EA201391402A1 - Буферная система для очистки белка - Google Patents

Буферная система для очистки белка

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Publication number
EA201391402A1
EA201391402A1 EA201391402A EA201391402A EA201391402A1 EA 201391402 A1 EA201391402 A1 EA 201391402A1 EA 201391402 A EA201391402 A EA 201391402A EA 201391402 A EA201391402 A EA 201391402A EA 201391402 A1 EA201391402 A1 EA 201391402A1
Authority
EA
Eurasian Patent Office
Prior art keywords
buffer system
acid
component
cleaning protein
protein
Prior art date
Application number
EA201391402A
Other languages
English (en)
Other versions
EA028326B1 (ru
Inventor
Кент И. Гоклин
Эрик Дж. Суда
Антонио Рауль Убиера
Original Assignee
ГЛЭКСОСМИТКЛАЙН ЭлЭлСи
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=46931905&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA201391402(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by ГЛЭКСОСМИТКЛАЙН ЭлЭлСи filed Critical ГЛЭКСОСМИТКЛАЙН ЭлЭлСи
Publication of EA201391402A1 publication Critical patent/EA201391402A1/ru
Publication of EA028326B1 publication Critical patent/EA028326B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Способ очистки белка с использованием упрощенной, не содержащей хлорид натрия буферной системы, которая состоит из двух компонентов (пары кислоты и основания) для подходящего контроля рН раствора и третьего компонента для контроля ионной силы, где третий компонент представляет собой соль натрия и основания, сопряженного с кислотой.
EA201391402A 2011-03-29 2012-03-29 Многокомпонентная буферная система для очистки рекомбинантного белка и способы очистки белка EA028326B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161468814P 2011-03-29 2011-03-29
PCT/US2012/031076 WO2012135415A1 (en) 2011-03-29 2012-03-29 Buffer system for protein purification

Publications (2)

Publication Number Publication Date
EA201391402A1 true EA201391402A1 (ru) 2014-01-30
EA028326B1 EA028326B1 (ru) 2017-11-30

Family

ID=46931905

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201391402A EA028326B1 (ru) 2011-03-29 2012-03-29 Многокомпонентная буферная система для очистки рекомбинантного белка и способы очистки белка

Country Status (15)

Country Link
US (2) US9624261B2 (ru)
EP (1) EP2691411B1 (ru)
JP (1) JP6147726B2 (ru)
KR (1) KR102058254B1 (ru)
CN (2) CN107915769A (ru)
AU (1) AU2012236486B2 (ru)
BR (1) BR112013024933A2 (ru)
CA (1) CA2831787A1 (ru)
DK (1) DK2691411T3 (ru)
EA (1) EA028326B1 (ru)
ES (1) ES2784628T3 (ru)
IL (1) IL228610B (ru)
MY (1) MY162570A (ru)
SG (1) SG193545A1 (ru)
WO (1) WO2012135415A1 (ru)

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EP2994485B1 (en) * 2013-05-06 2018-01-10 Sanofi Continuous multistep process for purifying antibodies
JP6602765B2 (ja) 2013-09-05 2019-11-06 ジェネンテック， インコーポレイテッド クロマトグラフィー再使用のための方法
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JP6663360B2 (ja) 2015-01-26 2020-03-11 株式会社カネカ 変異型免疫グロブリンκ鎖可変領域結合性ペプチド
US11407981B2 (en) 2016-03-11 2022-08-09 Boehringer Ingelheim International Gmbh Methods for continuously inactivating a virus during manufacture of a protein
WO2017195638A1 (ja) 2016-05-09 2017-11-16 株式会社カネカ 抗体またはκ鎖可変領域含有抗体断片の精製方法
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CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
US11612885B2 (en) * 2018-05-08 2023-03-28 Waters Technologies Corporation Methods, compositions and kits useful for pH gradient cation exchange chromatography
CN112566930A (zh) * 2018-06-19 2021-03-26 百时美施贵宝公司 使用色谱纯化蛋白质的方法
RU2693251C1 (ru) * 2018-11-12 2019-07-01 Федеральное государственное бюджетное учреждение науки Институт химической биологии и фундаментальной медицины Сибирского отделения Российской академии наук (ИХБФМ СО РАН) Способ выделения и очистки рекомбинантного белка, аналога фрагмента каппа-казеина человека, обладающего цитотоксической активностью по отношению к раковым клеткам человека
CN110437302A (zh) * 2019-07-19 2019-11-12 上海药明生物技术有限公司 基于商业蛋白层析系统的半自动化高通量纯化方法
WO2022172284A1 (en) * 2021-02-11 2022-08-18 Gennova Biopharmaceuticals Ltd. Purification of a therapeutic protein
CN113980092B (zh) * 2021-12-09 2024-05-14 上海药明生物技术有限公司 一种蛋白质亲和纯化方法
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Also Published As

Publication number Publication date
EA028326B1 (ru) 2017-11-30
IL228610B (en) 2018-06-28
SG193545A1 (en) 2013-11-29
BR112013024933A2 (pt) 2016-09-06
US9624261B2 (en) 2017-04-18
AU2012236486A1 (en) 2013-10-17
KR20140022849A (ko) 2014-02-25
EP2691411A4 (en) 2014-09-10
KR102058254B1 (ko) 2019-12-20
US20140018525A1 (en) 2014-01-16
EP2691411B1 (en) 2020-02-26
CN103547589A (zh) 2014-01-29
ES2784628T3 (es) 2020-09-29
WO2012135415A1 (en) 2012-10-04
US20170174721A1 (en) 2017-06-22
CN107915769A (zh) 2018-04-17
EP2691411A1 (en) 2014-02-05
DK2691411T3 (da) 2020-05-11
JP6147726B2 (ja) 2017-06-14
JP2014509651A (ja) 2014-04-21
CA2831787A1 (en) 2012-10-04
AU2012236486B2 (en) 2016-06-23
US9868761B2 (en) 2018-01-16
MY162570A (en) 2017-06-30
IL228610A0 (en) 2013-12-31

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