NZ597727A - Cell culture medium for adamts protein expression - Google Patents

Cell culture medium for adamts protein expression

Info

Publication number
NZ597727A
NZ597727A NZ597727A NZ59772710A NZ597727A NZ 597727 A NZ597727 A NZ 597727A NZ 597727 A NZ597727 A NZ 597727A NZ 59772710 A NZ59772710 A NZ 59772710A NZ 597727 A NZ597727 A NZ 597727A
Authority
NZ
New Zealand
Prior art keywords
culture medium
cell culture
protein expression
adamts protein
adamts
Prior art date
Application number
NZ597727A
Inventor
Rana Grillberger
Leopold Grillberger
Alexandra Spenger
Meinhard Hasslacher
Manfred Reiter
Original Assignee
Baxter Int
Baxter Healthcare Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Int, Baxter Healthcare Sa filed Critical Baxter Int
Publication of NZ597727A publication Critical patent/NZ597727A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Abstract

Patent No. 597727 Disclosed is a method for expressing a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) protein, the method comprising culturing a cell harboring a nucleic acid encoding an ADAMTS protein in a culture medium comprising at least 3 ?M zinc.
NZ597727A 2009-07-31 2010-07-30 Cell culture medium for adamts protein expression NZ597727A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23047709P 2009-07-31 2009-07-31
PCT/US2010/044020 WO2011014838A1 (en) 2009-07-31 2010-07-30 Cell culture medium for adamts protein expression

Publications (1)

Publication Number Publication Date
NZ597727A true NZ597727A (en) 2013-08-30

Family

ID=42668009

Family Applications (1)

Application Number Title Priority Date Filing Date
NZ597727A NZ597727A (en) 2009-07-31 2010-07-30 Cell culture medium for adamts protein expression

Country Status (22)

Country Link
US (7) US8313926B2 (en)
EP (1) EP2459702B1 (en)
JP (5) JP5451884B2 (en)
KR (5) KR101574056B1 (en)
CN (2) CN102549145B (en)
AU (1) AU2010278751B2 (en)
BR (2) BR122021005965B1 (en)
CA (1) CA2769361A1 (en)
CO (1) CO6501191A2 (en)
EA (1) EA023193B1 (en)
ES (1) ES2583258T3 (en)
HK (1) HK1171790A1 (en)
HR (1) HRP20160783T1 (en)
HU (1) HUE029110T2 (en)
IN (1) IN2012DN00801A (en)
MX (2) MX2012001261A (en)
NZ (1) NZ597727A (en)
PL (1) PL2459702T3 (en)
PT (1) PT2459702T (en)
SG (1) SG178194A1 (en)
SI (1) SI2459702T1 (en)
WO (1) WO2011014838A1 (en)

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WO2021198781A2 (en) 2020-04-02 2021-10-07 Takeda Pharmaceutical Company Limited Adamts13 variant, compositions, and uses thereof
CN115835904A (en) 2020-05-22 2023-03-21 武田药品工业有限公司 ADAMTS13 compositions and methods for the treatment and diagnosis of coronary viral disease complications
CN111808822B (en) * 2020-07-02 2024-01-12 北京百普赛斯生物科技股份有限公司 Feed supplement liquid for cell culture and method for improving recombinant HEK293 cell protein expression quantity

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Also Published As

Publication number Publication date
US20150329848A1 (en) 2015-11-19
US9127265B2 (en) 2015-09-08
US20190071660A1 (en) 2019-03-07
AU2010278751B2 (en) 2014-05-15
HK1171790A1 (en) 2013-04-05
KR20150138425A (en) 2015-12-09
EA201270224A1 (en) 2012-09-28
PL2459702T3 (en) 2016-12-30
SI2459702T1 (en) 2016-11-30
US10724024B2 (en) 2020-07-28
US8759026B2 (en) 2014-06-24
JP2016144471A (en) 2016-08-12
JP6883071B2 (en) 2021-06-09
JP2013500724A (en) 2013-01-10
EA023193B1 (en) 2016-05-31
US20170051270A1 (en) 2017-02-23
CN104962539B (en) 2019-09-17
BR122021005965B1 (en) 2022-01-25
US20200308566A1 (en) 2020-10-01
MX344970B (en) 2017-01-12
JP2014061005A (en) 2014-04-10
HUE029110T2 (en) 2017-02-28
AU2010278751A1 (en) 2012-02-09
US20130071906A1 (en) 2013-03-21
JP6511007B2 (en) 2019-05-08
EP2459702A1 (en) 2012-06-06
MX2012001261A (en) 2012-05-22
US9441216B2 (en) 2016-09-13
US10072254B2 (en) 2018-09-11
CN104962539A (en) 2015-10-07
HRP20160783T1 (en) 2016-08-12
US11254921B2 (en) 2022-02-22
CN102549145A (en) 2012-07-04
JP2019201661A (en) 2019-11-28
JP5451884B2 (en) 2014-03-26
KR101912552B1 (en) 2018-10-26
JP6567707B2 (en) 2019-08-28
JP2018093881A (en) 2018-06-21
US8313926B2 (en) 2012-11-20
JP7083059B2 (en) 2022-06-09
IN2012DN00801A (en) 2015-06-26
KR101756354B1 (en) 2017-07-26
ES2583258T3 (en) 2016-09-20
BR112012002151A2 (en) 2020-03-03
KR20170140411A (en) 2017-12-20
KR101808496B1 (en) 2017-12-12
KR20170081747A (en) 2017-07-12
KR20120046301A (en) 2012-05-09
KR20150044981A (en) 2015-04-27
CO6501191A2 (en) 2012-08-15
CA2769361A1 (en) 2011-02-03
US20110086413A1 (en) 2011-04-14
WO2011014838A1 (en) 2011-02-03
BR112012002151B1 (en) 2021-07-06
JP2021112210A (en) 2021-08-05
JP5936232B2 (en) 2016-06-22
PT2459702T (en) 2016-08-02
CN102549145B (en) 2015-05-20
KR101574056B1 (en) 2015-12-02
SG178194A1 (en) 2012-03-29
US20140329294A1 (en) 2014-11-06
EP2459702B1 (en) 2016-05-04
KR101573972B1 (en) 2015-12-02

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