ATE426611T1 - Verfahren zur reinigung eines kalziumionen- bindenden proteins - Google Patents

Verfahren zur reinigung eines kalziumionen- bindenden proteins

Info

Publication number
ATE426611T1
ATE426611T1 AT01951897T AT01951897T ATE426611T1 AT E426611 T1 ATE426611 T1 AT E426611T1 AT 01951897 T AT01951897 T AT 01951897T AT 01951897 T AT01951897 T AT 01951897T AT E426611 T1 ATE426611 T1 AT E426611T1
Authority
AT
Austria
Prior art keywords
calcium ion
binding protein
purifying
protein
present
Prior art date
Application number
AT01951897T
Other languages
English (en)
Inventor
Hiroshi Mizokami
Shinichi Furukawa
Keishin Sugawara
Tatsufumi Onchi
Kazuhiro Komatsu
Satoshi Koyanagi
Hideo Yoshizaki
Original Assignee
Chemo Sero Therapeut Res Inst
Kowa Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chemo Sero Therapeut Res Inst, Kowa Co filed Critical Chemo Sero Therapeut Res Inst
Application granted granted Critical
Publication of ATE426611T1 publication Critical patent/ATE426611T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6432—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4728—Calcium binding proteins, e.g. calmodulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21006—Coagulation factor Xa (3.4.21.6)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Removal Of Specific Substances (AREA)
AT01951897T 2000-07-21 2001-07-18 Verfahren zur reinigung eines kalziumionen- bindenden proteins ATE426611T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2000220600 2000-07-21

Publications (1)

Publication Number Publication Date
ATE426611T1 true ATE426611T1 (de) 2009-04-15

Family

ID=18715177

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01951897T ATE426611T1 (de) 2000-07-21 2001-07-18 Verfahren zur reinigung eines kalziumionen- bindenden proteins

Country Status (9)

Country Link
US (2) US6869934B2 (de)
EP (1) EP1306383B1 (de)
KR (1) KR100841599B1 (de)
CN (1) CN1250567C (de)
AT (1) ATE426611T1 (de)
AU (1) AU2001272736A1 (de)
CA (1) CA2385371A1 (de)
DE (1) DE60138111D1 (de)
WO (1) WO2002008249A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ603289A (en) 2010-04-29 2014-07-25 Baxter Int Purification method for divalent cation binding proteins on anion exchange resin
CN102382168A (zh) * 2010-09-03 2012-03-21 中国科学院过程工程研究所 一种高效纯化金属离子结合蛋白的离子交换层析分离纯化方法
LT2748179T (lt) 2011-10-14 2022-04-11 Takeda Pharmaceutical Company Limited Baltymų gryninimas anijonų mainų chromatografija
WO2013053888A1 (en) * 2011-10-14 2013-04-18 Baxter International Inc. Protein purification by anion exchange chromatography
DK2970376T3 (en) 2013-03-15 2018-07-02 Baxalta Inc PURIFICATION PROCEDURE FOR VITAMIN K-DEPENDENT PROTEINS BY ANION EXCHANGE CHROMATOGRAPHY
JP6910033B2 (ja) * 2016-08-01 2021-07-28 株式会社カネカ カルシプロテインパーティクルの吸着材、および吸着除去システムとその利用方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1265446A (en) * 1985-09-30 1990-02-06 Masahiro Maki Anticoagulating substance, process for preparing same and anticoagulant comprising same as an effective component
JP2660514B2 (ja) * 1987-02-20 1997-10-08 興和株式会社 抗血液凝固作用を有するポリペプチド
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
DE4021979A1 (de) * 1989-07-15 1991-01-31 Boehringer Ingelheim Int Verfahren zur reinigung von annexinen
JP2743207B2 (ja) 1990-01-25 1998-04-22 財団法人化学及血清療法研究所 Ｃｐｂ―ｉの製造方法並びにこれに用いる組換えプラスミドおよび形質転換酵母
DE4406515C1 (de) * 1994-02-28 1995-10-19 Immuno Ag Verfahren zur Isolierung und Reinigung Vitamin K-abhängiger Proteine
WO1996003655A1 (en) * 1994-07-23 1996-02-08 Boehringer Mannheim Gmbh Method for the determination of the prethrombotic state
US5976832A (en) * 1995-03-06 1999-11-02 Tonen Corporation DNA encoding novel calcium-binding proteins
EP0731166A3 (de) * 1995-03-06 1997-12-29 Tonen Corporation Neue Calcium-bindende Proteine
US5658877A (en) * 1995-05-18 1997-08-19 Wisconsin Alumni Research Foundation Method to treat endotoxin effects by administration of 33 kilodalton phospholipid binding protein
US6645465B2 (en) * 1999-08-06 2003-11-11 Michigan, University Of The Regents Annexin proteins and autoantibodies as serum markers for cancer

