SG10201914098YA - Chromosomal landing pads and related uses - Google Patents

Chromosomal landing pads and related uses

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Publication number
SG10201914098YA
SG10201914098YA SG10201914098YA SG10201914098YA SG10201914098YA SG 10201914098Y A SG10201914098Y A SG 10201914098YA SG 10201914098Y A SG10201914098Y A SG 10201914098YA SG 10201914098Y A SG10201914098Y A SG 10201914098YA SG 10201914098Y A SG10201914098Y A SG 10201914098YA
Authority
SG
Singapore
Prior art keywords
chromosomal
landing pads
specific
gene
integration
Prior art date
Application number
SG10201914098YA
Inventor
Vincent Mauro
Wei Zhou
Bruce Cunningham
Gerald Edelman
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of SG10201914098YA publication Critical patent/SG10201914098YA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C12N2320/32—Special delivery means, e.g. tissue-specific
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Chromosomal Landing Pads And Related Uses Provided herein are methods for stable integration and/or expression of one or more recombinant polynucleotides in a host cell. The recombinant polynucleotides are typically integrated into the host genome at some native chromosomal integration sites. The integration can be mediated by homologous recombination or by using a hybrid recombinase targeting the specific chromosomal locations. The native chromosomal integration sites in the host cells, which support stable integration and strong transcription activities of foreign genes, are present within or adjacent to specific genes in the CHO genome, ankyrin gene (Ank2), cleavage and polyadenylation specific factor 4 gene (Cpsf4), C-Mos gene, and Nephrocystin-l/Mal gene. Also provided are methods and nucleic acid molecules for inserting site-specific recombination sequences (chromosomal landing pads) into these specific chromosomal locations. Further provided are engineered host cells which contain chromosomal landing pads or transgenes integrated into the native chromosomal integration sites. Further provided are methods and compositions (e.g., kits) for integrating and expressing heterologous polynucleotides in host cells bearing inserted chromosomal landing pads. [Figure 3]
SG10201914098YA 2011-04-05 2012-04-05 Chromosomal landing pads and related uses SG10201914098YA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201161516612P 2011-04-05 2011-04-05

Publications (1)

Publication Number Publication Date
SG10201914098YA true SG10201914098YA (en) 2020-02-27

Family

ID=46000358

Family Applications (3)

Application Number Title Priority Date Filing Date
SG10201602663RA SG10201602663RA (en) 2011-04-05 2012-04-05 Chromosomal landing pads and related uses
SG2013074778A SG194118A1 (en) 2011-04-05 2012-04-05 Chromosomal landing pads and related uses
SG10201914098YA SG10201914098YA (en) 2011-04-05 2012-04-05 Chromosomal landing pads and related uses

Family Applications Before (2)

Application Number Title Priority Date Filing Date
SG10201602663RA SG10201602663RA (en) 2011-04-05 2012-04-05 Chromosomal landing pads and related uses
SG2013074778A SG194118A1 (en) 2011-04-05 2012-04-05 Chromosomal landing pads and related uses

Country Status (9)

Country Link
US (2) US8980579B2 (en)
EP (1) EP2694661A1 (en)
JP (2) JP2014511697A (en)
KR (1) KR101999410B1 (en)
CN (2) CN103597083B (en)
AU (1) AU2012240164A1 (en)
CA (1) CA2832095A1 (en)
SG (3) SG10201602663RA (en)
WO (1) WO2012138887A1 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201602663RA (en) 2011-04-05 2016-05-30 Scripps Research Inst Chromosomal landing pads and related uses
BR112015031639A2 (en) * 2013-06-19 2019-09-03 Sigma Aldrich Co Llc target integration
AU2015335921B2 (en) 2014-10-23 2020-06-25 Regeneron Pharmaceuticals, Inc. Novel CHO integration sites and uses thereof
ES2867173T3 (en) 2015-04-13 2021-10-20 Harvard College Production and monitoring of metabolites in cells
CL2016001661A1 (en) 2016-06-29 2017-03-03 Univ Chile Hybrid promoter of ß-actin (from cricetulus griseus) and cytomegalovirus (cmv), with a region rich in dinucleotides, cytosine-guanine, vectors, cell lines, procedure to produce recombinant proteins.
US20210309988A1 (en) * 2017-02-07 2021-10-07 Sigma-Aldrich Co. Llc Stable targeted integration
US20200002727A1 (en) * 2017-02-17 2020-01-02 Lonza Ltd. Multi-site specific integration cells for difficult to express proteins
GB201703418D0 (en) 2017-03-03 2017-04-19 Ge Healthcare Bio Sciences Ab Method for cell line development
CN111212913A (en) * 2017-05-16 2020-05-29 凯恩生物科学股份有限公司 Multiplex assay
GB201712678D0 (en) * 2017-08-07 2017-09-20 Glaxosmithkline Biologicals Sa Process for the manipulation of nucleic acids
WO2020041149A2 (en) * 2018-08-18 2020-02-27 Cairn Biosciences, Inc. Stem cell derived lineage barcoding
AU2020252840A1 (en) * 2019-04-02 2021-11-04 Chugai Seiyaku Kabushiki Kaisha Method of introducing target-specific foreign gene
EP3956460A2 (en) * 2019-04-18 2022-02-23 Sigma-Aldrich Co. LLC Stable targeted integration
CA3157472A1 (en) * 2019-10-09 2021-04-15 The Jackson Laboratory High frequency targeted animal transgenesis
GB202019484D0 (en) * 2020-12-10 2021-01-27 Univ Edinburgh CHO cell modification
WO2023064871A1 (en) 2021-10-14 2023-04-20 Asimov Inc. Integrases, landing pad architectures, and engineered cells comprising the same
CA3237804A1 (en) 2021-11-09 2023-05-19 Asimov Inc. Stable production systems for aav vector production
CN113999873B (en) * 2021-12-31 2022-05-20 北京市疾病预防控制中心 Construction method and application of genetically modified non-human animal

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5175385A (en) 1987-09-03 1992-12-29 Ohio University/Edison Animal Biotechnolgy Center Virus-resistant transgenic mice
US5175384A (en) 1988-12-05 1992-12-29 Genpharm International Transgenic mice depleted in mature t-cells and methods for making transgenic mice
WO2008127680A2 (en) * 2007-04-11 2008-10-23 The Jackson Laboratory Diagnosis and treatment of diseases caused by misfolded proteins
SG10201602663RA (en) 2011-04-05 2016-05-30 Scripps Research Inst Chromosomal landing pads and related uses

Also Published As

Publication number Publication date
JP2014511697A (en) 2014-05-19
AU2012240164A1 (en) 2013-11-14
CN103597083A (en) 2014-02-19
JP2018011602A (en) 2018-01-25
EP2694661A1 (en) 2014-02-12
CN103597083B (en) 2016-05-18
US20120258541A1 (en) 2012-10-11
US10017786B2 (en) 2018-07-10
CA2832095A1 (en) 2012-10-11
WO2012138887A1 (en) 2012-10-11
CN105969807A (en) 2016-09-28
JP6441425B2 (en) 2018-12-19
KR20140019430A (en) 2014-02-14
US8980579B2 (en) 2015-03-17
KR101999410B1 (en) 2019-07-11
SG10201602663RA (en) 2016-05-30
SG194118A1 (en) 2013-11-29
US20150152437A1 (en) 2015-06-04

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