EA201691297A1 - Среда для культивирования клеток - Google Patents

Среда для культивирования клеток

Info

Publication number
EA201691297A1
EA201691297A1 EA201691297A EA201691297A EA201691297A1 EA 201691297 A1 EA201691297 A1 EA 201691297A1 EA 201691297 A EA201691297 A EA 201691297A EA 201691297 A EA201691297 A EA 201691297A EA 201691297 A1 EA201691297 A1 EA 201691297A1
Authority
EA
Eurasian Patent Office
Prior art keywords
medium
serum substitute
cultivating cells
phosphatidylcholine
assumed
Prior art date
Application number
EA201691297A
Other languages
English (en)
Inventor
Адам Элофи
Аллан Вебер
Original Assignee
ЭССЕНШЛ ФАРМАСЬЮТИКАЛЗ, ЭлЭлСи
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ЭССЕНШЛ ФАРМАСЬЮТИКАЛЗ, ЭлЭлСи filed Critical ЭССЕНШЛ ФАРМАСЬЮТИКАЛЗ, ЭлЭлСи
Publication of EA201691297A1 publication Critical patent/EA201691297A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/35—Polyols, e.g. glycerin, inositol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C12N2500/62—DMSO
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/32—Polylysine, polyornithine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Biophysics (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Настоящая заявка относится в целом к среде, например заменителю сыворотки, добавке к среде, полной среде или среде для криоконсервации, содержащей основную физиологическую буферную жидкую смесь и липосомы, содержащие холестерин, фосфатидилхолин и жирные кислоты. Предполагается, что среда обеспечивает преимущества, улучшающие рост клеток при культивировании, по сравнению с клетками, культивируемыми без применения заменителя сыворотки, описанного в настоящей заявке.
EA201691297A 2013-12-20 2014-12-19 Среда для культивирования клеток EA201691297A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361918833P 2013-12-20 2013-12-20
PCT/US2014/071388 WO2015095651A1 (en) 2013-12-20 2014-12-19 Media for cell culture

Publications (1)

Publication Number Publication Date
EA201691297A1 true EA201691297A1 (ru) 2016-11-30

Family

ID=53399356

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201691297A EA201691297A1 (ru) 2013-12-20 2014-12-19 Среда для культивирования клеток

Country Status (16)

