SG11201610431WA - Methods and reagents for purification of proteins - Google Patents

Methods and reagents for purification of proteins

Info

Publication number
SG11201610431WA
SG11201610431WA SG11201610431WA SG11201610431WA SG11201610431WA SG 11201610431W A SG11201610431W A SG 11201610431WA SG 11201610431W A SG11201610431W A SG 11201610431WA SG 11201610431W A SG11201610431W A SG 11201610431WA SG 11201610431W A SG11201610431W A SG 11201610431WA
Authority
SG
Singapore
Prior art keywords
reagents
proteins
purification
methods
Prior art date
Application number
SG11201610431WA
Inventor
Jiun-Liang Lin
Gregory Scott Blank
Original Assignee
Jhl Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jhl Biotech Inc filed Critical Jhl Biotech Inc
Publication of SG11201610431WA publication Critical patent/SG11201610431WA/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11201610431WA 2014-06-25 2015-06-25 Methods and reagents for purification of proteins SG11201610431WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462017153P 2014-06-25 2014-06-25
PCT/US2015/037705 WO2015200645A1 (en) 2014-06-25 2015-06-25 Methods and reagents for purification of proteins

Publications (1)

Publication Number Publication Date
SG11201610431WA true SG11201610431WA (en) 2017-01-27

Family

ID=54938806

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201610431WA SG11201610431WA (en) 2014-06-25 2015-06-25 Methods and reagents for purification of proteins

Country Status (13)

Country Link
US (2) US10370408B2 (en)
EP (1) EP3160611A4 (en)
JP (1) JP2017519838A (en)
KR (1) KR20170026521A (en)
CN (1) CN106794392A (en)
AU (1) AU2015279831A1 (en)
CA (1) CA2951408A1 (en)
IL (1) IL249729A0 (en)
MX (1) MX2016017115A (en)
RU (1) RU2017101727A (en)
SG (1) SG11201610431WA (en)
TW (1) TW201617360A (en)
WO (1) WO2015200645A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2016017115A (en) 2014-06-25 2018-01-12 Jhl Biotech Inc Methods and reagents for purification of proteins.
WO2018187388A1 (en) * 2017-04-04 2018-10-11 Scarab Genomics, Llc Improved purification of crm 197 from bacteria
NL2019043B1 (en) * 2017-04-25 2018-11-05 Illumina Inc Sensors having integrated protection circuitry
CN111316091A (en) * 2017-09-07 2020-06-19 百时美施贵宝公司 Method for determining purity by capillary electrophoresis
JP2021519339A (en) * 2018-04-03 2021-08-10 メルク パテント ゲーエムベーハー Low-salt elution of target protein from CEX chromatography medium and biopharmaceutical feedstock
EP3810650A1 (en) * 2018-06-19 2021-04-28 Bristol-Myers Squibb Company Methods of purifying proteins using chromatography
CN111346622B (en) * 2018-12-24 2023-05-09 内蒙古蒙牛乳业（集团）股份有限公司 Chromatographic packing and preparation method and application thereof
CN109847406B (en) * 2019-03-06 2021-03-30 润方（北京）生物医药研究院有限公司 Method for cleaning anion exchange chromatography column for purifying hemoglobin

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1100308A1 (en) * 1982-12-14 1984-06-30 Тихоокеанский институт биоорганической химии Дальневосточного научного центра АН СССР Method for preparing acid desoxyribonuclease
US5279823A (en) 1992-06-08 1994-01-18 Genentech, Inc. Purified forms of DNASE
US6489447B1 (en) * 1998-05-06 2002-12-03 Genentech, Inc. Protein purification
FI120045B (en) 2005-12-22 2009-06-15 Roal Oy Treatment of cellulose materials and enzymes useful therein
DK2431470T3 (en) * 2006-11-30 2018-01-29 Novozymes As DNase expression in recombinant host cells
US8168185B2 (en) 2007-01-17 2012-05-01 Merck Serono Sa Process for the purification of anti CD-25 antibodies
WO2008104372A1 (en) * 2007-02-28 2008-09-04 Baxter International Inc. Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules
AU2008274195B2 (en) * 2007-07-11 2013-09-05 Novo Nordisk A/S Purification of factor VIII using a mixed-mode or multimodal resin
EP2027875A1 (en) * 2007-08-23 2009-02-25 Octapharma AG A Process for Isolation and Purification of a Target Protein free of Prion Protein (PrPSC)
CN103717284B (en) * 2011-06-08 2016-02-24 新加坡科技研究局 By retraining common hydration chromatogram purification biological products
EP2578286A1 (en) * 2011-10-04 2013-04-10 Merck Patent GmbH Method and apparatus for chromatographic purification
ES2717536T3 (en) * 2011-11-02 2019-06-21 Hoffmann La Roche Chromatography of overload and elution
EP2656892A1 (en) * 2012-04-23 2013-10-30 Merck Patent GmbH Chromatography method
MX2016017115A (en) * 2014-06-25 2018-01-12 Jhl Biotech Inc Methods and reagents for purification of proteins.

Also Published As

Publication number Publication date
RU2017101727A (en) 2018-07-25
US10370408B2 (en) 2019-08-06
AU2015279831A1 (en) 2017-01-12
CA2951408A1 (en) 2015-12-30
IL249729A0 (en) 2017-02-28
EP3160611A4 (en) 2018-02-28
CN106794392A (en) 2017-05-31
MX2016017115A (en) 2018-01-12
EP3160611A1 (en) 2017-05-03
TW201617360A (en) 2016-05-16
US20200017545A1 (en) 2020-01-16
WO2015200645A1 (en) 2015-12-30
JP2017519838A (en) 2017-07-20
KR20170026521A (en) 2017-03-08
US20170198008A1 (en) 2017-07-13

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