EA201590778A1 - ОЧИСТКА КОМБИНИРОВАННОЙ ГАЛАКТОЦЕРЕБРОЗИД-β-ГАЛАКТОЗИДАЗЫ ЧЕЛОВЕКА (rhGALC) - Google Patents
ОЧИСТКА КОМБИНИРОВАННОЙ ГАЛАКТОЦЕРЕБРОЗИД-β-ГАЛАКТОЗИДАЗЫ ЧЕЛОВЕКА (rhGALC)Info
- Publication number
- EA201590778A1 EA201590778A1 EA201590778A EA201590778A EA201590778A1 EA 201590778 A1 EA201590778 A1 EA 201590778A1 EA 201590778 A EA201590778 A EA 201590778A EA 201590778 A EA201590778 A EA 201590778A EA 201590778 A1 EA201590778 A1 EA 201590778A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- galactocerebrozide
- galaktosidase
- man
- cleaning
- combined
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3847—Multimodal interactions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
- B01J20/287—Non-polar phases; Reversed phases
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
- B01J20/288—Polar phases
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
- B01J20/289—Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01046—Galactosylceramidase (3.2.1.46)
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
- B01J2220/52—Sorbents specially adapted for preparative chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Epidemiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Neurosurgery (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Neurology (AREA)
- Psychiatry (AREA)
- Hospice & Palliative Care (AREA)
- Enzymes And Modification Thereof (AREA)
- Biophysics (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Настоящее изобретение относится к способу очистки рекомбинантной галактоцереброзид-β-галактозидазы человека (rhGALC) из клеточной культуры, при котором фракцию указанной клеточной культуры, содержащей rhGALC, подвергают хроматографии на трех различных смолах.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA201270699 | 2012-11-13 | ||
PCT/DK2013/050378 WO2014075688A1 (en) | 2012-11-13 | 2013-11-13 | PURIFICATION OF RECOMBINANT HUMAN GALACTOCEREBROSIDE β-GALACTOSIDASE (rhGALC) |
Publications (2)
Publication Number | Publication Date |
---|---|
EA201590778A1 true EA201590778A1 (ru) | 2016-01-29 |
EA032680B1 EA032680B1 (ru) | 2019-07-31 |
Family
ID=54209221
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA201590778A EA032680B1 (ru) | 2012-11-13 | 2013-11-13 | ОЧИСТКА РЕКОМБИНАНТНОЙ ГАЛАКТОЦЕРЕБРОЗИД-БЕТА-ГАЛАКТОЗИДАЗЫ ЧЕЛОВЕКА (rhGALC) |
Country Status (16)
Country | Link |
---|---|
US (1) | US20150284698A1 (ru) |
EP (1) | EP2920198B1 (ru) |
JP (1) | JP6035427B2 (ru) |
KR (1) | KR101783466B1 (ru) |
CN (1) | CN104854122B (ru) |
AU (1) | AU2013347260B2 (ru) |
BR (1) | BR112015010796B1 (ru) |
CA (1) | CA2890358C (ru) |
EA (1) | EA032680B1 (ru) |
ES (1) | ES2751369T3 (ru) |
HK (1) | HK1213915A1 (ru) |
IL (1) | IL238775B (ru) |
NZ (1) | NZ707753A (ru) |
PL (1) | PL2920198T3 (ru) |
WO (1) | WO2014075688A1 (ru) |
ZA (1) | ZA201502738B (ru) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105777862A (zh) * | 2012-10-24 | 2016-07-20 | 建新公司 | 使用mes和mops作为流动相改性剂从多峰树脂洗脱生物分子 |
CN108176386B (zh) * | 2018-01-16 | 2020-11-06 | 武汉轻工大学 | 一种三聚氰胺键合色谱固定相及其制备方法和金属离子改性三聚氰胺键合色谱固定相 |