Country Link
US (2) US9637721B2 (ru)
EP (1) EP3083978A4 (ru)
JP (1) JP2017500893A (ru)
KR (1) KR20160125352A (ru)
CN (1) CN106062205A (ru)
AR (1) AR098875A1 (ru)
AU (1) AU2014364368A1 (ru)
BR (1) BR112016014488A2 (ru)
CA (1) CA2934487A1 (ru)
CL (1) CL2016001553A1 (ru)
EA (1) EA201691297A1 (ru)
IL (1) IL246262A0 (ru)
MX (1) MX2016008251A (ru)
SG (1) SG11201604994UA (ru)
TW (1) TW201527534A (ru)
WO (1) WO2015095651A1 (ru)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10918102B2 (en) 2014-03-13 2021-02-16 The General Hospital Corporation Devices and methods to improve and assess viability of human livers
IL305070A (en) 2014-12-30 2023-10-01 Cell Cure Neurosciences Ltd RPE cell populations and methods for their production
KR20180068997A (ko) 2015-10-15 2018-06-22 웨이크 포리스트 유니버시티 헬스 사이언시즈 인 비트로 간 구조체의 제조방법 및 그의 용도
KR20180068994A (ko) 2015-10-16 2018-06-22 웨이크 포리스트 유니버시티 헬스 사이언시즈 다층 기도 오가노이드 및 그의 제조 및 사용 방법
WO2017096139A1 (en) * 2015-12-03 2017-06-08 Boston Medical Center Corporation B cell-based cancer immunotherapy
CN105567628B (zh) * 2016-01-30 2019-07-12 令世鑫 一种全悬浮培养mdck细胞的低血清培养基
CN105695407B (zh) * 2016-03-15 2019-04-16 佰通生物技术(苏州)有限公司 一种对干细胞具有活化作用的微量元素组合物及其应用
WO2017156762A1 (en) 2016-03-18 2017-09-21 Institute Of Biophysics, Chinese Academy Of Sciences A cell culture medium and culture medium supplement
TWI734775B (zh) * 2016-04-26 2021-08-01 美商美國泰福生技股份有限公司 細胞培養基
CN106119186B (zh) * 2016-06-24 2019-10-08 肇庆大华农生物药品有限公司 一种用于全悬浮培养mdck细胞的无血清培养基及其制备方法
CN106497872B (zh) * 2016-12-01 2019-10-11 北京欣博睿丰医疗科技发展有限公司 骨骼肌干细胞无血清培养基及其制备方法和应用
WO2018144693A1 (en) * 2017-02-01 2018-08-09 Orig3N, Inc. Systems and methods for customizing cell culture media for optimized cell proliferation based on genetic traits of cells
CA3058306A1 (en) * 2017-03-28 2018-10-04 Ajinomoto Co., Inc. Additive for undifferentiation maintaining medium
CN107177542A (zh) * 2017-05-25 2017-09-19 句容亿格纳米材料厂 一种促进培养基中谷氨酰胺溶解的优化方法
JP6913694B2 (ja) * 2017-05-31 2021-08-04 プロモセル・ゲー・エム・ベー・ハー 多能性幹細胞のための培養培地
US12058996B2 (en) * 2017-06-14 2024-08-13 The General Hospital Corporation High subzero cryopreservation
CN107488625B (zh) * 2017-08-31 2018-12-21 广州蕊特生物科技有限公司 一种胎牛血清替代物及其制备方法和应用
CN109395095B (zh) * 2017-09-18 2021-09-24 武汉原生原代生物医药科技有限公司 体内用生物膜及其制备方法和用途
CN111278441A (zh) * 2017-10-11 2020-06-12 巴斯特生物技术 用于治疗纤维化的组合物和方法
AU2018348712B2 (en) * 2017-10-13 2024-05-30 Boehringer Ingelheim International Gmbh Perfusion medium
WO2019081609A1 (en) * 2017-10-24 2019-05-02 Fundación Instituto De Investigación Sanitaria De Santiago De Compostela (Fidis) NANOSYSTEMS FOR CELL PROLIFERATION
KR102569522B1 (ko) * 2017-11-24 2023-08-24 주식회사 차바이오랩 세포 동결 보존용 조성물 및 이를 이용한 세포 동결 보존 방법
SG11202005795TA (en) * 2017-12-29 2020-07-29 Cell Cure Neurosciences Ltd Retinal pigment epithelium cell compositions
CN108853489B (zh) * 2018-06-29 2019-07-02 陕西诺威利华生物科技有限公司 一种利用无血清培养基生产pedv弱毒疫苗的方法
CN108815516B (zh) * 2018-06-29 2019-03-15 陕西诺威利华生物科技有限公司 一种利用无血清培养基生产pedv灭活疫苗的方法
CN109234223B (zh) * 2018-11-21 2021-01-19 南京基蛋生物医药有限公司 低蛋白无血清细胞培养基
KR102271949B1 (ko) * 2019-04-15 2021-07-02 순천향대학교 산학협력단 포스파티딜콜린을 함유하는 선충 배양용 조성물 및 이를 이용한 선충의 배양방법
WO2020218584A1 (ja) * 2019-04-26 2020-10-29 味の素株式会社 細胞培養用組成物
KR102132457B1 (ko) * 2019-05-10 2020-07-09 주식회사 바이오솔루션 세포외 소포를 고농도로 배양하기 위한 세포 배양배지 및 상기 세포 배양배지를 이용한 세포외 소포 고 함유 조건화 배양액의 제조방법
CN109943526B (zh) * 2019-05-23 2019-09-13 广州赛莱拉干细胞科技股份有限公司 一种促间充质干细胞增殖的无血清多肽组合物
WO2021078935A1 (en) * 2019-10-25 2021-04-29 Fundació Centre De Regulació Genòmica Culture media for mycoplasma
EP4064837A4 (en) * 2019-11-29 2023-01-04 Rutgers, the State University of New Jersey COMPOSITIONS, KITS AND METHODS FOR STORING BLOOD PRODUCTS AND METHODS OF USE THEREOF
US20230070582A1 (en) * 2020-01-21 2023-03-09 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Utilization of fgf activators in culture media
CN111849860B (zh) * 2020-06-01 2021-12-21 浙江大学 一种利用铁元素调控肠道干细胞分化的方法及应用
CN111534478A (zh) * 2020-06-24 2020-08-14 肇庆大华农生物药品有限公司 用于培养mdck细胞系的培养基及其制备方法
CN112514892B (zh) * 2020-12-25 2022-06-14 广州赛莱拉干细胞科技股份有限公司 一种外泌体冻存保护液及其制备方法
CN112501113B (zh) * 2021-02-03 2021-04-27 上海奥浦迈生物科技股份有限公司 一种hek293细胞组合梯度培养基及其用途
CN113061584A (zh) * 2021-05-14 2021-07-02 赵峻岭 一种能促进腺病毒生长的培养基添加物
US11878036B2 (en) 2022-05-25 2024-01-23 Neuvian LLC Vaginal care compositions and methods of improving vaginal health
WO2024024892A1 (ja) * 2022-07-28 2024-02-01 公立大学法人山陽小野田市立山口東京理科大学 凍結保存用の組成物、凍結保存方法、及び凍結した細胞又は生体組織

