MX336716B - Celulas para producir iduronato-2-sulfatasa recombinante. - Google Patents
Celulas para producir iduronato-2-sulfatasa recombinante.Info
- Publication number
- MX336716B MX336716B MX2015000189A MX2015000189A MX336716B MX 336716 B MX336716 B MX 336716B MX 2015000189 A MX2015000189 A MX 2015000189A MX 2015000189 A MX2015000189 A MX 2015000189A MX 336716 B MX336716 B MX 336716B
- Authority
- MX
- Mexico
- Prior art keywords
- cells
- sulfatase
- present
- producing recombinant
- adaptable
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y108/00—Oxidoreductases acting on sulfur groups as donors (1.8)
- C12Y108/99—Oxidoreductases acting on sulfur groups as donors (1.8) with other acceptors (1.8.99)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/06—Sulfuric ester hydrolases (3.1.6)
- C12Y301/06013—Iduronate-2-sulfatase (3.1.6.13)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/106—Plasmid DNA for vertebrates
- C12N2800/107—Plasmid DNA for vertebrates for mammalian
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Animal Behavior & Ethology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
La presente invención se refiere a una célula, caracterizada porque comprende un primer ácido nucleico que codifica una proteína iduronato-2-sulfatasa (I2S) que comprende una secuencia de aminoácidos al menos 90% idéntica a SEQ ID NO: 1; y un segundo ácido nucleico que codifica una enzima generadora de formilglicina (FGE) que comprende una secuencia de aminoácidos al menos 90% idéntica a SEQ ID NO: 5, en donde el primer y/o el segundo ácido nucleico son exógenos y en donde la célula, una vez cultivada en una condición de cultivo celular, produce proteína I2S que comprende al menos alrededor de 70% de conversión del residuo de cisteína que corresponde a Cys59 de SEQ ID NO: 1 en C?-formilglicina (FGly) y en donde el nivel de la proteína iduronato-2-sulfatasa expresada por la célula es de entre 0.3 y 10 veces mayor que el nivel de la enzima generadora de formilglicina (FGE) expresada por la célula.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261666719P | 2012-06-29 | 2012-06-29 | |
PCT/US2013/048571 WO2014005019A2 (en) | 2012-06-29 | 2013-06-28 | Cells for producing recombinant iduronate-2-sulfatase |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2015000189A MX2015000189A (es) | 2015-04-08 |
MX336716B true MX336716B (es) | 2016-01-28 |
Family
ID=49778522
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2016001229A MX366155B (es) | 2012-06-29 | 2013-06-28 | Celulas para producir iduronato-2-sulfatasa recombinante. |
MX2015000189A MX336716B (es) | 2012-06-29 | 2013-06-28 | Celulas para producir iduronato-2-sulfatasa recombinante. |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2016001229A MX366155B (es) | 2012-06-29 | 2013-06-28 | Celulas para producir iduronato-2-sulfatasa recombinante. |
Country Status (27)
Country | Link |
---|---|
US (3) | US9150841B2 (es) |
EP (1) | EP2874649B1 (es) |
JP (3) | JP6067849B2 (es) |
KR (1) | KR101710487B1 (es) |
CN (2) | CN107267460A (es) |
AU (3) | AU2013282400B2 (es) |
BR (1) | BR112014032560B1 (es) |
CA (1) | CA2877521C (es) |
CL (1) | CL2014003568A1 (es) |
CO (1) | CO7240394A2 (es) |
CR (1) | CR20140586A (es) |
DO (1) | DOP2014000298A (es) |
EA (2) | EA202090805A1 (es) |
ES (1) | ES2718346T3 (es) |
GT (1) | GT201400302A (es) |
HK (2) | HK1209331A1 (es) |
IL (3) | IL236343A (es) |
MX (2) | MX366155B (es) |
MY (2) | MY157086A (es) |
NZ (2) | NZ703137A (es) |
PE (1) | PE20150957A1 (es) |
PH (2) | PH12014502868A1 (es) |
SG (2) | SG10201509397SA (es) |
TR (1) | TR201904435T4 (es) |
UA (1) | UA118955C2 (es) |
WO (1) | WO2014005019A2 (es) |
ZA (1) | ZA201409396B (es) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2012019168A2 (en) | 2010-08-06 | 2012-02-09 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
