MX344970B - Medio de cultivo celular para la expresion de la proteina adamts. - Google Patents

Medio de cultivo celular para la expresion de la proteina adamts.

Info

Publication number
MX344970B
MX344970B MX2013009470A MX2013009470A MX344970B MX 344970 B MX344970 B MX 344970B MX 2013009470 A MX2013009470 A MX 2013009470A MX 2013009470 A MX2013009470 A MX 2013009470A MX 344970 B MX344970 B MX 344970B
Authority
MX
Mexico
Prior art keywords
culture medium
cell culture
protein expression
adamts protein
adamts
Prior art date
Application number
MX2013009470A
Other languages
English (en)
Inventor
Grillberger Leopold
Spenger Alexandra
Hasslacher Meinhard
Grillberger Rana
Reiter Manfred
Original Assignee
Baxalta Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxalta Inc filed Critical Baxalta Inc
Publication of MX344970B publication Critical patent/MX344970B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Reproductive Health (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)

Abstract

La presente invención se refiere a un método para producir una composición ADAMTS, el método comprende los pasos de: (a) cultivar una célula que contiene un ácido nucleico que codifica una proteína ADAMTS en un medio de cultivo libre de proteína animal; (b) eliminar una fracción del sobrenadante del cultivo; (c) realizar una etapa de filtración o centrifugación para eliminar cualquier célula residual; (d) realizar una etapa de ultrafiltración para concentrar la proteína ADAMTS; y ( e) realizar una etapa de diafiltración un amortiguador que comprende al menos aproximadamente 0.5 µM de zinc y al menos aproximadamente 0.1 mM de calcio; preparando de ese modo una composición ADAMTS.
MX2013009470A 2009-07-31 2012-01-27 Medio de cultivo celular para la expresion de la proteina adamts. MX344970B (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US23047709P 2009-07-31 2009-07-31

Publications (1)

Publication Number Publication Date
MX344970B true MX344970B (es) 2017-01-12

Family

ID=42668009

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2012001261A MX2012001261A (es) 2009-07-31 2010-07-30 Medio de cultivo de celula para la expresion de proteina adamts.
MX2013009470A MX344970B (es) 2009-07-31 2012-01-27 Medio de cultivo celular para la expresion de la proteina adamts.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2012001261A MX2012001261A (es) 2009-07-31 2010-07-30 Medio de cultivo de celula para la expresion de proteina adamts.

Country Status (22)

Country Link
US (7) US8313926B2 (es)
EP (1) EP2459702B1 (es)
JP (5) JP5451884B2 (es)
KR (5) KR101574056B1 (es)
CN (2) CN102549145B (es)
AU (1) AU2010278751B2 (es)
BR (2) BR112012002151B1 (es)
CA (1) CA2769361A1 (es)
CO (1) CO6501191A2 (es)
EA (1) EA023193B1 (es)
ES (1) ES2583258T3 (es)
HK (1) HK1171790A1 (es)
HR (1) HRP20160783T1 (es)
HU (1) HUE029110T2 (es)
IN (1) IN2012DN00801A (es)
MX (2) MX2012001261A (es)
NZ (1) NZ597727A (es)
PL (1) PL2459702T3 (es)
PT (1) PT2459702T (es)
SG (1) SG178194A1 (es)
SI (1) SI2459702T1 (es)
WO (1) WO2011014838A1 (es)

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Publication number Publication date
CN104962539B (zh) 2019-09-17
US20110086413A1 (en) 2011-04-14
EA023193B1 (ru) 2016-05-31
AU2010278751B2 (en) 2014-05-15
BR122021005965B1 (pt) 2022-01-25
KR101808496B1 (ko) 2017-12-12
CN102549145B (zh) 2015-05-20
US20150329848A1 (en) 2015-11-19
KR101573972B1 (ko) 2015-12-02
US11254921B2 (en) 2022-02-22
KR101574056B1 (ko) 2015-12-02
PT2459702T (pt) 2016-08-02
JP2019201661A (ja) 2019-11-28
JP6511007B2 (ja) 2019-05-08
MX2012001261A (es) 2012-05-22
US8759026B2 (en) 2014-06-24
EP2459702A1 (en) 2012-06-06
ES2583258T3 (es) 2016-09-20
JP7083059B2 (ja) 2022-06-09
HK1171790A1 (zh) 2013-04-05
CO6501191A2 (es) 2012-08-15
CN104962539A (zh) 2015-10-07
HRP20160783T1 (hr) 2016-08-12
US8313926B2 (en) 2012-11-20
EA201270224A1 (ru) 2012-09-28
KR101912552B1 (ko) 2018-10-26
BR112012002151B1 (pt) 2021-07-06
AU2010278751A1 (en) 2012-02-09
CN102549145A (zh) 2012-07-04
US20130071906A1 (en) 2013-03-21
HUE029110T2 (en) 2017-02-28
SI2459702T1 (sl) 2016-11-30
US10724024B2 (en) 2020-07-28
US20200308566A1 (en) 2020-10-01
JP2018093881A (ja) 2018-06-21
KR20170140411A (ko) 2017-12-20
JP2021112210A (ja) 2021-08-05
JP6567707B2 (ja) 2019-08-28
WO2011014838A1 (en) 2011-02-03
JP5451884B2 (ja) 2014-03-26
EP2459702B1 (en) 2016-05-04
KR20150044981A (ko) 2015-04-27
SG178194A1 (en) 2012-03-29
KR20170081747A (ko) 2017-07-12
CA2769361A1 (en) 2011-02-03
US20140329294A1 (en) 2014-11-06
KR20150138425A (ko) 2015-12-09
NZ597727A (en) 2013-08-30
JP5936232B2 (ja) 2016-06-22
US20170051270A1 (en) 2017-02-23
BR112012002151A2 (pt) 2020-03-03
KR20120046301A (ko) 2012-05-09
US20190071660A1 (en) 2019-03-07
IN2012DN00801A (es) 2015-06-26
US9441216B2 (en) 2016-09-13
KR101756354B1 (ko) 2017-07-26
JP2016144471A (ja) 2016-08-12
US10072254B2 (en) 2018-09-11
JP2013500724A (ja) 2013-01-10
JP6883071B2 (ja) 2021-06-09
JP2014061005A (ja) 2014-04-10
PL2459702T3 (pl) 2016-12-30
US9127265B2 (en) 2015-09-08

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