CO6501191A2 - Medio de cultivo celular para expresión de la proteina adamts - Google Patents

Medio de cultivo celular para expresión de la proteina adamts

Info

Publication number
CO6501191A2
CO6501191A2 CO12033821A CO12033821A CO6501191A2 CO 6501191 A2 CO6501191 A2 CO 6501191A2 CO 12033821 A CO12033821 A CO 12033821A CO 12033821 A CO12033821 A CO 12033821A CO 6501191 A2 CO6501191 A2 CO 6501191A2
Authority
CO
Colombia
Prior art keywords
expression
adamts
adamts protein
cellular media
methods
Prior art date
Application number
CO12033821A
Other languages
English (en)
Inventor
Leopold Grillberger
Alexandra Spenger
Meinhard Hasslacher
Meinhard Grillberger
Manfred Reiter
Original Assignee
Baxter Int
Baxter Healthcare Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Int, Baxter Healthcare Sa filed Critical Baxter Int
Publication of CO6501191A2 publication Critical patent/CO6501191A2/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Reproductive Health (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)

Abstract

La presente invención proporciona medios de cultivo que son útiles para la expresión de las proteínas ADAMTS, tal como la ADAMTS13. Se proporcionan, además, métodos para la expresión y purificación de proteínas ADAMTS. En algunas modalidades, los medios y métodos de la invención son útiles para la expresión de proteínas ADAMTS con actividades específicas elevadas. Se proporcionan, además, ADAMTS, por ejemplo, ADAMTS 13, composiciones de proteína con actividades específicas elevadas, que se expresan y purificados de acuerdo con los métodos proporcionados en la presente descripción.
CO12033821A 2009-07-31 2012-02-27 Medio de cultivo celular para expresión de la proteina adamts CO6501191A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US23047709P 2009-07-31 2009-07-31

Publications (1)

Publication Number Publication Date
CO6501191A2 true CO6501191A2 (es) 2012-08-15

Family

ID=42668009

Family Applications (1)

Application Number Title Priority Date Filing Date
CO12033821A CO6501191A2 (es) 2009-07-31 2012-02-27 Medio de cultivo celular para expresión de la proteina adamts

Country Status (22)