Also Published As

Publication number Publication date
KR100841599B1 (ko) 2008-06-26
US6869934B2 (en) 2005-03-22
US20020169294A1 (en) 2002-11-14
KR20020057960A (ko) 2002-07-12
DE60138111D1 (de) 2009-05-07
EP1306383A1 (de) 2003-05-02
AU2001272736A1 (en) 2002-02-05
CN1250567C (zh) 2006-04-12
EP1306383B1 (de) 2009-03-25
WO2002008249A1 (fr) 2002-01-31
EP1306383A4 (de) 2005-01-26
CA2385371A1 (en) 2002-01-31
CN1392878A (zh) 2003-01-22
US20050059805A1 (en) 2005-03-17

Similar Documents

Publication Publication Date Title
Andersson et al. Isolation of phosphoproteins by immobilized metal (Fe3+) affinity chromatography
ATE370961T1 (de) Reinigung von antikörpern durch ionenaustauschchromatographie
ATE462716T1 (de) Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen
EA201500952A1 (ru) Способы очистки рекомбинантного adamts13 и других белков и их композиции
ATE337058T1 (de) Kolonne und mit dieser durchgeführtes chromatographisches verfahren
WO2004024866A3 (en) Protein purification
PL374311A1 (en) Methods for purifying protein
ATE525087T1 (de) Verfahren zur aufreinigung von antikörpern
PH12013501128A1 (en) Antibody purification by cation exchange chromatography
KR900006511A (ko) 단백질 정제방법
AR062570A1 (es) Proceso para la purificacion de las proteinas que contienen fc
DE502005007091D1 (de) Verfahren zur reinigung von erythropoietin
WO2007109163A3 (en) Wash buffer and method of using
MX2016017115A (es) Metodos y reactivos para la purificacion de proteinas.
ATE426611T1 (de) Verfahren zur reinigung eines kalziumionen- bindenden proteins
ATE328007T1 (de) Reinigung von fibrinogen aus flüssigkeiten mittels ausfällung und hydrophobischer- interaktion-chromatographie
DE602004024298D1 (de) Magnetische moleküle: funktionalisierte magnetische ferritine verwendendes verfahren zur selektiven beseitigung von verunreinigungen aus lösungen durch magnetfiltration
GB2385591B (en) Method of purifying nucleic acids using an aromatic thioether ligands
ATE454471T1 (de) Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren
EA200801195A1 (ru) Способ концентрирования, очистки и удаления прионового белка
RU2008107317A (ru) Метод хроматографии сродства к антитромбину iii
ME01043B (me) Metode za prećišćavanje fsh-a
DE50106666D1 (de) Verfahren zur Vorbehandlung von organisch belastetem Wasser
TH104746B (th) การทำให้บริสุทธิ์ของแอนตี้บอดี้โดยการโครมาโตกราฟีแบบแลกเปลี่ยนประจุบวก
ATE241636T1 (de) Verfahren zur herstellung von einem holzkohl-gm1- komplex

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties