MX2017005306A - Novedosos sitios de integracion en celulas cho y usos de estos. - Google Patents

Novedosos sitios de integracion en celulas cho y usos de estos.

Info

Publication number
MX2017005306A
MX2017005306A MX2017005306A MX2017005306A MX2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A
Authority
MX
Mexico
Prior art keywords
expression
integration sites
enhanced
eukaryotic
novel cho
Prior art date
Application number
MX2017005306A
Other languages
English (en)
Inventor
Chen Gang
Burakov Darya
Shen Ying
P Fandl James
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=54602002&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2017005306(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2017005306A publication Critical patent/MX2017005306A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

Se proporcionan secuencias de nucleótidos potenciadoras de la expresión para sistemas de expresión en eucariotas que permiten la expresión estable y aumentada de proteínas recombinantes en células eucariotas. Se proporcionan sitios de integración genómica que proporcionan expresión aumentada y métodos de uso de estos para la expresión de un gen de interés en una célula eucariota. Se proporcionan loci cromosómicos, secuencia, y vectores para la expresión estable y aumentada de genes en células eucariotas.
MX2017005306A 2014-10-23 2015-10-21 Novedosos sitios de integracion en celulas cho y usos de estos. MX2017005306A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462067774P 2014-10-23 2014-10-23
PCT/US2015/056653 WO2016064999A1 (en) 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof

Publications (1)

Publication Number Publication Date
MX2017005306A true MX2017005306A (es) 2018-01-09

Family

ID=54602002

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017005306A MX2017005306A (es) 2014-10-23 2015-10-21 Novedosos sitios de integracion en celulas cho y usos de estos.

Country Status (16)

Country Link
US (5) US9816110B2 (es)
EP (1) EP3209785B1 (es)
JP (1) JP6668340B2 (es)
KR (1) KR102243243B1 (es)
CN (1) CN107109434A (es)
AR (1) AR102420A1 (es)
AU (1) AU2015335921B2 (es)
BR (1) BR112017008022A2 (es)
CA (1) CA2965495C (es)
EA (1) EA037255B1 (es)
IL (1) IL251674B (es)
MX (1) MX2017005306A (es)
MY (1) MY184103A (es)
SG (1) SG11201703149RA (es)
TW (2) TWI747808B (es)
WO (1) WO2016064999A1 (es)

Families Citing this family (32)

