DK2691411T3 - Buffersystem til proteinoprensning - Google Patents

Buffersystem til proteinoprensning Download PDF

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Publication number
DK2691411T3
DK2691411T3 DK12764025.8T DK12764025T DK2691411T3 DK 2691411 T3 DK2691411 T3 DK 2691411T3 DK 12764025 T DK12764025 T DK 12764025T DK 2691411 T3 DK2691411 T3 DK 2691411T3
Authority
DK
Denmark
Prior art keywords
protein purification
buffer system
purification buffer
protein
purification
Prior art date
Application number
DK12764025.8T
Other languages
English (en)
Inventor
Kent E Goklen
Eric J Suda
Antonio Raul Ubiera
Original Assignee
Glaxosmithkline Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=46931905&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK2691411(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Glaxosmithkline Llc filed Critical Glaxosmithkline Llc
Application granted granted Critical
Publication of DK2691411T3 publication Critical patent/DK2691411T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
DK12764025.8T 2011-03-29 2012-03-29 Buffersystem til proteinoprensning DK2691411T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161468814P 2011-03-29 2011-03-29
PCT/US2012/031076 WO2012135415A1 (en) 2011-03-29 2012-03-29 Buffer system for protein purification

Publications (1)

Publication Number Publication Date
DK2691411T3 true DK2691411T3 (da) 2020-05-11

Family

ID=46931905

Family Applications (1)

Application Number Title Priority Date Filing Date
DK12764025.8T DK2691411T3 (da) 2011-03-29 2012-03-29 Buffersystem til proteinoprensning

Country Status (15)

Country Link
US (2) US9624261B2 (da)
EP (1) EP2691411B1 (da)
JP (1) JP6147726B2 (da)
KR (1) KR102058254B1 (da)
CN (2) CN107915769A (da)
AU (1) AU2012236486B2 (da)
BR (1) BR112013024933A2 (da)
CA (1) CA2831787A1 (da)
DK (1) DK2691411T3 (da)
EA (1) EA028326B1 (da)
ES (1) ES2784628T3 (da)
IL (1) IL228610B (da)
MY (1) MY162570A (da)
SG (1) SG193545A1 (da)
WO (1) WO2012135415A1 (da)

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JP6663360B2 (ja) 2015-01-26 2020-03-11 株式会社カネカ 変異型免疫グロブリンκ鎖可変領域結合性ペプチド
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US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
US10513537B2 (en) 2016-05-11 2019-12-24 Ge Healthcare Bioprocess R&D Ab Separation matrix
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
CN109311948B (zh) 2016-05-11 2022-09-16 思拓凡生物工艺研发有限公司 清洁和/或消毒分离基质的方法
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KR20190047376A (ko) * 2017-10-27 2019-05-08 주식회사 녹십자 개선된 면역글로불린의 정제방법
KR20190092951A (ko) * 2018-01-31 2019-08-08 씨제이제일제당 (주) 연속식 크로마토그래피 공정을 이용한 천연 l-시스테인 결정의 제조 방법
CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
US11612885B2 (en) * 2018-05-08 2023-03-28 Waters Technologies Corporation Methods, compositions and kits useful for pH gradient cation exchange chromatography
CN112566930A (zh) * 2018-06-19 2021-03-26 百时美施贵宝公司 使用色谱纯化蛋白质的方法
RU2693251C1 (ru) * 2018-11-12 2019-07-01 Федеральное государственное бюджетное учреждение науки Институт химической биологии и фундаментальной медицины Сибирского отделения Российской академии наук (ИХБФМ СО РАН) Способ выделения и очистки рекомбинантного белка, аналога фрагмента каппа-казеина человека, обладающего цитотоксической активностью по отношению к раковым клеткам человека
CN110437302A (zh) * 2019-07-19 2019-11-12 上海药明生物技术有限公司 基于商业蛋白层析系统的半自动化高通量纯化方法
WO2022172284A1 (en) * 2021-02-11 2022-08-18 Gennova Biopharmaceuticals Ltd. Purification of a therapeutic protein
CN113980092A (zh) * 2021-12-09 2022-01-28 上海药明生物技术有限公司 一种蛋白质亲和纯化方法
WO2023194837A1 (en) * 2022-04-04 2023-10-12 Intas Pharmaceuticals Ltd. Multi-component buffer system for purification of antibodies

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Also Published As

Publication number Publication date
IL228610A0 (en) 2013-12-31
CN107915769A (zh) 2018-04-17
US20140018525A1 (en) 2014-01-16
EA028326B1 (ru) 2017-11-30
US20170174721A1 (en) 2017-06-22
EA201391402A1 (ru) 2014-01-30
US9868761B2 (en) 2018-01-16
SG193545A1 (en) 2013-11-29
EP2691411B1 (en) 2020-02-26
CN103547589A (zh) 2014-01-29
CA2831787A1 (en) 2012-10-04
KR102058254B1 (ko) 2019-12-20
JP6147726B2 (ja) 2017-06-14
KR20140022849A (ko) 2014-02-25
WO2012135415A1 (en) 2012-10-04
MY162570A (en) 2017-06-30
EP2691411A4 (en) 2014-09-10
ES2784628T3 (es) 2020-09-29
AU2012236486A1 (en) 2013-10-17
IL228610B (en) 2018-06-28
BR112013024933A2 (pt) 2016-09-06
EP2691411A1 (en) 2014-02-05
US9624261B2 (en) 2017-04-18
JP2014509651A (ja) 2014-04-21
AU2012236486B2 (en) 2016-06-23

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