MX2017005306A - Novedosos sitios de integracion en celulas cho y usos de estos. - Google Patents

Novedosos sitios de integracion en celulas cho y usos de estos.

Info

Publication number
MX2017005306A
MX2017005306A MX2017005306A MX2017005306A MX2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A
Authority
MX
Mexico
Prior art keywords
expression
integration
cho cells
new sites
eukaryotic
Prior art date
Application number
MX2017005306A
Other languages
English (en)
Inventor
Chen Gang
Burakov Darya
Shen Ying
P Fandl James
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=54602002&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2017005306(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2017005306A publication Critical patent/MX2017005306A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Saccharide Compounds (AREA)

Abstract

Se proporcionan secuencias de nucleótidos potenciadoras de la expresión para sistemas de expresión en eucariotas que permiten la expresión estable y aumentada de proteínas recombinantes en células eucariotas. Se proporcionan sitios de integración genómica que proporcionan expresión aumentada y métodos de uso de estos para la expresión de un gen de interés en una célula eucariota. Se proporcionan loci cromosómicos, secuencia, y vectores para la expresión estable y aumentada de genes en células eucariotas.
MX2017005306A 2014-10-23 2015-10-21 Novedosos sitios de integracion en celulas cho y usos de estos. MX2017005306A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462067774P 2014-10-23 2014-10-23
PCT/US2015/056653 WO2016064999A1 (en) 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof

Publications (1)

Publication Number Publication Date
MX2017005306A true MX2017005306A (es) 2018-01-09

Family

ID=54602002

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017005306A MX2017005306A (es) 2014-10-23 2015-10-21 Novedosos sitios de integracion en celulas cho y usos de estos.

Country Status (16)