Country Link
US (7) US8313926B2 (es)
EP (1) EP2459702B1 (es)
JP (5) JP5451884B2 (es)
KR (5) KR101574056B1 (es)
CN (2) CN102549145B (es)
AU (1) AU2010278751B2 (es)
BR (2) BR112012002151B1 (es)
CA (1) CA2769361A1 (es)
CO (1) CO6501191A2 (es)
EA (1) EA023193B1 (es)
ES (1) ES2583258T3 (es)
HK (1) HK1171790A1 (es)
HR (1) HRP20160783T1 (es)
HU (1) HUE029110T2 (es)
IN (1) IN2012DN00801A (es)
MX (2) MX2012001261A (es)
NZ (1) NZ597727A (es)
PL (1) PL2459702T3 (es)
PT (1) PT2459702T (es)
SG (1) SG178194A1 (es)
SI (1) SI2459702T1 (es)
WO (1) WO2011014838A1 (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101574056B1 (ko) * 2009-07-31 2015-12-02 백스터 인터내셔널 인코포레이티드 Ａｄａｍｔｓ 단백질 발현을 위한 세포 배양 배지
TWI670073B (zh) * 2010-07-08 2019-09-01 美商巴克斯歐塔公司 在細胞培養物中生產重組高分子量ｖＷＦ的方法
KR102558247B1 (ko) 2011-07-01 2023-07-24 암젠 인크 포유동물 세포 배양
WO2014062535A1 (en) 2012-10-15 2014-04-24 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
US9611455B2 (en) 2013-03-13 2017-04-04 Merck Sharp & Dohme Corp. Adapted lepidopteran insect cells for the production of recombinant proteins
US9217168B2 (en) 2013-03-14 2015-12-22 Momenta Pharmaceuticals, Inc. Methods of cell culture
AR095196A1 (es) * 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
KR102418824B1 (ko) * 2013-10-14 2022-07-11 아레스 트레이딩 에스.아. 포유동물의 고성능 유가식 배양을 위한 신규 배지
ES2909630T3 (es) 2014-06-04 2022-05-09 Amgen Inc Métodos de recolección en cultivos de células de mamífero
KR101867134B1 (ko) * 2015-03-23 2018-06-12 한양대학교 산학협력단 포유류 세포를 이용하여 목적 물질을 고효율로 생산하기 위한 세포 배양 배지, 이를 이용한 세포 배양 방법 및 목적 물질의 생산 방법
EP3298138B1 (en) * 2015-05-22 2022-08-10 DuPont Nutrition Biosciences ApS Acetolactate decarboxylase
TW202340452A (zh) 2015-08-04 2023-10-16 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法
MX2019001255A (es) 2016-08-04 2019-05-20 Baxalta Inc Uso de una desintegrina y metaloproteinasa con motivo tipo 1 de trombospondina, miembro-13 (adamts13) para tratar, mejorar y/o prevenir crisis vaso-oclusiva en enfermedad de celulas falciformes, lesion pulmonar aguda y/o sindrome de insuficiencia respiratoria aguda.
SG11201903890QA (en) * 2016-11-04 2019-05-30 Baxalta Inc Adeno-associated virus purification methods
SG11201912548XA (en) * 2017-07-06 2020-01-30 Regeneron Pharma Cell culture process for making a glycoprotein
KR20200132932A (ko) * 2018-03-16 2020-11-25 젠자임 코포레이션 생산용 생물 반응기에서의 세포 생존력을 개선하는 방법
CA3103443A1 (en) * 2018-06-18 2019-12-26 Synthetic Biologics, Inc. Methods of making alkaline phosphatase agents
WO2021112927A1 (en) 2019-12-06 2021-06-10 Regeneron Pharmaceuticals, Inc. Anti-vegf protein compositions and methods for producing the same
KR20230005192A (ko) 2020-04-02 2023-01-09 다케다 야쿠힌 고교 가부시키가이샤 Adamts13 변이체, 조성물 및 그의 용도
WO2021226444A2 (en) 2020-05-08 2021-11-11 Regeneron Pharmaceuticals, Inc. Vegf traps and mini-traps and methods for treating ocular disorders and cancer
WO2021234458A1 (en) 2020-05-22 2021-11-25 Takeda Pharmaceutical Company Limited Adamts13 compositions and methods for treating and diagnosing complications of coronavirus disease
WO2021246557A1 (ko) * 2020-06-05 2021-12-09 주식회사 프로앱텍 특정한 갯수의 알부민이 연결된 요산산화효소-알부민 복합체 및 이의 제조 방법
CN111808822B (zh) * 2020-07-02 2024-01-12 北京百普赛斯生物科技股份有限公司 细胞培养用补料液及提高重组hek293细胞蛋白表达量的方法

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US5139941A (en) 1985-10-31 1992-08-18 University Of Florida Research Foundation, Inc. AAV transduction vectors
US5250421A (en) 1986-01-03 1993-10-05 Genetics Institute, Inc. Method for producing factor VIII:C-type proteins
CZ280901B6 (cs) 1988-10-06 1996-05-15 Hideaki Yamada Způsob kultivace bakterií
JPH0753104B2 (ja) * 1989-02-28 1995-06-07 秀明 山田 細菌の培養法
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
ATE542891T1 (de) 1994-11-10 2012-02-15 Baxter Healthcare Sa Verfahren zur herstellung von biologischen produkten in protein-freier kultur
JP3649249B2 (ja) 1995-02-23 2005-05-18 クエスト・インターナショナル・サービシーズ・ビー・ブイ 組織および細胞培養用ペプチド
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
JP2000517188A (ja) 1996-08-30 2000-12-26 ライフ テクノロジーズ，インコーポレイテッド 無血清哺乳動物細胞培養培地およびその使用
IT1285158B1 (it) 1996-09-17 1998-06-03 Angeletti P Ist Richerche Bio Polipeptidi solubili con l'attivita' di serino-proteasi di ns3 del virus dell'epatite c, e procedimento per la loro preparazione e il
DE69717650T2 (de) 1996-10-07 2003-09-18 Solutia Inc., St. Louis Verfahren zur hydroxylierung von benzol
DE69738806D1 (de) 1996-10-10 2008-08-14 Invitrogen Corp Tierzellkulturmedium mit pflanzlichen nährstoffen
US5804420A (en) 1997-04-18 1998-09-08 Bayer Corporation Preparation of recombinant Factor VIII in a protein free medium
AT407255B (de) 1997-06-20 2001-02-26 Immuno Ag Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
US6406909B1 (en) 1998-07-10 2002-06-18 Chugai Seiyaku Kabushiki Kaisha Serum-free medium for culturing animal cells
AT409379B (de) 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
US6926894B2 (en) 2000-11-22 2005-08-09 Baxter Aktiengesellschaft Composition exhibiting a von willebrand factor (vWF) protease activity comprising a polypeptide chain with the amino acid sequence AAGGILHLELLV
EP1576148B1 (en) 2002-12-23 2012-10-03 FUJIFILM Manufacturing Europe B.V. Process for coating cell-culture support
CA2562683A1 (en) * 2004-04-16 2005-12-08 Wyeth Adamts-8 proteins and uses thereof
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
JP2007174948A (ja) * 2005-12-27 2007-07-12 Noritsu Koki Co Ltd 酵素水製造装置
JP4880997B2 (ja) * 2005-12-28 2012-02-22 アルフレッサファーマ株式会社 Ａｄａｍｔｓ１３の安定化方法
PL3121266T3 (pl) 2006-01-04 2020-07-13 Baxalta Incorporated Wolne od oligopeptydów pożywki do hodowli komórkowej
CN101611268B (zh) * 2006-12-22 2013-06-12 斯普灵格开利有限公司 用于空调单元的新鲜空气控制杆
US20090067805A1 (en) 2007-09-10 2009-03-12 Victor Company Of Japan, Limited Recording and reproducing device
US9359629B2 (en) * 2007-12-27 2016-06-07 Baxalta Incorporated Cell culture processes
KR101574056B1 (ko) * 2009-07-31 2015-12-02 백스터 인터내셔널 인코포레이티드 Ａｄａｍｔｓ 단백질 발현을 위한 세포 배양 배지

