AR089362A1 - Metodos para mejorar las etapas de purificacion de proteinas descendentes utilizando cromatografia de membranas de intercambio ionico - Google Patents
Metodos para mejorar las etapas de purificacion de proteinas descendentes utilizando cromatografia de membranas de intercambio ionicoInfo
- Publication number
- AR089362A1 AR089362A1 ARP120104862A ARP120104862A AR089362A1 AR 089362 A1 AR089362 A1 AR 089362A1 AR P120104862 A ARP120104862 A AR P120104862A AR P120104862 A ARP120104862 A AR P120104862A AR 089362 A1 AR089362 A1 AR 089362A1
- Authority
- AR
- Argentina
- Prior art keywords
- polypeptide
- membrane
- ion exchange
- purification
- effluent
- Prior art date
Links
- 239000003014 ion exchange membrane Substances 0.000 title abstract 4
- 238000000746 purification Methods 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 238000004587 chromatography analysis Methods 0.000 title 1
- 229920001184 polypeptide Polymers 0.000 abstract 9
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 9
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 9
- 239000012528 membrane Substances 0.000 abstract 5
- 239000000356 contaminant Substances 0.000 abstract 3
- 238000011210 chromatographic step Methods 0.000 abstract 1
- 238000004255 ion exchange chromatography Methods 0.000 abstract 1
- 150000002500 ions Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
Abstract
Métodos para mejorar la eficacia de las etapas de la cromatografía descendente para la purificación de proteínas que comprende: (a) pasar una composición que comprende un polipéptido de interés y diversos contaminantes a través de una membrana de intercambio iónico, en donde el polipéptido y la membrana tienen carga opuesta, en condiciones operativas compuestas por un tampón que tiene un pH lo suficientemente diferente del pI del polipéptido como para mejorar la carga del polipéptido y una fuerza iónica baja eficaz para evitar el blindaje de las cargas por los iones del tampón, lo que provoca que la membrana se una al polipéptido y al menos a un contaminante; (b) sobrecargar la membrana de intercambio iónico de modo que al menos un contaminante permanezca unido a la membrana mientras el polipéptido de interés se encuentra principalmente en el efluente; (c) recolectar el efluente de la membrana de intercambio iónico que comprende el polipéptido de interés; (d) someter el efluente de la membrana que comprende el polipéptido de interés a una etapa de purificación de carga similar a la membrana anterior; y (e) recuperar el polipéptido purificado del efluente de la etapa de purificación por cromatografía de intercambio iónico cargada.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161579285P | 2011-12-22 | 2011-12-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR089362A1 true AR089362A1 (es) | 2014-08-20 |
Family
ID=47430154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP120104862A AR089362A1 (es) | 2011-12-22 | 2012-12-20 | Metodos para mejorar las etapas de purificacion de proteinas descendentes utilizando cromatografia de membranas de intercambio ionico |
Country Status (22)
| Country | Link |
|---|---|
| US (2) | US10364268B2 (es) |
| EP (2) | EP3492486A1 (es) |
| JP (5) | JP6605202B2 (es) |
| KR (3) | KR20200008022A (es) |
| CN (2) | CN104125963B (es) |
| AR (1) | AR089362A1 (es) |
| AU (3) | AU2012355356B2 (es) |
| BR (1) | BR112014015101A8 (es) |
| CA (1) | CA2859376C (es) |
| ES (1) | ES2697676T3 (es) |
| HK (1) | HK1203526A1 (es) |
| HR (1) | HRP20181846T1 (es) |
| IL (2) | IL267585B (es) |
| MX (2) | MX360453B (es) |
| MY (1) | MY172426A (es) |
| PL (1) | PL2794635T3 (es) |
| RU (1) | RU2648999C2 (es) |
| SG (1) | SG11201403437TA (es) |
| SI (1) | SI2794635T1 (es) |
| TR (1) | TR201815709T4 (es) |
| WO (1) | WO2013096322A1 (es) |
| ZA (2) | ZA201404593B (es) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TR201815709T4 (tr) * | 2011-12-22 | 2018-11-21 | Hoffmann La Roche | İyon değişim membranı kromatografisi. |
| CN110054661A (zh) | 2013-12-12 | 2019-07-26 | Emd密理博公司 | 使用含丙烯酰胺的过滤器分离蛋白 |
| TWI787590B (zh) * | 2013-12-27 | 2022-12-21 | 日商中外製藥股份有限公司 | 等電點低之抗體的精製方法 |
