EA201391666A1 - Рекомбинантная экспрессия растворимого интерферона - Google Patents

Рекомбинантная экспрессия растворимого интерферона

Info

Publication number
EA201391666A1
EA201391666A1 EA201391666A EA201391666A EA201391666A1 EA 201391666 A1 EA201391666 A1 EA 201391666A1 EA 201391666 A EA201391666 A EA 201391666A EA 201391666 A EA201391666 A EA 201391666A EA 201391666 A1 EA201391666 A1 EA 201391666A1
Authority
EA
Eurasian Patent Office
Prior art keywords
recombinant expression
soluble interferon
gene
interferon
recombinant
Prior art date
Application number
EA201391666A
Other languages
English (en)
Other versions
EA027218B1 (ru
Inventor
Ральф Шиллинг
Беттина Дидерих
Original Assignee
Рихтер-Хельм Биотек Гмбх Унд Ко. Кг
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Рихтер-Хельм Биотек Гмбх Унд Ко. Кг filed Critical Рихтер-Хельм Биотек Гмбх Унд Ко. Кг
Publication of EA201391666A1 publication Critical patent/EA201391666A1/ru
Publication of EA027218B1 publication Critical patent/EA027218B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Настоящее изобретение относится в общем к области рекомбинантной экспрессии генов в клетках-хозяевах. В частности, изобретение относится к клетке-хозяину для рекомбинантной экспрессии белка интерферона млекопитающего, причем упомянутая клетка включает: (а) функциональный эндогенный trxBген и (b) инактивированный эндогенный gorген. Изобретение, кроме того, относится к использованию таких клеток-хозяев для рекомбинантной экспрессии белка интерферона. Изобретение также предлагает способ рекомбинантной экспрессии растворимого интерферона в клетке-хозяине.
EA201391666A 2011-05-26 2012-05-21 Рекомбинантная экспрессия растворимого интерферона EA027218B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11167761A EP2527424A1 (en) 2011-05-26 2011-05-26 Recombinant expression of soluble Interferon
PCT/EP2012/059373 WO2012160027A1 (en) 2011-05-26 2012-05-21 Recombinant expression of soluble interferon

Publications (2)

Publication Number Publication Date
EA201391666A1 true EA201391666A1 (ru) 2014-03-31
EA027218B1 EA027218B1 (ru) 2017-07-31

Family

ID=46149442

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201391666A EA027218B1 (ru) 2011-05-26 2012-05-21 Рекомбинантная экспрессия растворимого интерферона

Country Status (9)

Country Link
EP (2) EP2527424A1 (ru)
AR (1) AR086556A1 (ru)
DK (1) DK2714890T3 (ru)
EA (1) EA027218B1 (ru)
GE (1) GEP201606542B (ru)
HU (1) HUE026998T2 (ru)
MD (1) MD4384C1 (ru)
UA (1) UA110831C2 (ru)
WO (1) WO2012160027A1 (ru)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2796556A1 (en) 2013-04-25 2014-10-29 Rijksuniversiteit Groningen Improved means and methods for expressing recombinant proteins
CN106399321A (zh) * 2016-08-27 2017-02-15 华南农业大学 一种制备融合表达重组鸡α干扰素的生产工艺
CN111647614B (zh) * 2019-12-31 2022-07-12 北京宝易生物技术有限公司 一种产犬α-干扰素的重组菌的构建方法及其应用
CN111172184A (zh) * 2020-03-10 2020-05-19 大连理工大学 一种基于基因修饰得到人重组干扰素的融合基因、融合蛋白及其制备方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5510472A (en) * 1979-11-21 1996-04-23 Yeda Research And Development Co. Ltd. Production of recombinant human interferon-beta2
US5554513A (en) * 1979-11-21 1996-09-10 Yeda Research & Development Co. Ltd. Production of recombinant human interferon-beta2
MX9203641A (es) 1983-12-16 1992-07-01 Genentech Inc Interferones gamma recombinantes que poseen estabilidad mejorada y metodos biotecnologicos para su obtencion.
DE3730331A1 (de) 1987-09-10 1989-03-30 Basf Ag Neue polypeptide, verfahren zur herstellung, die vektoren dafuer und arzneimittel daraus
US5676942A (en) 1992-02-10 1997-10-14 Interferon Sciences, Inc. Composition containing human alpha interferon species proteins and method for use thereof
JP3467515B2 (ja) 1994-11-01 2003-11-17 財団法人ルイ・パストゥール医学研究センター インターフェロン活性の測定法
US5770191A (en) 1995-05-24 1998-06-23 University Of Florida Active C-terminal peptides of interferon--gamma and their use
DE19535853C2 (de) 1995-09-18 1999-04-01 Fraunhofer Ges Forschung Varianten des rekombinanten humanen Interferon-gamma, Verfahren zu ihrer Herstellung und ihre Verwendung
JP3757281B2 (ja) * 2003-03-25 2006-03-22 独立行政法人酒類総合研究所 ポリガラクツロナーゼの大腸菌による生産システム
EP2634252B1 (en) * 2005-02-11 2018-12-19 University of Southern California Method of expressing proteins with disulfide bridges
CN101225398A (zh) * 2007-01-18 2008-07-23 王尚武 由原核细胞制备活性t-PA的技术
EP2000480A1 (en) * 2007-06-07 2008-12-10 Institut Pasteur Method for the production of high-level soluble human recombinant interferon alpha in e. coli and vectors useful for such a production
US20090258394A1 (en) 2008-04-10 2009-10-15 Institut Pasteur Method for the production of high-level soluble human recombinant interferon alpha in e. coli and vectors useful for such a production
CN101921330B (zh) * 2010-06-02 2013-07-17 北京三元基因工程有限公司 重组人干扰素α1b突变体及其制备方法
CN102978276B (zh) * 2012-12-24 2015-11-04 丁兆明 一种快速检测细胞中谷胱甘肽的方法

Also Published As

Publication number Publication date
EP2714890B1 (en) 2015-09-16
AR086556A1 (es) 2014-01-08
EP2527424A1 (en) 2012-11-28
MD4384C1 (ru) 2016-06-30
GEP201606542B (en) 2016-09-26
MD20130099A2 (ru) 2014-06-30
DK2714890T3 (en) 2015-11-30
EP2714890A1 (en) 2014-04-09
HUE026998T2 (en) 2016-08-29
EA027218B1 (ru) 2017-07-31
UA110831C2 (uk) 2016-02-25
WO2012160027A1 (en) 2012-11-29
MD4384B1 (ru) 2015-11-30

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Legal Events

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QB4A Registration of a licence in a contracting state
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG TJ TM