CA2821992A1 (en) | 2010-10-01 | 2012-04-05 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
CA2831613A1 (en) | 2011-03-31 | 2012-10-04 | Moderna Therapeutics, Inc. | Delivery and formulation of engineered nucleic acids |
US20160145589A1 (en) | 2011-06-24 | 2016-05-26 | Green Cross Corporation | Composition and formulation comprising recombinant human iduronate-2-sulfatase and preparation method thereof |
DE12858350T1 (de) | 2011-12-16 | 2021-10-07 | Modernatx, Inc. | Modifizierte mrna zusammensetzungen |
US9572897B2 (en) | 2012-04-02 | 2017-02-21 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
US10501512B2 (en) | 2012-04-02 | 2019-12-10 | Modernatx, Inc. | Modified polynucleotides |
EP3501550A1 (en) | 2012-04-02 | 2019-06-26 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of proteins associated with human disease |
US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
CA2872476C (en) * | 2012-05-02 | 2020-07-07 | Life Technologies Corporation | High yield transient expression in mammalian cells using unique pairing of high density growth and transfection medium and expression enhancers |
US20140004097A1 (en) * | 2012-06-29 | 2014-01-02 | Shire Human Genetic Therapies, Inc. | Method of producing recombinant iduronate-2-sulfatase |
KR101380740B1 (ko) | 2012-06-29 | 2014-04-11 | 쉐어 휴먼 제네텍 세러피스, 인코포레이티드 | 이듀로네이트-2-설파타제의 정제 |
US9150841B2 (en) * | 2012-06-29 | 2015-10-06 | Shire Human Genetic Therapies, Inc. | Cells for producing recombinant iduronate-2-sulfatase |
US9512456B2 (en) | 2012-08-14 | 2016-12-06 | Modernatx, Inc. | Enzymes and polymerases for the synthesis of RNA |
US9597380B2 (en) | 2012-11-26 | 2017-03-21 | Modernatx, Inc. | Terminally modified RNA |
WO2014159813A1 (en) | 2013-03-13 | 2014-10-02 | Moderna Therapeutics, Inc. | Long-lived polynucleotide molecules |
EP2971010B1 (en) | 2013-03-14 | 2020-06-10 | ModernaTX, Inc. | Formulation and delivery of modified nucleoside, nucleotide, and nucleic acid compositions |
US10023626B2 (en) | 2013-09-30 | 2018-07-17 | Modernatx, Inc. | Polynucleotides encoding immune modulating polypeptides |
US9550972B2 (en) | 2014-09-29 | 2017-01-24 | General Electric Company | Devices, systems and methods for automated cell culturing |
EP3101125A1 (en) * | 2015-06-05 | 2016-12-07 | Laboratorios Del Dr. Esteve, S.A. | Adenoassociated virus vectors for the treatment of mucopolysaccharidoses |
US10849920B2 (en) | 2015-10-05 | 2020-12-01 | Modernatx, Inc. | Methods for therapeutic administration of messenger ribonucleic acid drugs |
JP2021500857A (ja) | 2017-10-02 | 2021-01-14 | デナリ セラピューティクス インコーポレイテッドDenali Therapeutics Inc. | 酵素補充療法用酵素を含む融合タンパク質 |
JP7060235B2 (ja) * | 2018-05-18 | 2022-04-26 | 国立大学法人広島大学 | 変異型イズロン酸-2-スルファターゼの機能回復薬剤のスクリーニング方法 |
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DK6488D0 (da) | 1988-01-07 | 1988-01-07 | Novo Industri As | Enzymer |
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CA2711590C (en) | 2008-01-18 | 2021-03-23 | Biomarin Pharmaceutical Inc. | Manufacture of active highly phosphorylated human lysosomal sulfatase enzymes and uses thereof |
HUE044865T2 (hu) | 2009-10-09 | 2019-11-28 | Armagen Inc | Eljárások és készítmények a központi idegrendszerben iduronát-2-szulfatáz-aktivitás növelésére |
CN105664811B (zh) * | 2009-10-09 | 2018-10-09 | 蓝立方知识产权公司 | 生产氯化和/或氟化丙烯和高级烯烃的绝热活塞流反应器及方法 |
WO2011108451A1 (ja) | 2010-03-01 | 2011-09-09 | 日本ケミカルリサーチ株式会社 | 遺伝子ノックアウト細胞を用いた組換え体リソソーム酵素の製造方法 |
CN103179980B (zh) | 2010-06-25 | 2016-09-28 | 夏尔人类遗传性治疗公司 | 艾杜糖醛酸-2-硫酸酯酶的cns递送的方法和组合物 |
WO2012101671A1 (en) | 2011-01-25 | 2012-08-02 | Jcr Pharmaceuticals Co., Ltd. | Method for production of human recombinant iduronate 2-sulfatase |
