PL2150617T3 - Wzmocniona ekspresja i regiony stabilności - Google Patents

Wzmocniona ekspresja i regiony stabilności

Info

Publication number
PL2150617T3
PL2150617T3 PL08756678T PL08756678T PL2150617T3 PL 2150617 T3 PL2150617 T3 PL 2150617T3 PL 08756678 T PL08756678 T PL 08756678T PL 08756678 T PL08756678 T PL 08756678T PL 2150617 T3 PL2150617 T3 PL 2150617T3
Authority
PL
Poland
Prior art keywords
enhanced expression
stability regions
regions
stability
enhanced
Prior art date
Application number
PL08756678T
Other languages
English (en)
Inventor
Gang Chen
Robert Babb
James P Fandl
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=39736874&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL2150617(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of PL2150617T3 publication Critical patent/PL2150617T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
PL08756678T 2007-06-04 2008-06-04 Wzmocniona ekspresja i regiony stabilności PL2150617T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US93321307P 2007-06-04 2007-06-04
PCT/US2008/065733 WO2008151219A1 (en) 2007-06-04 2008-06-04 Enhanced expression and stability regions
EP08756678.2A EP2150617B1 (en) 2007-06-04 2008-06-04 Enhanced expression and stability regions

Publications (1)

Publication Number Publication Date
PL2150617T3 true PL2150617T3 (pl) 2015-04-30

Family

ID=39736874

Family Applications (1)

Application Number Title Priority Date Filing Date
PL08756678T PL2150617T3 (pl) 2007-06-04 2008-06-04 Wzmocniona ekspresja i regiony stabilności

Country Status (6)