| CN104693303B (zh) * | 2015-02-28 | 2018-03-13 | 苏州金盟生物技术有限公司 | 一种蛋白制品中的核酸去除方法 |
| SG10202101105XA (en) | 2015-08-13 | 2021-03-30 | Amgen Inc | Charged depth filtration of antigen-binding proteins |
| GB201711481D0 (en) | 2017-07-17 | 2017-08-30 | Ucb Biopharma Sprl | Protein purification |
| EP3546475A1 (en) * | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
| US10792618B2 (en) | 2018-06-19 | 2020-10-06 | Sartorius Stedim Biotech Gmbh | Particle separation and/or purification of a fluid |
| GB201815405D0 (en) * | 2018-09-21 | 2018-11-07 | Fujifilm Mfg Europe Bv | Membranes suitable for detecting, filtering and/or purifying biomolecules |
| WO2020128797A1 (en) * | 2018-12-21 | 2020-06-25 | 3M Innovative Properties Company | Method for testing a chromatography device used for ion exchange |
| AR119264A1 (es) * | 2019-06-05 | 2021-12-09 | Genentech Inc | Método para reutilización de cromatografía |
| US11416468B2 (en) * | 2020-07-21 | 2022-08-16 | International Business Machines Corporation | Active-active system index management |
| CN116371011B (zh) * | 2023-05-26 | 2023-08-18 | 湖南杰萃生物技术有限公司 | 一种从桑叶中提取黄酮和生物碱的方法 |
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2012
- 2012-12-18 TR TR2018/15709T patent/TR201815709T4/tr unknown
- 2012-12-18 CN CN201280070369.9A patent/CN104125963B/zh active Active
- 2012-12-18 MX MX2014007622A patent/MX360453B/es active IP Right Grant
- 2012-12-18 PL PL12806322T patent/PL2794635T3/pl unknown
- 2012-12-18 KR KR1020207000846A patent/KR20200008022A/ko not_active IP Right Cessation
- 2012-12-18 AU AU2012355356A patent/AU2012355356B2/en active Active
- 2012-12-18 IL IL267585A patent/IL267585B/en unknown
- 2012-12-18 SI SI201231443T patent/SI2794635T1/sl unknown
- 2012-12-18 US US14/365,449 patent/US10364268B2/en active Active
- 2012-12-18 RU RU2014130017A patent/RU2648999C2/ru active
- 2012-12-18 KR KR1020147019968A patent/KR102067075B1/ko active IP Right Grant
- 2012-12-18 KR KR1020217005616A patent/KR20210025692A/ko not_active Application Discontinuation
- 2012-12-18 ES ES12806322T patent/ES2697676T3/es active Active
- 2012-12-18 JP JP2014549204A patent/JP6605202B2/ja active Active
- 2012-12-18 EP EP18193475.3A patent/EP3492486A1/en active Pending
- 2012-12-18 CN CN201810818291.3A patent/CN108892706A/zh active Pending
- 2012-12-18 BR BR112014015101A patent/BR112014015101A8/pt not_active Application Discontinuation
- 2012-12-18 WO PCT/US2012/070373 patent/WO2013096322A1/en active Application Filing
- 2012-12-18 MY MYPI2014701665A patent/MY172426A/en unknown
- 2012-12-18 SG SG11201403437TA patent/SG11201403437TA/en unknown
- 2012-12-18 CA CA2859376A patent/CA2859376C/en active Active
- 2012-12-18 EP EP12806322.9A patent/EP2794635B8/en active Active
- 2012-12-20 AR ARP120104862A patent/AR089362A1/es not_active Application Discontinuation
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2014
- 2014-06-16 IL IL233158A patent/IL233158B/en active IP Right Grant
- 2014-06-20 MX MX2018013356A patent/MX2018013356A/es unknown
- 2014-06-23 ZA ZA2014/04593A patent/ZA201404593B/en unknown
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2015
- 2015-04-27 HK HK15104046.9A patent/HK1203526A1/xx unknown
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2018
- 2018-01-10 AU AU2018200194A patent/AU2018200194B2/en active Active
- 2018-02-13 JP JP2018023128A patent/JP2018109024A/ja not_active Withdrawn
- 2018-11-05 HR HRP20181846TT patent/HRP20181846T1/hr unknown
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2019
- 2019-06-06 US US16/433,763 patent/US11945837B2/en active Active
- 2019-06-20 ZA ZA2019/03994A patent/ZA201903994B/en unknown
- 2019-10-24 JP JP2019193276A patent/JP7402011B2/ja active Active
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2020
- 2020-02-14 AU AU2020201070A patent/AU2020201070A1/en not_active Abandoned
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2021
- 2021-06-22 JP JP2021102974A patent/JP7359807B2/ja active Active
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2022
- 2022-03-25 JP JP2022050501A patent/JP2022101557A/ja active Pending
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