PE20080913A1 - Lavado de arginina en la purificacion de proteinas usando cromatografia de afinidad - Google Patents

Lavado de arginina en la purificacion de proteinas usando cromatografia de afinidad

Info

Publication number
PE20080913A1
PE20080913A1 PE2007001208A PE2007001208A PE20080913A1 PE 20080913 A1 PE20080913 A1 PE 20080913A1 PE 2007001208 A PE2007001208 A PE 2007001208A PE 2007001208 A PE2007001208 A PE 2007001208A PE 20080913 A1 PE20080913 A1 PE 20080913A1
Authority
PE
Peru
Prior art keywords
product
media
washing
affinity chromatography
protein purification
Prior art date
Application number
PE2007001208A
Other languages
English (en)
Inventor
Shujun Sun
Christopher Gallo
Original Assignee
Wyeth Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=39158086&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PE20080913(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Wyeth Corp filed Critical Wyeth Corp
Publication of PE20080913A1 publication Critical patent/PE20080913A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

REFERIDA A UN METODO PARA AISLAR UN PRODUCTO QUE COMPRENDE: a) SUMINISTRAR UN FLUIDO DE CARGA QUE COMPRENDE UN PRODUCTO Y UNA O MAS IMPUREZAS, b) PONER EN CONTACTO EL FLUIDO DE CARGA CON UN MEDIO QUE PUEDE ENLAZAR EL PRODUCTO BAJO CONDICIONES ADECUADAS PARA ENLAZAR EL PRODUCTO Y SUMINISTRAR UN MEDIO ENLAZADO, c) PONER EN CONTACTO EL MEDIO ENLAZADO CON UNA O MAS SOLUCIONES DE LAVADO A TRAVES DEL MEDIO ENLAZADO, DONDE AL MENOS UNA SOLUCION DE LAVADO COMPRENDE ARGININA EN UNA CONCENTRACION DIFERENTE DE 1M, CON LO QUE SE PROPORCIONA UN MEDIO LAVADO, d) PONER EN CONTACTO EL MEDIO LAVADO CON UNA SOLUCION DE ELUCION BAJO CONDICIONES ADECUADAS Y e) RECOLECTAR UN PRODUCTO DE ELUCION QUE COMPRENDE EL PRODUCTO. EL PRODUCTO ES UN ANTICUERPO ESPECIFICO PARA GDF-8, IL-13, IL-22, RAGE O A-BETA
PE2007001208A 2006-09-08 2007-09-07 Lavado de arginina en la purificacion de proteinas usando cromatografia de afinidad PE20080913A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US84308406P 2006-09-08 2006-09-08

Publications (1)

Publication Number Publication Date
PE20080913A1 true PE20080913A1 (es) 2008-08-21

Family

ID=39158086

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2007001208A PE20080913A1 (es) 2006-09-08 2007-09-07 Lavado de arginina en la purificacion de proteinas usando cromatografia de afinidad

Country Status (17)