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4721676A (en) * 1982-12-14 1988-01-26 Genencor, Inc. Novel thermophile isolate having thermostable hydrolytic activity
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
DE3827578A1 (de) 1988-08-13 1990-02-15 Siepmann Friedrich W Verfahren und vorrichtung zur bestimmung oxidierbarer wasserinhaltsstoffe einer waessrigen probenfluessigkeit
US6048728A (en) 1988-09-23 2000-04-11 Chiron Corporation Cell culture medium for enhanced cell growth, culture longevity, and product expression
US5834312A (en) 1990-01-29 1998-11-10 Hy-Gene, Inc. Process and media for the growth of human epithelia
US5952198A (en) * 1995-05-04 1999-09-14 Bayer Corporation Production of recombinant Factor VIII in the presence of liposome-like substances of mixed composition
WO1997006784A1 (en) 1995-08-15 1997-02-27 Universite Libre De Bruxelles Liposomes preparation method and plant
US6140121A (en) * 1995-10-19 2000-10-31 Advanced Reproduction Technologies, Inc. Methods and compositions to improve germ cell and embryo survival and function
JP2000517188A (ja) 1996-08-30 2000-12-26 ライフ テクノロジーズ，インコーポレイテッド 無血清哺乳動物細胞培養培地およびその使用
DE69738806D1 (de) 1996-10-10 2008-08-14 Invitrogen Corp Tierzellkulturmedium mit pflanzlichen nährstoffen
US6833271B2 (en) * 1996-12-04 2004-12-21 Medi-Cult A/S Serum-free cell culture media
US5985538A (en) 1997-08-01 1999-11-16 Saint Barnabas Medical Center Cryopreservation and cell culture medium comprising less than 50 mM sodium ions and greater than 100 mM choline salt
GB9817805D0 (en) 1998-08-14 1998-10-14 Danisco Use of a composition
DK1226233T3 (da) 1999-08-05 2011-10-03 Abt Holding Co Multipotente voksne stamceller og fremgangsmåder til isolering heraf
US7112576B1 (en) * 1999-12-10 2006-09-26 Regents Of The University Of Minnesota Compositions and methods for cryopreservation of peripheral blood lymphocytes
US7455983B2 (en) 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
WO2003064598A2 (en) 2002-01-25 2003-08-07 Genzyme Corporation Serum-free media for chondrocytes and methods of use thereof
EP1475434A1 (en) * 2003-05-09 2004-11-10 Oncoscience AG Method for storing tumor cells
EP1711053A2 (en) 2004-02-02 2006-10-18 I.M.T. Interface Multigrad Technology Ltd. Biological material and methods and solutions for preservation thereof
US8252591B2 (en) 2004-05-07 2012-08-28 Whitehead Institute For Biomedical Research Hormone responsive tissue culture system and uses thereof
US20050287666A1 (en) 2004-06-29 2005-12-29 Invitrogen Corporation Cell culture medium comprising transition metals or trace elements
EP1809739B1 (en) 2004-07-13 2014-10-15 Asterias Biotherapeutics, Inc. Medium for growing human embryonic stem cells
WO2006045064A2 (en) 2004-10-20 2006-04-27 Whitehead Institute For Biomedical Research Cultured hematopoietic stem cells and method for expansion and analysis thereof
RS51913B (en) 2004-11-02 2012-02-29 Ares Trading S.A. Mammalian cell culture medium without serum content
CA2595638A1 (en) * 2005-01-28 2006-08-03 Canadian Blood Services A method of cryopreserving cells and tissues by liposomal delivery of sugars to enhance post-thaw viability
WO2007016366A2 (en) 2005-07-29 2007-02-08 Yale University Defined culture conditions of human embryonic stem cells
AU2006328944B2 (en) 2005-12-21 2011-08-25 Sentoclone International Ab Method for treating disseminated cancer
EP1994141B1 (en) 2006-02-23 2017-11-15 ViaCyte, Inc. Compositions and methods useful for culturing differentiable cells
JP5080779B2 (ja) * 2006-10-25 2012-11-21 テルモ株式会社 リポソーム製剤の製造方法
JP2010525836A (ja) 2007-05-04 2010-07-29 ホワイトヘッド・インスティテュート・フォー・バイオメディカル・リサーチ ヒト造血幹細胞のｅｘｖｉｖｏ増殖
US20110244502A1 (en) 2007-08-10 2011-10-06 Whitehead Institute For Biomedical Research Hormone responsive tissue culture system and uses thereof
FI20096288A0 (fi) * 2009-12-04 2009-12-04 Kristiina Rajala Formulations and methods for culturing stem cells
KR20140099269A (ko) * 2011-11-11 2014-08-11 이센셜 파마수티컬스 엘엘씨 혈청 대체물질 및 불안정한 인자를 포함하는 키트

