DOP2014000298A - Células para producir iduronato-2-sulfatasa recombinante - Google Patents

Células para producir iduronato-2-sulfatasa recombinante

Info

Publication number
DOP2014000298A
DOP2014000298A DO2014000298A DO2014000298A DOP2014000298A DO P2014000298 A DOP2014000298 A DO P2014000298A DO 2014000298 A DO2014000298 A DO 2014000298A DO 2014000298 A DO2014000298 A DO 2014000298A DO P2014000298 A DOP2014000298 A DO P2014000298A
Authority
DO
Dominican Republic
Prior art keywords
cells
recombinant
iduronate
produce
present
Prior art date
Application number
DO2014000298A
Other languages
English (en)
Inventor
Ferenc Boldog
Mike Heartlein
Original Assignee
Shire Human Genetic Therapies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shire Human Genetic Therapies filed Critical Shire Human Genetic Therapies
Publication of DOP2014000298A publication Critical patent/DOP2014000298A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
    • C12Y108/99—Oxidoreductases acting on sulfur groups as donors (1.8) with other acceptors (1.8.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/06—Sulfuric ester hydrolases (3.1.6)
    • C12Y301/06013—Iduronate-2-sulfatase (3.1.6.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención proporciona, entre otras cosas, métodos y composiciones para la producción de proteína I2S recombinante con mejor potencia y actividad usando células que coexpresan la proteínas I2S y FGE. En algunas modalidades, las células de acuerdo con la presente invención se modifican genéticamente para sobreexpresar simultáneamente las proteínas I2S y FGE recombinantes. Las células de acuerdo con la invención se pueden adaptar a varias condiciones de cultivo celular. En algunas modalidades, las células de la presente invención se pueden adaptar a un cultivo sin suero de suspensión a gran escala.
DO2014000298A 2012-06-29 2014-12-22 Células para producir iduronato-2-sulfatasa recombinante DOP2014000298A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261666719P 2012-06-29 2012-06-29

Publications (1)

Publication Number Publication Date
DOP2014000298A true DOP2014000298A (es) 2015-03-31

Family

ID=49778522

Family Applications (1)

Application Number Title Priority Date Filing Date
DO2014000298A DOP2014000298A (es) 2012-06-29 2014-12-22 Células para producir iduronato-2-sulfatasa recombinante

Country Status (27)

Country Link
US (3) US9150841B2 (es)
EP (1) EP2874649B1 (es)
JP (3) JP6067849B2 (es)
KR (1) KR101710487B1 (es)
CN (2) CN107267460A (es)
AU (3) AU2013282400B2 (es)
BR (1) BR112014032560B1 (es)
CA (1) CA2877521C (es)
CL (1) CL2014003568A1 (es)
CO (1) CO7240394A2 (es)
CR (1) CR20140586A (es)
DO (1) DOP2014000298A (es)
EA (2) EA202090805A1 (es)
ES (1) ES2718346T3 (es)
GT (1) GT201400302A (es)
HK (2) HK1209331A1 (es)
IL (3) IL236343A (es)
MX (2) MX336716B (es)
MY (2) MY157086A (es)
NZ (2) NZ734670A (es)
PE (1) PE20150957A1 (es)
PH (2) PH12014502868A1 (es)
SG (2) SG11201408702VA (es)
TR (1) TR201904435T4 (es)
UA (1) UA118955C2 (es)
WO (1) WO2014005019A2 (es)
ZA (1) ZA201409396B (es)

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Also Published As

Publication number Publication date
HK1209651A1 (en) 2016-04-08
IL236343A (en) 2017-10-31
CA2877521C (en) 2021-06-01
EP2874649A4 (en) 2016-05-04
IL236343A0 (en) 2015-02-26
US9719074B2 (en) 2017-08-01
NZ734670A (en) 2019-12-20
AU2016200460A1 (en) 2016-02-18
AU2018200710B2 (en) 2019-11-14
AU2018200710A1 (en) 2018-02-22
KR101710487B1 (ko) 2017-02-27
AU2013282400A1 (en) 2015-01-29
EA202090805A1 (ru) 2020-11-30
PH12016500011A1 (en) 2019-08-14
PH12014502868B1 (en) 2015-02-23
US20180010108A1 (en) 2018-01-11
IL254560B (en) 2020-05-31
ZA201409396B (en) 2018-05-30
KR20150030242A (ko) 2015-03-19
ES2718346T3 (es) 2019-07-01
IL274266B (en) 2021-04-29
EA035511B1 (ru) 2020-06-26
US20140004593A1 (en) 2014-01-02
MX366155B (es) 2019-06-28
EA201492183A1 (ru) 2015-06-30
CN104540517A (zh) 2015-04-22
BR112014032560B1 (pt) 2022-04-26
CN107267460A (zh) 2017-10-20
MY189314A (en) 2022-02-04
IL254560A0 (en) 2017-11-30
PE20150957A1 (es) 2015-07-15
BR112014032560A2 (pt) 2018-03-06
JP6067849B2 (ja) 2017-01-25
MX2015000189A (es) 2015-04-08
JP2018088933A (ja) 2018-06-14
GT201400302A (es) 2019-05-22
EP2874649A2 (en) 2015-05-27
AU2016200460B2 (en) 2017-11-02
JP6302499B2 (ja) 2018-03-28
JP2016105737A (ja) 2016-06-16
CL2014003568A1 (es) 2015-04-24
AU2013282400B2 (en) 2016-02-04
CR20140586A (es) 2015-04-06
MY157086A (en) 2016-04-19
SG11201408702VA (en) 2015-02-27
UA118955C2 (uk) 2019-04-10
US9150841B2 (en) 2015-10-06
CA2877521A1 (en) 2014-01-03
US20160053238A1 (en) 2016-02-25
EP2874649B1 (en) 2019-01-02
WO2014005019A3 (en) 2014-02-27
JP2015521848A (ja) 2015-08-03
CO7240394A2 (es) 2015-04-17
HK1209331A1 (en) 2016-04-01
TR201904435T4 (tr) 2019-05-21
NZ703137A (en) 2017-08-25
PH12014502868A1 (en) 2015-02-23
MX336716B (es) 2016-01-28
WO2014005019A2 (en) 2014-01-03
SG10201509397SA (en) 2015-12-30
JP6755272B2 (ja) 2020-09-16
IL274266A (en) 2020-06-30

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