Country Link
US (2) US8350013B2 (es)
EP (1) EP2059258B1 (es)
JP (1) JP5386354B2 (es)
KR (1) KR20090064377A (es)
CN (1) CN101511387A (es)
AR (1) AR062711A1 (es)
AU (1) AU2007292253A1 (es)
BR (1) BRPI0716597A2 (es)
CA (1) CA2661645C (es)
CL (1) CL2007002615A1 (es)
ES (1) ES2764222T3 (es)
IL (1) IL197053A0 (es)
MX (1) MX2009002576A (es)
PE (1) PE20080913A1 (es)
RU (1) RU2009106083A (es)
TW (1) TW200821026A (es)
WO (1) WO2008031020A2 (es)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2007272494B2 (en) 2006-07-14 2011-07-28 Wisconsin Alumni Research Foundation Adsorptive membranes for trapping viruses
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
WO2008033517A2 (en) 2006-09-13 2008-03-20 Abbott Laboratories Cell culture improvements
JP5448825B2 (ja) * 2006-10-19 2014-03-19 ヤンセン バイオテツク，インコーポレーテツド 非凝集抗体Ｆｃドメインの製造方法
EP1958626B1 (en) * 2007-02-13 2014-07-23 Ajinomoto Co., Inc. Method for inactivating viruses by addition of slightly acidic arginine
EP2921501A1 (en) * 2008-10-20 2015-09-23 Abbvie Inc. Isolation and purification of antibodies using Protein A affinity chromatography
CN104974251A (zh) 2008-10-20 2015-10-14 Abbvie公司 在抗体纯化过程中的病毒灭活
DK2350271T3 (en) 2008-11-20 2016-04-04 Biogen Ma Inc ARGININ INACTIVATION OF ENVIRONMENT VIRA
EP2406274A2 (en) 2009-03-11 2012-01-18 Wyeth LLC Methods of purifying small modular immunopharmaceutical proteins
SG10201407983VA (en) 2009-12-18 2015-01-29 Csl Ltd Method of purifying polypeptides
WO2011073389A1 (en) * 2009-12-18 2011-06-23 Novartis Ag Wash solution and method for affinity chromatography
DK2526114T3 (da) * 2010-01-22 2014-09-29 Boehringer Ingelheim Int Kromatografisk fremgangsmåde til oprensning af FC-indeholdende proteiner
EP2583973B1 (en) * 2010-06-21 2018-03-21 Kyowa Hakko Kirin Co., Ltd. Method for purifying protein using amino acid
WO2012164046A1 (en) * 2011-06-01 2012-12-06 Novartis Ag Wash solution and method for affinity chromatography
WO2013055958A1 (en) 2011-10-11 2013-04-18 Genentech, Inc. Improved assembly of bispecific antibodies
US20130109807A1 (en) * 2011-10-26 2013-05-02 Bio-Rad Laboratories, Inc. Removal of virucidal agents in mixed mode chromatography
AR096713A1 (es) 2013-06-25 2016-01-27 Cadila Healthcare Ltd Proceso de purificación para anticuerpos monoclonales
KR101414897B1 (ko) * 2013-11-29 2014-07-04 씨제이헬스케어 주식회사 다베포에틴 알파의 정제 방법
TR201904928T4 (tr) 2014-09-24 2019-05-21 Basf Se Tereftalik asit diesterlerin resirküle alkol dehidratasyonu ile üretimi için yöntem.
BR112017005850A2 (pt) 2014-09-24 2018-02-06 Basf Se processo e aparelho para preparação de um diéster tereftálico.
RU2668964C1 (ru) 2014-09-24 2018-10-05 Басф Се Способ получения сложных диэфиров терефталевой кислоты с перекачиванием реакционной смеси
RU2648983C1 (ru) 2014-09-24 2018-03-29 Басф Се Способ получения сложных диэфиров терефталевой кислоты
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
CA2978095C (en) 2015-03-13 2022-02-15 Bristol-Myers Squibb Company Use of alkaline washes during chromatography to remove impurities
HRP20160086A2 (hr) 2016-01-27 2017-08-11 Sveučilište u Zagrebu Eluens za imunoafinitetnu kromatografiju virusa i proteina
DE102016106271B4 (de) 2016-04-06 2018-03-29 Axagarius Gmbh & Co. Kg Aufreinigung von Nukleinsäure aus einer Nukleinsäure und Endotoxin enthaltenden Probe
CN109071613A (zh) 2016-05-11 2018-12-21 通用电气医疗集团生物工艺研发股份公司 分离基质
WO2017194593A1 (en) 2016-05-11 2017-11-16 Ge Healthcare Bioprocess R&D Ab Method of cleaning and/or sanitizing a separation matrix
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
US11708390B2 (en) 2016-05-11 2023-07-25 Cytiva Bioprocess R&D Ab Method of storing a separation matrix
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
EP3487868A4 (en) 2016-07-25 2020-07-29 Cephalon, Inc. WASHING PAD FOR AFFINITY CHROMATOGRAPHY
WO2018047080A1 (en) * 2016-09-07 2018-03-15 Glaxosmithkline Intellectual Property Development Limited Methods for purifying antibodies
CN110267721B (zh) * 2017-01-30 2022-03-01 瑞泽恩制药公司 用于降低色谱中生物负载的组合物和方法
TW202000891A (zh) * 2018-03-07 2020-01-01 英商葛蘭素史克智慧財產發展有限公司 純化抗體之方法
CN111902720A (zh) 2018-03-21 2020-11-06 沃特世科技公司 基于非抗体高亲和力的样品制备、吸附剂、装置和方法
CN108586606A (zh) * 2018-04-24 2018-09-28 上海药明生物技术有限公司 一种用于去除抗体蛋白中内毒素的方法
US20230143163A1 (en) * 2020-04-27 2023-05-11 Alexion Pharmaceuticals, Inc. Protein a chromatography purification of an antibody
JP2023523824A (ja) * 2020-05-01 2023-06-07 カシーヴ バイオサイエンシズ，リミテッド・ライアビリティ・カンパニー アフィニティークロマトグラフィーの改善されたプロセス
CN113980092B (zh) * 2021-12-09 2024-05-14 上海药明生物技术有限公司 一种蛋白质亲和纯化方法

