ATE421536T1 - Verfahren zur reinigung stark anionischer proteine - Google Patents

Verfahren zur reinigung stark anionischer proteine

Info

Publication number
ATE421536T1
ATE421536T1 AT06010184T AT06010184T ATE421536T1 AT E421536 T1 ATE421536 T1 AT E421536T1 AT 06010184 T AT06010184 T AT 06010184T AT 06010184 T AT06010184 T AT 06010184T AT E421536 T1 ATE421536 T1 AT E421536T1
Authority
AT
Austria
Prior art keywords
purifying
containing molecules
mixture
anionic proteins
strongly anionic
Prior art date
Application number
AT06010184T
Other languages
English (en)
Inventor
Jonathan L Coffmann
William B Foster
Shujun Sun
Original Assignee
Genetics Inst Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Inst Llc filed Critical Genetics Inst Llc
Application granted granted Critical
Publication of ATE421536T1 publication Critical patent/ATE421536T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/828—Protein A

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT06010184T 2001-06-05 2002-06-05 Verfahren zur reinigung stark anionischer proteine ATE421536T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US29640201P 2001-06-05 2001-06-05

Publications (1)

Publication Number Publication Date
ATE421536T1 true ATE421536T1 (de) 2009-02-15

Family

ID=23141860

Family Applications (2)

Application Number Title Priority Date Filing Date
AT02749564T ATE333469T1 (de) 2001-06-05 2002-06-05 Verfahren zur reinigung hochanionischer proteine
AT06010184T ATE421536T1 (de) 2001-06-05 2002-06-05 Verfahren zur reinigung stark anionischer proteine

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT02749564T ATE333469T1 (de) 2001-06-05 2002-06-05 Verfahren zur reinigung hochanionischer proteine

Country Status (11)

Country Link
US (1) US7223848B2 (de)
EP (2) EP1714982B1 (de)
JP (1) JP4288153B2 (de)
AT (2) ATE333469T1 (de)
AU (1) AU2008203302B2 (de)
CA (1) CA2448788C (de)
DE (2) DE60213248T2 (de)
DK (2) DK1714982T3 (de)
ES (2) ES2266535T3 (de)
NZ (1) NZ530025A (de)
WO (1) WO2002098531A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8084032B2 (en) * 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
EP1698637B1 (de) * 2005-03-03 2008-10-15 Ajinomoto Co., Inc. Methode zur Verbesserung der Ausbeute bei der Proteinreinigung mittels Gelfiltrationschromatographie und Arginin
CA2632519A1 (en) * 2005-12-06 2007-07-05 Amgen Inc. Polishing steps used in multi-step protein purification processes
CA2636109A1 (en) * 2006-01-06 2007-07-19 Amgen Inc. Methods and systems for isolating target molecules from complex solutions by column-chromatography using eluants containing organic solvents
PE20080913A1 (es) * 2006-09-08 2008-08-21 Wyeth Corp Lavado de arginina en la purificacion de proteinas usando cromatografia de afinidad
NZ575328A (en) 2006-09-13 2012-06-29 Abbott Lab Cell culture improvements
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
EP2089429B1 (de) * 2006-10-19 2014-11-26 Janssen Biotech, Inc. Verfahren zur herstellung nicht aggregierter antikörper-fc-domänen
JP5808249B2 (ja) 2008-10-20 2015-11-10 アッヴィ・インコーポレイテッド プロテインａアフィニティークロマトグラフィーを用いる抗体の単離および精製
AU2009307737B2 (en) 2008-10-20 2015-07-23 Abbvie Inc. Viral inactivation during purification of antibodies
WO2010080065A1 (en) * 2009-01-12 2010-07-15 Ge Healthcare Bio-Sciences Ab Affinity chromatography matrix
WO2011064910A1 (ja) * 2009-11-25 2011-06-03 パナソニック株式会社 免疫測定方法
DK2513134T3 (en) 2009-12-18 2017-12-18 Novartis Ag Washing solution and process for affinity chromatography
JP6366502B2 (ja) 2011-06-01 2018-08-01 ノバルティス アーゲー アフィニティクロマトグラフィーのための、洗浄溶液および方法
CN114901671B (zh) * 2020-01-20 2024-02-20 上海药明生物技术有限公司 用于亲和层析的新的清洗缓冲溶液

