JP6957738B2 - スルファターゼタンパク質を精製する方法 - Google Patents
スルファターゼタンパク質を精製する方法 Download PDFInfo
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- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
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- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
- B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
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- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
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- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
- B01J39/04—Processes using organic exchangers
- B01J39/05—Processes using organic exchangers in the strongly acidic form
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- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/04—Processes using organic exchangers
- B01J41/05—Processes using organic exchangers in the strongly basic form
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
- B01J41/04—Processes using organic exchangers
- B01J41/07—Processes using organic exchangers in the weakly basic form
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y310/00—Hydrolases acting on sulfur-nitrogen bonds (3.10)
- C12Y310/01—Hydrolases acting on sulfur-nitrogen bonds (3.10) acting on sulfur-nitrogen bonds (3.10.1)
- C12Y310/01001—N-Sulfoglucosamine sulfohydrolase (3.10.1.1)
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3847—Multimodal interactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/06—Sulfuric ester hydrolases (3.1.6)
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Description
すべてのクロマトグラフィーステップは、室温で行われた。図11は、実施例1による精製方法のフローチャートを示す。クロマトグラフィー装置:Akta Explorer、Akta Purifier 100、またはGE HealthcareのAkta Pilot。
すべてのクロマトグラフィーステップは、室温で行われた。図13は、実施例2による精製方法のフローチャートを示す。クロマトグラフィー装置:Akta Explorer、またはAkta Purifier 100、またはGE HealthcareのAkta Pilot。
すべてのクロマトグラフィーステップは、室温で行われた。図15は、実施例3による精製方法のフローチャートを示す。クロマトグラフィー装置:Akta Explorer、またはAkta Purifier 100、またはGE HealthcareのAkta Pilot。
生物学的活性は、株式会社グリーンクロスの社内分析によって決定された。参照標準SGSHタンパク質としては、CF(R&D systems、Cat.# 8380−SU−020)が使用された。
Claims (14)
- (a)一つまたは複数の不純物を含むスルファターゼ含有溶液を提供するステップと、
(b)金属アフィニティー・クロマトグラフィー樹脂を用いて、スルファターゼ含有溶液の第1クロマトグラフィー分離を行うステップと、
(c)陽イオン交換クロマトグラフィー樹脂を用いて、第2クロマトグラフィー分離を行うステップと、
(d)陰イオン交換クロマトグラフィー樹脂を用いて、最終のクロマトグラフィー分離を行い、前記不純物を除去するステップとを含み、
前記スルファターゼは、ヘパラン−N−スルファターゼ(heparan−N−sulfatase)である、スルファターゼを精製する方法。 - 前記金属アフィニティー・クロマトグラフィー樹脂は、2価の金属陽イオンに荷電される、請求項1に記載の方法。
- 前記2価の金属は亜鉛である、請求項2に記載の方法。
- 前記陽イオン交換クロマトグラフィー樹脂は、強陽イオン交換クロマトグラフィー樹脂およびマルチモードの陽イオン交換クロマトグラフィー樹脂からなる群より選択される、請求項1に記載の方法。
- 陽イオン交換クロマトグラフィー樹脂を用いて、第3クロマトグラフィー分離を行うステップをさらに含み、
前記第2クロマトグラフィー分離のステップで使用される樹脂は、強陽イオン交換クロマトグラフィー樹脂であり、
前記第3クロマトグラフィー分離のステップで使用される樹脂は、マルチモードの陽イオン交換クロマトグラフィー樹脂である、請求項1に記載の方法。 - 前記陰イオン交換クロマトグラフィー樹脂は、強陰イオン交換クロマトグラフィー樹脂および弱陰イオン交換クロマトグラフィー樹脂からなる群より選択される、請求項1に記載の方法。
- 前記スルファターゼは、活性サイトにカルシウムイオン、鉄イオンおよび亜鉛イオンからなる群より選択される金属イオンを有する、請求項1に記載の方法。
- 低pHウイルス不活性化のステップをさらに含む、請求項1に記載の方法。
- 前記低pHウイルス不活性化のステップは、第1クロマトグラフィー分離ステップの後および第2クロマトグラフィー分離ステップの前に行われるか、又は、
第2クロマトグラフィー分離ステップの後および最終のクロマトグラフィー分離ステップの前に行われる、請求項8に記載の方法。 - 低pHウイルス不活性化をさらに含む、請求項5に記載の方法。
- 前記低pHウイルス不活性化のステップは、第1クロマトグラフィー分離ステップの後および第2クロマトグラフィー分離ステップの前に行われるか、又は、
第2クロマトグラフィー分離ステップの後および第3クロマトグラフィー分離ステップの前に行われる、請求項10に記載の方法。 - ウイルス濾過ステップをさらに含む、請求項1に記載の方法。
- 前記ウイルス濾過ステップは、最終のクロマトグラフィー分離ステップの後に行われる、請求項12に記載の方法。
- 前記スルファターゼ含有溶液は、細胞培養収穫物および部分的に精製された中間溶液からなる群より選択される、請求項1に記載の方法。
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PCT/KR2017/009561 WO2019045149A1 (en) | 2017-08-31 | 2017-08-31 | PROCESS FOR PURIFYING SULFATASE PROTEIN |
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JP6957738B2 true JP6957738B2 (ja) | 2021-11-02 |
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US (1) | US11584777B2 (ja) |
EP (1) | EP3676375A4 (ja) |
JP (1) | JP6957738B2 (ja) |
KR (1) | KR102286260B1 (ja) |
CN (1) | CN111065735B (ja) |
WO (1) | WO2019045149A1 (ja) |
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KR20240039642A (ko) * | 2022-09-19 | 2024-03-27 | 주식회사 녹십자 | 신규 헤파란-n-설파타아제의 정제 방법 |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
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US20040161837A1 (en) | 1995-08-02 | 2004-08-19 | Reuser Arnold J. | Methods of purifying human acid alpha-glucosidase |
DE19816395A1 (de) | 1998-04-03 | 1999-10-07 | Metagen Gesellschaft Fuer Genomforschung Mbh | Menschliche Nukleinsäuresequenzen aus Ovar-Normalgewebe |
SE9904197D0 (sv) * | 1999-11-22 | 1999-11-22 | Amersham Pharm Biotech Ab | A method for anion exchange adsorption on matrices carrying mixed mode ligands |
US6866844B2 (en) | 2002-11-07 | 2005-03-15 | Biomarin Pharmaceutical Inc. | Precursor N-acetylgalactosamine-4-sulfatase, methods of treatment using said enzyme and methods for producing and purifying said enzyme |
JP4606712B2 (ja) | 2003-01-08 | 2011-01-05 | マサチューセッツ インスティテュート オブ テクノロジー | 2−oスルファターゼ組成物および関連の方法 |
NZ576986A (en) | 2004-01-30 | 2010-12-24 | Shire Pharmaceuticals Ireland Ltd | Production and purification of recombinant arylsulfatase A |
NZ588903A (en) | 2006-04-04 | 2012-08-31 | Zymenex As | A process for concentration of galactosycerebrosidase or porphobuilinogen deaminase |
CA2674493C (en) | 2007-01-05 | 2017-05-09 | Massachusetts Institute Of Technology | Compositions of and methods of using sulfatases from flavobacterium heparinum |
EP2245145B1 (en) | 2008-01-18 | 2016-12-14 | BioMarin Pharmaceutical Inc. | Manufacture of active highly phosphorylated human lysosomal sulfatase enzymes and uses thereof |
KR20160104740A (ko) | 2008-06-24 | 2016-09-05 | 옥타파마 아게 | 응고 인자 viii을 정제하는 방법 |
KR20140005842A (ko) * | 2010-06-25 | 2014-01-15 | 샤이어 휴먼 지네틱 테라피즈 인크. | 헤파란 n-설파타제의 cns 전달을 위한 방법들 및 조성물들 |
AR082319A1 (es) | 2010-07-22 | 2012-11-28 | Biomarin Pharm Inc | Produccion de una n-acetilgalactosamina-6-sulfatasa humana activa altamente fosforilada y sus usos |
WO2012101671A1 (en) | 2011-01-25 | 2012-08-02 | Jcr Pharmaceuticals Co., Ltd. | Method for production of human recombinant iduronate 2-sulfatase |
JP6018572B2 (ja) * | 2011-01-25 | 2016-11-02 | Jcrファーマ株式会社 | 組換えヒトイズロン酸−2−スルファターゼの製造方法 |
TW201307563A (zh) | 2011-05-19 | 2013-02-16 | Shire Human Genetic Therapies | 純化乙醯肝素-n-硫酸酯酶之方法 |
US11407984B2 (en) | 2013-01-09 | 2022-08-09 | Takeda Pharmaceutical Company Limited | Methods for purification of arysulfatase A |
HUE057119T2 (hu) | 2013-05-06 | 2022-04-28 | Sanofi Sa | Folyamatos többlépéses eljárás antitestek tisztítására |
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- 2017-08-31 EP EP17923077.6A patent/EP3676375A4/en active Pending
- 2017-08-31 US US16/643,355 patent/US11584777B2/en active Active
- 2017-08-31 JP JP2020512518A patent/JP6957738B2/ja active Active
- 2017-08-31 CN CN201780094439.7A patent/CN111065735B/zh active Active
- 2017-08-31 WO PCT/KR2017/009561 patent/WO2019045149A1/en unknown
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Publication number | Publication date |
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EP3676375A1 (en) | 2020-07-08 |
KR20200022052A (ko) | 2020-03-02 |
WO2019045149A1 (en) | 2019-03-07 |
CN111065735B (zh) | 2023-07-07 |
JP2020532301A (ja) | 2020-11-12 |
EP3676375A4 (en) | 2021-04-07 |
KR102286260B1 (ko) | 2021-08-05 |
CN111065735A (zh) | 2020-04-24 |
US20200361985A1 (en) | 2020-11-19 |
US11584777B2 (en) | 2023-02-21 |
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