MX2016017115A - Metodos y reactivos para la purificacion de proteinas. - Google Patents

Metodos y reactivos para la purificacion de proteinas.

Info

Publication number
MX2016017115A
MX2016017115A MX2016017115A MX2016017115A MX2016017115A MX 2016017115 A MX2016017115 A MX 2016017115A MX 2016017115 A MX2016017115 A MX 2016017115A MX 2016017115 A MX2016017115 A MX 2016017115A MX 2016017115 A MX2016017115 A MX 2016017115A
Authority
MX
Mexico
Prior art keywords
methods
buffer
protein
purification
column
Prior art date
Application number
MX2016017115A
Other languages
English (en)
Inventor
Scott Blank Gregory
Liang LIN Jiun-
Original Assignee
Jhl Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jhl Biotech Inc filed Critical Jhl Biotech Inc
Publication of MX2016017115A publication Critical patent/MX2016017115A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente divulgación proporciona composiciones y métodos para purificar una proteína tal como una desoxirribonucleasa utilizando un procedimiento cromatográfico; los métodos incluyen un solo paso cromatográfico y el uso de reguladores de pH salinos de alta concentración; la presente divulgación proporciona métodos para la purificación de una proteína a partir de una muestra que comprenden cargar la muestra en una columna cromatográfica y lavar la columna con por lo menos un regulador de pH que tiene una conductividad muy alta; en algunas modalidades, el regulador de pH de lavado tiene una conductividad de aproximadamente 50 mS/cm, aproximadamente 60 mS/cm, aproximadamente 70 mS/cm, aproximadamente 80 mS/cm, o mayor; en modalidades adicionales, la proteína se eluye de la columna con un regulador de pH de elución después del lavado con el regulador de pH de de conductividad muy alta.
MX2016017115A 2014-06-25 2015-06-25 Metodos y reactivos para la purificacion de proteinas. MX2016017115A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462017153P 2014-06-25 2014-06-25
PCT/US2015/037705 WO2015200645A1 (en) 2014-06-25 2015-06-25 Methods and reagents for purification of proteins

Publications (1)

Publication Number Publication Date
MX2016017115A true MX2016017115A (es) 2018-01-12

Family

ID=54938806

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016017115A MX2016017115A (es) 2014-06-25 2015-06-25 Metodos y reactivos para la purificacion de proteinas.

Country Status (13)

Country Link
US (2) US10370408B2 (es)
EP (1) EP3160611A4 (es)
JP (1) JP2017519838A (es)
KR (1) KR20170026521A (es)
CN (1) CN106794392A (es)
AU (1) AU2015279831A1 (es)
CA (1) CA2951408A1 (es)
IL (1) IL249729A0 (es)
MX (1) MX2016017115A (es)
RU (1) RU2017101727A (es)
SG (1) SG11201610431WA (es)
TW (1) TW201617360A (es)
WO (1) WO2015200645A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170026521A (ko) * 2014-06-25 2017-03-08 제이에이치엘 바이오테크 인코포레이티드 단백질의 정제를 위한 방법 및 시약
US11174284B2 (en) * 2017-04-04 2021-11-16 Scarab Genomics, Llc Purification of CRM 197 from bacteria
NL2019043B1 (en) 2017-04-25 2018-11-05 Illumina Inc Sensors having integrated protection circuitry
WO2019051252A1 (en) 2017-09-07 2019-03-14 Bristol-Myers Squibb Company METHOD FOR DETERMINING PURITY BY CAPILLARY ELECTROPHORESIS
WO2019192877A1 (en) * 2018-04-03 2019-10-10 Merck Patent Gmbh Cex chromatography media and low salt elution of target proteins from biopharmaceutical feeds
EP3810650A1 (en) * 2018-06-19 2021-04-28 Bristol-Myers Squibb Company Methods of purifying proteins using chromatography
CN111346622B (zh) * 2018-12-24 2023-05-09 内蒙古蒙牛乳业（集团）股份有限公司 色谱填料及其制备方法和用途
CN109847406B (zh) * 2019-03-06 2021-03-30 润方（北京）生物医药研究院有限公司 一种用于纯化血红蛋白的阴离子交换层析柱的清洗方法
GB201909081D0 (en) * 2019-06-25 2019-08-07 Psioxus Therapeutics Ltd Method

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1100308A1 (ru) * 1982-12-14 1984-06-30 Тихоокеанский институт биоорганической химии Дальневосточного научного центра АН СССР Способ получени кислой дезоксирибонуклеазы
US5279823A (en) * 1992-06-08 1994-01-18 Genentech, Inc. Purified forms of DNASE
ES2292682T3 (es) * 1998-05-06 2008-03-16 Genentech, Inc. Purificacion de anticuerpos mediante cromatografia de intercambio ionico.
FI120045B (fi) 2005-12-22 2009-06-15 Roal Oy Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit
EP2089524B1 (en) * 2006-11-30 2012-10-10 Novozymes A/S Dnase expression in recombinant host cells
JP2010516651A (ja) 2007-01-17 2010-05-20 メルク セローノ ソシエテ アノニム Ｆｃ含有タンパク質の精製のための方法
EP2125866B1 (en) * 2007-02-28 2013-05-22 Baxter International Inc. Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules
ES2364118T3 (es) * 2007-07-11 2011-08-25 Novo Nordisk A/S Purificación de factor viii usando una resina de modo mixto o multimodal.
EP2027875A1 (en) * 2007-08-23 2009-02-25 Octapharma AG A Process for Isolation and Purification of a Target Protein free of Prion Protein (PrPSC)
US9809639B2 (en) * 2011-06-08 2017-11-07 Agency For Science, Technology And Research Purification of biological products by constrained cohydration chromatography
EP2578286A1 (en) * 2011-10-04 2013-04-10 Merck Patent GmbH Method and apparatus for chromatographic purification
MX2014005330A (es) * 2011-11-02 2014-09-08 Genentech Inc Cromatografia de sobrecarga y elucion.
EP2656892A1 (en) * 2012-04-23 2013-10-30 Merck Patent GmbH Chromatography method
KR20170026521A (ko) 2014-06-25 2017-03-08 제이에이치엘 바이오테크 인코포레이티드 단백질의 정제를 위한 방법 및 시약

Also Published As

Publication number Publication date
EP3160611A4 (en) 2018-02-28
EP3160611A1 (en) 2017-05-03
US20200017545A1 (en) 2020-01-16
KR20170026521A (ko) 2017-03-08
JP2017519838A (ja) 2017-07-20
RU2017101727A (ru) 2018-07-25
CA2951408A1 (en) 2015-12-30
IL249729A0 (en) 2017-02-28
SG11201610431WA (en) 2017-01-27
WO2015200645A1 (en) 2015-12-30
CN106794392A (zh) 2017-05-31
TW201617360A (zh) 2016-05-16
AU2015279831A1 (en) 2017-01-12
US20170198008A1 (en) 2017-07-13
US10370408B2 (en) 2019-08-06

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