Family Cites Families (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4010148A (en) * 1975-05-12 1977-03-01 Board Of Regents Of The University Of Texas System Purified thymosin and process
IL47645A (en) * 1975-07-04 1980-10-26 Yeda Res & Dev Substantially pure and uniform thymic hormone thf,its preparation and pharmaceutical compositions containing it
JPS6030291B2 (ja) * 1978-03-20 1985-07-16 森永乳業株式会社 人顆粒球の分化増殖を促進するｈｇｉ糖蛋白質、ｈｇｉ糖蛋白質の製造法及びｈｇｉ糖蛋白質を含有する白血球減少症治療剤
US4230697A (en) * 1978-07-03 1980-10-28 Morinaga Milk Industry Co. Ltd. Virus-inactivated HGI-glycoprotein capable of stimulating proliferation and differentiation of human granulocyte, process for preparing same and leukopenia curative containing same
US4624932A (en) * 1978-09-13 1986-11-25 Samuel Bogoch Recognins and their chemoreciprocals
DK151634C (da) * 1978-09-29 1988-06-20 Mitsubishi Chem Ind Fremgangsmaade til rensning af langkaedet acyl-coenzym-a-syntetase
JPS5569595A (en) * 1978-11-20 1980-05-26 Suguru Abe Novel substance having fibrinolytic activity, remedy for thrombotic embolic disease comprising it, and its preparation
CA1172980A (en) * 1979-04-02 1984-08-21 Kenji Takemoto Pentadecapeptide and process for the preparation thereof
DE3034529C2 (de) * 1980-09-12 1982-09-23 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Leukorekrutin: Ein Entzündungsmediatorprotein aus Säugerserum zur Induzierung einer Leukozytosereaktion, Herstellungsverfahren, Gewinnung in molekular einheitlicher, kristallisierbarer und biologisch spezifisch wirkender Form und Leukorekrutin enthaltendes Arzneimittel
DE3110561A1 (de) * 1981-03-18 1982-11-04 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Chemorekrutine der leukozyten und des entszuendungsgewebes: eine neue klasse natuerlicher leukopoetine zurselektiven chemorekrutierung von leukozyten aus dem knochenmark in den blutstrom (leukozytose- und linskverschiebungsreaktion)
DE3110560A1 (de) * 1981-03-18 1982-10-14 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen "angiotropine der leukozyten und des entzuendungsgewebes: eine neue klasse natuerlicher chemotropischer mitogene fuer das richtungswachstum von blutgefaessen und zur neovaskularisierung von geweben"
US4532207A (en) * 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
US4390468A (en) * 1982-08-04 1983-06-28 Maruzen Oil Co., Ltd. Preparation of antitumor agent from shellfish
US4882421A (en) * 1982-09-23 1989-11-21 Alfacell Corporation Pharmaceutical for treating tumors and method for making it
AU1629383A (en) * 1982-10-02 1984-04-05 Amano Seiyaku K.K. Earthworm tissue protease thrombolytic agents
JPS5980613A (ja) * 1982-10-29 1984-05-10 Mitsui Toatsu Chem Inc 新規なプラスミノ−ゲン・アクチベ−タ及びその製造方法
US4880914A (en) * 1982-11-09 1989-11-14 Cornell Research Foundation, Inc. Assay for qualitatively and/or quantitatively measuring hLH or hCG in body fluids and reagents therefor
US4966888A (en) 1985-07-08 1990-10-30 Cornell Research Foundation, Inc. hCG-hLH receptor and hCG-hLH receptor-hCG complex as antigens, antibodies thereto and contraceptive vaccine
US4639513A (en) * 1984-02-02 1987-01-27 Cuno Inc. Intravenously injectable immunoglobulin G (IGG) and method for producing same
