WO2012028753A8 - Procedimiento para la producción y purificación de proteínas recombinantes en plantas - Google Patents
Procedimiento para la producción y purificación de proteínas recombinantes en plantas Download PDFInfo
- Publication number
- WO2012028753A8 WO2012028753A8 PCT/ES2011/000266 ES2011000266W WO2012028753A8 WO 2012028753 A8 WO2012028753 A8 WO 2012028753A8 ES 2011000266 W ES2011000266 W ES 2011000266W WO 2012028753 A8 WO2012028753 A8 WO 2012028753A8
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- plants
- recombinant proteins
- producing
- purifying recombinant
- protease
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 108091005804 Peptidases Proteins 0.000 abstract 2
- 239000004365 Protease Substances 0.000 abstract 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 229920002472 Starch Polymers 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 239000008187 granular material Substances 0.000 abstract 1
- 238000000265 homogenisation Methods 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 235000019698 starch Nutrition 0.000 abstract 1
- 239000008107 starch Substances 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
- 238000011282 treatment Methods 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8221—Transit peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
La invención se refiere a un procedimiento para la producción y purificación de proteínas recombinantes en plantas, mediante la producción de plantas transgénicas que expresan las proteínas recombinantes fusionadas con proteínas asociadas al gránulo de almidón a través de una secuencia reconocida por una proteasa. Este procedimiento permite la purificación de la proteína mediante etapas simples de homogeneización, centrifugación y tratamiento con la proteasa. Así mismo, la invención se refiere a los vectores, hospedadores, células y plantas empleados en dicho procedimiento.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP11821149.9A EP2626363A1 (en) | 2010-08-31 | 2011-08-25 | Method for producing and purifying recombinant proteins in plants |
TW100130913A TW201224143A (en) | 2010-08-31 | 2011-08-29 | Method for producing and purifying recombinant proteins in plants |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ESI201001115 | 2010-08-31 | ||
ESP201001115 | 2010-08-31 | ||
ES201001115A ES2377617B1 (es) | 2010-08-31 | 2010-08-31 | Procedimiento para la producción y purificación de proteínas recombinantes en plantas. |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2012028753A1 WO2012028753A1 (es) | 2012-03-08 |
WO2012028753A8 true WO2012028753A8 (es) | 2013-05-10 |
Family
ID=45772199
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/ES2011/000266 WO2012028753A1 (es) | 2010-08-31 | 2011-08-25 | Procedimiento para la producción y purificación de proteínas recombinantes en plantas |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP2626363A1 (es) |
ES (1) | ES2377617B1 (es) |
TW (1) | TW201224143A (es) |
WO (1) | WO2012028753A1 (es) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3808854A1 (de) * | 2019-10-17 | 2021-04-21 | eleva GmbH | Verfahren zur gewinnung von material von pflanzenzelloberflächen |
CN110791519A (zh) * | 2019-11-20 | 2020-02-14 | 四川农业大学 | 玉米phol特异蛋白及特异分解的鉴定方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5137819A (en) | 1988-07-08 | 1992-08-11 | University Of British Columbia | Cellulose binding fusion proteins for immobilization and purification of polypeptides |
RU2268301C9 (ru) * | 1996-09-30 | 2006-07-20 | Басф Плант Сайенс Гмбх | Инкапсулирование полипептидов в матрицу крахмала |
FR2793806B1 (fr) * | 1999-05-21 | 2003-04-25 | Centre Nat Rech Scient | Grains d'amidon contenant un polypeptide recombinant d'interet, leur procede d'obtention, et leurs utilisations |
US7135619B1 (en) * | 1999-06-11 | 2006-11-14 | Wageningen Universiteit | Expression in plants of starch binding domains and/or of protein-fusions containing starch binding domains |
-
2010
- 2010-08-31 ES ES201001115A patent/ES2377617B1/es active Active
-
2011
- 2011-08-25 EP EP11821149.9A patent/EP2626363A1/en not_active Withdrawn
- 2011-08-25 WO PCT/ES2011/000266 patent/WO2012028753A1/es active Application Filing
- 2011-08-29 TW TW100130913A patent/TW201224143A/zh unknown
Also Published As
Publication number | Publication date |
---|---|
WO2012028753A1 (es) | 2012-03-08 |
TW201224143A (en) | 2012-06-16 |
EP2626363A1 (en) | 2013-08-14 |
ES2377617B1 (es) | 2012-11-23 |
ES2377617A1 (es) | 2012-03-29 |
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