CN113101909B (zh) * | 2021-05-11 | 2023-07-18 | 博格隆(浙江)生物技术有限公司 | 一种层析介质及其制备方法 |
EP4234695A1 (en) * | 2022-02-24 | 2023-08-30 | Sartorius BIA Separations d.o.o. | Methods for the selective removal of contaminants during a process of purifying biologic agents |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK2628746T3 (en) * | 2006-04-04 | 2019-04-01 | Chiesi Farm Spa | A method for the concentration of a polypeptide |
CN101410408A (zh) * | 2006-04-04 | 2009-04-15 | 希尔制药爱尔兰有限责任公司 | 用于浓缩多肽的方法 |
US20080207487A1 (en) * | 2006-11-02 | 2008-08-28 | Neose Technologies, Inc. | Manufacturing process for the production of polypeptides expressed in insect cell-lines |
CA3112632A1 (en) * | 2008-01-18 | 2009-07-23 | Biomarin Pharmaceutical Inc. | Manufacture of active highly phosphorylated human lysosomal sulfatase enzymes and uses thereof |
CN101597614A (zh) * | 2008-11-24 | 2009-12-09 | 中国农业科学院生物技术研究所 | 编码β-半乳糖苷酶的基因及其表达和应用 |
US20110318324A1 (en) * | 2010-06-25 | 2011-12-29 | Shire Human Genetic Therapies, Inc. | Methods and compositions for cns delivery of b-galactocerebrosidase |
-
2013
- 2013-11-13 NZ NZ707753A patent/NZ707753A/en unknown
- 2013-11-13 EP EP13795420.2A patent/EP2920198B1/en active Active
- 2013-11-13 CA CA2890358A patent/CA2890358C/en active Active
- 2013-11-13 PL PL13795420T patent/PL2920198T3/pl unknown
- 2013-11-13 ES ES13795420T patent/ES2751369T3/es active Active
- 2013-11-13 KR KR1020157015728A patent/KR101783466B1/ko active IP Right Grant
- 2013-11-13 EA EA201590778A patent/EA032680B1/ru not_active IP Right Cessation
- 2013-11-13 US US14/437,463 patent/US20150284698A1/en not_active Abandoned
- 2013-11-13 CN CN201380059392.2A patent/CN104854122B/zh active Active
- 2013-11-13 JP JP2015541017A patent/JP6035427B2/ja active Active
- 2013-11-13 BR BR112015010796-6A patent/BR112015010796B1/pt active IP Right Grant
- 2013-11-13 AU AU2013347260A patent/AU2013347260B2/en active Active
- 2013-11-13 WO PCT/DK2013/050378 patent/WO2014075688A1/en active Application Filing
-
2015
- 2015-04-22 ZA ZA2015/02738A patent/ZA201502738B/en unknown
- 2015-05-12 IL IL238775A patent/IL238775B/en active IP Right Grant
-
2016
- 2016-02-18 HK HK16101798.4A patent/HK1213915A1/zh unknown
Also Published As
Publication number | Publication date |
---|---|
AU2013347260B2 (en) | 2016-07-28 |
CA2890358A1 (en) | 2014-05-22 |
IL238775B (en) | 2019-02-28 |
CN104854122A (zh) | 2015-08-19 |
BR112015010796A2 (ru) | 2017-08-22 |
JP6035427B2 (ja) | 2016-11-30 |
EP2920198B1 (en) | 2019-09-25 |
BR112015010796B1 (pt) | 2022-08-02 |
NZ707753A (en) | 2019-07-26 |
ZA201502738B (en) | 2019-09-25 |
EP2920198A1 (en) | 2015-09-23 |
EA032680B1 (ru) | 2019-07-31 |
IL238775A0 (en) | 2015-06-30 |
US20150284698A1 (en) | 2015-10-08 |
KR101783466B1 (ko) | 2017-10-10 |
HK1213915A1 (zh) | 2016-07-15 |
KR20150117638A (ko) | 2015-10-20 |
WO2014075688A1 (en) | 2014-05-22 |
PL2920198T3 (pl) | 2020-03-31 |
CA2890358C (en) | 2020-02-11 |
AU2013347260A1 (en) | 2015-05-21 |
ES2751369T3 (es) | 2020-03-31 |
CN104854122B (zh) | 2021-10-08 |
JP2015535422A (ja) | 2015-12-14 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ BY KZ KG TJ TM |