GB8314362D0 (en) * 1983-05-24 1983-06-29 Celltech Ltd Polypeptide and protein products
US4456676A (en) * 1983-06-10 1984-06-26 E. I. Du Pont De Nemours And Company Amine stabilizers for wash-off systems
US4444760A (en) * 1983-06-17 1984-04-24 Merck & Co., Inc. Purification and characterization of a protein fibroblast growth factor
JPS6028999A (ja) * 1983-06-30 1985-02-14 Maruho Kk 細胞増殖促進作用を有するたんぱく質、その組成物と製造方法
JPS60184098A (ja) * 1984-03-02 1985-09-19 Suntory Ltd 新規なペプチド及びこれを有効成分とする利尿剤
JPH0794473B2 (ja) * 1984-03-02 1995-10-11 サントリー株式会社 新規なペプチド及びこれを有効成分とする利尿剤
US4670539A (en) * 1984-07-27 1987-06-02 Board Of Regents, The University Of Texas Peptide growth factors derived from estrogen responsive kidney tissue
US5132214A (en) * 1986-04-09 1992-07-21 Monsanto Company Large scale production of plasminogen activator from normal human colon cells
US5030559A (en) * 1986-04-01 1991-07-09 Board Of Regents, The University Of Texas System Methods and compositions for the identification of metastatic human tumors
US5213970A (en) * 1987-01-23 1993-05-25 Board Of Regents, The University Of Texas System Method for obtaining soluble antitumor factor
US5162507A (en) * 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
DE3877529T2 (de) * 1987-10-23 1996-11-07 Centre Regional De Transfusion Herstellung von hoch-gereingtem menschlichen Faktor IX sowie anderem Plasmaproteinkonzentrat und dessen therapeutische Verwendung.
JPH01238534A (ja) 1988-03-17 1989-09-22 Mitsui Toatsu Chem Inc エンドトキシンの除去方法
US5760183A (en) * 1989-02-17 1998-06-02 Association D'aquitaine Pour De Developpment De La Transfusion Sanguine Et Des Recherches Hematologiques Process for the manufacture of very high-purity antithaemophilic factor (FVIIIC), and von Willebrand factor, and pharmaceutical compositions containing same
DE3914354C1 (es) * 1989-04-29 1990-10-31 Schaper & Bruemmer Gmbh & Co Kg, 3320 Salzgitter, De
DK165090D0 (da) * 1990-07-09 1990-07-09 Kem En Tec As Konglomererede partikler
FR2676231A1 (fr) * 1991-05-07 1992-11-13 Aetsrn Concentre de facteur xi de la coagulation sanguine a haute activite specifique, approprie a usage therapeutique et son procede de preparation.
JPH0518950A (ja) 1991-07-09 1993-01-26 Showa Denko Kk アフイニテイクロマトグラフイー用吸着担体
EP0547544B1 (de) * 1991-12-18 1997-03-12 Hoechst Aktiengesellschaft Verfahren zur Gewinnung von insulinhaltigen Lösungen
US5372942A (en) * 1992-02-10 1994-12-13 Coriell Institute For Medical Research Protease K resistant arginine deiminase, its method of preparation and its use as an anti-neoplastic agent
CH685498A5 (fr) * 1992-02-14 1995-07-31 Om Lab Sa Extrait de macromolécules bactériennes, procédé pour sa préparation et composition pharmaceutique contenant cet extrait.
JP2767521B2 (ja) * 1992-09-04 1998-06-18 農林水産省食品総合研究所長 新規抗腫瘍性蛋白質とその製造法および該蛋白質を有効成分として含有する抗腫瘍剤
AU693436B2 (en) * 1993-03-09 1998-07-02 Genzyme Corporation Isolation of components of interest from milk
US5710126A (en) * 1993-03-12 1998-01-20 Immulogic Pharmaceutical Corporation T cell epitopes of ryegrass pollen allergen
