EA201991976A1 - Метаболически оптимизированная клеточная культура - Google Patents

Метаболически оптимизированная клеточная культура

Info

Publication number
EA201991976A1
EA201991976A1 EA201991976A EA201991976A EA201991976A1 EA 201991976 A1 EA201991976 A1 EA 201991976A1 EA 201991976 A EA201991976 A EA 201991976A EA 201991976 A EA201991976 A EA 201991976A EA 201991976 A1 EA201991976 A1 EA 201991976A1
Authority
EA
Eurasian Patent Office
Prior art keywords
cell culture
proteins
optimized cell
present
polypeptides
Prior art date
Application number
EA201991976A
Other languages
English (en)
Inventor
Шон Лоуренс
Энн Ким
Эми Джонсон
Original Assignee
Регенерон Фармасьютикалз, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52813657&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA201991976(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Регенерон Фармасьютикалз, Инк. filed Critical Регенерон Фармасьютикалз, Инк.
Publication of EA201991976A1 publication Critical patent/EA201991976A1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Предусмотрен улучшенный способ крупномасштабного получения белков и/или полипептидов в клеточной культуре. Согласно настоящему изобретению способ предусматривает культивирование клеток, в которых произошел метаболический сдвиг. Применение такого способа или системы обеспечивает высокие уровни производства белка или полипептида и снижает накопление такого нежелательного отхода метаболизма, как лактат. Белки и полипептиды, экспрессированные согласно настоящему изобретению, могут успешно применяться в получении фармацевтических, иммуногенных или других коммерческих биологических композиций, таких как антитела.
EA201991976A 2013-10-11 2014-10-10 Метаболически оптимизированная клеточная культура EA201991976A1 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361889815P 2013-10-11 2013-10-11

Publications (1)

Publication Number Publication Date
EA201991976A1 true EA201991976A1 (ru) 2020-04-02

Family

ID=52813657

Family Applications (2)

Application Number Title Priority Date Filing Date
EA201690600A EA033783B1 (ru) 2013-10-11 2014-10-10 Метаболически оптимизированная клеточная культура
EA201991976A EA201991976A1 (ru) 2013-10-11 2014-10-10 Метаболически оптимизированная клеточная культура

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EA201690600A EA033783B1 (ru) 2013-10-11 2014-10-10 Метаболически оптимизированная клеточная культура

Country Status (18)

Country Link
US (2) US20160244725A1 (ru)
EP (3) EP3351620B1 (ru)
JP (3) JP6663854B2 (ru)
KR (4) KR102358807B1 (ru)
CN (2) CN114717196A (ru)
AU (2) AU2014331776B2 (ru)
CA (1) CA2926049C (ru)
DK (2) DK3055409T3 (ru)
EA (2) EA033783B1 (ru)
ES (2) ES2678945T3 (ru)
HK (2) HK1223122A1 (ru)
HU (2) HUE039551T2 (ru)
IL (2) IL244511A0 (ru)
MX (2) MX2016004564A (ru)
PL (2) PL3351620T3 (ru)
SG (2) SG10201800772TA (ru)
WO (1) WO2015054554A1 (ru)
ZA (1) ZA201601550B (ru)

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JP7260011B2 (ja) * 2017-03-31 2023-04-18 株式会社三洋物産 遊技機
JP7260018B2 (ja) * 2017-03-31 2023-04-18 株式会社三洋物産 遊技機
JP7260020B2 (ja) * 2017-03-31 2023-04-18 株式会社三洋物産 遊技機
JP7260009B2 (ja) * 2017-04-27 2023-04-18 株式会社三洋物産 遊技機
JP7310952B2 (ja) * 2017-04-27 2023-07-19 株式会社三洋物産 遊技機
JP7260013B2 (ja) * 2017-04-27 2023-04-18 株式会社三洋物産 遊技機
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Also Published As

Publication number Publication date
KR102610425B1 (ko) 2023-12-06
KR20210046835A (ko) 2021-04-28
HK1253275A1 (zh) 2019-06-14
AU2014331776B2 (en) 2020-10-22
KR102358807B1 (ko) 2022-02-08
CN114717196A (zh) 2022-07-08
AU2014331776A1 (en) 2016-03-24
IL244511A0 (en) 2016-04-21
MX2020011334A (es) 2021-07-30
HK1223122A1 (zh) 2017-07-21
ZA201601550B (en) 2023-05-31
SG11201601681QA (en) 2016-04-28
EP3351620A1 (en) 2018-07-25
CN105637085A (zh) 2016-06-01
IL262833A (en) 2018-12-31
EA201690600A1 (ru) 2016-09-30
AU2021200193B2 (en) 2023-08-24
JP7377243B2 (ja) 2023-11-09
HUE039551T2 (hu) 2019-01-28
MX2016004564A (es) 2016-07-12
AU2021200193A1 (en) 2021-03-18
KR20160062032A (ko) 2016-06-01
SG10201800772TA (en) 2018-03-28
KR20230038324A (ko) 2023-03-17
JP6663854B2 (ja) 2020-03-13
ES2678945T3 (es) 2018-08-21
JP2016532457A (ja) 2016-10-20
KR102243980B1 (ko) 2021-04-26
JP2020072760A (ja) 2020-05-14
EP3055409A1 (en) 2016-08-17
JP6967620B2 (ja) 2021-11-17
PL3055409T3 (pl) 2018-10-31
EA033783B1 (ru) 2019-11-25
US20210087535A1 (en) 2021-03-25
WO2015054554A1 (en) 2015-04-16
KR20220020415A (ko) 2022-02-18
DK3055409T3 (en) 2018-07-30
DK3351620T3 (da) 2020-10-26
HUE051049T2 (hu) 2021-01-28
EP3351620B1 (en) 2020-09-02
KR102510238B1 (ko) 2023-03-16
US20160244725A1 (en) 2016-08-25
PL3351620T3 (pl) 2021-03-08
EP3055409B1 (en) 2018-04-25
CA2926049A1 (en) 2015-04-16
ES2833471T3 (es) 2021-06-15
EP3055409A4 (en) 2016-09-28
CA2926049C (en) 2023-09-05
JP2022009453A (ja) 2022-01-14
EP3812453A1 (en) 2021-04-28

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