Country Link
US (5) US9816110B2 (es)
EP (1) EP3209785B1 (es)
JP (1) JP6668340B2 (es)
KR (1) KR102243243B1 (es)
CN (1) CN107109434A (es)
AR (1) AR102420A1 (es)
AU (1) AU2015335921B2 (es)
BR (1) BR112017008022A2 (es)
CA (1) CA2965495C (es)
EA (1) EA037255B1 (es)
IL (1) IL251674B (es)
MX (1) MX2017005306A (es)
MY (1) MY184103A (es)
SG (1) SG11201703149RA (es)
TW (2) TWI780847B (es)
WO (1) WO2016064999A1 (es)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2965495C (en) 2014-10-23 2022-05-24 Regeneron Pharmaceuticals, Inc. Cho integration sites and uses thereof
US11649449B2 (en) 2015-09-03 2023-05-16 CHO Plus, Inc. Hybrid cell lines for high level production of a target protein
US10329594B1 (en) 2015-09-03 2019-06-25 CHO Plus, Inc. Cell lines for high level production of protein-based pharmaceuticals
SG11201807881VA (en) * 2016-04-20 2018-10-30 Regeneron Pharma Compositions and methods for making antibodies based on use of an expression-enhancing locus
KR102906454B1 (ko) * 2016-04-20 2026-01-02 리제너론 파마슈티칼스 인코포레이티드 발현 강화 유전자좌의 사용에 기초하여 항체를 만들기 위한 조성물 및 방법
WO2018039499A1 (en) 2016-08-24 2018-03-01 Regeneron Pharmaceuticals, Inc. Host cell protein modification
US11499139B2 (en) 2016-12-20 2022-11-15 Development Center For Biotechnology Targeted integration sites in Chinese hamster ovary cell genome
US20210309988A1 (en) * 2017-02-07 2021-10-07 Sigma-Aldrich Co. Llc Stable targeted integration
JP7637470B2 (ja) * 2017-02-17 2025-02-28 ロンザ リミテッド アデノ随伴ウイルスを生産するための哺乳動物細胞
CN110431227A (zh) * 2017-03-19 2019-11-08 应用干细胞有限公司 新型整合位点及其用途
SG11202000966XA (en) * 2017-08-11 2020-02-27 Boehringer Ingelheim Int Integration sites in CHO cells
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
IL275462B2 (en) * 2017-12-22 2026-01-01 Genentech Inc Targeted integration of nucleic acids
WO2019126562A1 (en) * 2017-12-22 2019-06-27 Bio-Rad Laboratories, Inc. Controlling phenotype of organisms with crispr/cas gene targeting
WO2020084034A1 (en) 2018-10-26 2020-04-30 F. Hoffmann-La Roche Ag Multispecific antibody screening method using recombinase mediated cassette exchange
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109295092B (zh) * 2018-10-30 2021-06-29 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
SG11202106523SA (en) * 2018-12-21 2021-07-29 Genentech Inc Targeted integration of nucleic acids
CA3134705A1 (en) 2019-04-02 2020-10-08 Chugai Seiyaku Kabushiki Kaisha Method of introducing target-specific foreign gene
KR20220025806A (ko) 2019-06-26 2022-03-03 제넨테크, 인크. 핵산의 무작위 구성 표적화 통합
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
AU2021283272A1 (en) * 2020-06-02 2023-01-19 Catalent Pharma Solutions, Llc Cell lines with multiple docks for gene insertion
IL299120A (en) * 2020-06-24 2023-02-01 Genentech Inc A targeted combination of nucleic acids
WO2022079083A1 (en) 2020-10-15 2022-04-21 F. Hoffmann-La Roche Ag Nucleic acid constructs for va rna transcription
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
US20230287460A1 (en) 2021-10-18 2023-09-14 Regeneron Pharmaceuticals, Inc. Mammalian cells comprising integrated cas9 genes to produce stable integration sites, and mammalian cells comprising stable integration sites and other sites
JP2024537896A (ja) 2021-10-18 2024-10-16 リジェネロン・ファーマシューティカルズ・インコーポレイテッド アデノウイルス随伴ウイルスポリヌクレオチドを含む真核細胞
KR20240101596A (ko) 2021-10-18 2024-07-02 리제너론 파마슈티칼스 인코포레이티드 폴리뉴클레오티드의 제어된 전사
KR20240139051A (ko) * 2022-01-28 2024-09-20 더 트러스티스 오브 콜롬비아 유니버시티 인 더 시티 오브 뉴욕 이종이식을 위한 유전 변형
CN117305334B (zh) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
WO2024107451A1 (en) 2022-11-18 2024-05-23 Regeneron Pharmaceuticals, Inc. Methods for detecting and evaluating viruses and virus-like particles
CN118726475B (zh) * 2023-03-28 2025-07-08 深圳太力生物技术有限责任公司 核酸、重组载体、靶向整合细胞、基因表达方法和应用
WO2024211287A1 (en) * 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
US20250122482A1 (en) 2023-09-07 2025-04-17 Regeneron Pharmaceuticals, Inc. Production and purification of covalently surface modified adeno-associated virus
US20250163468A1 (en) 2023-11-21 2025-05-22 Regeneron Pharmaceuticals, Inc. Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus
WO2026050618A1 (en) 2024-08-29 2026-03-05 Regeneron Pharmaceuticals, Inc. Polynucleotide constructs and cell lines for recombinant aav production

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4634665A (en) 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
US6689606B2 (en) 1998-07-21 2004-02-10 M.L. Laboratories Plc Polynucleotide
US6521419B1 (en) * 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
AU2002254529A1 (en) 2001-04-04 2002-10-21 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Nucleic acids for transgene expression
US20040045043A1 (en) * 2002-05-20 2004-03-04 Finney Robert E. Compositions and methods for generating conditional knockouts
US8673589B2 (en) 2002-05-29 2014-03-18 Regeneron Pharmaceuticals, Inc. Inducible eukaryotic expression system
ES2522615T3 (es) * 2007-06-04 2014-11-17 Regeneron Pharmaceuticals, Inc. Regiones de expresión y estabilidad potenciadas
JP5681114B2 (ja) * 2008-12-04 2015-03-04 サンガモ バイオサイエンシーズ， インコーポレイテッド 亜鉛フィンガーヌクレアーゼを使用したラットのゲノム編集
SG10201914098YA (en) 2011-04-05 2020-02-27 Scripps Research Inst Chromosomal landing pads and related uses
JP6072771B2 (ja) 2011-04-20 2017-02-01 メディミューン，エルエルシー Ｂ７−ｈ１およびｐｄ−１に結合する抗体およびその他の分子
WO2012167192A2 (en) * 2011-06-01 2012-12-06 Precision Biosciences, Inc. Methods and products for producing engineered mammalian cell lines with amplified transgenes
CA2852955C (en) * 2011-10-27 2021-02-16 Sangamo Biosciences, Inc. Methods and compositions for modification of the hprt locus
CA2871524C (en) 2012-05-07 2021-07-27 Sangamo Biosciences, Inc. Methods and compositions for nuclease-mediated targeted integration of transgenes
CA2965495C (en) 2014-10-23 2022-05-24 Regeneron Pharmaceuticals, Inc. Cho integration sites and uses thereof

Also Published As

Publication number Publication date
IL251674B (en) 2020-10-29
IL251674A0 (en) 2017-06-29
TW201629229A (zh) 2016-08-16
US20180030480A1 (en) 2018-02-01
US20210171984A1 (en) 2021-06-10
JP2017535258A (ja) 2017-11-30
CA2965495C (en) 2022-05-24
EA037255B1 (ru) 2021-02-26
KR102243243B1 (ko) 2021-04-22
CA2965495A1 (en) 2016-04-28
TWI747808B (zh) 2021-12-01
US9816110B2 (en) 2017-11-14
BR112017008022A2 (pt) 2018-02-20
MY184103A (en) 2021-03-18
US20200002732A1 (en) 2020-01-02
CN107109434A (zh) 2017-08-29
KR20170096999A (ko) 2017-08-25
AU2015335921B2 (en) 2020-06-25
EP3209785B1 (en) 2019-07-31
EP3209785A1 (en) 2017-08-30
EA201790698A1 (ru) 2018-01-31
TWI780847B (zh) 2022-10-11
US20160115502A1 (en) 2016-04-28
SG11201703149RA (en) 2018-02-27
US20240018553A1 (en) 2024-01-18
AR102420A1 (es) 2017-03-01
US11268109B2 (en) 2022-03-08
JP6668340B2 (ja) 2020-03-18
US11788102B2 (en) 2023-10-17
WO2016064999A1 (en) 2016-04-28
US12565667B2 (en) 2026-03-03
TW202144576A (zh) 2021-12-01
AU2015335921A1 (en) 2017-05-04

Similar Documents

Publication Publication Date Title
MX2017005306A (es) Novedosos sitios de integracion en celulas cho y usos de estos.
MX2020004541A (es) Edicion de genes de celulas primarias.
MX2025009055A (es) Vectores de neoantigeno de alfavirus
MX388517B (es) Polipéptidos de transposasa y sus usos.
CY1124627T1 (el) Στοχευμενη τροποποιηση γονιδιωματος αρουραιου
EA201992795A1 (ru) Способ получения эукариотических клеток с отредактированной днк и набор, используемый в этом способе
MX2025014318A (es) Vector de adenovirus de chimpance util para la administracion de neoantigenos
DK4144844T3 (da) Beskyttede dna-templates til genmodificering og øget homolog rekombination i celler og fremgangsmåder til anvendelse deraf
BR112016025519A2 (pt) engenharia genética in vivo com vetores de adenovírus
BR112018005937A2 (pt) conjugados de oligonucleotídeo de afinidade e usos destes
SA523441205B1 (ar) التحرير المتعدد للجينوم
MX2022009512A (es) Alteracion dirigida del receptor celular del complejo mayor de histocompatibilidad (mhc).
MX2017008190A (es) Metodos y composiciones para la modificacion genetica dirigida a traves de direccionamiento multiple de una sola etapa.
GB2550745A (en) Crispr hybrid DNA/RNA Polynucleotides and methods of use
CO2017011957A2 (es) Variantes de alfa-amilasa y polinucleótidos que codifican las mismas
GB201609811D0 (en) Methods, cells, systems, arrays, RNA and kits
AR103225A1 (es) Composiciones detergentes, variantes de lipasa y polinucleótidos que las codifican
EP4303232A3 (en) Chimeric antigen receptor and methods of use thereof
PE20150336A1 (es) Metodos y composiciones para la modificacion de adn objetivo dirigida por arn y para la modulacion de la transcripcion dirigida por arn
MX2016007797A (es) Metodos para integracion genomica.
PH12016500395A1 (en) Cell penetrating conjugates and methods of use thereof
MY181693A (en) Dual quencher probes
EP3237613A4 (en) Visualizing modified nucleotides and nucleic acid interactions in single cells
EP3964575A3 (en) Alpha-amylase variants and polynucleotides encoding same
EP4019625C0 (en) GENETICALLY MODIFIED MULTI-COMPONENT SYSTEM USED FOR SELECTION OF MODIFIED T-CELL RECEPTORS, T-CELLS AND ANTIGEN-PRESENTING CELLS.