US6607884B1 (en) * 1993-03-19 2003-08-19 The Johns Hopkins University School Of Medicine Methods of detecting growth differentiation factor-8
US5332503A (en) * 1993-04-16 1994-07-26 Baxter International Inc. Process for purifying collagenase
US5330972A (en) * 1993-05-28 1994-07-19 Abbott Laboratories Method of impeding apoptosis of CD4 cells in persons infected with human immunodeficiency virus
JP2971293B2 (ja) * 1993-06-11 1999-11-02 株式会社細菌化学研究所 溶血性連鎖球菌の産生する生理活性物質及びその製造法
US5500413A (en) * 1993-06-29 1996-03-19 Ferring Ab Process for manufacture of 1-deamino-8-D-arginine vasopressin
US5478924A (en) * 1994-02-16 1995-12-26 Cramer; Steven M. Displacement chromatography of proteins using low molecular weight displacers
US5570389A (en) * 1994-06-30 1996-10-29 Compaq Computer Corporation Method for reliable exchange of modem handshaking information over a cellular radio carrier
US5659017A (en) * 1995-11-07 1997-08-19 Alpha Therapeutic Corporation Anion exchange process for the purification of Factor VIII
DE19600939C1 (de) * 1996-01-12 1997-08-28 Immuno Ag Verfahren zur Trennung von IgG und IgA
SE9603068D0 (sv) * 1996-08-23 1996-08-23 Pharmacia & Upjohn Ab Process for purifying a protein
PT950067E (pt) * 1996-11-27 2007-12-06 Genentech Inc Purificação por afinidade de um polipéptido numa matriz de proteína a.
ZA9711580B (en) * 1996-12-25 1999-09-23 Hoechst Marion Roussel Ltd Process for the production of purified dimeric bone morphogenetic factors.
GB9717953D0 (en) * 1997-08-22 1997-10-29 Smithkline Beecham Biolog Vaccine
US6451260B1 (en) * 1997-08-26 2002-09-17 Dyax Corp. Method for producing microporous elements, the microporous elements thus produced and uses thereof
US20030036629A1 (en) * 1997-12-12 2003-02-20 Barry Foster Novel tgf-beta protein purification methods
US6673629B2 (en) * 1998-01-15 2004-01-06 Abbott Laboratories Neutralization of polycations in a chromatographic device for whole blood use
DE19937219A1 (de) * 1999-08-06 2001-02-08 Aventis Behring Gmbh Verfahren zur Reindarstellung der den Blutgerinnungsfaktor VII aktivierenden Protease, ihres Proenzyms oder eines Gemisches beider Proteine mittels Ionenaustauscherchromatographie
WO1999061464A1 (en) * 1998-05-28 1999-12-02 Korea Green Cross Corporation Process of purifying angiogenesis inhibitors
DK2272870T3 (da) * 1998-06-09 2013-08-05 Csl Behring Ag Fremgangsmåde til fremstilling af immunoglobuliner med henblik på intravenøs indgivelse og andre immunoglobulin-lignende produkter.
GB9815666D0 (en) * 1998-07-17 1998-09-16 Smithkline Beecham Plc Novel compounds
RU2144082C1 (ru) 1998-07-23 2000-01-10 Навашин Петр Сергеевич Рекомбинантная плазмида, кодирующая гибридный белок-предшественник инсулина человека (варианты), штамм бактерий e.coli - продуцент гибридного белка-предшественника инсулина человека (варианты) и способ получения инсулина человека
RU2143492C1 (ru) 1998-07-23 1999-12-27 Навашин Петр Сергеевич Рекомбинантная плазмида, кодирующая гибридный белок - предшественник инсулина человека (варианты), штамм бактерий e.coli - продуцент гибридного белка - предшественника инсулина человека (варианты), способ получения инсулина человека