Also Published As

Publication number Publication date
IL246262A0 (en) 2016-07-31
US9637721B2 (en) 2017-05-02
CA2934487A1 (en) 2015-06-25
AR098875A1 (es) 2016-06-22
EP3083978A4 (en) 2017-08-30
US20170198251A1 (en) 2017-07-13
TW201527534A (zh) 2015-07-16
JP2017500893A (ja) 2017-01-12
KR20160125352A (ko) 2016-10-31
AU2014364368A1 (en) 2016-07-07
EP3083978A1 (en) 2016-10-26
CN106062205A (zh) 2016-10-26
MX2016008251A (es) 2016-10-13
WO2015095651A1 (en) 2015-06-25
US20150175956A1 (en) 2015-06-25
BR112016014488A2 (pt) 2017-08-08
SG11201604994UA (en) 2016-07-28
CL2016001553A1 (es) 2017-03-17

Similar Documents

Publication Publication Date Title
EA201691297A1 (ru) Среда для культивирования клеток
EA201690600A1 (ru) Метаболически оптимизированная клеточная культура
BR112014031658A2 (pt) método mixotrófico de aquicultura
MX352823B (es) Formulaciones de proteinas que contienen aminoacidos.
PE20140770A1 (es) Composiciones de lipidos algales y metodos para prepararlas y utilizarlas
BR112014003708A2 (pt) microorganismos métodos para a produção de 2,4-pentadienoato, butadieno, propileno, 1,3-butanodiol e álcoois relacionados
BR112015006546B8 (pt) Entidade descontínua tridimensional, métodos para fabricar uma entidade descontõnua tridimensional, para fabricar uma matriz de cultura celular, para transportar c lulas, e para cultura tridimensional ou bidimensional de c lulas ou tecidos, matriz de cultura celular, artigo para cultura celular, uso de uma entidade descontõnua tridimensional ou de um artigo, e, kit
EA201591817A1 (ru) Не содержащая сыворотки среда для культивирования клеток
EA202091407A1 (ru) Состав бессывороточной среды для культивирования клеток и способы его применения
EA201490945A1 (ru) Набор, содержащий заменитель сыворотки и лабильные факторы
MX2015007361A (es) Produccion mediada por la proteina portadora de acilo de los derivados de acidos grasos.
MX2016005824A (es) Metodo de cultivo celular.
EA201590550A1 (ru) Композиции и способы получения гликопротеинов
EA201291293A1 (ru) Сельскохозяйственные препараты с ацилморфолинами и полярными апротонными сорастворителями
AR092630A1 (es) Metodos y composiciones para prevenir la incorporacion erronea de norleucina en proteinas
EA201491785A1 (ru) Способ ферментации
RU2012144595A (ru) Способ получения культуры симбиотических бактерий энтомопатогенных нематод
IN2014CN01889A (ru)
BR112017009306A2 (pt) método de cultivo de algas
EA201591558A1 (ru) Составы и способы увеличения производства рекомбинантного белка
UA81055U (ru) Способ культивирования каланоидных копепод arctodiaptomus salinus (daday)
TR201902764T4 (tr) Besin bileşimi, söz konusu besin bileşimini içeren gıda takviyesi ve söz konusu gıda takviyesini içeren kit.
RU2011152718A (ru) Способ получения жидкого мицелия для использования в обогащении белком кормов для животных
MD191Z5 (ru) Питательная среда для культивирования клеток животного происхождения
PL408906A1 (pl) Pożywka do namnażania lawendy wąskolistnej odmiany Munstead