Country Link
US (6) US7771997B2 (pl)
EP (1) EP2150617B1 (pl)
DK (1) DK2150617T3 (pl)
ES (1) ES2522615T3 (pl)
PL (1) PL2150617T3 (pl)
WO (1) WO2008151219A1 (pl)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2522615T3 (es) * 2007-06-04 2014-11-17 Regeneron Pharmaceuticals, Inc. Regiones de expresión y estabilidad potenciadas
BRPI1010035A2 (pt) 2009-06-02 2015-08-25 Regeneron Pharma Células deficientes em fucosilação
TWI641687B (zh) 2012-05-29 2018-11-21 美商再生元醫藥公司 生產細胞株增強子
EP2925867B1 (en) 2012-11-30 2018-06-27 Larix Biosciences LLC A novel cell line screening method
TWI635098B (zh) 2013-02-01 2018-09-11 再生元醫藥公司 含嵌合恆定區之抗體
JP6525951B2 (ja) 2013-03-14 2019-06-05 リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． アペリン融合タンパク質およびその使用
AR095196A1 (es) 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
KR102358807B1 (ko) 2013-10-11 2022-02-08 리제너론 파마슈티칼스 인코포레이티드 대사적으로 최적화된 세포 배양
CN103642560A (zh) * 2013-10-14 2014-03-19 无锡通用钢绳有限公司 一种钢丝绳表面脂及其制备方法
AR102333A1 (es) 2014-08-19 2017-02-22 Regeneron Pharma Selectividad eficiente de proteínas recombinadas
CA2965495C (en) * 2014-10-23 2022-05-24 Regeneron Pharmaceuticals, Inc. Cho integration sites and uses thereof
TW201702380A (zh) * 2015-02-27 2017-01-16 再生元醫藥公司 宿主細胞蛋白質修飾
TWI804917B (zh) 2015-03-27 2023-06-11 美商再生元醫藥公司 偵測生物污染物之組成物及方法
JP7240812B2 (ja) 2015-05-12 2023-03-16 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 多量体タンパク質の純度の決定
US11191844B2 (en) 2015-07-06 2021-12-07 Regeneran Pharmaceuticals, Inc. Multispecific antigen-binding molecules and uses thereof
TWI899515B (zh) 2015-08-04 2025-10-01 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法
SG11201807881VA (en) * 2016-04-20 2018-10-30 Regeneron Pharma Compositions and methods for making antibodies based on use of an expression-enhancing locus
KR102906454B1 (ko) * 2016-04-20 2026-01-02 리제너론 파마슈티칼스 인코포레이티드 발현 강화 유전자좌의 사용에 기초하여 항체를 만들기 위한 조성물 및 방법
EP3448891A1 (en) 2016-04-28 2019-03-06 Regeneron Pharmaceuticals, Inc. Methods of making multispecific antigen-binding molecules
US20200317738A1 (en) 2016-05-26 2020-10-08 University College Cork - National University Of Ireland, Cork Methods for increasing proliferation of mammalian cells
WO2017203051A1 (en) 2016-05-26 2017-11-30 University College Cork - National University Of Ireland, Cork An engineered gram positive bacterium
HUE066064T2 (hu) 2016-08-18 2024-07-28 Regeneron Pharma Fehérje önasszociációs képességének meghatározására szolgáló assay koncentrációfüggõ öninterakciós nanorészecske-spektroszkópia segítségével
WO2018039499A1 (en) 2016-08-24 2018-03-01 Regeneron Pharmaceuticals, Inc. Host cell protein modification
CN110022855B (zh) 2016-10-07 2024-07-16 瑞泽恩制药公司 室温稳定的冻干蛋白质
JP7637470B2 (ja) 2017-02-17 2025-02-28 ロンザ リミテッド アデノ随伴ウイルスを生産するための哺乳動物細胞
JP7467119B2 (ja) 2017-02-17 2024-04-15 ロンザ リミテッド 発現困難タンパク質のための多部位ｓｓｉ細胞
EP3635009B1 (en) 2017-06-07 2026-02-25 Regeneron Pharmaceuticals, Inc. Compositions and methods for internalizing enzymes
CN114075269A (zh) 2017-07-06 2022-02-22 菲仕兰坎皮纳荷兰私人有限公司 用于制备糖蛋白的细胞培养工艺
EP3673044A1 (en) 2017-08-25 2020-07-01 University College Cork-National University of Ireland, Cork Bifidobacterium longum for treating obesity and weight management
JP2020534812A (ja) 2017-09-08 2020-12-03 ライフ テクノロジーズ コーポレイション 改良された相同組換えおよびその組成物のための方法
CN111315890A (zh) 2017-09-08 2020-06-19 生命技术公司 提高同源重组的方法和其组合物
US20200345051A1 (en) 2017-11-24 2020-11-05 University College Cork, National University Of Ireland, Cork A composition comprising a cohort of bacteria
IL278244B2 (en) 2018-04-30 2026-01-01 Regeneron Pharma Antibodies and bispecific antigen-binding molecules that bind HER2 and/or APLP2, conjugates and uses thereof
MA52626A (fr) 2018-05-17 2021-03-24 Regeneron Pharma Anticorps anti-cd63, conjugués et leurs utilisations
EP3604507A1 (en) 2018-07-30 2020-02-05 University College Cork-National University of Ireland, Cork An omega-transaminase enzyme
CA3134705A1 (en) 2019-04-02 2020-10-08 Chugai Seiyaku Kabushiki Kaisha Method of introducing target-specific foreign gene
JP2022545265A (ja) 2019-08-20 2022-10-26 ニューリタス リミテッド 筋萎縮を治療するためのペプチド
EP3783012A1 (en) 2019-08-20 2021-02-24 Nuritas Limited An antimicrobial peptide
CN114980913B (zh) 2019-10-22 2025-09-30 努里塔斯有限公司 非酒精性脂肪性肝病的治疗
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
EP4070670A1 (en) 2021-04-08 2022-10-12 University College Cork-National University of Ireland Cork Lacticaseibacillus paracasei em025-11 and uses thereof
AU2022259536A1 (en) 2021-04-14 2023-11-09 University College Cork - National University Of Ireland, Cork Treatment of cerebrovascular events and neurological disorders
JP2024514194A (ja) 2021-04-14 2024-03-28 ユニバーシティ・カレッジ・コークーナショナル・ユニバーシティ・オブ・アイルランド，コーク 変形性関節症の治療に使用するためのｐｓｇ１
CN118076894A (zh) 2021-10-07 2024-05-24 里珍纳龙药品有限公司 pH建模和控制的系统及方法
KR20240090312A (ko) 2021-10-07 2024-06-21 리제너론 파아마슈티컬스, 인크. Ph 미터 보정 및 교정
KR20240101596A (ko) 2021-10-18 2024-07-02 리제너론 파마슈티칼스 인코포레이티드 폴리뉴클레오티드의 제어된 전사
JP2024537896A (ja) 2021-10-18 2024-10-16 リジェネロン・ファーマシューティカルズ・インコーポレイテッド アデノウイルス随伴ウイルスポリヌクレオチドを含む真核細胞
US20230287460A1 (en) 2021-10-18 2023-09-14 Regeneron Pharmaceuticals, Inc. Mammalian cells comprising integrated cas9 genes to produce stable integration sites, and mammalian cells comprising stable integration sites and other sites
CN120379687A (zh) 2022-10-14 2025-07-25 爱尔兰国立科克大学 胎盘表达的蛋白用于治疗肌腱损伤
WO2025133254A2 (en) 2023-12-20 2025-06-26 Nuritas Limited Angiotensin-converting enzyme (ace) inhibiting peptides.
WO2025133189A1 (en) 2023-12-20 2025-06-26 Nuritas Limited Sleep promoting peptides and the use thereof
WO2026050618A1 (en) 2024-08-29 2026-03-05 Regeneron Pharmaceuticals, Inc. Polynucleotide constructs and cell lines for recombinant aav production