US7198896B2 (en) * 1998-09-04 2007-04-03 Cell Signaling Technology, Inc. Immunoaffinity isolation of modified peptides from complex mixtures
US7300753B2 (en) * 1998-09-04 2007-11-27 John Rush Immunoaffinity isolation of modified peptides from complex mixtures
US6939545B2 (en) * 1999-04-28 2005-09-06 Genetics Institute, Llc Composition and method for treating inflammatory disorders
US7307161B1 (en) * 1999-04-28 2007-12-11 Genetics Institute, Llc Human Gil-19/AE289 polynucleotides
DE19937218A1 (de) * 1999-08-06 2001-02-08 Aventis Behring Gmbh Verfahren zur Reindarstellung der den Blutgerinnungsfaktor VII aktivierenden Protease, ihres Proenzyms oder eines Gemisches beider Proteine mittels Affinitätschromatographie
US6774102B1 (en) * 1999-09-29 2004-08-10 Gambro Dialysatoren Gmbh & Co. Kg Extracorporeal endotoxin removal method
NZ518187A (en) * 1999-11-04 2002-12-20 Genentech Inc Reversed-phase HPLC assay for plasminogen activators
US6825164B1 (en) * 2000-08-14 2004-11-30 The Trustees Of Columbia University In The City Of New York Method to increase cerebral blood flow in amyloid angiopathy
DE10046960A1 (de) * 2000-09-22 2002-04-11 Roche Diagnostics Gmbh Verfahren zur Herstellung einer aktiven, heterodimeren AMW-RT in prokaryotischen Zellen
US20030133939A1 (en) * 2001-01-17 2003-07-17 Genecraft, Inc. Binding domain-immunoglobulin fusion proteins
US7829084B2 (en) * 2001-01-17 2010-11-09 Trubion Pharmaceuticals, Inc. Binding constructs and methods for use thereof
US7754208B2 (en) * 2001-01-17 2010-07-13 Trubion Pharmaceuticals, Inc. Binding domain-immunoglobulin fusion proteins
US7638604B2 (en) * 2001-02-23 2009-12-29 Genetics Institute, Llc Monoclonal antibodies against interleukin-22
US20040058445A1 (en) * 2001-04-26 2004-03-25 Ledbetter Jeffrey Alan Activation of tumor-reactive lymphocytes via antibodies or genes recognizing CD3 or 4-1BB
NZ530025A (en) * 2001-06-05 2005-06-24 Inst Genetics Llc Methods for purifying highly anionic proteins
US20040203115A1 (en) * 2001-10-09 2004-10-14 Giardina Steven L Methods and compositions for production and purification of recombinant staphylococcal enterotoxin b (rseb)
US20040161776A1 (en) * 2001-10-23 2004-08-19 Maddon Paul J. PSMA formulations and uses thereof
WO2003066669A2 (en) * 2002-02-04 2003-08-14 Millipore Corporation Process for removing protein aggregates and virus from a protein solution
US6870034B2 (en) * 2002-02-05 2005-03-22 Genentech, Inc. Protein purification
WO2004000220A2 (en) * 2002-06-21 2003-12-31 Genetix Pharmaceuticals, Inc. Methods for purifying viral particles for gene therapy
US7261893B2 (en) * 2002-10-22 2007-08-28 Wyeth Neutralizing antibodies against GDF-8 and uses therefor
CN1898266B (zh) * 2003-10-27 2011-09-21 惠氏公司 使用羟磷灰石层析除去高分子量聚集体
US7065110B2 (en) * 2004-01-20 2006-06-20 Northrop Grumman Corporation Scalable wavelength shifted laser source and method
US8084032B2 (en) * 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
US7501121B2 (en) * 2004-06-17 2009-03-10 Wyeth IL-13 binding agents
AR049390A1 (es) 2004-06-09 2006-07-26 Wyeth Corp Anticuerpos contra la interleuquina-13 humana y usos de los mismos
CN104610422A (zh) * 2005-03-11 2015-05-13 惠氏公司 弱分配层析的方法

