ATE525087T1 - Verfahren zur aufreinigung von antikörpern - Google Patents

Verfahren zur aufreinigung von antikörpern

Info

Publication number
ATE525087T1
ATE525087T1 AT06753791T AT06753791T ATE525087T1 AT E525087 T1 ATE525087 T1 AT E525087T1 AT 06753791 T AT06753791 T AT 06753791T AT 06753791 T AT06753791 T AT 06753791T AT E525087 T1 ATE525087 T1 AT E525087T1
Authority
AT
Austria
Prior art keywords
ion exchange
purifying antibodies
immunoglobulin
purification
single step
Prior art date
Application number
AT06753791T
Other languages
English (en)
Inventor
Roberto Falkenstein
Burkard Kolb
Maria Smida
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34936911&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE525087(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE525087T1 publication Critical patent/ATE525087T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT06753791T 2005-05-25 2006-05-23 Verfahren zur aufreinigung von antikörpern ATE525087T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05011302 2005-05-25
PCT/EP2006/004863 WO2006125599A2 (en) 2005-05-25 2006-05-23 Method for the purification of antibodies

Publications (1)

Publication Number Publication Date
ATE525087T1 true ATE525087T1 (de) 2011-10-15

Family

ID=34936911

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06753791T ATE525087T1 (de) 2005-05-25 2006-05-23 Verfahren zur aufreinigung von antikörpern

Country Status (18)

Country Link
US (3) US20090098660A1 (de)
EP (2) EP2261252B2 (de)
JP (1) JP4852602B2 (de)
KR (1) KR100967778B1 (de)
CN (1) CN101180317B (de)
AT (1) ATE525087T1 (de)
AU (1) AU2006251307B2 (de)
BR (1) BRPI0610201B8 (de)
CA (1) CA2607375C (de)
DK (1) DK1888636T4 (de)
ES (2) ES2373402T5 (de)
FI (1) FI1888636T4 (de)
IL (2) IL186338A (de)
MX (1) MX2007014269A (de)
NO (1) NO345362B1 (de)
PL (1) PL1888636T5 (de)
TW (2) TWI391399B (de)
WO (1) WO2006125599A2 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
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TWI391399B (zh) 2005-05-25 2013-04-01 Hoffmann La Roche 測定溶離多肽之鹽濃度之方法
JP5064558B2 (ja) * 2007-06-01 2012-10-31 エフ．ホフマン−ラ ロシュ アーゲー 免疫グロブリン精製
CL2008002053A1 (es) * 2007-07-17 2009-05-22 Hoffmann La Roche Metodo para la purificacion de una eritropoyetina monopeguilada (epompeg) que consiste en proporcionar una solucion que contiene eritropoyetina mono, poli y no peguilada y hacerla pasar por dos pasos de cromatografia de intercambio cationico y metodo para producir epo mpeg que incluye metodo de purificacion.
AR067536A1 (es) 2007-07-17 2009-10-14 Hoffmann La Roche Metodo para obtener una eritropoyetina mono-pegilada en una forma sustancialmente homogenea
CN102171237A (zh) * 2008-08-14 2011-08-31 默沙东公司 使用蛋白a亲和色谱法纯化抗体的方法
WO2011110598A1 (en) 2010-03-10 2011-09-15 F. Hoffmann-La Roche Ag Method for purifying immunoglobulin solutions
CA2792717A1 (en) * 2010-04-14 2011-10-20 F. Hoffmann-La Roche Ag Immunoglobulin aggregate removal
US20120264920A1 (en) * 2010-10-11 2012-10-18 Abbott Laboratories Processes for purification of proteins
BR112013012422A2 (pt) 2010-12-21 2016-08-30 Hoffmann La Roche "método para produzir uma preparação de anticorpo e anticorpo anti-her2"
SI2686334T2 (sl) 2011-03-16 2020-10-30 F. Hoffmann-La Roche Ag Ionska izmenjevalna kromatografija z izboljšano selektivnostjo za ločevanje polipeptidnih monomerov, agregatov in fragmentov s spremembo mobilne faze
JP6292718B2 (ja) * 2011-07-01 2018-03-14 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 凝集ポリペプチドから単量体ポリペプチドを分離するための方法
US10481164B2 (en) * 2012-03-26 2019-11-19 Amgen Inc. Method for using light scattering in real time to directly monitor and control impurity removal in purification processes
SG11201602061YA (en) * 2013-09-17 2016-04-28 Kaneka Corp Novel antibody purification method and antibody obtained therefrom, and novel antibody purification method using cation exchanger and antibody obtained therefrom
US10351592B2 (en) * 2013-10-30 2019-07-16 Celltrion, Inc. Method for separating antibody isoforms using cation exchange chromatography
AR102521A1 (es) 2014-11-06 2017-03-08 Hoffmann La Roche Variantes de región fc con unión de fcrn modificada y métodos de utilización
GB2539420B (en) * 2015-06-16 2021-01-13 Cytiva Sweden Ab Determination of chromatography conditions
EP3377513A1 (de) * 2015-11-18 2018-09-26 Merck Patent GmbH Verbesserte proteintrennung in der ionenaustauschchromatografie
MX2018005831A (es) * 2015-11-18 2018-08-01 Merck Patent Gmbh Gradientes de ph-sal opuestos para separaciones de proteina mejoradas.
WO2019183334A1 (en) 2018-03-21 2019-09-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
CA3095850A1 (en) * 2018-04-03 2019-10-10 Merck Patent Gmbh Cex chromatography media and low salt elution of target proteins from biopharmaceutical feeds
CN111068361B (zh) * 2018-10-22 2022-02-11 中国石油化工股份有限公司 一种己内酰胺离子交换装置及其再生方法
CN110066314B (zh) * 2019-04-01 2023-10-27 上海药明生物技术有限公司 一种高效提高多聚体分离分辨率的亲和纯化工艺
TW202128264A (zh) * 2019-09-25 2021-08-01 瑞士商赫孚孟拉羅股份公司 使用經預測之溶離緩衝鹽濃度的陽離子層析法