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MX2017005306A (es) 2014-10-23 2018-01-09 Regeneron Pharma Novedosos sitios de integracion en celulas cho y usos de estos.
US11649449B2 (en) 2015-09-03 2023-05-16 CHO Plus, Inc. Hybrid cell lines for high level production of a target protein
US10329594B1 (en) 2015-09-03 2019-06-25 CHO Plus, Inc. Cell lines for high level production of protein-based pharmaceuticals
JP7096770B2 (ja) * 2016-04-20 2022-07-06 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 発現強化座位の使用に基づいた抗体を作製するための組成物および方法
BR112018071285A2 (pt) * 2016-04-20 2019-02-12 Regeneron Pharma célula, conjunto de vetores, vetor, sistema, e, método
EP3504328A1 (en) 2016-08-24 2019-07-03 Regeneron Pharmaceuticals, Inc. Host cell protein modification
EP3559236A4 (en) * 2016-12-20 2020-11-04 Development Center for Biotechnology TARGETED INTEGRATION SITES IN THE OVARIAL CELL GENOME OF THE CHINESE HAMSTER
WO2018148196A1 (en) * 2017-02-07 2018-08-16 Sigma-Aldrich Co. Llc Stable targeted integration
CN110914413A (zh) * 2017-02-17 2020-03-24 隆萨有限公司 产生腺相关病毒的哺乳动物细胞
US11959093B2 (en) 2017-03-19 2024-04-16 Applied Stemcell, Inc. Integration sites and uses thereof
US11560549B2 (en) 2017-08-11 2023-01-24 Boehringer Ingelheim International Gmbh Integration sites in CHO cells
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
CN111566208A (zh) * 2017-12-22 2020-08-21 生物辐射实验室股份有限公司 利用CRISPR/Cas基因靶向控制生物体表型
EP3728309A2 (en) * 2017-12-22 2020-10-28 Genentech, Inc. Targeted integration of nucleic acids
CN109295092B (zh) * 2018-10-30 2021-06-29 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
EP3980544A1 (en) * 2018-12-21 2022-04-13 F. Hoffmann-La Roche AG Targeted integration of nucleic acids
MX2021011903A (es) 2019-04-02 2021-10-26 Chugai Pharmaceutical Co Ltd Método para introducir un gen extraño específico del objetivo.
KR20220025806A (ko) 2019-06-26 2022-03-03 제넨테크, 인크. 핵산의 무작위 구성 표적화 통합
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
CN116096907A (zh) * 2020-06-24 2023-05-09 基因泰克公司 核酸的靶向整合
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
CA3234939A1 (en) 2021-10-18 2023-04-27 Michael Goren Eukaryotic cells comprising adenovirus-associated virus polynucleotides
IL312148A (en) 2021-10-18 2024-06-01 Regeneron Pharma Controlled transcription of polynucleotides
AR127674A1 (es) 2021-10-18 2024-02-21 Regeneron Pharma Células de mamífero que comprenden genes cas9 integrados para producir sitios de integración estables, y células de mamífero que comprenden sitios de integración estables y otros sitios
WO2023147462A2 (en) * 2022-01-28 2023-08-03 The Trustees Of Columbia University In The City Of New York Genetic modifications for xenotransplantation
CN117305334A (zh) * 2022-06-21 2023-12-29 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
US20240168018A1 (en) 2022-11-18 2024-05-23 Regeneron Pharmaceuticals, Inc. Methods for detecting and evaluating viruses and virus-like particles

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US4634665A (en) 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
ATE539161T1 (de) 1998-07-21 2012-01-15 Millipore Corp Ein ein allgegenwärtiges chromatin-öffnendes element (ucoe) enthaltendes polynucleotid
US6521419B1 (en) * 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
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MX2017005306A (es) 2014-10-23 2018-01-09 Regeneron Pharma Novedosos sitios de integracion en celulas cho y usos de estos.

Also Published As

Publication number Publication date
JP2017535258A (ja) 2017-11-30
SG11201703149RA (en) 2018-02-27
KR20170096999A (ko) 2017-08-25
JP6668340B2 (ja) 2020-03-18
CN107109434A (zh) 2017-08-29
WO2016064999A1 (en) 2016-04-28
AR102420A1 (es) 2017-03-01
TW202144576A (zh) 2021-12-01
IL251674B (en) 2020-10-29
CA2965495A1 (en) 2016-04-28
EA037255B1 (ru) 2021-02-26
US11788102B2 (en) 2023-10-17
TWI780847B (zh) 2022-10-11
AU2015335921A1 (en) 2017-05-04
EP3209785A1 (en) 2017-08-30
US20210171984A1 (en) 2021-06-10
MY184103A (en) 2021-03-18
EP3209785B1 (en) 2019-07-31
US20240018553A1 (en) 2024-01-18
US9816110B2 (en) 2017-11-14
BR112017008022A2 (pt) 2018-02-20
KR102243243B1 (ko) 2021-04-22
AU2015335921B2 (en) 2020-06-25
TW201629229A (zh) 2016-08-16
EA201790698A1 (ru) 2018-01-31
IL251674A0 (en) 2017-06-29
US11268109B2 (en) 2022-03-08
TWI747808B (zh) 2021-12-01
US20180030480A1 (en) 2018-02-01
US20200002732A1 (en) 2020-01-02
CA2965495C (en) 2022-05-24
US20160115502A1 (en) 2016-04-28

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