MX2015010006A - Purificacion de proteinas mejorada a traves de una etapa de desglicosilacion. - Google Patents

Purificacion de proteinas mejorada a traves de una etapa de desglicosilacion.

Info

Publication number
MX2015010006A
MX2015010006A MX2015010006A MX2015010006A MX2015010006A MX 2015010006 A MX2015010006 A MX 2015010006A MX 2015010006 A MX2015010006 A MX 2015010006A MX 2015010006 A MX2015010006 A MX 2015010006A MX 2015010006 A MX2015010006 A MX 2015010006A
Authority
MX
Mexico
Prior art keywords
proteins via
improved purification
deglycosylation step
deglycosylation
purification
Prior art date
Application number
MX2015010006A
Other languages
English (en)
Inventor
Jonas Jacobsen
Sari Charlotte Hansen
Hans Bisgaard-Frantzen
Johannes Maarten Van Den Brink
Jesper Langholm Jensen
Original Assignee
Chr Hansen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chr Hansen As filed Critical Chr Hansen As
Publication of MX2015010006A publication Critical patent/MX2015010006A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23023—Mucorpepsin (3.4.23.23)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Se describe un método para purificar un polipéptido de interés mediante el uso de una etapa de desglicosilación.
MX2015010006A 2013-02-05 2014-01-31 Purificacion de proteinas mejorada a traves de una etapa de desglicosilacion. MX2015010006A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13154063 2013-02-05
EP13154797 2013-02-11
PCT/EP2014/051931 WO2014122079A2 (en) 2013-02-05 2014-01-31 Improved purification of proteins via a deglycosylation step

Publications (1)

Publication Number Publication Date
MX2015010006A true MX2015010006A (es) 2015-10-12

Family

ID=50029060

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015010006A MX2015010006A (es) 2013-02-05 2014-01-31 Purificacion de proteinas mejorada a traves de una etapa de desglicosilacion.

Country Status (7)

Country Link
US (1) US20160002618A1 (es)
EP (1) EP2953961A2 (es)
JP (1) JP2016504920A (es)
CN (1) CN105121456A (es)
HK (1) HK1211945A1 (es)
MX (1) MX2015010006A (es)
WO (1) WO2014122079A2 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102399639B1 (ko) * 2016-12-02 2022-05-18 다이이찌 산쿄 가부시키가이샤 신규 엔도-β-N-아세틸글루코사미니다아제
US10073464B2 (en) 2016-12-30 2018-09-11 Bendix Commercial Vehicle Systems Llc Varying the distance between vehicles in a platoon
ES2894173A1 (es) * 2020-08-03 2022-02-11 Soria Natural S A Procedimiento para la preparacion de una bebida de soja

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ285373A (en) * 1994-05-03 1997-09-22 Hansens Lab Process of separating milk clotting enzymes such as aspartic endopeptidases, and liquid and powder rennet compositions
WO1996019582A1 (en) * 1994-12-21 1996-06-27 Chr. Hansen A/S Microbially derived rennin having enhanced milk clotting activity and method of producing same
WO2001058924A2 (en) * 2000-02-08 2001-08-16 Upfront Chromatography A/S Novel resin materials and their use for recovering proteins or peptides
AU2002213838A1 (en) * 2000-11-06 2002-05-15 Chr. Hansen A/S Method of producing non-bovine chymosin and use thereof
SE0004808D0 (sv) * 2000-12-20 2000-12-20 Apbiotech Ab Method for the purification of an enzyme
DK1515986T3 (da) * 2002-06-17 2019-01-02 Chr Hansen As Forbedret fremgangsmåde til fremstilling af en asparaginprotease i en rekombinant værtsorganisme
US20070269863A1 (en) * 2005-12-22 2007-11-22 Bridon Dominique P Process for the production of preformed conjugates of albumin and a therapeutic agent
EP3763220A1 (en) * 2007-02-13 2021-01-13 Chr. Hansen A/S Coagulation of milk

Also Published As

Publication number Publication date
US20160002618A1 (en) 2016-01-07
WO2014122079A2 (en) 2014-08-14
JP2016504920A (ja) 2016-02-18
EP2953961A2 (en) 2015-12-16
CN105121456A (zh) 2015-12-02
HK1211945A1 (en) 2016-06-03
WO2014122079A3 (en) 2014-11-06

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