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Also Published As

Publication number Publication date
EP1888636B1 (de) 2011-09-21
BRPI0610201A2 (pt) 2010-06-01
CA2607375A1 (en) 2006-11-30
IL186338A (en) 2011-01-31
TW200716669A (en) 2007-05-01
US11034754B2 (en) 2021-06-15
CN101180317A (zh) 2008-05-14
PL1888636T3 (pl) 2012-02-29
ES2373402T5 (es) 2022-09-14
US9631007B2 (en) 2017-04-25
WO2006125599A3 (en) 2007-06-21
ES2373402T3 (es) 2012-02-03
BRPI0610201B8 (pt) 2021-05-25
NO345362B1 (no) 2020-12-28
WO2006125599A2 (en) 2006-11-30
KR100967778B1 (ko) 2010-07-05
CA2607375C (en) 2014-01-21
DK1888636T3 (da) 2011-11-14
ES2389641T5 (es) 2021-06-10
EP1888636B2 (de) 2022-03-30
CN101180317B (zh) 2013-06-19
NO20075091L (no) 2008-01-23
JP4852602B2 (ja) 2012-01-11
US20170183394A1 (en) 2017-06-29
US20140243508A1 (en) 2014-08-28
TW200946543A (en) 2009-11-16
FI1888636T4 (fi) 2023-01-31
EP2261252A3 (de) 2011-03-23
IL186338A0 (en) 2008-01-20
PL1888636T5 (pl) 2023-03-06
AU2006251307B2 (en) 2011-08-04
EP2261252B1 (de) 2012-07-25
DK1888636T4 (da) 2022-07-18
EP2261252B2 (de) 2020-10-21
US20090098660A1 (en) 2009-04-16
TWI320788B (en) 2010-02-21
IL206993A0 (en) 2012-01-01
EP1888636A2 (de) 2008-02-20
KR20080017322A (ko) 2008-02-26
JP2008542218A (ja) 2008-11-27
EP2261252A2 (de) 2010-12-15
TWI391399B (zh) 2013-04-01
CA2607375F (en) 2006-11-30
MX2007014269A (es) 2008-01-24
ES2389641T3 (es) 2012-10-30
BRPI0610201B1 (pt) 2020-12-29
AU2006251307A1 (en) 2006-11-30

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