805A1 (ru) 2020-11-30
MX366155B (es) 2019-06-28
US20180010108A1 (en) 2018-01-11
EP2874649B1 (en) 2019-01-02
KR20150030242A (ko) 2015-03-19
JP2016105737A (ja) 2016-06-16
NZ734670A (en) 2019-12-20
CN104540517A (zh) 2015-04-22
US9719074B2 (en) 2017-08-01
PH12016500011A1 (en) 2019-08-14
CR20140586A (es) 2015-04-06
PH12014502868B1 (en) 2015-02-23
IL236343A (en) 2017-10-31
PH12014502868A1 (en) 2015-02-23
TR201904435T4 (tr) 2019-05-21
EA035511B1 (ru) 2020-06-26
BR112014032560A2 (pt) 2018-03-06
UA118955C2 (uk) 2019-04-10
AU2016200460B2 (en) 2017-11-02
NZ703137A (en) 2017-08-25
PE20150957A1 (es) 2015-07-15
AU2013282400A1 (en) 2015-01-29
HK1209331A1 (en) 2016-04-01
WO2014005019A3 (en) 2014-02-27
JP2018088933A (ja) 2018-06-14
CO7240394A2 (es) 2015-04-17
CN107267460A (zh) 2017-10-20
KR101710487B1 (ko) 2017-02-27
IL274266B (en) 2021-04-29
ES2718346T3 (es) 2019-07-01
DOP2014000298A (es) 2015-03-31
IL254560A0 (en) 2017-11-30
US20160053238A1 (en) 2016-02-25
CL2014003568A1 (es) 2015-04-24
SG10201509397SA (en) 2015-12-30
BR112014032560B1 (pt) 2022-04-26
US9150841B2 (en) 2015-10-06
JP2015521848A (ja) 2015-08-03
AU2018200710A1 (en) 2018-02-22
IL236343A0 (en) 2015-02-26
AU2016200460A1 (en) 2016-02-18
AU2018200710B2 (en) 2019-11-14
IL254560B (en) 2020-05-31
GT201400302A (es) 2019-05-22
MX2015000189A (es) 2015-04-08
CA2877521A1 (en) 2014-01-03

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