Also Published As

Publication number Publication date
US8350013B2 (en) 2013-01-08
EP2059258A2 (en) 2009-05-20
CA2661645C (en) 2016-11-08
US20080064860A1 (en) 2008-03-13
KR20090064377A (ko) 2009-06-18
CA2661645A1 (en) 2008-03-13
ES2764222T3 (es) 2020-06-02
IL197053A0 (en) 2009-11-18
JP5386354B2 (ja) 2014-01-15
TW200821026A (en) 2008-05-16
JP2010502737A (ja) 2010-01-28
MX2009002576A (es) 2009-03-20
RU2009106083A (ru) 2010-10-20
CL2007002615A1 (es) 2008-04-18
AR062711A1 (es) 2008-11-26
US20080064861A1 (en) 2008-03-13
CN101511387A (zh) 2009-08-19
AU2007292253A1 (en) 2008-03-13
US7714111B2 (en) 2010-05-11
BRPI0716597A2 (pt) 2013-10-08
WO2008031020A3 (en) 2008-10-23
EP2059258A4 (en) 2012-05-02
WO2008031020A2 (en) 2008-03-13
EP2059258B1 (en) 2019-11-13

Similar Documents

Publication Publication Date Title
PE20080913A1 (es) Lavado de arginina en la purificacion de proteinas usando cromatografia de afinidad
AR062570A1 (es) Proceso para la purificacion de las proteinas que contienen fc
CY1116129T1 (el) Καθαρισμος αντισωματος με κατιονανταλλακτικη χρωματογραφια
MY173921A (en) A biosensor and preparation method thereof
AR074015A1 (es) Purificacion de proteinas acidas utilizando cromatografia de hidroxiapatita ceramica
AR098126A1 (es) Anticuerpos anti-ly6e (locus e del complejo del antígeno 6 linfocítico), inmunoconjugados y métodos para usarlos
SG10201908027QA (en) Use of alkaline washes during chromatography to remove impurities
CL2011002269A1 (es) Aparato detector de ciclo de enjuague que contiene: un sensor de conductividad (310) que mide la conductividad del fluido a medida que el flujo sale de un dispositivo que se esta lavando, un transmisor (320) acoplado al sensor de conductividad (310); y metodo asociado (divisional de la solicitud 2990-08).
AR088108A1 (es) METODO PARA DETECTAR ANTICUERPOS ESPECIFICOS Ab EN UNA MUESTRA BIOLOGICA
SG171630A1 (en) Protein detection reagents and methods with dyes and dextrins
MX2016017115A (es) Metodos y reactivos para la purificacion de proteinas.
CL2012001353A1 (es) Método de detección de un evento de soja en una muestra basada en poner en contacto dicha muestra con un polinucleótido específico para el evento aad-12 pdab4468-0416; método para determinar cigocidad de dicho evento en una planta de soja y kit de detección de adn.
DK2118666T3 (da) Fremgangsmåde til normalisering af koncentrationen af analytter i en urinprøve
ZA200800253B (en) Method for the quantitative determination of poloxamers
AR091836A1 (es) Metodo para purificar el factor transgenico vii
HRP20160554T1 (hr) Analitički postupak za molekule fab i fab'
FR2968767B1 (fr) Procede et coffret pour le diagnostic in vitro du cancer de la prostate
GR1007341B (el) Διαγνωστικος προσδιορισμος
EA200801195A1 (ru) Способ концентрирования, очистки и удаления прионового белка
RU2008135056A (ru) Способ обработки поверхности стекловолокнонаполненного политетрафторэтилена
SI1991875T1 (sl) Postopek za dokazovanje patoloških prionov
ATE426611T1 (de) Verfahren zur reinigung eines kalziumionen- bindenden proteins
DE602005027495D1 (de) Verfahren zum nachweis einer an einen nukleären rezeptor gebundenen substanz
TH104746B (th) การทำให้บริสุทธิ์ของแอนตี้บอดี้โดยการโครมาโตกราฟีแบบแลกเปลี่ยนประจุบวก
TH104746A (th) การทำให้บริสุทธิ์ของแอนตี้บอดี้โดยการโครมาโตกราฟีแบบแลกเปลี่ยนประจุบวก

Legal Events

Date Code Title Description
FX Voluntary withdrawal