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4634665A (en) 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
US6689606B2 (en) * 1998-07-21 2004-02-10 M.L. Laboratories Plc Polynucleotide
AU2002254529A1 (en) * 2001-04-04 2002-10-21 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Nucleic acids for transgene expression
FR2910490B1 (fr) 2006-12-20 2012-10-26 Lab Francais Du Fractionnement Lignee cellulaire a forte activite transcriptionnelle pour la production de proteines, notamment therapeutiques
ES2522615T3 (es) 2007-06-04 2014-11-17 Regeneron Pharmaceuticals, Inc. Regiones de expresión y estabilidad potenciadas

Also Published As

Publication number Publication date
US20180327777A1 (en) 2018-11-15
EP2150617A1 (en) 2010-02-10
US9562238B2 (en) 2017-02-07
US10415055B2 (en) 2019-09-17
US20170096681A1 (en) 2017-04-06
US8389239B2 (en) 2013-03-05
WO2008151219A1 (en) 2008-12-11
US20130130372A1 (en) 2013-05-23
US9222106B2 (en) 2015-12-29
ES2522615T3 (es) 2014-11-17
US20100291626A1 (en) 2010-11-18
EP2150617B1 (en) 2014-10-22
US9932605B2 (en) 2018-04-03
US20090124005A1 (en) 2009-05-14
US20160097059A1 (en) 2016-04-07
US7771997B2 (en) 2010-08-10
DK2150617T3 (en) 2015-01-12

Similar Documents

Publication Publication Date Title
PL2150617T3 (pl) Wzmocniona ekspresja i regiony stabilności
IL205571A0 (en) 4-pyrazolyl-n-arylpyrimidin-2-amines and 4-pyrazolyl-n-heteroarylpyrimidin
EP2185692A4 (en) HCO32 AND HCO27 AND RELATED EXAMPLES
IL210804A (en) Benzylidenehydrazides and their uses
EP2069496A4 (en) SELEX AND PHOTOSELEX ENHANCED
IL211042A0 (en) Imidazopyridin-2-one derivatives and uses thereof
ZA200810323B (en) Fkbp-l and uses thereof
ZA201103635B (en) Azaquinolinone derivatives and uses thereof
PT2114900T (pt) Compostos à base de tiopirimidina e as suas utilizações
PL3222615T3 (pl) Środki wiążące psma i ich zastosowania
IL211742A0 (en) Frizzled-binding agents and uses thereof
SI2064327T1 (sl) DBAIT in njegova uporaba
GB0808575D0 (en) Compilign and linking
GB0921531D0 (en) Playpen and related playpen set
GB0822154D0 (en) Recepatacle and support
EP2291377A4 (en) IMIDAZOPYRIMIDINONE AND ITS USES
ZA200903906B (en) Carboranylporphyrins and uses thereof
ZA201103456B (en) Leukolectins and uses thereof
IL208771A0 (en) Iminopyridine derivatives and use thereof
PL2247601T3 (pl) Tiazopirymidynony i ich zastosowanie
ZA201000357B (en) 2-Azetidinemethaneamines and 2-pyrrolidinemethaneamines as Taar-Ligands
ZA201001402B (en) Mouse and stay thereof
EP2274294A4 (en) 5-DIMETHOXY FUMAGILLOL AND ITS DERIVATIVES
IL206951A0 (en) 3-alkyl-piperazine derivatives and uses thereof
GB0813359D0 (en) Halfin and/or platerfin