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4983722A (en) 1988-06-08 1991-01-08 Miles Inc. Removal of protein A from antibody preparations
US5115101A (en) * 1988-06-08 1992-05-19 Miles Inc. Removal of protein A from antibody preparations
US5464778A (en) 1989-03-08 1995-11-07 Board Of Regents Of The University Of Oklahoma Glycoprotein ligand for P-selectin and methods of use thereof
GB9022547D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Purified immunoglobulin
US5726293A (en) * 1992-10-02 1998-03-10 The General Hospital Corporation Affinity purification methods involving imidazole elution
US5843707A (en) 1992-10-23 1998-12-01 Genetics Institute, Inc. Nucleic acid encoding a novel P-selectin ligand protein
US6277975B1 (en) 1992-10-23 2001-08-21 Genetics Institute, Inc. Fusions of P-selectin ligand protein and polynucleotides encoding same
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
ES2161793T3 (es) 1994-04-09 2001-12-16 Hoffmann La Roche Proceso para la produccion de alfa-interferona.
US6008328A (en) 1994-10-13 1999-12-28 Amgen Inc. Method for purifying keratinocyte growth factors
SE503424C2 (sv) 1994-11-14 1996-06-10 Pharmacia Ab Process för rening av rekombinant koagulationsfaktor VIII
FR2737730B1 (fr) 1995-08-10 1997-09-05 Pasteur Merieux Serums Vacc Procede de purification de virus par chromatographie
FR2738009B1 (fr) 1995-08-24 1997-10-31 Centre Nat Rech Scient Procede d'obtention de polysaccharides sulfates
PL191400B1 (pl) 1996-11-15 2006-05-31 Genentech Inc Sposób izolowania neurotrofiny z mieszaniny białek i kompozycja neurotrofiny
PT950067E (pt) 1996-11-27 2007-12-06 Genentech Inc Purificação por afinidade de um polipéptido numa matriz de proteína a.
ES2262340T3 (es) 1998-09-24 2006-11-16 Pharming Intellectual Property Bv Purificacion del fibrinogeno de la leche mediante el uso de cromatografia de intercambio cationico.
IL142717A0 (en) 1998-10-30 2002-03-10 Genetics Inst Inhibition of differentiation of cytotoxic t-cells by p-selectin ligand (psgl) antagonists
CA2359242C (en) 1999-01-21 2009-12-08 Metamorphix, Inc. Growth differentiation factor inhibitors and uses therefor
CA2404155C (en) * 2000-03-27 2011-08-09 Genetics Institute, Llc. Methods for purifying highly anionic proteins

Also Published As

Publication number Publication date
EP1404428A1 (de) 2004-04-07
NZ530025A (en) 2005-06-24
JP4288153B2 (ja) 2009-07-01
AU2008203302B2 (en) 2011-10-27
DE60213248T2 (de) 2006-11-30
ATE333469T1 (de) 2006-08-15
EP1404428B1 (de) 2006-07-19
EP1714982A3 (de) 2006-12-20
ES2321539T3 (es) 2009-06-08
EP1404428A4 (de) 2004-12-08
EP1714982A2 (de) 2006-10-25
DE60213248D1 (de) 2006-08-31
DE60231016D1 (de) 2009-03-12
CA2448788C (en) 2010-08-24
DK1714982T3 (da) 2009-05-04
CA2448788A1 (en) 2002-12-12
ES2266535T3 (es) 2007-03-01
AU2008203302A1 (en) 2008-08-14
JP2004537042A (ja) 2004-12-09
US7223848B2 (en) 2007-05-29
EP1714982B1 (de) 2009-01-21
WO2002098531A1 (en) 2002-12-12
DK1404428T3 (da) 2006-10-30
US20030050450A1 (en) 2003-03-13

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