AR102420A1 - Sitios de integración en celulas cho y sus usos - Google Patents

Sitios de integración en celulas cho y sus usos

Info

Publication number
AR102420A1
AR102420A1 ARP150103445A ARP150103445A AR102420A1 AR 102420 A1 AR102420 A1 AR 102420A1 AR P150103445 A ARP150103445 A AR P150103445A AR P150103445 A ARP150103445 A AR P150103445A AR 102420 A1 AR102420 A1 AR 102420A1
Authority
AR
Argentina
Prior art keywords
expression
integration sites
cho cells
eukaryotic
improved
Prior art date
Application number
ARP150103445A
Other languages
English (en)
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=54602002&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR102420(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of AR102420A1 publication Critical patent/AR102420A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells

Abstract

Se proporcionan secuencias nucleotídicas de aumento de la expresión para sistemas de expresión eucarióticos que permiten la expresión mejorada y estable de proteínas recombinantes en células eucarióticas. Se proporcionan sitios de integración genómica que proporcionan expresión mejorada y métodos de uso de los mismos para la expresión de un gen de interés en una célula eucariótica. Se proporcionan sitios cromosómicos, secuencias, y vectores para la expresión mejorada y estable de genes en células eucarióticas.
ARP150103445A 2014-10-23 2015-10-23 Sitios de integración en celulas cho y sus usos AR102420A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201462067774P 2014-10-23 2014-10-23

Publications (1)

Publication Number Publication Date
AR102420A1 true AR102420A1 (es) 2017-03-01

Family

ID=54602002

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP150103445A AR102420A1 (es) 2014-10-23 2015-10-23 Sitios de integración en celulas cho y sus usos

Country Status (16)

Country Link
US (5) US9816110B2 (es)
EP (1) EP3209785B1 (es)
JP (1) JP6668340B2 (es)
KR (1) KR102243243B1 (es)
CN (1) CN107109434A (es)
AR (1) AR102420A1 (es)
AU (1) AU2015335921B2 (es)
BR (1) BR112017008022A2 (es)
CA (1) CA2965495C (es)
EA (1) EA037255B1 (es)
IL (1) IL251674B (es)
MX (1) MX2017005306A (es)
MY (1) MY184103A (es)
SG (1) SG11201703149RA (es)
TW (2) TWI780847B (es)
WO (1) WO2016064999A1 (es)

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WO2018175153A1 (en) * 2017-03-19 2018-09-27 Applied Stemcell, Inc. Novel integration sites and uses thereof
CN110997925A (zh) * 2017-08-11 2020-04-10 勃林格殷格翰国际有限公司 Cho细胞中的整合位点
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
WO2019126562A1 (en) * 2017-12-22 2019-06-27 Bio-Rad Laboratories, Inc. Controlling phenotype of organisms with crispr/cas gene targeting
BR112020012591A2 (pt) * 2017-12-22 2020-11-24 Genentech, Inc. células hospedeiras de integração direcionada (ti), células hospedeiras ti, métodos para preparar uma célula hospedeira ti, métodos para expressar um polipeptídeo de interesse e vetores
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CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109295092B (zh) * 2018-10-30 2021-06-29 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
SG11202106523SA (en) * 2018-12-21 2021-07-29 Genentech Inc Targeted integration of nucleic acids
JPWO2020204055A1 (es) 2019-04-02 2020-10-08
KR20220025806A (ko) 2019-06-26 2022-03-03 제넨테크, 인크. 핵산의 무작위 구성 표적화 통합
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
KR20230027043A (ko) * 2020-06-24 2023-02-27 제넨테크, 인크. 핵산의 표적화 통합
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
WO2023069931A1 (en) 2021-10-18 2023-04-27 Regeneron Pharmaceuticals, Inc. Mammalian cells comprising integrated cas9 genes to produce stable integration sites, and mammalian cells comprising stable integration sites and other sites
AU2022371200A1 (en) 2021-10-18 2024-05-02 Regeneron Pharmaceuticals, Inc. Controlled transcription of polynucleotides
TW202323529A (zh) 2021-10-18 2023-06-16 美商再生元醫藥公司 包括腺病毒相關病毒聚核苷酸的真核細胞
WO2023147462A2 (en) * 2022-01-28 2023-08-03 The Trustees Of Columbia University In The City Of New York Genetic modifications for xenotransplantation
CN117305334A (zh) * 2022-06-21 2023-12-29 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法

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Also Published As

Publication number Publication date
MY184103A (en) 2021-03-18
TW201629229A (zh) 2016-08-16
US20210171984A1 (en) 2021-06-10
US20200002732A1 (en) 2020-01-02
JP6668340B2 (ja) 2020-03-18
AU2015335921A1 (en) 2017-05-04
CA2965495C (en) 2022-05-24
AU2015335921B2 (en) 2020-06-25
EA037255B1 (ru) 2021-02-26
TWI747808B (zh) 2021-12-01
US9816110B2 (en) 2017-11-14
SG11201703149RA (en) 2018-02-27
CA2965495A1 (en) 2016-04-28
IL251674A0 (en) 2017-06-29
IL251674B (en) 2020-10-29
JP2017535258A (ja) 2017-11-30
MX2017005306A (es) 2018-01-09
TW202144576A (zh) 2021-12-01
US20180030480A1 (en) 2018-02-01
EA201790698A1 (ru) 2018-01-31
KR102243243B1 (ko) 2021-04-22
US11268109B2 (en) 2022-03-08
TWI780847B (zh) 2022-10-11
US11788102B2 (en) 2023-10-17
US20160115502A1 (en) 2016-04-28
EP3209785B1 (en) 2019-07-31
WO2016064999A1 (en) 2016-04-28
US20240018553A1 (en) 2024-01-18
KR20170096999A (ko) 2017-08-25
CN107109434A (zh) 2017-08-29
BR112017008022A2 (pt) 2018-02-20
EP3209785A1 (en) 2017-08-30

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