KR101158673B1 (ko) | 2011-06-24 | 2012-07-03 | 주식회사 지씨바이오 | 재조합 인간 이듀로네이트-2-설파타아제를 포함하는 조성물, 제제 및 이의 제조방법 |
US20140004097A1 (en) | 2012-06-29 | 2014-01-02 | Shire Human Genetic Therapies, Inc. | Method of producing recombinant iduronate-2-sulfatase |
US9150841B2 (en) | 2012-06-29 | 2015-10-06 | Shire Human Genetic Therapies, Inc. | Cells for producing recombinant iduronate-2-sulfatase |
KR101380740B1 (ko) | 2012-06-29 | 2014-04-11 | 쉐어 휴먼 제네텍 세러피스, 인코포레이티드 | 이듀로네이트-2-설파타제의 정제 |
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2013
- 2013-03-14 US US13/829,780 patent/US9150841B2/en active Active
- 2013-06-28 UA UAA201413829A patent/UA118955C2/uk unknown
- 2013-06-28 SG SG10201509397SA patent/SG10201509397SA/en unknown
- 2013-06-28 JP JP2015520569A patent/JP6067849B2/ja active Active
- 2013-06-28 EA EA202090805A patent/EA202090805A1/ru unknown
- 2013-06-28 BR BR112014032560-0A patent/BR112014032560B1/pt active IP Right Grant
- 2013-06-28 NZ NZ703137A patent/NZ703137A/en unknown
- 2013-06-28 PE PE2014002538A patent/PE20150957A1/es not_active Application Discontinuation
- 2013-06-28 AU AU2013282400A patent/AU2013282400B2/en active Active
- 2013-06-28 MX MX2016001229A patent/MX366155B/es unknown
- 2013-06-28 ES ES13810018T patent/ES2718346T3/es active Active
- 2013-06-28 CN CN201710300757.6A patent/CN107267460A/zh active Pending
- 2013-06-28 CN CN201380037937.XA patent/CN104540517A/zh active Pending
- 2013-06-28 MY MYPI2014003485A patent/MY157086A/en unknown
- 2013-06-28 KR KR1020157000841A patent/KR101710487B1/ko active IP Right Grant
- 2013-06-28 SG SG11201408702VA patent/SG11201408702VA/en unknown
- 2013-06-28 MX MX2015000189A patent/MX336716B/es unknown
- 2013-06-28 TR TR2019/04435T patent/TR201904435T4/tr unknown
- 2013-06-28 CA CA2877521A patent/CA2877521C/en active Active
- 2013-06-28 WO PCT/US2013/048571 patent/WO2014005019A2/en active Application Filing
- 2013-06-28 NZ NZ734670A patent/NZ734670A/en unknown
- 2013-06-28 MY MYPI2016000156A patent/MY189314A/en unknown
- 2013-06-28 EP EP13810018.5A patent/EP2874649B1/en active Active
- 2013-06-28 EA EA201492183A patent/EA035511B1/ru not_active IP Right Cessation
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2014
- 2014-12-17 CR CR20140586A patent/CR20140586A/es unknown
- 2014-12-18 IL IL236343A patent/IL236343A/en active IP Right Grant
- 2014-12-19 ZA ZA2014/09396A patent/ZA201409396B/en unknown
- 2014-12-22 PH PH12014502868A patent/PH12014502868A1/en unknown
- 2014-12-22 DO DO2014000298A patent/DOP2014000298A/es unknown
- 2014-12-26 CO CO14284192A patent/CO7240394A2/es unknown
- 2014-12-29 CL CL2014003568A patent/CL2014003568A1/es unknown
- 2014-12-29 GT GT201400302A patent/GT201400302A/es unknown
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2015
- 2015-08-21 US US14/832,728 patent/US9719074B2/en active Active
- 2015-10-14 HK HK15110031.3A patent/HK1209331A1/xx unknown
- 2015-10-28 HK HK15110644.2A patent/HK1209651A1/xx unknown
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2016
- 2016-01-04 PH PH12016500011A patent/PH12016500011A1/en unknown
- 2016-01-27 AU AU2016200460A patent/AU2016200460B2/en active Active
- 2016-03-16 JP JP2016052380A patent/JP6302499B2/ja active Active
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2017
- 2017-07-14 US US15/650,536 patent/US20180010108A1/en not_active Abandoned
- 2017-09-18 IL IL254560A patent/IL254560B/en active IP Right Grant
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2018
- 2018-01-30 AU AU2018200710A patent/AU2018200710B2/en active Active
- 2018-03-02 JP JP2018037376A patent/JP6755272B2/ja active Active
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2020
- 2020-04-27 IL IL274266A patent/IL274266B/en active IP Right Grant
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