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Publication number Publication date
CN104962539B (zh) 2019-09-17
US20110086413A1 (en) 2011-04-14
EA023193B1 (ru) 2016-05-31
AU2010278751B2 (en) 2014-05-15
BR122021005965B1 (pt) 2022-01-25
KR101808496B1 (ko) 2017-12-12
CN102549145B (zh) 2015-05-20
US20150329848A1 (en) 2015-11-19
KR101573972B1 (ko) 2015-12-02
MX344970B (es) 2017-01-12
US11254921B2 (en) 2022-02-22
KR101574056B1 (ko) 2015-12-02
PT2459702T (pt) 2016-08-02
JP2019201661A (ja) 2019-11-28
JP6511007B2 (ja) 2019-05-08
MX2012001261A (es) 2012-05-22
US8759026B2 (en) 2014-06-24
EP2459702A1 (en) 2012-06-06
ES2583258T3 (es) 2016-09-20
JP7083059B2 (ja) 2022-06-09
HK1171790A1 (zh) 2013-04-05
CN104962539A (zh) 2015-10-07
HRP20160783T1 (hr) 2016-08-12
US8313926B2 (en) 2012-11-20
EA201270224A1 (ru) 2012-09-28
KR101912552B1 (ko) 2018-10-26
BR112012002151B1 (pt) 2021-07-06
AU2010278751A1 (en) 2012-02-09
CN102549145A (zh) 2012-07-04
US20130071906A1 (en) 2013-03-21
HUE029110T2 (en) 2017-02-28
SI2459702T1 (sl) 2016-11-30
US10724024B2 (en) 2020-07-28
US20200308566A1 (en) 2020-10-01
JP2018093881A (ja) 2018-06-21
KR20170140411A (ko) 2017-12-20
JP2021112210A (ja) 2021-08-05
JP6567707B2 (ja) 2019-08-28
WO2011014838A1 (en) 2011-02-03
JP5451884B2 (ja) 2014-03-26
EP2459702B1 (en) 2016-05-04
KR20150044981A (ko) 2015-04-27
SG178194A1 (en) 2012-03-29
KR20170081747A (ko) 2017-07-12
CA2769361A1 (en) 2011-02-03
US20140329294A1 (en) 2014-11-06
KR20150138425A (ko) 2015-12-09
NZ597727A (en) 2013-08-30
JP5936232B2 (ja) 2016-06-22
US20170051270A1 (en) 2017-02-23
BR112012002151A2 (pt) 2020-03-03
KR20120046301A (ko) 2012-05-09
US20190071660A1 (en) 2019-03-07
IN2012DN00801A (es) 2015-06-26
US9441216B2 (en) 2016-09-13
KR101756354B1 (ko) 2017-07-26
JP2016144471A (ja) 2016-08-12
US10072254B2 (en) 2018-09-11
JP2013500724A (ja) 2013-01-10
JP6883071B2 (ja) 2021-06-09
JP2014061005A (ja) 2014-04-10
PL2459702T3 (pl) 2016-12-30
US9127265B2 (en) 2015-09-08

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